26,781 results match your criteria immunoprecipitation studies


CircNFIB inhibits tumor growth and metastasis through suppressing MEK1/ERK signaling in intrahepatic cholangiocarcinoma.

Mol Cancer 2022 Jan 17;21(1):18. Epub 2022 Jan 17.

Department of Liver Surgery & Liver Transplantation, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 610041, China.

Background: Considerable evidence shows that circular RNAs (circRNAs) play an important role in tumor development. However, their function in intrahepatic cholangiocarcinoma (ICC) metastasis and the underlying mechanisms are incompletely understood.

Methods: circNFIB (hsa_circ_0086376, termed as cNFIB hereafter) was identified in human ICC tissues through circRNAs sequencing. Read More

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January 2022

Abnormal 5-methylcytosine lncRNA methylome is involved in human high-grade serous ovarian cancer.

Am J Transl Res 2021 15;13(12):13625-13639. Epub 2021 Dec 15.

Department of Gynecology, The Second Hospital of Hebei Medical University 215 Heping West Road, Shijiazhuang 050011, Hebei, China.

Methylcytosine (m5C) is an important posttranscriptional RNA methylation modification. Studies have reported that aberrant RNA methylation can regulate tumorigenesis and development, indicating the importance of exploring the distribution and biological functions of m5C modification in human high-grade serous ovarian cancer (HGSOC) lncRNAs. In the current study, we identified 2,050 dysregulated m5C peaks, 1,767 of which were significantly upregulated, while 283 were significantly downregulated by performing methylated RNA immunoprecipitation sequencing on 3 pairs of human HGSOC tissues and paired normal tissues. Read More

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December 2021

Spreading-dependent or independent Sir2-mediated gene silencing in budding yeast.

Genes Genomics 2022 Jan 16. Epub 2022 Jan 16.

Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, 1 Kangwondeahak-gil, Chuncheon, 24341, Republic of Korea.

Background: In the budding yeast Saccharomyces cerevisiae, a silent chromatin structure is formed at three distinct loci, including telomeres, rDNA, and mating-type loci, which silence the expression of genes within their structures. Sir2 is the only common factor, regulating the three silent chromatin regions. S. Read More

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January 2022

A pro-endocrine pancreatic islet transcriptional program established during development is retained in human gallbladder epithelial cells.

Cell Mol Gastroenterol Hepatol 2022 Jan 12. Epub 2022 Jan 12.

Diabetes and Islet Biology Group, School of Medicine, Western Sydney University, 30 Narellan Road & Gilchrist Drive, Campbelltown, NSW 2560, Australia. Electronic address:

Background And Aims: Pancreatic islet β-cells are factories for insulin production; however ectopic expression of insulin is also well recognized. The gallbladder is a next-door neighbour to the developing pancreas. Here, we wanted to understand if gallbladders contain functional insulin-producing cells. Read More

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January 2022

Polycomb repressive complex 1 initiates and maintains tailless repression in Drosophila embryo.

Authors:
Gwo-Jen Liaw

Biochim Biophys Acta Gene Regul Mech 2022 Jan 12:194786. Epub 2022 Jan 12.

Department of Life Sciences and Institute of Genomic Sciences, National Yang Ming Chiao Tung University, Yangming Campus, No. 155, Sec. 2, Linong St., Taipei 112, Taiwan. Electronic address:

Maternally-deposited morphogens specify the fates of embryonic cells via hierarchically regulating the expression of zygotic genes that encode various classes of developmental regulators. Once the cell fates are determined, Polycomb-group proteins frequently maintain the repressed state of the genes. This study investigates how Polycomb-group proteins repress the expression of tailless, which encodes a developmental regulator in Drosophila embryo. Read More

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January 2022

Hippocampal Cannabinoid 1 Receptors Are Modulated Following Cocaine Self-administration in Male Rats.

Mol Neurobiol 2022 Jan 15. Epub 2022 Jan 15.

Laboratoire de Neurosciences Cognitives Et Adaptatives (LNCA), Centre de La Recherche Nationale Scientifique, Université de Strasbourg, 12 rue Goethe, 67000, Strasbourg, France.

Cocaine addiction is a complex pathology inducing long-term neuroplastic changes that, in turn, contribute to maladaptive behaviors. This behavioral dysregulation is associated with transcriptional reprogramming in brain reward circuitry, although the mechanisms underlying this modulation remain poorly understood. The endogenous cannabinoid system may play a role in this process in that cannabinoid mechanisms modulate drug reward and contribute to cocaine-induced neural adaptations. Read More

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January 2022

Sevoflurane represses the progression of glioma by the regulation of circ_0037655/miR-130a-5p/RPN2 axis.

Metab Brain Dis 2022 Jan 15. Epub 2022 Jan 15.

Department of Anesthesiology, Ganzhou People's Hospital, No.16, Meiguan Avenue, Zhanggong District, Ganzhou City, 341000, Jiangxi Province, China.

Sevoflurane (SEV) is a common anesthetic to inhibit glioma progression. The previous studies have indicated the molecular mechanisms of SEV function in glioma. The objective of this study was to explore the association of circ_00037655 with SEV in glioma. Read More

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January 2022

Fuchs Endothelial Corneal Dystrophy associated risk variant, rs3768617 in LAMC1 shows allele specific binding of GFI1B.

Gene 2022 Jan 11:146179. Epub 2022 Jan 11.

School of Biological Sciences, National Institute of Science Education and Research (NISER) Bhubaneswar, Homi Bhabha National Institute (HBNI), P.O. Bhimpur-Padanpur, Jatni, Khurda, Odisha, 752050, India. Electronic address:

Aims: To investigate the genetic and functional association of an intronic variant of LAMC1, rs3768617 with Fuchs endothelial corneal dystrophy (FECD) in the Indian population.

Methods: Blood samples were collected from age and sex matched 356 controls and 120 FECD patients after a detailed assessment via specular microscopy. Genomic DNA was extracted and genotyping was done by fluorescence based capillary electrophoresis. Read More

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January 2022

Medium-chain fatty acids enhance expression and histone acetylation of genes related to lipid metabolism in insulin-resistant adipocytes.

Biochem Biophys Rep 2022 Mar 5;29:101196. Epub 2022 Jan 5.

Graduate School of Life and Environmental Sciences, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.

Background: The expressions of genes related to lipid metabolism are decreased in adipocytes with insulin resistance. In this study, we examined the effects of fatty acids on the reduced expressions and histone acetylation of lipid metabolism-related genes in 3T3-L1 adipocytes treated with insulin resistance induced by tumor necrosis factor (TNF)-α.

Methods: Short-, medium-, and long-chain fatty acid were co-administered with TNF-α in 3T3-L1 adipocytes. Read More

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A non-canonical role for pyruvate kinase M2 as a functional modulator of Ca signalling through IP receptors.

Biochim Biophys Acta Mol Cell Res 2022 Jan 11;1869(4):119206. Epub 2022 Jan 11.

Division of Hematology/Oncology, Dept. Medicine, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA; Dept of Medicine, University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA; Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. Electronic address:

Pyruvate kinase isoform M2 (PKM2) is a rate-limiting glycolytic enzyme that is widely expressed in embryonic tissues. The expression of PKM2 declines in some tissues following embryogenesis, while other pyruvate kinase isozymes are upregulated. However, PKM2 is highly expressed in cancer cells and is believed to play a role in supporting anabolic processes during tumour formation. Read More

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January 2022

Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease.

Mol Ther Nucleic Acids 2022 Mar 29;27:256-275. Epub 2021 Nov 29.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.

Existing studies have revealed that microRNAs (miRNAs) have a role in cognitive deficits in Alzheimer's disease (AD). However, the function and pathophysiological mechanism of deregulated miRNAs underlying AD pathology remain to be investigated. The present study aimed to clarify the role and mechanism of miR-148a-3p in AD. Read More

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Pitfalls in Assessing Measurable Residual Disease in Plasma Cell Myeloma: a Comparison between Common Laboratory Tests.

Clin Lab 2022 Jan;68(1)

Background: Measurable residual disease (MRD) in plasma cell myeloma is one of the most important determinants for patients' outcome. Several laboratory tests exist to assess for the presence of MRD with variable accuracy. The aim of this study is to examine the sensitivity of immunofixation electrophoresis (IFE), serum free light chain (FLC), bone marrow immunohistochemistry (IHC), and multicolor flow cytometry (FC) and to address potential caveats of each test. Read More

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January 2022

Induction of cancer cell stemness in glioma through glycolysis and the long noncoding RNA HULC-activated FOXM1/AGR2/HIF-1α axis.

Lab Invest 2022 Jan 10. Epub 2022 Jan 10.

Department of Neurosurgery, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China.

Gliomas are the most common primary intracranial tumor, accounting for more than 70% of brain malignancies. Studies indicate that highly upregulated in liver cancer (HULC), a long noncoding RNA (lncRNA), functions as an oncogene in gliomas. However, the underlying mechanism of HULC in gliomas remains under-studied and was subsequently investigated in the current study. Read More

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January 2022

TdIF1-LSD1 Axis Regulates Epithelial-Mesenchymal Transition and Metastasis via Histone Demethylation of E-Cadherin Promoter in Lung Cancer.

Int J Mol Sci 2021 Dec 27;23(1). Epub 2021 Dec 27.

Institute of Immunotherapy, College of Basic Medicine, The First Affiliated Hospital of Nanchang University, Jiangxi Academy of Medical Sciences, Nanchang 330046, China.

We have previously found that TdT-interacting factor 1 (TdIF1) is a potential oncogene expressed in non-small cell lung cancer (NSCLC) and is associated with poor prognosis. However, its exact mechanism is still unclear. The lysine-specific demethylase 1 (LSD1) is a crucial mediator of the epithelial-mesenchymal transition (EMT), an important process triggered during cancer metastasis. Read More

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December 2021

The circ-PITX1 promotes non-small cell lung cancer development via the miR-30e-5p/ITGA6 axis.

Cell Cycle 2022 Jan 10:1-18. Epub 2022 Jan 10.

Department of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine, Nankai University, Tianjin, China.

Non-small cell lung cancer (NSCLC) is one of the most prevalent tumors with high incidence and mortality across the globe. Recently, increasing studies have demonstrated that circular RNAs (circRNAs) exert outstanding functions in NSCLC progression. Notwithstanding, we are still in the dark about the function and exact mechanism of circ-PITX1, a newly discovered circRNA. Read More

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January 2022

Acute sleep deprivation upregulates serotonin 2A receptors in the frontal cortex of mice via the immediate early gene Egr3.

Mol Psychiatry 2022 Jan 10. Epub 2022 Jan 10.

Department of Basic Medical Sciences, University of Arizona College of Medicine-Phoenix, 425 N. 5th St, Phoenix, AZ, 85004, USA.

Serotonin 2A receptors (5-HTRs) mediate the hallucinogenic effects of psychedelic drugs and are a key target of the leading class of medications used to treat psychotic disorders. These findings suggest that dysfunction of 5-HTRs may contribute to the symptoms of schizophrenia, a mental illness characterized by perceptual and cognitive disturbances. Indeed, numerous studies have found that 5-HTRs are reduced in the brains of individuals with schizophrenia. Read More

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January 2022

Puf-A promotes cancer progression by interacting with nucleophosmin in nucleolus.

Oncogene 2022 Jan 9. Epub 2022 Jan 9.

Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan.

Previously, we identified Puf-A as a novel member of Puf-family RNA-binding proteins; however, its biological functions remain obscure. Analysis of tumor samples of non-small cell lung cancer (NSCLC) showed that high Puf-A expression correlated with high histology grade and abnormal p53 status. Kaplan-Meier curve for overall survival revealed high expression of Puf-A to predict poor prognosis in stage I NSCLC. Read More

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January 2022

Corticosterone-mediated regulation and functions of miR-218-5p in rat brain.

Sci Rep 2022 Jan 7;12(1):194. Epub 2022 Jan 7.

Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.

Chronic stress is one of the key precipitating factors in major depressive disorder (MDD). Stress associated studies have underscored the mechanistic role of epigenetic master players like microRNAs (miRNAs) in depression pathophysiology at both preclinical and clinical levels. Previously, we had reported changes in miR-218-5p expression in response to corticosterone (CORT) induced chronic stress. Read More

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January 2022

INPP5F translocates into cytoplasm and interacts with ASPH to promote tumor growth in hepatocellular carcinoma.

J Exp Clin Cancer Res 2022 Jan 7;41(1):13. Epub 2022 Jan 7.

Department of Hepatobiliary Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.

Background: Increasing evidence has suggested inositol polyphosphate 5-phosphatase family contributes to tumorigenesis and tumor progression. However, the role of INPP5F in hepatocellular carcinoma (HCC) and its underlying mechanisms is unclear.

Methods: The expression of INPP5F in HCC was analyzed in public databases and our clinical specimens. Read More

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January 2022

Computational modeling of chromatin accessibility identified important epigenomic regulators.

BMC Genomics 2022 Jan 8;23(1):19. Epub 2022 Jan 8.

Department of Medicine, Baylor College of Medicine, Room ICTR 100D, One Baylor Plaza, Baylor College of Medicine, Houston, TX, 77030, USA.

Chromatin accessibility is essential for transcriptional activation of genomic regions. It is well established that transcription factors (TFs) and histone modifications (HMs) play critical roles in chromatin accessibility regulation. However, there is a lack of studies that quantify these relationships. Read More

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January 2022

Circ_0044516 Enriches the Level of SARM1 as a miR-330-5p Sponge to Regulate Cell Malignant Behaviors and Tumorigenesis of Prostate Cancer.

Authors:
Yan Wu

Biochem Genet 2022 Jan 7. Epub 2022 Jan 7.

Department of Urology, Section 4, People's Hospital of Hunan Province, No.89, Guhan Road, Furong District, Changsha, 410002, Hunan, China.

Prostate cancer (PCa) is one of the most common and deadly cancers in men. The dysregulated circular RNAs (circRNAs) are involved in the development of various cancers, including PCa. The purpose of this study was to construct a circRNA-microRNA (miRNA)-mRNA network to explain the function of circ_0044516 in PCa. Read More

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January 2022

Chromatin alterations during the epididymal maturation of mouse sperm refine the paternally inherited epigenome.

Epigenetics Chromatin 2022 01 6;15(1). Epub 2022 Jan 6.

Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4466, USA.

Background: Paternal lifestyle choices and male exposure history have a critical influence on the health and fitness of the next generation. Accordingly, defining the processes of germline programming is essential to resolving how the epigenetic memory of paternal experiences transmits to their offspring. Established dogma holds that all facets of chromatin organization and histone posttranslational modification are complete before sperm exits the testes. Read More

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January 2022

Circ_0007385 regulates cell proliferation, apoptosis and stemness via targeting miR-493-3p/RAB22A axis in non-small cell lung cancer.

Thorac Cancer 2022 Jan 5. Epub 2022 Jan 5.

Department of cardiothoracic surgery, People's Hospital of Beilun District, Ningbo, China.

Background: Non-small cell lung cancer (NSCLC) is a common cancer in the United States. Previous studies have shown that circular RNAs (circRNAs) can affect NSCLC progression, but its regulatory mechanism is still indistinct. In this study, we unfold the roles of circular RNA_0007385 in NSCLC tissues and cells. Read More

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January 2022

Hsa_circ_0003258 promotes prostate cancer metastasis by complexing with IGF2BP3 and sponging miR-653-5p.

Mol Cancer 2022 01 5;21(1):12. Epub 2022 Jan 5.

Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

Background: More and more studies have shown that circular RNAs (circRNAs) play a critical regulatory role in many cancers. However, the potential molecular mechanism of circRNAs in prostate cancer (PCa) remains largely unknown.

Methods: Differentially expressed circRNAs were identified by RNA sequencing. Read More

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January 2022

Integrative analysis of the 3D genome structure reveals that CTCF maintains the properties of mouse female germline stem cells.

Cell Mol Life Sci 2022 Jan 3;79(1):22. Epub 2022 Jan 3.

Renji Hospital, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.

The three-dimensional configuration of the genome ensures cell type-specific gene expression profiles by placing genes and regulatory elements in close spatial proximity. Here, we used in situ high-throughput chromosome conformation (in situ Hi-C), RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) to characterize the high-order chromatin structure signature of female germline stem cells (FGSCs) and identify its regulating key factor based on the data-driven of multiple omics data. By comparison with pluripotent stem cells (PSCs), adult stem cells (ASCs), and somatic cells at three major levels of chromatin architecture, A/B compartments, topologically associating domains, and chromatin loops, the chromatin architecture of FGSCs was most similar to that of other ASCs and largely different from that of PSCs and somatic cells. Read More

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January 2022

Developmental and Injury-induced Changes in DNA Methylation in Regenerative versus Non-regenerative Regions of the Vertebrate Central Nervous System.

BMC Genomics 2022 Jan 4;23(1). Epub 2022 Jan 4.

Department of Biological Sciences, University at Albany, State University of New York, Albany, NY, 12222, USA.

Background: Because some of its CNS neurons (e.g., retinal ganglion cells after optic nerve crush (ONC)) regenerate axons throughout life, whereas others (e. Read More

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January 2022

Unraveling three-dimensional chromatin structural dynamics during spermatogonial differentiation.

J Biol Chem 2021 Dec 31:101559. Epub 2021 Dec 31.

Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

Spermatogonial stem cells (SSCs) are able to undergo both self-renewal and differentiation. Unlike self-renewal, which replenishes the SSC and progenitor pool, differentiation is an irreversible process committing cells to meiosis. While the preparations for meiotic events in differentiating spermatogonia (Di-SG) are likely to be accompanied by alterations in chromatin structure, the three-dimensional (3D) chromatin architectural differences between SSCs and Di-SG, and the higher-order chromatin dynamics during spermatogonial differentiation, have not been systematically investigated. Read More

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December 2021

Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY.

Methods Mol Biol 2022 ;2423:39-50

Department of Pediatrics, UT MD Anderson Cancer Center, Houston, TX, USA.

Studies of DNA-protein interactions have revealed regulatory mechanisms of DNA replication, repair, remodeling, and transcription. Perturbation of any or all of these processes result in differential gene expression that can lead to tumor development. Chromatin immunoprecipitation assay (ChIP), currently the only method available to explore DNA-binding in vivo, has become a vastly utilized tool for cancer research. Read More

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January 2022

LncRNA UCA1 mediates Cetuximab resistance in Colorectal Cancer via the MiR-495 and HGF/c-MET Pathways.

J Cancer 2022 1;13(1):253-267. Epub 2022 Jan 1.

Department of Gastrointestinal Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang Province, China.

Cetuximab is one of the most widely used monoclonal antibodies to treat patients with RAS/BRAF wild-type metastatic colorectal cancer (mCRC). Unfortunately, cetuximab resistance often occurs during targeted therapy. However, the underlying epigenetic mechanisms remain unclear. Read More

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January 2022

CircRTN4IP1 regulates the malignant progression of intrahepatic cholangiocarcinoma by sponging miR-541-5p to induce HIF1A production.

Pathol Res Pract 2021 Dec 5;230:153732. Epub 2021 Dec 5.

Department of Interventional, Cancer Hospital Affiliated to Xinjiang Medical University, Urumqi, Xinjiang, China. Electronic address:

Background: Recent studies indicate that circular RNA (circRNA) serves important roles in the development of intrahepatic cholangiocarcinoma (ICC). However, the role of circRNA reticulon 4 interacting protein 1 (circRTN4IP1) in ICC progression remains unknown.

Methods: Expression of circRTN4IP1, microRNA-541-5p (miR-541-5p), hypoxia inducible factor 1 subunit alpha (HIF1A) and other indicated protein markers was detected by quantitative real-time polymerase chain reaction or Western blot. Read More

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December 2021