7,121 results match your criteria igg testing

Electrochemiluminescence Immunoassay Platform with Immunoglobulin G-Encapsulated Gold Nanoclusters as a "Two-In-One" Probe.

Anal Chem 2021 Sep 15. Epub 2021 Sep 15.

Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China.

Biomolecule-functionalized Au nanoclusters (AuNCs) have drawn great interest in the electrochemiluminescence (ECL) field due to their unique optical/electrical properties, biocompatibility, and versatile bioapplication potentials. Herein, we proposed a two-in-one ECL probe of immunoglobulin G-encapsulated AuNCs (IgG-AuNCs) for the development of a high-performance ECL immunoassay (ECLIA) platform. The IgG-AuNCs were not only used as an ECL probe due to their excellent anodic ECL performance with triethylamine (TEA) as a coreactant but also used as the biorecognition element because of their well-retained bioactivity of the IgG. Read More

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September 2021

A Comparison of the Performance of SARS-CoV-2 Antibody Assays in Healthcare Workers with COVID-19.

Ir Med J 2021 Aug 19;114(7):414. Epub 2021 Aug 19.

Genitourinary Medicine and Infectious Diseases Department (GUIDe), St. James's Hospital, Dublin.

Aims Since its emergence, significant interest surrounds the use of SARS-CoV-2 serological tests as an alternative or as an adjunct to molecular testing. However, given the speed of this pandemic, paralleled with the pressure to develop and provide serological tests in an expediated manner, not every assay has undergone the rigorous evaluation that is usually associated with medical diagnostic assays. We aimed to examine the performance of several commercially available SARS-CoV-2 IgG antibody assays among participants with confirmed COVID-19 disease and negative controls. Read More

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Optimal strategies to screen health care workers for COVID-19 in the US: a cost-effectiveness analysis.

Res Sq 2021 Sep 10. Epub 2021 Sep 10.

Background: Transmission of SARS-CoV-2 in health care facilities poses a challenge against pandemic control. Health care workers (HCWs) have frequent and high-risk interactions with COVID-19 patients. We undertook a cost-effectiveness analysis to determine optimal testing strategies for screening HCWs to inform strategic decision-making in health care settings. Read More

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September 2021

MALDI-ToF Protein Profiling as a Potential Rapid Diagnostic Platform for COVID-19.

J Mass Spectrom Adv Clin Lab 2021 Sep 9. Epub 2021 Sep 9.

Departments of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115, United States.

More than a year after the COVID-19 pandemic was declared, the need still exists for accurate, rapid, inexpensive and non-invasive diagnostic methods that yield high specificity and sensitivity towards the current and newly emerging SARS-CoV-2 strains. Compared to the nasopharyngeal (NP) swabs, several studies have established saliva as a more amenable specimen type for early detection of SARS-CoV-2. Considering the limitations and high demand for COVID-19 testing, we employed MALDI-ToF mass spectrometry in the analysis of 60 gargle samples from human donors and compared the resultant spectra against COVID-19 status. Read More

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September 2021

Rapid and quantitative detection of SARS-CoV-2 IgG antibody in serum using optofluidic point-of-care testing fluorescence biosensor.

Talanta 2021 Dec 14;235:122800. Epub 2021 Aug 14.

School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China. Electronic address:

The COVID-19 pandemic brings unprecedented crisis for public health and economics in the world. Detecting specific antibodies to SARS-CoV-2 is a powerful supplement for the diagnosis of COVID-19 and is important for epidemiological studies and vaccine validations. Herein, a rapid and quantitative detection method of anti-SARS-CoV-2 IgG antibody was built based on the optofluidic point-of-care testing fluorescence biosensor. Read More

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December 2021

Rapid and Laboratory SARS-CoV-2 Antibody Testing in High-Risk Hospital Associated Cohorts of Unknown COVID-19 Exposure, a Validation and Epidemiological Study After the First Wave of the Pandemic.

Front Med (Lausanne) 2021 26;8:642318. Epub 2021 Aug 26.

Infectious Diseases Department, Mater Misericordiae University Hospital, Dublin, Ireland.

We aimed to use SARS-CoV-2 antibody tests to assess the asymptomatic seroprevalence of individuals in high-risk hospital cohorts who's previous COVID-19 exposure is unknown; staff, and patients requiring haemodialysis or chemotherapy after the first wave. In a single Center, study participants had five SARS-CoV-2 antibody tests done simultaneously; one rapid diagnostic test (RDT) (Superbio Colloidal Gold IgM/IgG), and four laboratory tests (Roche Elecsys® Anti-SARS-CoV-2 IgG [RE], Abbott Architect i2000SR IgG [AAr], Abbott Alinity IgG [AAl], and Abbott Architect IgM CMIA). To determine seroprevalence, only positive test results on laboratory assay were considered true positives. Read More

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Seroprevalence of Cytomegalovirus and Associated Factors Among Preconception Women: A Cross-Sectional Nationwide Study in China.

Front Public Health 2021 25;9:631411. Epub 2021 Aug 25.

Women's Health and Perinatology Research Group, Department of Clinical Medicine, UiT - The Arctic University of Norway, Tromsø, Norway.

Cytomegalovirus seroconversion during pregnancy is common and has a substantial risk of congenital infection with longterm sequale. Screening during pregnancy or vaccination have not been shown to be effective for eliminating congenital infections. Preconception screening policy has not been evaluated adequately in a large scale. Read More

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September 2021

Migration in times of pandemic: SARS-CoV-2 infection among the Warao indigenous refugees in Belém, Pará, Amazonia, Brazil.

BMC Public Health 2021 09 13;21(1):1659. Epub 2021 Sep 13.

Secretaria de Saúde do Município de Belém (SESMA), Belem, Brazil.

Background: The emergence of the new causative agent of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in the city of Wuhan, China, in December 2019, and its spread worldwide, led the World Health Organization (WHO) to declare a pandemic. The disease has caused high mortality among traditional populations and the most socially vulnerable groups such indigenous and refugees. The present study aims to investigate the prevalence of anti-SARS-CoV-2 IgG antibodies in the population of Venezuelan indigenous Warao refugees residing in private and public shelters in the city of Belem, capital of Para State, in the Brazilian Amazon. Read More

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September 2021

Dynamic IgG seropositivity after rollout of CoronaVac and BNT162b2 COVID-19 vaccines in Chile: a sentinel surveillance study.

Lancet Infect Dis 2021 Sep 9. Epub 2021 Sep 9.

Departamento de Ingeniería Civil, Universidad de Chile, Santiago, Chile; Instituto Sistemas Complejos de Ingeniería, Santiago, Chile. Electronic address:

Background: By July 14, 2021, 81·3 % of adults (aged ≥18 years) in Chile had received a first SARS-CoV-2 vaccine and 72·3% had received a second SARS-CoV-2 vaccine, with the majority of people given Sinovac's inactivated CoronaVac vaccine (75·3% of vaccines dispensed) or Pfizer-BioNTech's mRNA BNT162b2 vaccine (20·9% of vaccines dispensed). Due to the absence of simultaneous real-world data for these vaccines, we aimed to compare SARS-CoV-2 IgG positivity between vaccines using a dynamic national monitoring strategy.

Methods: From March 12, 2021, 28 testing stations for SARS-CoV-2 IgG detection were installed in hotspots based on cellular-phone mobility tracking within the most populated cities in Chile. Read More

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September 2021

SARS-CoV-2 Antibody responses in solid organ transplant recipients.

Transpl Infect Dis 2021 Sep 10. Epub 2021 Sep 10.

New York University Grossman School of Medicine, New York, NY, USA.

Antibody responses among immunocompromised solid organ transplant recipients (SOT) infected with SARS-CoV-2 may be diminished compared to the general population and have not been fully characterized. We conducted a cohort study at our transplant center to investigate the rate of seroconversion for SARS-CoV-2 IgG antibodies among SOT recipients who were diagnosed with COVID-19 and underwent serum SARS-CoV-2 IgG ELISA testing. The 61 patients who were included in the final analysis underwent initial SARS-CoV-2 IgG testing at a median of 62 days (IQR 55. Read More

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September 2021

Low peripheral mitochondrial DNA copy number during manic episodes of bipolar disorders is associated with disease severity and inflammation.

Brain Behav Immun 2021 Sep 6;98:349-356. Epub 2021 Sep 6.

Univ Paris Est Creteil, INSERM, IMRB Translational Neuropsychiatry laboratory, AP-HP, DMU IMPACT & FHU ADAPT, Fondation FondaMental, F-94010, Creteil, France. Electronic address:

Mitochondria (Mt) are intra-cellular components essential for cellular energy processes whose dysfunction may induce premature cellular senescence and/or inflammation, both observed in bipolar disorders (BD). We investigated mitochondrial DNA copy number (mtDNAcn) levels in patients with BD being in manic, depressive or euthymic phase and in healthy controls (HC) both characterized for the levels of blood-based inflammatory markers and stigma of pathogens. 312 patients with BD were compared to 180 HC. Read More

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September 2021

Optimizing use of multi-antibody assays for Lyme disease diagnosis: A bioinformatic approach.

PLoS One 2021 9;16(9):e0253514. Epub 2021 Sep 9.

Clinical Research Operations & Regulatory Affairs, Lewis Katz School of Medicine, Temple University, Philadelphia, Pennsylvania, United States of America.

Multiple different recombinant and peptide antigens are now available for serodiagnosis of Lyme disease (LD), but optimizing test utilization remains challenging. Since 1995 the Centers for Disease Control and Prevention (CDC) has recommended a 2-tiered serologic approach consisting of a first-tier whole-cell enzyme immunoassay (EIA) for polyvalent antibodies to Borrelia burgdorferi followed by confirmation of positive or equivocal results by IgG and IgM immunoblots [standard 2-tiered (STT) approach]. Newer modified 2-tiered (MTT) approaches employ a second-tier EIA to detect antibodies to B. Read More

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September 2021

Evaluation of anti-SARS-CoV-2 antibody levels: two different methods.

Rev Assoc Med Bras (1992) 2021 Apr;67(4):597-601

Health Sciences University Gazi Yaşargil Training and Research Hospital, Department of Clinical Microbiology Laboratory - Kayapınar, Turkey.

Objective: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) is still a major problem worldwide. Antibody response to SARS-CoV-2 has not yet been fully clarified, and clinical benefits of serological tests remain unclear. Despite the presence of numerous systems and methods used to analyze antibody levels, it is difficult to mention about standardization. Read More

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Detection of Anti-SARS-CoV-2-S2 IgG Is More Sensitive Than Anti-RBD IgG in Identifying Asymptomatic COVID-19 Patients.

Front Immunol 2021 19;12:724763. Epub 2021 Aug 19.

Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.

Characterizing the serologic features of asymptomatic SARS-CoV-2 infection is imperative to improve diagnostics and control of SARS-CoV-2 transmission. In this study, we evaluated the antibody profiles in 272 plasma samples collected from 59 COVID-19 patients, consisting of 18 asymptomatic patients, 33 mildly ill patients and 8 severely ill patients. We measured the IgG against five viral structural proteins, different isotypes of immunoglobulins against the Receptor Binding Domain (RBD) protein, and neutralizing antibodies. Read More

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September 2021

Serological Testing for COVID-19, Immunological Surveillance, and Exploration of Protective Antibodies.

Front Immunol 2021 19;12:635701. Epub 2021 Aug 19.

Brazilian Biosciences National Laboratory, Center for Research in Energy and Materials, Campinas, Brazil.

Serological testing is a powerful tool in epidemiological studies for understanding viral circulation and assessing the effectiveness of virus control measures, as is the case of SARS-CoV-2, the pathogenic agent of COVID-19. Immunoassays can quantitatively reveal the concentration of antiviral antibodies. The assessment of antiviral antibody titers may provide information on virus exposure, and changes in IgG levels are also indicative of a reduction in viral circulation. Read More

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September 2021

Seroprevalence of Dengue and Chikungunya antibodies among blood donors in Dar es Salaam and Zanzibar, Tanzania: a cross-sectional study.

BMC Infect Dis 2021 Sep 6;21(1):911. Epub 2021 Sep 6.

Nelson Mandela African Institute of Science and Technology Arusha, Arusha, Tanzania.

Background: The potential shift of major causes of febrile illnesses from malaria to non-malarial febrile illnesses, including arboviral diseases such as chikungunya and dengue, is of concern. The last outbreaks of these infections were reported in 2018 and 2019 for chikungunya in Zanzibar and dengue in Dar es Salaam. We conducted a cross-sectional study that involved serological testing of stored blood samples from the blood banks in Temeke Referral Hospital in Dar es Salaam and the National Blood Bank Unit in Zanzibar. Read More

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September 2021

Hemodialysis patients with coronavirus disease 2019: reduced antibody response.

Clin Exp Nephrol 2021 Sep 6. Epub 2021 Sep 6.

Department of Nephrology, Tokyo Metropolitan Health and Hospitals Corporation Okubo Hospital, Tokyo, Japan.

Background: Because patients on maintenance hemodialysis (HD) have an impaired immune response to pathogens, they are at higher risk of severe coronavirus disease 2019 (COVID-19). However, data on antibody production among HD patients with COVID-19 is scarce. Thus, we performed a retrospective cohort study evaluating severe acute respiratory syndrome coronavirus two antibody (SARS-CoV-2) production within 1 month after COVID-19 onset in hospitalized patients on HD. Read More

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September 2021

Recombinant Protein Filovirus Vaccines Protect Cynomolgus Macaques From Ebola, Sudan, and Marburg Viruses.

Front Immunol 2021 18;12:703986. Epub 2021 Aug 18.

Galveston National Laboratory, Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, United States.

Ebola (EBOV), Marburg (MARV) and Sudan (SUDV) viruses are the three filoviruses which have caused the most fatalities in humans. Transmission from animals into the human population typically causes outbreaks of limited scale in endemic regions. In contrast, the 2013-16 outbreak in several West African countries claimed more than 11,000 lives revealing the true epidemic potential of filoviruses. Read More

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ABO antibody titer performance characteristics and correlates between two automated platforms.

Transfus Apher Sci 2021 Sep 1:103262. Epub 2021 Sep 1.

Department of Pathology, Stanford University School of Medicine, Stanford, CA, 94305, United States; Stanford Blood Center, Stanford Medicine, Stanford, CA, 94305, United States. Electronic address:

Background: AABB standards require a policy for assessing transfusing ABO-incompatible plasma. After a fatal hemolytic event with incompatible plasma, our institution instituted platelet donor population titer method for ABO antibodies on the PK7300, with high-titer being defined as having isohemagglutinin titers greater than 256. We recently switched titering platforms to the Neo Iris and we seek to determine the equivalent isohemagglutinin high-titer cutoff on the Neo Iris as compared to the PK7300. Read More

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September 2021

Prevalence of Celiac Disease in China: Meta-analysis and Serological Survey in High-risk Populations.

J Dig Dis 2021 Sep 5. Epub 2021 Sep 5.

Department of Gastroenterology, State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Objective: To perform a meta-analysis that investigates the prevalence and seroprevalence of celiac disease (CD) in Chinese general population and high-risk populations, and to summarize data from a serological survey of high-risk populations in Guangdong, China.

Methods: We collected data from the serological survey of high-risk populations in Guangdong, China (n = 1390) by testing serum tissue transglutaminase immunoglobulin A (tTG-IgA), deamidated gliadin peptides immunoglobulin A (DGP-IgA) and deamidated gliadin peptides immunoglobulin G (DGP-IgG), and also searched Medline, PubMed, Embase, Cochrane and 3 Chinese databases up to December 20, 2020 to estimate the pooled prevalence and seroprevalence of CD in China.

Results: In the serological survey, 0. Read More

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September 2021

Seroprevalence of SARS-CoV-2 among high-risk healthcare workers in a MERS-CoV endemic area.

J Infect Public Health 2021 Aug 25;14(9):1268-1273. Epub 2021 Aug 25.

College of Medicine, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia; King Abdullah International Medical Research Centre (KAIMRC), Ministry of National Guard Health Affairs (MNGHA), Riyadh, Saudi Arabia. Electronic address:

Introduction: Healthcare workers (HCWs) in Saudi Arabia are a unique population who have had exposures to the Middle East Respiratory Syndrome coronavirus (MERS-CoV) and Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). It follows that HCWs from this country could have pre-existingMERS-CoV antibodies that may either protect from coronavirus disease 2019 (COVID-19) infection or cause false SARS-CoV-2 seropositive results. In this article, we report the seroprevalence of MERS-CoV and SARS-CoV-2 among high-risk healthcare workers in Riyadh city, Saudi Arabia. Read More

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SARS-CoV-2-associated Guillain-Barré syndrome in four patients: what do we know about pathophysiology?

Acta Neurol Belg 2021 Sep 2. Epub 2021 Sep 2.

Department of Neurology, Cliniques de l'Europe, Avenue de Fré 206, 1180, Brussels, Belgium.

Background: A growing number of Guillain-Barré syndrome (GBS) and Miller Fisher Syndrome (MFS) cases following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are reported. Nevertheless, this association is still debated, and pathophysiology remains unclear.

Methods: Between April and December 2020, in three hospitals located in Brussels, Belgium, we examined four patients with GBS following SARS-CoV-2 infection. Read More

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September 2021

Increased vulnerability to SARS-CoV-2 infection among indigenous people living in the urban area of Manaus.

Sci Rep 2021 09 2;11(1):17534. Epub 2021 Sep 2.

Laboratório de Imunologia e Virologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação Sociedade, Ambiente e Saúde, Av. André Araújo, 2936-Petrópolis, Manaus, Amazonas, CEP 69.067-375, Brazil.

The COVID-19 pandemic threatens indigenous peoples living in suburban areas of large Brazilian cities and has thus far intensified their pre-existing socio-economic inequalities. We evaluated the epidemiological situation of SARS-CoV-2 infection among residents of the biggest urban multiethnic indigenous community of the Amazonas state, Brazil. Blood samples of 280 indigenous people living in the surrounding area of Manaus were tested for the presence of anti-SARS-CoV-2 IgA or IgG antibodies. Read More

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September 2021

Toxoplasma gondii multifocal central nervous system infiltration in an apparently immunocompetent cat in Italy.

Vet Parasitol Reg Stud Reports 2021 Jul 24;25:100605. Epub 2021 Jun 24.

Centro Veterinario Caleidos, Via Lanfranco 4, 17011 Albisola Superiore, SV, Italy.

A young cat with progressive ambulatory deficits with multifocal neurolocalization underwent spinal cord and brain magnetic resonance imaging (MRI). After serological testing through indirect immunofluorescence (IFI; IgG titer = 640, IgM titer = 160; cut-off titer for both = 80) for Toxoplasma gondii and unsuccessful 3-week therapy with clindamycin, his condition worsened. MRI revealed multiple lesions in the cervical and thoracolumbar spinal cord and brain and cerebellum meninges. Read More

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Self-collection of capillary blood using Tasso-SST devices for Anti-SARS-CoV-2 IgG antibody testing.

PLoS One 2021 2;16(9):e0255841. Epub 2021 Sep 2.

Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, United States of America.

Background: Efforts to minimize COVID-19 exposure during the current SARS-CoV-2 pandemic have led to limitations in access to medical care and testing. The Tasso-SST kit includes all of the components necessary for remote, capillary blood self-collection. In this study, we sought to investigate the accuracy and reliability of the Tasso-SST device as a self-collection device for measurement of SARS-CoV-2 IgG antibodies. Read More

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September 2021

A high-throughput microsphere-based immunoassay of anti-SARS-CoV-2 IgM testing for COVID-19 diagnostics.

PLoS One 2021 2;16(9):e0248444. Epub 2021 Sep 2.

DiaCarta Inc, Richmond, CA, United States of America.

The pandemic of novel coronavirus disease COVID-19 is rapidly expanding across the world. A positive result of antibody tests suggests that the individual has potentially been exposed to SARS-CoV-2, thus allowing to identify asymptomatic infections and determine the seroprevalence in a given population. The aim of this study was to evaluate the performances of a newly developed high throughput immunoassay for anti-SARS-CoV-2 IgM antibody detection on the Luminex MAGPIX platform. Read More

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September 2021

Evaluation of the SARS-CoV-2 Antibody Response to the BNT162b2 Vaccine in Patients Undergoing Hemodialysis.

JAMA Netw Open 2021 09 1;4(9):e2123622. Epub 2021 Sep 1.

Division of Nephrology, Department of Medicine, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.

Importance: Patients undergoing hemodialysis have a high mortality rate associated with COVID-19, and this patient population often has a poor response to vaccinations. Randomized clinical trials for COVID-19 vaccines included few patients with kidney disease; therefore, vaccine immunogenicity is uncertain in this population.

Objective: To evaluate the SARS-CoV-2 antibody response in patients undergoing chronic hemodialysis following 1 vs 2 doses of BNT162b2 COVID-19 vaccination compared with health care workers serving as controls and convalescent serum. Read More

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September 2021

Seroprevalence of SARS-CoV-2 Antibodies and Associated Factors in Health Care Workers.

J Assoc Physicians India 2021 Aug;69(8):11-12

Consultant Pathologist, Seven Hills Dedicated Covid Hospital, Mumbai, Maharashtra.

Objectives: To estimate the seroprevalence of SARS-CoV-2 antibodies among HCWs, and to study the factors associated with this seroprevalence.

Material And Methods: A cross-sectional study of HCWs from a Dedicated COVID Hospital was conducted from December 2020 to February 2021. Universal sampling for qualitative testing(by COVID-19 IgG rapid test device by Voxpress) was done and the samples which tested positive were subjected to quantitative testing (chemiluminescent immunoassay) by Serial testing. Read More

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High proportion of drug-resistant isolates in adult community-acquired pneumonia from Northeast India: A hospital-based study.

Lung India 2021 Sep-Oct;38(5):460-465

Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.

Background: Empirical antibiotic therapy is the mainstay of management of adult community-acquired pneumonia (CAP) globally. Knowledge of prevalent pathogen (bacterial) profile and drug susceptibility pattern is very essential for appropriate management of CAP cases, which again calls for regular update of pathogen profile in a given locality. This study was to identify the bacterial etiology of CAP cases and their antibiotic susceptibility pattern. Read More

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September 2021

Performance verification of the Abbott SARS-CoV-2 test for qualitative detection of IgG in Cali, Colombia.

PLoS One 2021 1;16(9):e0256566. Epub 2021 Sep 1.

Departamento de Patología y Medicina de Laboratorio, Fundación Valle del Lili, Cali, Colombia.

Background: Adequate testing is critically important for control of the SARS-CoV-2 pandemic. Antibody testing is an option for case management and epidemiologic studies, with high specificity and variable sensitivity. However, characteristics of local populations may affect performance of these tests. Read More

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September 2021