J Am Chem Soc 2016 06 10;138(22):7143-50. Epub 2016 May 10.
Department of Chemistry & Biochemistry, Swarthmore College , 500 College Avenue, Swarthmore, Pennsylvania 19081, United States.
Fluorogenic dyes such as FlAsH and ReAsH are used widely to localize, monitor, and characterize proteins and their assemblies in live cells. These bis-arsenical dyes can become fluorescent when bound to a protein containing four proximal Cys thiols-a tetracysteine (Cys4) motif. Yet the mechanism by which bis-arsenicals become fluorescent upon binding a Cys4 motif is unknown, and this nescience limits more widespread application of this tool. Read More