43 results match your criteria il4 immature

Expression of Cntn1 is regulated by stress and associated with anxiety and depression phenotypes.

Brain Behav Immun 2021 Jul 15;95:142-153. Epub 2021 Mar 15.

Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, Hunan Province 410013, China. Electronic address:

In recent years, our understanding of neural circuits associated with depression has increased. Although inherited factors are known to influence individual differences in the risk for this disorder, it has been difficult to identify specific genes that moderate circuit functions affecting depression. Genome-wide association studies have identified genetic variants of Cntn1 that are linked to major depressive disorders. Read More

View Article and Full-Text PDF

IL-1β prevents ILC2 expansion, type 2 cytokine secretion, and mucus metaplasia in response to early-life rhinovirus infection in mice.

Allergy 2020 08 10;75(8):2005-2019. Epub 2020 Mar 10.

Departments of Pediatrics, University of Michigan Medical School, Ann Arbor, Michigan.

Background: Early-life wheezing-associated respiratory infection with human rhinovirus (RV) is associated with asthma development. RV infection of 6-day-old immature mice causes mucous metaplasia and airway hyperresponsiveness which is associated with the expansion of IL-13-producing type 2 innate lymphoid cells (ILC2s) and dependent on IL-25 and IL-33. We examined regulation of this asthma-like phenotype by IL-1β. Read More

View Article and Full-Text PDF

IFN-DC Loaded with Autophagosomes containing Virus Antigen is Highly Efficient in Inducing Virus-Specific Human T Cells.

Int J Med Sci 2019 10;16(5):741-750. Epub 2019 May 10.

Clinical Research Center, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine. Zhong Fu Road, Gulou District, Nanjing, Jiangsu, PR China 210003.

Autophagy plays a critical role in the regulation of innate and adaptive immune responses to pathogens and tumors. A previous study utilized proteasome and lysosome inhibitors to form autophagosomes (DRibbles) and the effect of dendritic cells (DCs) loaded with DRibbles in activating antigen-specific T cells has been demonstrated in a mouse experiment and human IL-4-DC. In this study, CMV-DRibbles derived from MDA cell lines expressing cytomegalovirus (CMV) pp65 protein were loaded onto human IFN-DC and IL-4-DC derived from monocytes, respectively. Read More

View Article and Full-Text PDF
December 2019

Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals.

Hum Vaccin Immunother 2019 20;15(9):2140-2149. Epub 2018 Dec 20.

Laboratório de Dermatologia e Imunodeficiências LIM56, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo , Sao Paulo , SP , Brazil.

Dendritic cell (DC)-based immunotherapy is a promising strategy for the treatment of HIV-infected individuals. Different from the conventional protocol for DC differentiation based on the cytokine IL-4 (IL4-DCs), several studies have suggested obtaining DCs by culturing monocytes with type I IFN (IFN-α) to yield IFN-DCs, as performed in cancer therapy. To evaluate the phenotypic and functional characteristics, monocytes from HIV-infected subjects were differentiated into IFN-DCs or IL4-DCs, pulsed with chemically inactivated HIV and stimulated with pro-inflammatory cytokines. Read More

View Article and Full-Text PDF

The oligosaccharides 6'-sialyllactose, 2'-fucosyllactose or galactooligosaccharides do not directly modulate human dendritic cell differentiation or maturation.

PLoS One 2018 10;13(7):e0200356. Epub 2018 Jul 10.

Cell Biology and Immunology group, Wageningen University, Wageningen, the Netherlands.

Breast milk plays an important role in immune development in early life and protects against diseases later in life. A wide range of the beneficial effects of breast milk are attributed to human milk oligosaccharides (HMOs) as well as components such as vitamin D3 (VitD3) or TGFβ. One mechanism by which HMOs might contribute to immune homeostasis and protection against disease is the induction of a local tolerogenic milieu. Read More

View Article and Full-Text PDF
January 2019

In vitro maturation of monocyte-derived dendritic cells results in two populations of cells with different surface marker expression, independently of applied concentration of interleukin-4.

Int Immunopharmacol 2018 Apr 28;57:165-171. Epub 2018 Feb 28.

Jagiellonian University Medical College, Faculty of Medicine, Department of Pharmacology, Ul. Grzegórzecka 16, 31-531 Kraków, Poland.

Dendritic cells (DCs) play a crucial role in the development of adaptive immune response. Monocyte-derived dendritic cells (MDDCs) are generated in vitro to study DC biology and for use in immunotherapy. However, procedures to generate MDDCs vary and an impact this may have on their final phenotype is insufficiently studied. Read More

View Article and Full-Text PDF

Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC.

Mol Med Rep 2017 Nov 21;16(5):7345-7354. Epub 2017 Sep 21.

Cancer Research and Biotherapy Center, The Second Hospital of Nanjing, Medical School, Southeast University, Nanjing, Jiangsu 210003, P.R. China.

Dendritic cells (DCs) as professional antigen presenting cells, are important in the initiation of the primary immune response. The present study compared the morphology, phenotypes and function between monocyte‑derived human DCs produced from a conventional culturing system containing granulocyte‑macrophage colony‑stimulating factor (GM‑CSF) and IL‑4 (IL‑4‑DC) and DCs generated by the stimulation of GM‑CSF and interferon (IFN)‑α (IFN‑DC). When compared with IL‑4‑DC in morphology, IFN‑DC contained more organelles, including endoplasmic reticulum and myelin figures, whereas mature (m)IL‑4‑DC contained more vacuoles in the cells. Read More

View Article and Full-Text PDF
November 2017

Interleukin 4 induces apoptosis of acute myeloid leukemia cells in a Stat6-dependent manner.

Leukemia 2018 03 18;32(3):588-596. Epub 2017 Aug 18.

Department of Clinical Genetics, Lund University, Lund, Sweden.

Cytokines provide signals that regulate immature normal and acute myeloid leukemia (AML) cells in the bone marrow microenvironment. We here identify interleukin 4 (IL4) as a selective inhibitor of AML cell growth and survival in a cytokine screen using fluorescently labeled AML cells. RNA-sequencing of the AML cells revealed an IL4-induced upregulation of Stat6 target genes and enrichment of apoptosis-related gene expression signatures. Read More

View Article and Full-Text PDF

Immunological Analysis and Generation of Dendritic Cells From Lavage Fluid Obtained by Cell-Free and Concentrated Ascites Reinfusion Therapy.

Ther Apher Dial 2017 Jun;21(3):255-262

Department of Palliative Medicine, Sapporo Kousei Hospital, Sapporo, Japan.

Cell-free and concentrated ascites reinfusion therapy (CART) is an effective treatment for patients with refractory ascites. Cellular components such as cancer cells and blood cells are removed and discarded. The aim of this study was to investigate the alteration of immune cells in lavage fluid and the generation of dendritic cells (DCs) from lavage fluid obtained by CART. Read More

View Article and Full-Text PDF

[Immature dendritic cells phagocytosing human spleen cells treated by PUVA present the characteristics of regulatory dendritic cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2017 Apr;33(4):455-459

75559 Unit Hospital, Wuzhishan 572200, China.

Objective To investigate the effect of psoralen combined with A-band ultraviolet (PUVA)-treated human spleen lymphocytes on the phenotype and function of immature dendritic cells (imDCs). Methods Human peripheral blood mononuclear cells (PBMCs) were isolated and induced to produce DCs by interleukin-4 (IL-4) and recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF). On the sixth day, the imDCs were obtained and stimulated by lipopolysaccharide (LPS). Read More

View Article and Full-Text PDF

[Experimental study on the immune response of fusion tumor vaccine of HepG2 and dendritic cells in vitro].

Zhonghua Yi Xue Za Zhi 2017 Feb;97(7):535-539

Gynecological Oncology Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.

To estimate the immune response of HepG2/dendritic cell (DC) fusion cells vaccines against HepG2 cells in vitro. Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density-gradient centrifugation.Then DC were obtain from PBMCs by culturing in medium containing granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 5 days. Read More

View Article and Full-Text PDF
February 2017

Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47.

Sci Rep 2017 02 27;7:43441. Epub 2017 Feb 27.

Laboratory of Human Retrovirology and Immunoinformatics, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, USA.

Interleukin (IL)-27, a member of the IL-12 cytokine family, plays an important and diverse role in the function of the immune system. We have previously demonstrated that IL-27 is an anti-viral cytokine which inhibits HIV-1, HIV-2, Influenza virus and herpes simplex virus infection, and enhances the potential of reactive oxygen species (ROS) generating activity during differentiation of monocytes to macrophages. In this study, we further investigated the mechanism of the enhanced potential for ROS generation by IL-27. Read More

View Article and Full-Text PDF
February 2017

CHI3L1 nuclear localization in monocyte derived dendritic cells.

Immunobiology 2016 Feb 9;221(2):347-56. Epub 2015 Oct 9.

Department of Biomedical and Biotechnology Sciences, University of Catania, Italy.

Chitinase-3-like-1 protein (CHI3L1) is a glycosyl hydrolase (GH) highly expressed in a variety of inflammatory diseases at infectious and non-infectious etiology. CHI3L1 is produced by a wide variety of cells including monocyte-derived macrophages cell lines such as polarized M1 and M2 type macrophages, osteoclasts and Kupffer cells. In this study we have examined the expression of CHI3L1 during the differentiation and maturation of dendritic cells. Read More

View Article and Full-Text PDF
February 2016

Vascular endothelial growth factor affects dendritic cell activity in hypertensive disorders of pregnancy.

Mol Med Rep 2015 Sep 14;12(3):3781-3786. Epub 2015 May 14.

Department of Laboratory Medicine, Nantong Women and Children Health Care Hospital, Nantong, Jiangsu 226018, P.R. China.

Vascular endothelial growth factor (VEGF) activity is involved in the growth and stability of the placenta, and its signaling has been implicated in the development of pregnancy‑induced hypertension (PIH). The present study sought to evaluate VEGF levels and dendritic cell (DC) profiles in patients with PIH, and to investigate the effects of VEGF expression on DC phenotypes. The present study assessed 162 patients, 112 of whom were diagnosed with PIH. Read More

View Article and Full-Text PDF
September 2015

Generation of mouse pluripotent stem cell-derived proliferating myeloid cells as an unlimited source of functional antigen-presenting cells.

Cancer Immunol Res 2015 Jun 11;3(6):668-77. Epub 2015 Feb 11.

Division of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan. CREST, Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.

The use of dendritic cells (DC) to prime tumor-associated antigen-specific T-cell responses provides a promising approach to cancer immunotherapy. Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) can differentiate into functional DCs, thus providing an unlimited source of DCs. However, the previously established methods of generating practical volumes of DCs from pluripotent stem cells (PSC) require a large number of PSCs at the start of the differentiation culture. Read More

View Article and Full-Text PDF

The influence of Ouabain on human dendritic cells maturation.

Mediators Inflamm 2014 28;2014:494956. Epub 2014 Dec 28.

Laboratorio de Imunologia Tumoral, Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciencias da Saude, Bloco H02, Sala 003, Universidade Federal do Rio de Janeiro, 21 941-902 Rio de Janeiro, RJ, Brazil.

Although known as a Na,K-ATPase inhibitor, several other cellular and systemic actions have been ascribed to the steroid Ouabain (Oua). Particularly in the immune system, our group showed that Ouabain acts on decreasing lymphocyte proliferation, synergizing with glucocorticoids in spontaneous thymocyte apoptosis, and also lessening CD14 expression and blocking CD16 upregulation on human monocytes. However, Ouabain effects on dendritic cells (DCs) were not explored so far. Read More

View Article and Full-Text PDF
September 2015

CCL18 promotes epithelial-mesenchymal transition, invasion and migration of pancreatic cancer cells in pancreatic ductal adenocarcinoma.

Int J Oncol 2015 Mar 10;46(3):1109-20. Epub 2014 Dec 10.

Department of General Surgery, Pancreatic Surgery, The First Affiliated Hospital of China Medical University, Heping District, Shenyang 110001, P.R. China.

CCL18 is a chemokine that is primarily expressed in monocytes, macrophages and immature dendritic cells and plays a crucial role in immune and inflammation responses. Recently, CCL18 was found to play pivotal roles in the development of several kinds of cancers, but its expression status and role during the tumorigenesis of pancreatic cancer remain unknown. In this study, we performed immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) to evaluate the expression of CCL18 in human pancreatic ductal adenocarcinoma (PDAC) tissues and preoperative serum, respectively. Read More

View Article and Full-Text PDF

Diagnosis and evaluation of severity of sepsis via the use of biomarkers and profiles of 13 cytokines: a multiplex analysis.

Clin Chem Lab Med 2015 Mar;53(4):575-81

Background: The object of this study was to evaluate biomarkers for diagnosis of sepsis, hematologic parameters, and cytokine profiles for use in the diagnosis and evaluation of severity of sepsis.

Methods: We enrolled 127 consecutive patients with systemic inflammatory response syndrome (SIRS), 97 of whom were diagnosed with sepsis. The following biomarkers were evaluated: procalcitonin (PCT); C-reactive protein (CRP); erythrocyte sedimentation rate (ESR); white blood cell count, immature granulocyte (IG) count; and multiplex cytokines, including interleukin (IL)1-β (IL1β), IL2, IL4, IL5, IL6, IL9, IL10, IL12p70, IL13, IL17, IL22, tumor necrosis factor-α (TNFα), and interferon-γ (IFNγ). Read More

View Article and Full-Text PDF

Capsaicin-mediated apoptosis of human bladder cancer cells activates dendritic cells via CD91.

Nutrition 2015 Apr 29;31(4):578-81. Epub 2014 May 29.

Department of Ecological and Biological Sciences, La Tuscia University, Viterbo, Italy. Electronic address:

Objectives: Immunostimulation by anticancer cytotoxic drugs is needed for long-term therapeutic success. Activation of dendritic cells (DCs) is crucial to obtain effective and long-lasting anticancer T-cell mediated immunity. The aim of this study was to explore the effect of capsaicin-mediated cell death of bladder cancer cells on the activation of human monocyte-derived CD1a+ immature DCs. Read More

View Article and Full-Text PDF

Induction of suppressive phenotype in monocyte-derived dendritic cells by leukemic cell products and IL-1β.

Hum Immunol 2014 Jul 24;75(7):641-9. Epub 2014 Apr 24.

Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:

Professional antigen-presenting cells, dendritic cells (DCs) play an important role in controlling tumors. It is known that solid tumor cell products inhibit DC differentiation. Recently a similar effect produced by leukemic cell products has been demonstrated. Read More

View Article and Full-Text PDF

Leukoreduction system chambers are an efficient, valid, and economic source of functional monocyte-derived dendritic cells and lymphocytes.

Immunobiology 2013 Nov 25;218(11):1392-401. Epub 2013 Jul 25.

Department of Dermatology, Universitätsklinikum Erlangen, Hartmannstraße 14, 91052 Erlangen, Germany.

The demand for human monocyte-derived dendritic cells (moDCs), as well as for primary human B and T lymphocytes for immunological research purposes has been increased in recent years. Classically, these monocytes are isolated from blood, leukapheresis products or buffy coats of healthy donors by plastic adherence of peripheral blood mononuclear cells (PBMCs), followed by stimulation with granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4, while lymphocytes are usually isolated from the non-adherent fraction (NAF) by magnetic cell sorting. However, donor-blood is a limited resource and not every blood bank offers leukapheresis products or buffy coats for laboratory use. Read More

View Article and Full-Text PDF
November 2013

In vitro induction of potent tumor-specific cytotoxic T lymphocytes using TLR agonist-activated AML-DC.

Target Oncol 2014 Sep 14;9(3):225-37. Epub 2013 Jul 14.

Immunology Department, School of Medicine, Tehran University of Medical Sciences, Poorsina Ave., 16 Azar St., Keshavarz Blvd., P.O. Box: 14155-6447, Tehran, Islamic Republic of Iran.

Dendritic cells (DCs) are recognized as key regulators of the immune system. Active DC immunization protocols are quickly obtaining interest as an alternative therapeutic approach in acute myeloid leukemia patients. Despite apparent progress in DC-based immunotherapy, some discrepancies were reported in generating potent DCs and their source. Read More

View Article and Full-Text PDF
September 2014

Immunomodulatory effects of human umbilical cord Wharton's jelly-derived mesenchymal stem cells on differentiation, maturation and endocytosis of monocyte-derived dendritic cells.

Iran J Allergy Asthma Immunol 2013 Mar;12(1):37-49

Molecular Immunology Research Center and Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran.

The Wharton's jelly of the umbilical cord is believed to be a source of mesenchymal stem cells (MSCs) which can be therapeutically applied in degenerative diseases.In this study, we investigated the immunomodulatory effect of umbilical cord derived-mesenchymal stem cells (UC-MSCs) and bone marrow-derived-mesenchymal stem cells (BM-MSCs) on differentiation, maturation, and endocytosis of monocyte-derived dendritic cells in a transwell culture system under laboratory conditions. Monocytes were differentiated into immature dendritic cells (iDCs) in the presence of GM-CSF and IL-4 for 6 days and then differentiated into mature dendritic cells (mDCs) in the presence of TNF-α for 2 days. Read More

View Article and Full-Text PDF

GM-CSF and IL-4 stimulate antibody responses in humanized mice by promoting T, B, and dendritic cell maturation.

J Immunol 2012 Dec 22;189(11):5223-9. Epub 2012 Oct 22.

Interdisciplinary Research Group in Infectious Diseases, Singapore-MIT Alliance for Research and Technology, Singapore 138602.

Engraftment of human hematopoietic stem cells into immunodeficient mice that lack T cells, B cells, and NK cells results in reconstitution of human blood lineage cells, especially B cells, in the recipient mice. However, these humanized mice do not make any significant level of IgG Ab in response to Ag stimulation. In this study, we show that in humanized mice, B cells are immature, and there is a complete deficiency of CD209(+) (DC-SIGN) human dendritic cells. Read More

View Article and Full-Text PDF
December 2012

Prolonged expression of MHC class I - peptide expression in bone marrow derived retrovirus transfected matured dendritic cells by continuous centrifugation in the presence of IL-4.

L M Hettihewa

Indian J Med Res 2011 Nov;134(5):672-8

Department of Pharmacology, Faculty of Medicine, University of Ruhuna, Sri Lanka.

Background & Objectives: Dendritic cells (DCs) are potent antigen presenting cells which proceed from immature to a mature stage during their differentiation. There are several methods of obtaining long lasting mature antigen expressing DCs and different methods show different levels of antigen expressions. We investigated bone marrow derived DCs for the degree of maturation and genetically engineered antigen presentation in the presence of interleukin-4 (IL-4) as a maturity enhancer. Read More

View Article and Full-Text PDF
November 2011

Intravenous immunoglobulin modulates the maturation of TLR 4-primed peripheral blood monocytes.

Clin Immunol 2011 May 15;139(2):208-14. Epub 2011 Mar 15.

Allergy/Clinical Immunology Division, Women and Children's Hospital, 219 Bryant St, Buffalo, NY 14222, USA.

Intravenous immunoglobulin (IgIV) has immune modulating effects on the differentiation and function of dendritic cells (DC). Peripheral blood CD14+ monocytes were induced to differentiate into immature DC with IL-4/GM-CSF. DC maturation was analyzed by flow cytometry, and function assessed for antigen uptake and antigen processing. Read More

View Article and Full-Text PDF

The impact of maternal HIV infection on cord blood lymphocyte subsets and cytokine profile in exposed non-infected newborns.

BMC Infect Dis 2011 Feb 3;11:38. Epub 2011 Feb 3.

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas, Rua Tessalia Vieira de Camargo 126, 13083-887 - Campinas, Brazil.

Background: Children born to HIV+ mothers are exposed intra-utero to several drugs and cytokines that can modify the developing immune system, and influence the newborn's immune response to infections and vaccines. We analyzed the relation between the distribution of cord blood lymphocyte subsets and cytokine profile in term newborns of HIV+ mothers using HAART during pregnancy and compared them to normal newborns.

Methods: In a prospective, controlled study, 36 mother-child pairs from HIV+ mothers and 15 HIV-uninfected mothers were studied. Read More

View Article and Full-Text PDF
February 2011

Regulatory T cells in many flavors control asthma.

Mucosal Immunol 2010 May 17;3(3):216-29. Epub 2010 Feb 17.

Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

That regulatory T cells (Tregs) have a crucial role in controlling allergic diseases such as asthma is now undisputed. The cytokines most commonly implicated in Treg-mediated suppression of allergic asthma are transforming growth factor-beta (TGF-beta) and interleukin (IL)-10). In addition to naturally occurring Tregs, adaptive Tregs, induced in response to foreign antigens, have been shown in recent studies. Read More

View Article and Full-Text PDF

Regulation of expression and secretion of galectin-3 in human monocyte-derived dendritic cells.

Mol Immunol 2009 Oct 21;46(16):3292-9. Epub 2009 Aug 21.

Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands.

Galectin-3 (Gal-3) is a beta-galactoside binding lectin displaying both intracellular and extracellular immune functions. In Schistosoma mansoni infection, Gal-3 has been associated with the induction of a T helper 2 response. Whereas dendritic cells (DCs) play a pivotal role in the regulation of T cell differentiation, little is known about the regulation of Gal-3 expression in DCs. Read More

View Article and Full-Text PDF
October 2009

Cryopreservation of monocytes is superior to cryopreservation of immature or semi-mature dendritic cells for dendritic cell-based immunotherapy.

J Immunother 2009 Jul-Aug;32(6):638-54

Department of Surgery, Medical University of Vienna, Vienna, Austria.

Cryopreservation of immature or mature dendritic cells (DC) has been proposed as a suitable method to gain large numbers of DC for immunotherapeutic trials against cancer. However, clinical studies using cryopreserved DC have demonstrated only limited success so far. The aim of this study was to investigate whether cryopreservation of monocytes elicits more potent DC and whether these DC are comparable to freshly generated DC preparations. Read More

View Article and Full-Text PDF
October 2009