3,096 results match your criteria immunochromatographic testsconclusions

Comparison of Immunochromatographic Test (ICT) and Filariasis Test Strip (FTS) for Detecting Lymphatic Filariasis Antigen in American Samoa, 2016.

Trop Med Infect Dis 2021 Jul 14;6(3). Epub 2021 Jul 14.

College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns 4870, Australia.

Circulating filarial antigen (Ag) prevalence, measured using rapid point-of-care tests, is the standard indicator used for monitoring and surveillance in the Global Program to Eliminate Lymphatic Filariasis. In 2015, the immunochromatographic test (ICT) was replaced with the filariasis test strip (FTS), which has higher reported sensitivity. Despite differences in sensitivity, no changes in recommended surveillance targets were made when the FTS was introduced. Read More

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Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens.

Diagn Microbiol Infect Dis 2021 Jun 20;101(3):115459. Epub 2021 Jun 20.

Dipartimento di Scienze per la Promozione della Salute e Materno Infantile "G. D'Alessandro", Palermo, Italy. Electronic address:

The performance of 2 antigenic commercial assays for enteric adenovirus (AdV) infection, bioNexia Rota-Adeno ImmunoChromatographic Tests (ICT) and LIAISON® Adenovirus ChemiLuminescence Immuno Assays (CLIA), was evaluated on 321 stools from children hospitalized for acute gastroenteritis in Palermo, Italy, using a Real time-PCR (Rt-PCR) as reference method. The CLIA showed higher sensitivity (77% vs 60%), accuracy (94.4 vs 90. Read More

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Emergent canine visceral leishmaniasis in Argentina: Comparative diagnostics and relevance to proliferation of human disease.

PLoS Negl Trop Dis 2021 Jul 19;15(7):e0009552. Epub 2021 Jul 19.

Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.

Background: Visceral leishmaniasis (VL) is a zoonotic protozoal vector-borne disease that is a major public health challenge. In Argentina, canine (CVL) and human visceral leishmaniasis (HVL) have recently emerged. There is a lack of standardised diagnostic tests for CVL, which hinders control of CVL and HVL. Read More

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Red ZnSiO:Eu and MgTiO:Mn nanophosphors for on-site rapid optical detections: Synthesis and characterization.

Appl Phys A Mater Sci Process 2021 10;127(8):588. Epub 2021 Jul 10.

Department of Electronic Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 807 Taiwan, ROC.

This study reports the synthesis and characterization of the red nanophosphors ZnSiO:Eu (ZSO:Eu) and MgTiO:Mn (MTO:Mn). The use of phosphors as a fluorescence label for lateral flow immunochromatographic assay (LFIA) has also been described. The optimal photoluminescence (PL) for ZSO:Eu was obtained when it was synthesized with 7 mol% of Eu and annealed at 1100 °C for 1 h. Read More

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Case Report: Angiostrongylus cantonesis Myelitis in Thailand.

Am J Trop Med Hyg 2021 Jul 16. Epub 2021 Jul 16.

Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

A 67-year-old man presented with headache, middle back pain that radiated to both legs, and paresthesia in the right leg for 1 day. He had eaten raw shrimp 1 week previously. Over the next week after admission, he developed urinary retention and weakness in both legs. Read More

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Immunochromatographic assay based on high-affine monoclonal antibody for the detection of imidocarb in milk.

J Food Sci 2021 Jul 15. Epub 2021 Jul 15.

School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

Imidocarb (IM) is an antiprotozoal agent, which is mainly used to treat babesiosis and piroplasmosis for animals. However, overdose or improbable utilization cause IM residues in animal origin products, which would be harmful to human health. Here, a monoclonal antibody (mAb) against IM with extremely sensitive and specific features has been successfully prepared from a novel immunogen synthesized by virtue of the active ester method. Read More

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Carbapenemase detection by NG-Test CARBA 5-a rapid immunochromatographic assay in carbapenem-resistant diagnosis.

Ann Transl Med 2021 May;9(9):769

Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The global spread of carbapenem-resistant (CRE) represents a serious public health concern as these organisms are associated with limited treatment options, high mortality rate and rapid transmissibility. The identification of carbapenemase remains a challenge in microbiological laboratories as no single method is perfect when considering cost, carbapenemase coverage, accuracy, handling complexity and TATs together.

Methods: NG-Test CARBA 5 assay and modified carbapenem inactivation method in conjunction with EDTA carbapenem inactivation method (mCIM/eCIM) were challenged with a collection of 299 molecularly characterized CRE isolates in China in order to evaluate the performance in detecting five major carbapenemases ( , , , , and ) among Enterobacterales. Read More

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Evaluation of Rapid Antibody Test and Chest Computed Tomography Results of COVID-19 Patients: A Retrospective Study.

J Med Virol 2021 Jul 14. Epub 2021 Jul 14.

Department of Infectious Diseases and Clinical Microbiology, Nigde Omer Halisdemir University Training and Research Hospital, Nigde, Turkey.

Objective: The purpose of this study was to evaluate the SARS-CoV-2 IgM/IgG rapid antibody test results in symptomatic patients with COVID-19 and their chest computed tomography (CT) data.

Methods: A total of 320 patients admitted to our hospital for different durations due to COVID-19 were included in the study. Serum samples were obtained within 0 to 7 days from COVID-19 patients confirmed by RT-PCR and chest CT scan. Read More

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Comparative assessment of immunochromatographic test kits using somatic antigens from adult Opisthorchis viverrini and IgG and IgG4 conjugates for serodiagnosis of human opisthorchiasis.

Parasitol Res 2021 Jul 14. Epub 2021 Jul 14.

Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine and Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, 40002, Thailand.

Chronic infections of humans with Opisthorchis viverrini and Clonorchis sinensis spanning decades may lead to life-threatening pathology prior to cholangiocarcinoma (CCA), which usually has a poor prognosis. Serological tools can support the parasitological examination in clinical diagnosis and support screening for risk of CCA. We developed novel immunochromatographic test kits using a soluble, somatic tissue extract of adult O. Read More

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Ultrasensitive immunochromatographic strip assay for the detection of diminazene.

Analyst 2021 Jul 13. Epub 2021 Jul 13.

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

We prepared monoclonal antibodies (mAbs) against diminazene and used them in the development of a gold nanoparticle-based lateral-flow test (GNT) strip and indirect competitive enzyme-linked immunosorbent assay for the detection of diminazene in beef and beef liver samples. MAbs, which belong to the IgG2a subclass, had a half maximal inhibitory concentration of 0.04 ng mL-1. Read More

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Epidemiology of SARS-CoV-2 Infection Evaluated by Immunochromatographic Rapid Testing for the Determination of IgM and IgG Against SARS-CoV-2 in a Cohort of Mask Wearing Workers in the Metal-Mechanical Sector in an Area With a High Incidence of COVID-19.

Front Public Health 2021;9:628098. Epub 2021 Jun 25.

University of Milan, Milan, Italy.

Although the diagnosis of new coronavirus 2019 (COVID-19) is made through the identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in respiratory secretions by means of molecular methods, a more accurate estimation of SARS-CoV-2 circulation can be obtained by seroprevalence studies. The main aim of this study was to evaluate the true epidemiology of SARS-CoV-2 infection among workers in the metal-mechanical sector who never stopped working during the pandemic period in an area with a high incidence of COVID-19 and to define whether and how they could continue the work without appreciable risks during a second wave. A total of 815 metal-mechanical workers who had never stopped working even during the pandemic period in three different factories in the Emilia-Romagna Region, Italy, and who had always used face masks during working hours, underwent a capillary blood rapid test for the determination of IgM and IgG against SARS-CoV-2 (COVID-19 IgG/IgM Rapid test, PrimaLab, Modena, Italy). Read More

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Novel phage display-derived recombinant antibodies recognizing both MPT64 native and mutant (63-bp deletion) are promising tools for tuberculosis diagnosis.

Biologicals 2021 Jul 8. Epub 2021 Jul 8.

Federal University of Pelotas, Technological Development Center, Pelotas, Brazil.

Tuberculosis (TB) is one of the top 10 causes of death in humans worldwide. The most important causative agents of TB are bacteria from the Mycobacterium tuberculosis complex (MTC), although nontuberculous mycobacteria (NTM) can also cause similar infections. The ability to identify and differentiate MTC isolates from NTM is important for the selection of the correct antimicrobial therapy. Read More

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Seroprevalence of antibodies against SARS-CoV-2 virus in Northern Serbia (Vojvodina): A four consecutive sentinel population-based survey study.

PLoS One 2021 9;16(7):e0254516. Epub 2021 Jul 9.

Department of Epidemiology, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia.

Background: Monitoring changes of seroprevalence over time is important at the beginning and during of COVID-19 outbreak to anticipate its dynamics and plan an adequate public health response.

Methods: We conducted a repeated cross-sectional investigation among asymptomatic outpatient subjects and covered 0.1% of total population of Northern Serbia (Autonomous Province of Vojvodina). Read More

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Cryptococcal antigen carriage among HIV infected children aged 6 months to 15 years at Laquintinie Hospital in Douala.

PLoS One 2021 9;16(7):e0253781. Epub 2021 Jul 9.

Department of Pediatrics, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.

Background: Up to 15% of deaths of people living with HIV is attributable to meningeal cryptococcosis, with nearly 75% occuring in sub-Saharan Africa. Although rare in children, it is a major cause of morbidity and mortality in people living with HIV. A strong association between cryptococcal antigenemia and the development of meningeal cryptococcosis has been shown in adults. Read More

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Diagnostic Accuracy of Immunochromatographic Tests for the Detection of Norovirus in Stool Specimens: a Systematic Review and Meta-Analysis.

Microbiol Spectr 2021 Jul 7:e0046721. Epub 2021 Jul 7.

Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.

Noroviruses are the leading cause of acute gastroenteritis in all age groups and constitute a major health and economic burden worldwide. This systematic review and meta-analysis aimed to determine the diagnostic accuracy of immunochromatographic tests (ICTs) for the detection of norovirus in stool specimens, which has not been performed previously. In this systematic review and meta-analysis (registered on PROSPERO, CRD42020186911), we searched Medline/PubMed, Embase, Cochrane Library, and Web of Science for all studies published up to 16 May 2020. Read More

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Cost-effectiveness analysis and budgetary impact of the Cryptococcal Antigen Lateral Flow Assay (CRAG-LFA) implementation for the screening and diagnosis of cryptococcosis in asymptomatic people living with HIV in Brazil.

Rev Inst Med Trop Sao Paulo 2021 5;63:e57. Epub 2021 Jul 5.

Universidade Federal Fluminense, Instituto de Saúde Coletiva, Departamento de Saúde e Sociedade, Niterói, Rio de Janeiro, Brazil.

Cryptococcal infection is a frequent cause of mortality in Brazilian HIV-infected patients. The present study aimed to evaluate the cost-effectiveness and budgetary impact of four cryptococcosis screening strategies in HIV-infected patients with CD4+ ≤ 200 cells/mm3, in Brazil. A Monte Carlo microsimulation was used to compare the following diagnostic tests: a systematic serum cryptococcal antigen (CRAG) screening with latex agglutination (CRAG-LA), a lateral flow immunochromatographic test (CRAG-LFA), India ink staining and no intervention. Read More

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Well-orientation strategy for direct binding of antibodies: Development of the immunochromatographic test using the antigen modified FeO nanoprobes for sensitive detection of aflatoxin B.

Food Chem 2021 Mar 21;364:129583. Epub 2021 Mar 21.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:

One of the major concerns in the application of nanocarriers in biosensing is the impair of the recognition molecules bioactivity loaded on their surfaces due to harsh and laborious cross-linking and random orientation, resulting in unsatisfactory sensitivity. Herein, we proposed a novel immunochromatographic test strip (FNS-ag-DICTS) by taking advantage of the antigen (ag) modified FeO nanostructures (FNSs) as new signal tags and goat anti-mouse IgG labeling on the detection line instead of ag, which was used for sensitive detection of aflatoxin B (AFB). The fabricated FNS-ag can orientate the Fab region of monoclonal antibodies (mAbs), waiving the intrinsic limitations of traditional nanomaterials labeled mAbs. Read More

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A colloidal gold-based immunochromatographic strip for rapid detection of SARS-CoV-2 antibodies after vaccination.

Med Nov Technol Devices 2021 Sep 26;11:100084. Epub 2021 Jun 26.

Interdisciplinary Institute of Cancer Diagnosis and Treatment, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, China.

Vaccination interventions is consideredan important preventive measure to block the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and protect the organism from pathogen infection effectively. However, a quick and accurate technique to evaluate the immune efficacy of the SARS-CoV-2 inactivated vaccine remains scarce. In this paper, an IgM-IgG antibody combined detection colloidal gold immunochromatography assay kit was optimized and developed, which can assess the efficacy of the inactivated SARS-CoV-2 vaccine. Read More

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September 2021

Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus.

Sens Actuators B Chem 2021 Oct 29;345:130372. Epub 2021 Jun 29.

Beijing Institute of Radiation Medicine, Beijing, 100850, PR China.

Rapid and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus (FluA) antigens in the early stages of virus infection is the key to control the epidemic spread. Here, we developed a two-channel fluorescent immunochromatographic assay (ICA) for ultrasensitive and simultaneous qualification of the two viruses in biological samples. A high-performance quantum dot nanobead (QB) was fabricated by adsorption of multilayers of dense quantum dots (QDs) onto the SiO surface and used as the highly luminescent label of the ICA system to ensure the high-sensitivity and stability of the assay. Read More

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October 2021

Seroprevalence of Brucella canis in canines from a dog shelter in Bogotá, Colombia

Biomedica 2021 06 29;41(2):260-270. Epub 2021 Jun 29.

Facultad de Medicina Veterinaria, Fundación Universitaria Agraria de Colombia, Bogotá, D.C., Colombia.

Introduction: The risk of Brucella canis infection in humans and dogs has increased due to the permanent exposure to asymptomatic carrier dogs. In Colombia, there is evidence of B. canis infection in humans living with dogs. Read More

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Immunological Cross-Reactivity of Proteins Extracted from the Oomycete and the Fungus Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis.

J Fungi (Basel) 2021 Jun 11;7(6). Epub 2021 Jun 11.

Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.

Pythiosis, a life-threatening disease caused by , has been increasingly diagnosed worldwide. A recently developed immunochromatographic test (ICT) enables the rapid diagnosis of pythiosis. During the 3-year clinical implementation of ICT in Thailand, we collected the laboratory reports of 38 animals with suspected pythiosis and detected ICT false-positive results in three horses and a dog with basidiobolomycosis. Read More

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Assessment of a Rapid Semi-Quantitative Immunochromatographic Test for the Evaluation of Transfer of Passive Immunity in Calves.

Animals (Basel) 2021 Jun 1;11(6). Epub 2021 Jun 1.

RumeXperts, 4317 Faimes, Belgium.

Calves are born agammaglobulinemic and they rely on transfer of passive immunity (TPI) through ingestion of colostrum from the dam. Ensuring the effectiveness of TPI through blood serum immunoglobulins (IgG) quantification is of critical importance for the prevention of calf diseases. The main objective of this study was to assess the performance of a novel on-farm immunochromatographic quick assay (SmartStrips, Bio-X Diagnostics, Rochefort, Belgium) compared to the ELISA reference method to directly measure serum IgG concentration and assess TPI status in beef and dairy calves. Read More

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Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B in Maize.

Biosensors (Basel) 2021 Jun 20;11(6). Epub 2021 Jun 20.

Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.

Aflatoxin B (AFB), a mycotoxin, is hepatotoxic, carcinogenic, and nephrotoxic in humans and animals, and contaminate a wide range of maize. In this study, an immunochromatographic assay (ICA) based on polystyrene microspheres (PMs) was developed for sensitive and quantitative detection of AFB in maize. The amounts of PMs, the condition for activating carboxyl groups of PMs, the amount of monoclonal antibody (mAb), and the volume of the immune probe were optimized to enhance the performance PMs-ICA for point-of-care testing of AFB in maize. Read More

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Portable, Rapid, and Sensitive Time-Resolved Fluorescence Immunochromatography for On-Site Detection of Dexamethasone in Milk and Pork.

Foods 2021 Jun 10;10(6). Epub 2021 Jun 10.

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

Dexamethasone (DEX) is widely used because of its anti-inflammatory, anti-endotoxin, anti-shock, and stress-enhancing response activities. It can increase the risk of diabetes and hypertension if it is abused or used improperly. However, there is a lack of sensitive and rapid screening methods for DEX in food. Read More

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Effective Diagnosis of Foot-And-Mouth Disease Virus (FMDV) Serotypes O and A Based on Optical and Electrochemical Dual-Modal Detection.

Biomolecules 2021 Jun 5;11(6). Epub 2021 Jun 5.

Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea.

Foot-and-mouth disease virus (FMDV) is a highly contagious disease that affects cloven-hoofed animals. The traditional diagnostic methods for FMDV have several drawbacks such as cross-reactivity, low sensitivity, and low selectivity. To overcome these drawbacks, we present an optical and electrochemical dual-modal approach for the specific detection of FMDV serotypes O and A by utilizing a magnetic nanoparticle labeling technique with resorufin β-d-glucopyranoside (res-β-glc) and β-glucosidase (β-glc), without the use of typical lateral flow assay or polymerase chain reaction. Read More

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Development of an antigen-ELISA and a colloidal gold-based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses.

J Vet Diagn Invest 2021 Jun 24:10406387211027538. Epub 2021 Jun 24.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold-based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e. Read More

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Development of a lateral-flow ICA strip for the detection of colchicine.

Anal Methods 2021 Jul 22;13(27):3092-3100. Epub 2021 Jun 22.

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

Colchicine (COL), which is extracted from colchicine and papaya, is widely used in medicine. However, COL poisoning causes serious adverse complications including death. Therefore, there is a need to develop a sensitive COL detection method. Read More

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A rapid test for the environmental detection of pigeon antigen.

Sci Total Environ 2021 Sep 15;788:147789. Epub 2021 May 15.

Servicio de Neumología, Departamento de Medicina, Hospital Universitario Vall d'Hebron, Universidad Autónoma de Barcelona, Cataluña, Spain; CIBER Enfermedades Respiratorias (Ciberes), Madrid, Spain.

Introduction: Avoidance of inhaled bird antigens is essential to prevent hypersensitivity pneumonitis disease progression. The aim of the present study was to develop a sandwich enzyme link immunoassay (ELISA) and an immunochromatographic test (ICT) and compare their ability to detect pigeon antigens in environmental samples.

Methods: An amplified sandwich ELISA using pigeon serum as a calibration standard and a ICT using gold-labeled anti-pigeon serum antibodies for the rapid detection of pigeon antigens in environmental samples were developed. Read More

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September 2021

Systematic comparison of three commercially available combination disc tests and zCIM for carbapenemase detection in Enterobacterales isolates.

J Clin Microbiol 2021 Jun 16:JCM0314020. Epub 2021 Jun 16.

University of Cologne, Faculty of Medicine and University Hospital Cologne, Institute for Medical Microbiology, Immunology and Hygiene, Cologne, Germany.

Detection of carbapenemases in Enterobacterales is crucial for patient treatment and infection control. Among others, combination disk tests (CDT) with different inhibitors (e.g. Read More

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Ultrasensitive immunochromatographic strip for the detection of cyhalothrin in foods.

Anal Methods 2021 Jul 16;13(27):3040-3049. Epub 2021 Jun 16.

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

In this study, a highly specific and sensitive monoclonal antibody (mAb) against lambda-cyhalothrin (LCT) was prepared. The half maximal inhibitory concentration (IC) in the ic-ELISA was 1.2 ng mL and the limit of detection (LOD) value was 0. Read More

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