80,474 results match your criteria immature

Morphometric properties of immature reticulocytes in health and during acute lymphoblastic and acute myeloid leukemia.

Tissue Cell 2021 Jun 10;71:101578. Epub 2021 Jun 10.

Department of Biology, Faculty of Biology and Chemistry, Belgorod State University, 85 Pobeda St., Belgorod, 308015, Russia. Electronic address:

Despite significant advances, many changes occurring in the tumor microenvironment during acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) remain unclear. The surface of immature reticulocytes was examined by atomic force microscopy (AFM) to determine specific changes during the development of ALL and AML. In patients with ALL the surface area of reticulocytes increased by 18. Read More

View Article and Full-Text PDF

Functional and radiological outcome after forearm plating in children and adolescent fracture.

Acta Orthop Belg 2021 Mar;87(1):143-149

The literature on forearm overgrowth after plating in traumatic conditions is relatively poor though this technique can be useful when intra-medullary nailing is not sufficient in pediatric cases. The goal of this study was to assess a potential overgrowth after plating and identify impact on function. We conducted a retrospective study of all pediatric patients who underwent open surgery of the radius and/or ulna diaphysis with internal fixation by plating, in our institution, between October 2013 and July 2019. Read More

View Article and Full-Text PDF

Effect of Tractor Speed and Spray Application Volume on Severity of Scab at Different Heights in the Canopy of Tall Pecan Trees.

Plant Dis 2021 Jun 15. Epub 2021 Jun 15.

Byron, United States;

Scab (caused by Venturia effusa) is the most important yield-limiting disease of pecan in the southeastern USA. On susceptible cultivars, the disease is managed using fungicides, but spray coverage is an issue in tall trees. In four experiments we used an air-blast sprayer to compare scab severity on fruit at 5. Read More

View Article and Full-Text PDF

MDSCs interactions with other immune cells and their role in maternal-fetal tolerance.

Int Rev Immunol 2021 Jun 15:1-18. Epub 2021 Jun 15.

Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, China.

MDSCs (myeloid-derived suppressor cells) are a population of immature and heterogeneous bone marrow cells with immunosuppressive functions, and they are mainly divided into two subgroups: granulocytic MDSCs (G-MDSCs) and monocytic MDSCs (M-MDSCs). Immunosuppression is the main and most important function of MDSCs, and they mainly exert an inhibitory effect through endoplasmic reticulum stress and some enzymes related to inhibitors, as well as some cytokines and other factors. In addition, MDSCs also interact with other immune cells, especially NK cells, DCs and Tregs, to participate in immune regulation. Read More

View Article and Full-Text PDF

A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes.

Chem Sci 2020 Apr 30;11(19):4999-5006. Epub 2020 Apr 30.

Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Science, Hokkaido University N21 W11, Kita-ku Sapporo 001-0021 Japan

Aberrantly truncated immature -glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis. Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature -glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently. Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis. Read More

View Article and Full-Text PDF

Autograft long head biceps tendon can be used as a scaffold for biologically augmenting rotator cuff repairs.

Arthroscopy 2021 Jun 11. Epub 2021 Jun 11.

Laboratory of Orthopaedic Tissue Regeneration & Orthobiologics, Department of Bioengineering, Clemson University, Clemson, SC; Frank H. Stelling and C. Dayton Riddle Orthopaedic Education and Research Laboratory, Clemson University Biomedical Engineering Innovation Campus, Greenville, SC.

Purpose: To create a viable, mechanically expanded autograft long head biceps tendon (LHBT) scaffold for biologically augmenting the repair of torn rotator cuffs.

Methods: The proximal aspect of the tenotomized LHBTs were harvested from patients during rotator cuff repair surgery and were mechanically formed into porous scaffolds using a surgical graft expander. LHBT scaffolds were evaluated for change in area, tensile properties, and tenocyte viability before and after expansion. Read More

View Article and Full-Text PDF

Differential susceptibility of rat primary neurons and neural stem cells to PFOS and PFOA toxicity.

Toxicol Lett 2021 Jun 11;349:61-68. Epub 2021 Jun 11.

Science for Life Laboratory, Department of Environmental Science, Stockholm University, Stockholm, 114 18, Sweden.

Per and polifluorinated substances (PFAS) are ubiquitous and persistent contaminants. Studies have indicated that fetuses and infants can be exposed to these chemicals in utero and through breastfeeding. Despite this, limited data about their effects on brain development are available. Read More

View Article and Full-Text PDF

Increased maturation of iPSC-derived neurons in a hydrogel-based 3D culture.

J Neurosci Methods 2021 Jun 11;360:109254. Epub 2021 Jun 11.

Institute for Regenerative Medicine, University of Zurich, Schlieren, Switzerland; Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland. Electronic address:

Background: Induced pluripotent stem cells (iPSCs) can be differentiated into virtually every desired cell type, offering significant potential for modeling human diseases in vitro. A disadvantage is that iPSC-derived cells represent an immature, which presents a major limitation for modeling age-related diseases such as Alzheimer's disease. Evidence suggests that culturing iPSC neurons in a 3D environment may increase neuronal maturity. Read More

View Article and Full-Text PDF

GmhB enzyme involved in ADP-heptose biosynthesis pathway is essential for lipopolysaccharide biosynthesis and bacterial virulence.

Virulence 2021 Dec;12(1):1610-1628

Institute of Molecular Medicine, College of Life Science, National Tsing Hua University, Hsinchu, Taiwan.

infection is linked to serious gastric-related diseases including gastric cancer. However, current therapies for treating infection are challenged by the increased antibiotic resistance of . Therefore, it is in an urgent need to identify novel targets for drug development against infection. Read More

View Article and Full-Text PDF
December 2021

Oocyte stimulation parameters influence the number and proportion of mature oocytes retrieved in assisted reproductive technology cycles.

J Assist Reprod Genet 2021 Jun 14. Epub 2021 Jun 14.

NYU Grossman School of Medicine, New York University Langone Fertility Center, New York, NY, 10016, USA.

Purpose: Whether differences in stimulation parameters alter the number and proportion of MII oocytes retrieved.

Methods: Records of 2546 patients were examined, looking at age, day 2/3 follicle-stimulating hormone (FSH) and estradiol (E2) levels, total dose of gonadotropins administered (including FSH and human menopausal gonadotropin [hMG]), fraction of hMG administered, number of days of treatment with gonadotropins, and the dose of gonadotropins administered per day. We segregated the patients into 3 different classes depending on the trigger method used and 2 groups based on egg freeze vs. Read More

View Article and Full-Text PDF

Experimental Simulation of Hydrocarbon Expulsion in Semi-open Systems from Variable Organic Richness Source Rocks.

ACS Omega 2021 Jun 24;6(22):14664-14676. Epub 2021 May 24.

School of Geosciences, China University of Petroleum, Qingdao, Shandong 266580, P. R. China.

To better understand oil and gas generation and expulsion mechanisms and their controlling factors, two-stage heating program (20 and 5 °C/d) at 11 target temperatures (250-580 °C) have been performed in a semi-open reactor on nine immature lacustrine shale samples from the Triassic Yanchang Formation in Ordos Basin, NW China, with total organic carbon (TOC) contents ranging from 0.5% to 30.0%. Read More

View Article and Full-Text PDF

Voltage-Gated Sodium Channel () Mutation-Based Pyrethroid Resistance in Populations of Three Endemic Dengue Risk Areas of Sri Lanka.

Biomed Res Int 2021 22;2021:8874092. Epub 2021 May 22.

Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka.

Background: Pyrethroid insecticides are widely used in many countries for chemical-based control of . Regardless of their efficacy, the constant use of insecticides has induced insecticide resistance mechanisms, such as knockdown resistance () in mosquitoes. Sri Lankan Vector Controlling Entities (VCE) have been using a variety of pyrethroid insecticides as the primary approach for dengue control. Read More

View Article and Full-Text PDF

Vitamin E and selenium improve mesenchymal stem cell conditioned media immunomodulatory effects.

Stem Cell Investig 2021 7;8. Epub 2021 May 7.

Department of Medical Immunology, School of Medicine, Iran University of Medical Science, Tehran, Iran.

Background: Mesenchymal stem cells (MSCs) with immunoregulatory properties affect immune systems. Many studies showed that antioxidants such as vitamin E (Vit E) and selenium (Se) could improve stem cells survival. This study aims to investigate the effects of MSC conditioned media (CM) treated with Vit E and Se on immune cells. Read More

View Article and Full-Text PDF

Optimization of enzymatic hydrolysis of immature citrus ( Marcov.) for flavonoid content and antioxidant activity using a response surface methodology.

Food Sci Biotechnol 2021 May 7;30(5):663-673. Epub 2021 May 7.

Department of Food and Nutrition, Daegu University, Gyeonsan-si, 38453 Gyeongsangbukdo Korea.

Immature citrus with peel was enzymatically treated for production of a hydrolysate with enriched bioactive components and higher antioxidant activity. The effects of reaction factors, including enzyme concentration, reaction time, and temperature on hesperetin and naringenin contents, total phenolic content (TPC), and antioxidant activity were investigated using response surface methodology. The models were adequate, and the enzyme concentration, temperature, and time positively affected hesperetin and naringenin contents and TPC, but negatively affected DPPH radical scavenging capacity. Read More

View Article and Full-Text PDF

Management and Follow-Up of Complicated Crown Fractures with Intrusive Luxation of Maxillary Incisors in an 8-Year-Old Boy.

Case Rep Dent 2021 23;2021:5540860. Epub 2021 May 23.

Department of Pediatric Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

Intrusive luxation is a severe form of dental injury which causes damage to the pulp and supporting structures of a tooth because of its dislocation into the alveolar process. This paper shows the case of the reeruption of maxillary incisors accompanied by complicated crown fractures after 3 months. An 8-year-old boy patient was referred to the Department of Pedodontic Dentistry of Tehran University of Medical Science, Tehran, Iran, 18 hours after a fall at school. Read More

View Article and Full-Text PDF

Systemic Characterization of Novel Immune Cell Phenotypes in Recurrent Pregnancy Loss.

Front Immunol 2021 28;12:657552. Epub 2021 May 28.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Recurrent pregnancy loss (RPL) is a disturbing disease in women, and 50% of RPL is reported to be associated with immune dysfunction. Most previous studies of RPL focused mainly on the relationship between RPL and either T cells or natural killer (NK) cells in peripheral blood and the decidua; few studies presented the systemic profiles of the peripheral immune cell subsets in RPL women. Herein, we simultaneously detected 63 immune cell phenotypes in the peripheral blood from nonpregnant women (NPW), women with a history of normal pregnancy (NP) and women with a history of RPL (RPL) by multi-parameter flow cytometry. Read More

View Article and Full-Text PDF

Sex Differences in the Immune System Become Evident in the Perinatal Period in the Four Core Genotypes Mouse.

Front Endocrinol (Lausanne) 2021 27;12:582614. Epub 2021 May 27.

Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA, United States.

We have used the four core genotypes (FCG) mouse model, which allows a distinction between effects of gonadal secretions and chromosomal complement, to determine when sex differences in the immune system first appear and what influences their development. Using splenic T cell number as a measure that could be applied to neonates with as yet immature immune responses, we found no differences among the four genotypes at postnatal day 1, but by day 7, clear sex differences were observed. These sex differences were unexpectedly independent of chromosomal complement and similar in degree to gonadectomized FCG adults: both neonatal and gonadectomized adult females (XX and XY) showed 2-fold the number of CD4+ and 7-fold the number of CD8+ T cells their male (XX and XY) counterparts. Read More

View Article and Full-Text PDF

Personality and Lockdown: A Study on Italian Undergraduates During the COVID-19 Pandemic.

Front Psychiatry 2021 28;12:622366. Epub 2021 May 28.

Department of Human Neuroscience, Sapienza University of Rome, Rome, Italy.

The current study aimed at increasing our understanding of the psychological impact of the COVID-19 lockdown on undergraduate students, particularly with respect to the association between personality traits; defense mechanisms (DMs); depression, anxiety, and stress symptoms (DASSs); and compliance with the government recommended health measures. A sample of 1,427 Italian undergraduate students were administered the Personality Inventory for the DSM-5-Brief Form; the Defense Style Questionnaire-40; and the Depression, Anxiety and Stress Scale-21. Compliance with the COVID-19 behavioral recommendations was measured through a 10-item survey measure. Read More

View Article and Full-Text PDF

Study on ascending and descending vertical dispersal behavior of third instar larvae of (Fabricius) (Diptera:Calliphoridae): An evidence that blowflies survive burial.

Saudi J Biol Sci 2021 Jun 31;28(6):3176-3182. Epub 2021 Mar 31.

University of Security Management in Košice, Slovakia.

Although the pupation behavior of blowflies has been widely studied, this preliminary study was done on the vertical dispersal behavior (both ascending and descending) and fly emergence rate of third instar larvae of (Fabricius) to evaluate weather immature stages of blowflies survive burial and emerge out as adult. Third instar larvae of were placed at three different depths (5 cm, 25 cm and 45 cm) of soil under laboratory conditions to determine the impact of soil depth on the ascending and descending vertical dispersal behavior and the subsequent emergence of adults. The results of this study, although preliminary, but valuable to the field of forensic entomology because they provide new information about both ascending and descending vertical dispersal behavior of forensically important species of blowfly. Read More

View Article and Full-Text PDF

Analysis of the correlation between students' self-compassion and external pressure under chronic stress response.

Work 2021 Jun 6. Epub 2021 Jun 6.

Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo, Japan.

Background: Although the environment of college students is relatively safe, they are faced with the pressure of study, interpersonal relationship, and even future employment, which leads to a variety of psychological disorders, among which stress response is the most common one. As a new concept of healthy self, it is not clear how self - compassion affects students' stress response.

Objective: This study aimed to analyze the role of students' self-compassion based on chronic stress response in negative emotional regulation and coping style selection caused by external pressure, and to provide a theoretical basis for the application of self-compassion in regulating individual emotions in the future. Read More

View Article and Full-Text PDF

Whole exome sequencing reveals a novel LRBA mutation and clonal hematopoiesis in a common variable immunodeficiency patient presented with hemophagocytic lymphohistiocytosis.

Exp Hematol Oncol 2021 Jun 13;10(1):38. Epub 2021 Jun 13.

Myelodysplastic Syndromes Diagnosis and Therapy Center, Department of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79# Qingchun Road, Hangzhou, 310003, Zhejiang, China.

Common variable immunodeficiency (CVID) was a kind of primary immunodeficiency disorders with heterogeneous phenotype and genotype. Lipopolysaccharide-responsive and beige-like anchor (LRBA) mutation was identified as disease associated in CVID, advanced genetic method will help to detect atypical cases. We report a case of adult patient manifested as hemophagocytic lymphohistiocytosis (HLH), bone marrow examination suggested prosperity to MDS, manifested as increased immature myeloid cells and dysplastic hematopoiesis. Read More

View Article and Full-Text PDF

Quantum computing's potential for drug discovery: Early stage industry dynamics.

Drug Discov Today 2021 Jun 11. Epub 2021 Jun 11.

Faculty of Health, School of Medicine, Witten/Herdecke University, Germany. Electronic address:

Quantum computing (QC) is expected to revolutionize the drug research by performing tasks classical that supercomputers are not capable of. However, practically useful quantum computation is not yet a reality, and thus it is still unclear when and whether QC will be fully capable of solving real-world issues in drug discovery. By identifying the QC-related activities of pharmaceutical companies, startups, and academia in the field of drug discovery and development, we show that QC has gained traction across all of these stakeholder groups, that there is focus on developing utilities related to lead optimization and compound screening, and that there is a need for collaboration in the highly dynamic QC ecosystem. Read More

View Article and Full-Text PDF

Human induced pluripotent stem cell derived hepatocytes provide insights on parenteral nutrition associated cholestasis in the immature liver.

Sci Rep 2021 Jun 11;11(1):12386. Epub 2021 Jun 11.

Department of Pediatrics, Medical College of Wisconsin, 999 N. 92 Street, PO Box 1997, Milwaukee, WI, 53226, USA.

Parenteral nutrition-associated cholestasis (PNAC) significantly limits the safety of intravenous parenteral nutrition (PN). Critically ill infants are highly vulnerable to PNAC-related morbidity and mortality, however the impact of hepatic immaturity on PNAC is poorly understood. We examined developmental differences between fetal/infant and adult livers, and used human induced pluripotent stem cell-derived hepatocyte-like cells (iHLC) to gain insights into the contribution of development to altered sterol metabolism and PNAC. Read More

View Article and Full-Text PDF

Human Cord Blood B Cells Differ from the Adult Counterpart by Conserved Ig Repertoires and Accelerated Response Dynamics.

J Immunol 2021 Jun 11. Epub 2021 Jun 11.

Institute of Cell Biology (Cancer Research), Medical Faculty, University of Duisburg-Essen, Essen, Germany;

Neonatal and infant immune responses are characterized by a limited capability to generate protective Ab titers and memory B cells as seen in adults. Multiple studies support an immature or even impaired character of umbilical cord blood (UCB) B cells themselves. In this study, we provide a comprehensive molecular and functional comparison of B cell subsets from UCB and adult peripheral blood. Read More

View Article and Full-Text PDF

Conjugated linoleic acid improves meiotic spindle morphology and developmental competence of heat-stressed bovine oocyte.

Theriogenology 2021 May 27;172:67-72. Epub 2021 May 27.

Pasargad Institute for Advanced Innovative Solutions (PIAIS), Khatam University, Tehran 1991633356, Iran.

This study was conducted to elucidate the effects of introducing conjugated linoleic acid (CLA) on meiotic spindle organization of heat stressed (HS) matured oocytes and the resulting blastocysts DNA methylation as well as the expression of the genes involved in DNA methylation (DNMT3a, DNMT3b and DNMT1). Immature bovine oocytes were cultured at 41 °C for the first 12 h and 38.5 °C for the second 12 h of maturation time in the presence of 0 and 50 μM of CLA (HS and HS + CLA groups, respectively). Read More

View Article and Full-Text PDF

Lrig1 deficiency affects hippocampal dendrite complexity and impairs cognitive function.

Dev Neurobiol 2021 Jun 10. Epub 2021 Jun 10.

Instituto de Biología Celular y Neurociencias "Prof. E. De Robertis"(IBCN)- CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.

Leucine-rich repeat (LRR) transmembrane proteins have been directly linked to neurodevelopmental and cognitive disorders. We have previously shown that the LRR transmembrane protein Lrig1 is a physiological regulator of dendrite complexity of hippocampal pyramidal neurons and social behavior. In this study, we performed a battery of behavioral tests to evaluate spatial memory and cognitive capabilities in Lrig1 mutant mice. Read More

View Article and Full-Text PDF

A Concept Analysis on the Use of Artificial Intelligence in Nursing.

Zhida Shang

Cureus 2021 May 5;13(5):e14857. Epub 2021 May 5.

Faculty of Medicine and Health Sciences, McGill University, Montreal, CAN.

Artificial intelligence (AI) has a considerable present and future influence on healthcare. Nurses, representing the largest proportion of healthcare workers, are set to immensely benefit from this technology. However, the overall adoption of new technologies by nurses is quite slow, and the use of AI in nursing is considered to be in its infancy. Read More

View Article and Full-Text PDF

Primary mediastinal germ cell tumours with high prevalence of somatic malignancy: An experience from a single tertiary care oncology centre.

Ann Diagn Pathol 2021 May 26;53:151763. Epub 2021 May 26.

Department of Thoracic Surgery, Tata Memorial Hospital, Homi Bhabha National Institute, Mumbai, Maharashtra, India.

Background: Primary mediastinal germ tumours (PMGCT) constitute, a mere 3-4% of all germ cell tumours (GCT). Although they account for approximately 16% of mediastinal tumours in adults and 19-25% in children as per western literature, there is hardly any large series on PMGCT reported from the Indian subcontinent.

Design: We have retrospectively analysed clinicopathological features of 98 cases of PMGCT diagnosed over 10 years (2010-2019) from a tertiary-care oncology centre. Read More

View Article and Full-Text PDF

The effects of short time hyperoxia on glutamate concentration and glutamate transporters expressions in brain of neonatal rats.

Neurosci Lett 2021 Jul 7;758:136013. Epub 2021 Jun 7.

Neonatology Department, Anhui Provincial Children's Hospital, Hefei, China.

Preterm infants often suffer from impaired postnatal brain development, and glutamate excitotoxicity is identified as a pivotal mechanism of hyperoxia-induced neurological abnormality. We aimed to investigate the effect of short time hyperoxia on glutamate homeostasis and glutamate transporters expressions in immature brain. Six-day-old (P6) rat pups were exposed to 80% oxygen for 24 h (the hyperoxia group) or placed in atmospheric air (the control group). Read More

View Article and Full-Text PDF