10,105 results match your criteria igg assays

Analytical and clinical performance of cPass neutralizing antibodies assay.

Clin Biochem 2021 Sep 21. Epub 2021 Sep 21.

Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX, 77030, United States; Section of Clinical Chemistry, Division of Laboratory Medicine, Department of Pathology, Texas Children's Hospital, Houston, TX, 77030, United States. Electronic address:

Serological tests for SARS-CoV-2 are a critical component of disease control strategies. SARS-CoV-2 serology tests used in clinical diagnostic should not accurately evaluate total levels the antibodies but also closely correlate with neutralizing antibodies titers. However, only limited data is available reporting correlation of neutralization antibody assays with commercial high-throughput serological assays widely used in clinical laboratories. Read More

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September 2021

Agreement between commercially available ELISA and in-house Luminex SARS-CoV-2 antibody immunoassays.

Sci Rep 2021 Sep 23;11(1):18984. Epub 2021 Sep 23.

ISGlobal, Hospital Clínic, Universitat de Barcelona, Barcelona, Catalonia, Spain.

Serological diagnostic of the severe respiratory distress syndrome coronavirus 2 (SARS-CoV-2) is a valuable tool for the determination of immunity and surveillance of exposure to the virus. In the context of an ongoing pandemic, it is essential to externally validate widely used tests to assure correct diagnostics and epidemiological estimations. We evaluated the performance of the COVID-19 ELISA IgG and the COVID-19 ELISA IgM/A (Vircell, S. Read More

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September 2021

Sero-prevalence of anti-SARS-CoV-2 antibodies among healthcare workers: A multicenter study from Egypt.

J Infect Public Health 2021 Sep 20. Epub 2021 Sep 20.

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Egypt.

Background: Healthcare workers (HCWs) are at a high risk for disease exposure. Given the limited availability of nucleic acid testing by PCR in low resource settings, serological assays can provide useful data on the proportion of HCWs who have recently or previously been infected. Therefore, in this study, we conducted an immunologic study to determine the seroprevalence of anti-SARS-CoV-2 antibodies in two university hospitals in Egypt. Read More

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September 2021

Fluorescence-encoded Poly(methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis.

Anal Biochem 2021 Sep 20:114389. Epub 2021 Sep 20.

Brandenburg Technical University Cottbus - Senftenberg, Faculty of Natural Sciences, Universitätsplatz 1, D-01968 Senftenberg, Germany. Electronic address:

Rheumatoid arthritis (RA) belongs to the most often occurring autoimmune diseases in the world. For serological diagnosis, IgM auto-antibodies directed against the Fc portion of IgG referred to as rheumatoid factor are used as biomarkers. The autoantibody detection is usually done by ELISA. Read More

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September 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence: Navigating the absence of a gold standard.

PLoS One 2021 23;16(9):e0257743. Epub 2021 Sep 23.

Microbiology Department, Canadian Blood Services, Ottawa, Canada.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence studies bridge the gap left from case detection, to estimate the true burden of the COVID-19 pandemic. While multiple anti-SARS-CoV-2 immunoassays are available, no gold standard exists.

Methods: This serial cross-sectional study was conducted using plasma samples from 8999 healthy blood donors between April-September 2020. Read More

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September 2021

A systematic evaluation of IgM and IgG antibody assay accuracy in diagnosing acute Zika Virus infection in Brazil; lessons relevant to emerging infections.

J Clin Microbiol 2021 Sep 22:JCM0289320. Epub 2021 Sep 22.

National Institute for Health Research (NIHR) Health Protection Research Unit in Emerging and Zoonotic Infections (HPRU EZI), Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.

Accurate diagnostics underpin effective public health responses to emerging viruses. For viruses, such as Zika virus (ZIKV), where the viremia clears quickly, antibody-based (IgM or IgG) diagnostics are recommended for patients who present seven days after symptom onset. However, cross-reactive antibody responses can complicate test interpretation among populations where closely related viruses circulate. Read More

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September 2021

SARS-CoV-2 Antibody Rapid Tests: Valuable Epidemiological Tools in Challenging Settings.

Microbiol Spectr 2021 Sep 22:e0025021. Epub 2021 Sep 22.

Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele, Milano, Italy.

During the last year, mass screening campaigns have been carried out to identify immunological response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and establish a possible seroprevalence. The obtained results gained new importance with the beginning of the SARS-CoV-2 vaccination campaign, as the lack of doses has persuaded several countries to introduce different policies for individuals who had a history of COVID-19. Lateral flow assays (LFAs) may represent an affordable tool to support population screening in low-middle-income countries, where diagnostic tests are lacking and epidemiology is still widely unknown. Read More

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September 2021

Correlation of sample-to-cut-off ratio of anti-SARS-CoV-2 IgG antibody chemiluminescent assay with neutralization activity: a prospective multi-centric study in India.

ISBT Sci Ser 2021 Jun 28. Epub 2021 Jun 28.

Laboratory Medicine Independent Consultant Mumbai India.

Background And Objectives: There are limited published data on association of results from commercial serological anti-SARS-CoV-2 IgG antibody CLIA (chemiluminescent immunoassay) assays with neutralizing antibodies. This study was undertaken with an objective to correlate sample-to-cut-off (S/Co) ratio of CLIA antibody tests with inhibition activity, which may then serve as a valuable guide for labelling plasma as COVID convalescent plasma (CCP) for therapy and assessing vaccine efficacy.

Materials And Methods: A total of 139 donor serum samples who were previously RT-PCR positive and had recovered completely from COVID-19 at least 28 days prior to collection of samples were recruited at three sites. Read More

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Elevated N-Linked Glycosylation of IgG V Regions in Myasthenia Gravis Disease Subtypes.

J Immunol 2021 Sep 20. Epub 2021 Sep 20.

Department of Neurology, Yale University School of Medicine, New Haven, CT;

Elevated N-linked glycosylation of IgG V regions (IgG-V) is an emerging molecular phenotype associated with autoimmune disorders. To test the broader specificity of elevated IgG-V, we studied patients with distinct subtypes of myasthenia gravis (MG), a B cell-mediated autoimmune disease. Our experimental design focused on examining the B cell repertoire and total IgG. Read More

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September 2021

Immunological assessment of SARS-CoV-2 infection in pregnancy from diagnosis to delivery: A multicentre prospective study.

PLoS One 2021 20;16(9):e0253090. Epub 2021 Sep 20.

UCD School of Medicine, National Maternity Hospital, Dublin, Ireland.

Background: Background Population-based data on SARS-CoV-2 infection in pregnancy and assessment of passive immunity to the neonate, is lacking. We profiled the maternal and fetal response using a combination of viral RNA from naso-pharyngeal swabs and serological assessment of antibodies against SARS-CoV-2.

Methods: This multicentre prospective observational study was conducted between March 24th and August 31st 2020. Read More

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September 2021

Monitoring Specific IgM and IgG Production Among Severe COVID-19 Patients Using Qualitative and Quantitative Immunodiagnostic Assays: A Retrospective Cohort Study.

Front Immunol 2021;12:705441. Epub 2021 Sep 3.

Department of Pediatrics, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.

The purpose of this study is to monitor specific anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) IgG and IgM antibody production in patients with severe forms of coronavirus disease 2019 (COVID-19) using various commercially available quantitative and qualitative tests. The sera of 23 confirmed COVID-19 patients were processed for anti-SARS-CoV-2 IgG and IgM detection. Three different immunoassays, viz. Read More

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September 2021

Effects of enzymatic hydrolysis on the allergenicity of natural cow milk based on a BALB/c mouse model.

J Dairy Sci 2021 Sep 15. Epub 2021 Sep 15.

College of Food Science, Shenyang Agricultural University, Shenyang 100866, P. R. China. Electronic address:

Cow milk allergy is one of the most prevalent food allergies worldwide, particularly in infants and children. To the best of our knowledge, minimal research exists concerning the antigenicity of cow milk (CM). This study was performed to evaluate the allergenicity of enzymatically hydrolyzed cow milk (HM) in a BALB/c mouse model. Read More

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September 2021

IgG anti-Pentraxin 3 antibodies are a novel biomarker of ANCA-associated vasculitis and better identify patients with eosinophilic granulomatosis with polyangiitis.

J Autoimmun 2021 Sep 14;124:102725. Epub 2021 Sep 14.

Division of Rheumatology, Department of Medicine DIMED, University of Padova, Italy. Electronic address:

Objective: To investigate prevalence of anti-Pentraxin 3 (PTX3) antibodies in sera of anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) patients.

Methods: Anti-PTX3 and PTX3 levels were analysed by enzyme-linked immunosorbent assays in sera from unselected patients with AAV and compared with patients with systemic lupus erythematosus (SLE, n = 130), other connective tissue diseases (CTDs, n = 97) and matched healthy controls (n = 97). Optical density (OD) cut-off for positive anti-PTX3 antibodies was determined by ROC curve analysis and set as 0. Read More

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September 2021

Development and characterization of a unique anti-IgE mouse monoclonal antibody cross-reactive between human and canine IgE.

Immun Inflamm Dis 2021 Sep 17. Epub 2021 Sep 17.

Animal Allergy Clinical Laboratories Inc., Sagamihara, Kanagawa, Japan.

Background: The efficacy assessment of human anti-IgE monoclonal antibodies (mAbs) in animal models before clinical trials is hampered due to the lack of cross-reactivity of anti-IgE mAbs between species.

Objective: We developed CRE-DR (an anti-dog IgE monoclonal antibody), an anti-IgE mouse mAb that recognizes canine and human IgE, and then examined its IgE specificity and cross-reactivity between three animal and human species.

Methods: After mouse immunization with a synthetic peptide derived from canine IgE ( NTNDWIEGETYYC ), we generated a hybridoma producing CRE-DR. Read More

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September 2021

Multi-functional antibody profiling for malaria vaccine development and evaluation.

Expert Rev Vaccines 2021 Sep 17. Epub 2021 Sep 17.

Burnet Institute, Melbourne, Australia.

IntroductionA vaccine would greatly accelerate current global efforts towards malaria elimination. While a partially efficacious vaccine has been achieved for , a major bottleneck in developing highly efficacious vaccines is a lack of reliable correlates of protection, and the limited application of assays that quantify functional immune responses to evaluate and down-select vaccine candidates in pre-clinical studies and clinical trials.Areas coveredIn this review we describe the important role of antibodies in immunity against malaria and detail the nature and functional activities of antibodies against malaria. Read More

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September 2021

Serological Screening of Suspected COVID-19 Patients in Pakistan.

J Coll Physicians Surg Pak 2021 01;31(1):S71-S74

Department of Pathology, Mardan Medical Complex, Mardan, Pakistan.

Objective: To determine the antibody levels (IgM and IgG), using ELISA in suspected patients of COVID-19.

Study Design: Descriptive cross-sectional study. Place & Duration of Study: Real Time PCR Diagnostic and Research Laboratory, Peshawar, Khyber Pakhtunkhwa, Pakistan, from May to July 2020. Read More

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January 2021

Saliva SARS-CoV-2 Antibody Prevalence in Children.

Microbiol Spectr 2021 Sep 15:e0073121. Epub 2021 Sep 15.

Department of Pediatric Infectious Diseases, Rheumatology, & Immunology, Emma Children's Hospital, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.

COVID-19 patients produce circulating and mucosal antibodies. In adults, specific saliva antibodies have been detected. Nonetheless, seroprevalence is routinely investigated, while little attention has been paid to mucosal antibodies. Read More

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September 2021

Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky.

Sci Rep 2021 09 14;11(1):18285. Epub 2021 Sep 14.

James Graham Brown Cancer Center, University of Louisville School of Medicine, University of Louisville, Louisville, KY, USA.

Serological assays intended for diagnosis, sero-epidemiologic assessment, and measurement of protective antibody titers upon infection or vaccination are essential for managing the SARS-CoV-2 pandemic. Serological assays measuring the antibody responses against SARS-CoV-2 antigens are readily available. However, some lack appropriate characteristics to accurately measure SARS-CoV-2 antibodies titers and neutralization. Read More

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September 2021

A Comparison of the Performance of SARS-CoV-2 Antibody Assays in Healthcare Workers with COVID-19.

Ir Med J 2021 08 19;114(7):414. Epub 2021 Aug 19.

Genitourinary Medicine and Infectious Diseases Department (GUIDe), St. James's Hospital, Dublin.

Aims Since its emergence, significant interest surrounds the use of SARS-CoV-2 serological tests as an alternative or as an adjunct to molecular testing. However, given the speed of this pandemic, paralleled with the pressure to develop and provide serological tests in an expediated manner, not every assay has undergone the rigorous evaluation that is usually associated with medical diagnostic assays. We aimed to examine the performance of several commercially available SARS-CoV-2 IgG antibody assays among participants with confirmed COVID-19 disease and negative controls. Read More

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Development of in-house, indirect ELISAs for the detection of SARS-CoV-2 spike protein-associated serology in COVID-19 patients in Panama.

PLoS One 2021 14;16(9):e0257351. Epub 2021 Sep 14.

Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Ciudad de Panama, Panama.

COVID-19 is the name of the acute respiratory disease caused by the new coronavirus SARS-CoV-2, a close relative of those that caused the severe outbreaks of SARS and MERS several years ago. Since first appearance on December of 2019, the COVID-19 pandemic has cause extremely high levels of mortality, morbidity, global economic breakdown, and the consequent human suffering. The main diagnostic test for the confirmation of symptomatic individuals is the detection of viral RNA by reverse transcriptase-quantitative real time PCR (RT-PCR). Read More

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September 2021

Development and validation of an enzyme immunoassay for detection and quantification of SARS-CoV-2 salivary IgA and IgG.

medRxiv 2021 Sep 7. Epub 2021 Sep 7.

Oral fluids offer a non-invasive sampling method for the detection of antibodies. Quantification of IgA and IgG antibodies in saliva allows studies of the mucosal and systemic immune response after natural infection or vaccination. We developed and validated an enzyme immunoassay (EIA) to detect and quantify salivary IgA and IgG antibodies against the prefusion-stabilized form of the SARS-CoV-2 spike protein. Read More

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September 2021

Diagnosing COVID-19 in human sera with detected immunoglobulins IgM and IgG by means of Raman spectroscopy.

J Raman Spectrosc 2021 Aug 19. Epub 2021 Aug 19.

Biomedical Engineering Program Universidade Anhembi Morumbi - UAM São Paulo State of São Paulo Brazil.

The severe COVID-19 pandemic requires the development of novel, rapid, accurate, and label-free techniques that facilitate the detection and discrimination of SARS-CoV-2 infected subjects. Raman spectroscopy has been used to diagnose COVID-19 in serum samples of suspected patients without clinical symptoms of COVID-19 but presented positive immunoglobulins M and G (IgM and IgG) assays versus Control (negative IgM and IgG). A dispersive Raman spectrometer (830 nm, 350 mW) was employed, and triplicate spectra were obtained. Read More

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Characterization of N-Terminal Glutamate Cyclization in Monoclonal Antibody and Bispecific Antibody Using Charge Heterogeneity Assays and Hydrophobic Interaction Chromatography.

J Pharm Sci 2021 Sep 10. Epub 2021 Sep 10.

Analytical Sciences, Biopharmaceutical Development, Biopharmaceuticals R&D, AstraZeneca, One Medimmune Way, Gaithersburg, MD 20878.

N-terminal glutamate (E) cyclization to form pyroglutamate (pE) generates charge heterogeneities for mAbs and proteins. Thus far, pE formation rate in lyophilized formulation as compared to in liquid formulation has not been reported. Impact of pE on antibody biological activity has only been predicted or assessed using stressed samples that may contain other confounding degradations besides pE. Read More

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September 2021

Development and Immunogenicity of a Brazilian Glycoconjugate vaccine against Meningococcal W in a Pilot Scale.

Glycoconj J 2021 Sep 13. Epub 2021 Sep 13.

Laboratório de Tecnologia Bacteriana, Fundação Oswaldo Cruz, Bio-ManguinhosRio de Janeiro, Brazil.

Recent changes in the epidemiology of meningococcal have been reported and meningococcal group W (MenW) has become the third most prevalent group isolated in Brazil in the last 10 years. In this study we have developed a conjugate vaccine for MenW using a modified reductive amination conjugation method through a covalent linkage between periodate-oxidized MenW non-O-acetylated polysaccharide and hydrazide-activated monomeric tetanus toxoid. Process control of bulks was done by physicochemical analysis including polysaccharide and protein quantification, high performance liquid chromatography - size exclusion chromatography, capillary electrophoresis, and hydrogen nuclear magnetic resonance. Read More

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September 2021

Alteration of Allergen Fold of Bos d 5 into a Hypoallergenic Vaccine for Immunotherapy of Cow's Milk Allergy.

Int Arch Allergy Immunol 2021 Aug 12:1-12. Epub 2021 Aug 12.

Department of Clinical Laboratory, School of Medical Laboratory, Tianjin Medical University, Tianjin, China.

Background: Cow's milk allergy (CMA) is the most common IgE-mediated food allergy and Bos d 5 is the major allergen in cow's milk proteins. More than 60% of the patients with CMA are sensitized to this protein.

Methods And Results: A recombinant protein, encoded by a synthetic gene and consisting of reassembled Bos d 5 fragments, was expressed in E. Read More

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Rapid and Laboratory SARS-CoV-2 Antibody Testing in High-Risk Hospital Associated Cohorts of Unknown COVID-19 Exposure, a Validation and Epidemiological Study After the First Wave of the Pandemic.

Front Med (Lausanne) 2021 26;8:642318. Epub 2021 Aug 26.

Infectious Diseases Department, Mater Misericordiae University Hospital, Dublin, Ireland.

We aimed to use SARS-CoV-2 antibody tests to assess the asymptomatic seroprevalence of individuals in high-risk hospital cohorts who's previous COVID-19 exposure is unknown; staff, and patients requiring haemodialysis or chemotherapy after the first wave. In a single Center, study participants had five SARS-CoV-2 antibody tests done simultaneously; one rapid diagnostic test (RDT) (Superbio Colloidal Gold IgM/IgG), and four laboratory tests (Roche Elecsys® Anti-SARS-CoV-2 IgG [RE], Abbott Architect i2000SR IgG [AAr], Abbott Alinity IgG [AAl], and Abbott Architect IgM CMIA). To determine seroprevalence, only positive test results on laboratory assay were considered true positives. Read More

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Follicular Helper T (T) Cell Targeting by TLR8 Signaling For Improving HBsAg-Specific B Cell Response In Chronic Hepatitis B Patients.

Front Immunol 2021 26;12:735913. Epub 2021 Aug 26.

Division of Clinical Care and Research, Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, United States.

Identifying signaling pathways that induce B cell response can aid functional cure strategies for chronic hepatitis B infection (CHB). TLR8 activation with ssRNA was shown to enhance follicular helper T cell (T) function leading to improved B cell responses . We investigated whether this mechanism can rescue an exhausted immune response in CHB infection. Read More

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Classification of measles breakthrough cases in an elimination setting using a comprehensive algorithm of laboratory results: why highly sensitive and specific IgM assays are important.

Int J Infect Dis 2021 Sep 8. Epub 2021 Sep 8.

Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.

Objective: In 2006, a measles outbreak occurred in Catalonia (Spain), six years after endemic measles was declared eliminated. The aim of this study was to classify 19 confirmed measles breakthrough cases (BC) using a high-performance avidity assay developed in 2010.

Methods: Serum specimens were tested by indirect IgG, indirect IgM, capture IgM enzyme immunoassay, an endpoint-titer IgG avidity assay, and a plaque reduction neutralization assay. Read More

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September 2021

Overcoming the challenges in diagnosis of AQP4-IgG positive neuromyelitis optica spectrum disorders in resource poor settings using an indigenized and cost effective cell based assay.

J Neuroimmunol 2021 Sep 2;360:577706. Epub 2021 Sep 2.

Center for Advanced Neurological Research, KS Hegde Medical Academy, Nitte University, Mangalore, India.

Background: Diagnosis of neuromyelitis optica spectrum disorders (NMOSD) in India is hindered by limited access to cost effective and sensitive assays for detection of aquaporin-4 antibody (AQP4-IgG) in India.

Objective: To develop a cost effective, sensitive, cell based assay (CBA) for detection of AQP4-IgG and to evaluate the serological status in patients with NMOSD diagnosed by 2015 diagnostic criteria.

Method: Stably transfected Chinese hamster ovary (CHO) cell line expressing aquaporin M23 isomer was established. Read More

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September 2021

Blocking of Birc3/TLR4/Myd88 signaling protects carbapenem-resistant klebsiella pneumoniae in a mouse model of infection.

Transpl Immunol 2021 Sep 6;69:101464. Epub 2021 Sep 6.

Department of Clinical Laboratory, The Second People's Hospital of Lanzhou City, Lanzhou 730046, China.

Background: Klebsiella pneumonia (KP) and carbapenem-resistant Klebsiella pneumonia (CRKP) lung infections significantly increase the morbidity and mortality of pneumonia. Recent studies have shown that baculoviral IAP repeat-containing 3 (Birc3) plays an important role in the prevention and treatment of pneumonia. However, the role of Birc3 in CRKP-induced pneumonia has not been widely reported. Read More

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September 2021