117 results match your criteria identifies vimentin

The Fibronectin Expression Determines the Distinct Progressions of Malignant Gliomas via Transforming Growth Factor-Beta Pathway.

Int J Mol Sci 2021 Apr 6;22(7). Epub 2021 Apr 6.

Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.

Due to the increasing incidence of malignant gliomas, particularly glioblastoma multiforme (GBM), a simple and reliable GBM diagnosis is needed to screen early the death-threaten patients. This study aimed to identify a protein that can be used to discriminate GBM from low-grade astrocytoma and elucidate further that it has a functional role during malignant glioma progressions. To identify proteins that display low or no expression in low-grade astrocytoma but elevated levels in GBM, glycoprotein fibronectin (FN) was particularly examined according to the mining of the Human Protein Atlas. Read More

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Histamine augments collagen content via H1 receptor stimulation in cultures of myofibroblasts taken from wound granulation tissue.

Mol Cell Biochem 2021 Feb 23;476(2):1083-1092. Epub 2020 Nov 23.

Laboratory of Connective Tissue Metabolism, Department of Pathophysiology, Chair of General and Experimental Pathology Medical University of Lodz, ul. Żeligowskiego 7/9, Lodz, Poland.

The inflammatory reaction influences the deposition of collagen within wound granulation tissue. The aim of the present study is to determine whether histamine acting directly on myofibroblasts derived from wound granulation tissue may influence collagen deposition. It also identifies the histamine receptor involved in this process. Read More

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February 2021

Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway.

Front Endocrinol (Lausanne) 2020 30;11:568436. Epub 2020 Sep 30.

Department of Otorhinolaryngology, Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan.

Oral squamous cell carcinoma (OSCC) that comprises about 90% of all oral cancer cases is associated with poor prognosis due to its highly metastatic nature. The majority of OSCC treatment options are related detrimental side-effects. The present study aimed at deciphering the effects of a bioactive phytochemical, sodium danshensu, on human oral cancer cell metastasis. Read More

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The HNRNPA2B1-MST1R-Akt axis contributes to epithelial-to-mesenchymal transition in head and neck cancer.

Lab Invest 2020 12 15;100(12):1589-1601. Epub 2020 Jul 15.

Lab No. 213, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, Madhya Pradesh, India.

The deregulation of splicing factors and alternative splicing are increasingly viewed as major contributory factors in tumorigenesis. In this study, we report overexpression of a key splicing factor, heterogeneous nuclear ribonucleoprotein A2B1 (HNRNPA2B1), and thereby misregulation of alternative splicing, which is associated with the poor prognosis of head and neck cancer (HNC). The role of HNRNPA2B1 in HNC tumorigenesis via deregulation of alternative splicing is not well understood. Read More

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December 2020

Cell surface processing of the P1 adhesin of Mycoplasma pneumoniae identifies novel domains that bind host molecules.

Sci Rep 2020 04 14;10(1):6384. Epub 2020 Apr 14.

The ithree institute, University of Technology Sydney, PO Box 123, Broadway, NSW, 2007, Australia.

Mycoplasma pneumoniae is a genome reduced pathogen and causative agent of community acquired pneumonia. The major cellular adhesin, P1, localises to the tip of the attachment organelle forming a complex with P40 and P90, two cleavage fragments derived by processing Mpn142, and other molecules with adhesive and mobility functions. LC-MS/MS analysis of M. Read More

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Microvasculature partial endothelial mesenchymal transition in early posttransplant biopsy with acute tubular necrosis identifies poor recovery renal allografts.

Am J Transplant 2020 09 12;20(9):2400-2412. Epub 2020 Apr 12.

Sorbonne Université, Institut national de la santé et de la recherche médicale (INSERM), Unité mixte de recherche (UMR), Paris, France.

Acute tubular necrosis (ATN), a frequent histopathological feature in the early post-renal transplant biopsy, affects long-term graft function. Appropriate markers to identify patients at risk of no or incomplete recovery after delayed graft function are lacking. In this study, we first included 41 renal transplant patients whose biopsy for cause during the first month after transplantation showed ATN lesions. Read More

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September 2020

Improved filtration method to isolate pure populations of primary bovine endometrial epithelial and stromal cells for immunological studies.

Vet Res Commun 2020 Feb 21;44(1):29-39. Epub 2020 Feb 21.

Comparative Immunology Group, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland.

Objectives: Isolation and culture of distinct primary endometrial cells are key to reliable in-vitro models to investigate the uterine immune response and optimse new disease interventions. Details on the isolation method and purity of distinct cell populations is lacking in currently available protocols leading to inconsistent results across laboratories.

Methods: Bovine endometrial tissue from non-pregnant bovine uteri were collected immediately post-mortem and separated using differential size filtering. Read More

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February 2020

Characterizing hand and wrist ultrasound pattern in primary Sjögren's syndrome: a case-control study.

Clin Rheumatol 2020 Jun 19;39(6):1907-1918. Epub 2020 Feb 19.

Rheumatology Division, Hospital das Clinicas, Faculdade de Medicina da Universidade de Sao Paulo (HCFMUSP), Av. Dr. Arnaldo, 455, 3° andar, sala 3190, Cerqueira Cesar, Sao Paulo, SP, 01246-903, Brazil.

Introduction/objectives: To evaluate the clinical relevance of high-resolution hand and wrist ultrasound (US) findings and their possible associations with anti-citrullinated peptide antibodies in primary Sjögren's syndrome (pSS).

Methods: Ninety-seven consecutive pSS patients (American-European Consensus Group, 2002) without meeting the American College of Rheumatology (ACR) criteria (1987) for rheumatoid arthritis (RA); 20 RA patients (ACR/European League Against Rheumatism (EULAR) criteria, 2010); and 80 healthy individuals with comparable age, gender, and ethnicity were enrolled in a case-control study. Disease activity was assessed by EULAR Sjögren's Syndrome Disease Activity Index (ESSDAI). Read More

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Quantitative SUMO proteomics identifies PIAS1 substrates involved in cell migration and motility.

Nat Commun 2020 02 11;11(1):834. Epub 2020 Feb 11.

Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Québec, Canada.

The protein inhibitor of activated STAT1 (PIAS1) is an E3 SUMO ligase that plays important roles in various cellular pathways. Increasing evidence shows that PIAS1 is overexpressed in various human malignancies, including prostate and lung cancers. Here we used quantitative SUMO proteomics to identify potential substrates of PIAS1 in a system-wide manner. Read More

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February 2020

Integrated analysis identifies DUSP5 as a novel prognostic indicator for thyroid follicular carcinoma.

Thorac Cancer 2020 02 10;11(2):336-345. Epub 2019 Dec 10.

Department of Endocrinology, Qilu Hospital, Shandong University, Jinan, China.

Background: Differentiated thyroid cancer involves thyroid follicular carcinoma (FTC) and papillary thyroid carcinoma (PTC). Patients with FTC have a worse prognosis than those with PTC for early metastasis through blood of FTC. However, the mechanism of poor prognosis of FTC is still unclear. Read More

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February 2020

A Proteomic Study of Atherosclerotic Plaques in Men with Coronary Atherosclerosis.

Diagnostics (Basel) 2019 Nov 7;9(4). Epub 2019 Nov 7.

Research Institute of Internal and Preventive Medicine - Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630089 Novosibirsk, Russia.

Background: To study the changes in protein composition of atherosclerotic plaques at different stages of their development in coronary atherosclerosis using proteomics.

Methods: The object of research consisted of homogenates of atherosclerotic plaques from coronary arteries at different stages of development, obtained from 15 patients. Plaque proteins were separated by two-dimensional electrophoresis. Read More

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November 2019

F-Choline PET/CT Identifies High-Grade Prostate Cancer Lesions Expressing Bone Biomarkers.

J Clin Med 2019 Oct 11;8(10). Epub 2019 Oct 11.

Department of Biomedicine and Prevention, University of Rome "Tor Vergata", 00133 Rome, Italy.

The main aim of this study was to investigate the possible association between F-choline uptake and histopathological features of prostate biopsies such as the Gleason Group and the expression of both epithelial to mesenchymal transition (vimentin) and bone mineralization (bone morphogenetics protein (BMP)-2, runt-related transcription factor 2 (RUNX2), receptor activator of nuclear factor-κB ligand (RANKL), vitamin D receptor (VDR), and pentraxin 3 (PTX3) in situ biomarkers. To this end, we enrolled 79 consecutive prostate cancer patients that underwent both the F-choline PET/CT analysis and the prostate bioptic procedure. The standardized uptake value (SUV) average values were collected from F-choline PET/CT analysis whereas Gleason Group and immunostaining data were collected from paraffin-embedded sections. Read More

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October 2019

Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling.

Mol Biol Cell 2019 06 17;30(12):1437-1450. Epub 2019 Apr 17.

Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India.

Blood vessel formation requires endothelial cell (EC) migration that depends on dynamic remodeling of the cytoskeleton. Rudhira/Breast Carcinoma Amplified Sequence 3 (BCAS3) is a cytoskeletal protein essential for EC migration and sprouting angiogenesis during mouse development and is implicated in metastatic disease. Here, we report that Rudhira mediates cytoskeleton organization and dynamics during EC migration. Read More

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Low tumour PPM1H indicates poor prognosis in colorectal cancer via activation of cancer-associated fibroblasts.

Br J Cancer 2019 05 16;120(10):987-995. Epub 2019 Apr 16.

Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Rd., Shanghai, 200433, China.

Background: Vimentin (VIM) is considered a prognostic marker in colorectal cancer (CRC). Our aim is to identify genes that fulfil a "X-low implies VIM-high" Boolean relationship and to evaluate their prognostic value and potential mechanism.

Methods: Potential biomarkers related to VIM expression were searched using a bioinformatics approach across gene-expression arrays. Read More

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Inhibition of didscoidin domain receptor 1 reduces epithelial-mesenchymal transition and induce cell-cycle arrest and apoptosis in prostate cancer cell lines.

J Cell Physiol 2019 11 8;234(11):19539-19552. Epub 2019 Apr 8.

Department of Clinical Biochemistry, School of Pharmacy & Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Didscoidin domain receptor 1 (DDR1) is involved in the progression of prostate cancer metastasis through stimulation of epithelial-mesenchymal transition (EMT). So DDR1 inhibition can be a helpful target for cancer metastasis prevention. So, we studied the effects of DDR1 inhibition on EMT as well as induction of cell-cycle arrest and apoptosis in prostate cancer cell lines. Read More

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November 2019

The induction of core pluripotency master regulators in cancers defines poor clinical outcomes and treatment resistance.

Oncogene 2019 05 11;38(22):4412-4424. Epub 2019 Feb 11.

Northern Institute for Cancer Research, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK.

Stem cell characteristics have been associated with treatment resistance and poor prognosis across many cancer types. The ability to induce and regulate the pathways that sustain these characteristic hallmarks of lethal cancers in a novel in vitro model would greatly enhance our understanding of cancer progression and treatment resistance. In this work, we present such a model, based simply on applying standard pluripotency/embryonic stem cell media alone. Read More

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DPYSL3 modulates mitosis, migration, and epithelial-to-mesenchymal transition in claudin-low breast cancer.

Proc Natl Acad Sci U S A 2018 12 29;115(51):E11978-E11987. Epub 2018 Nov 29.

Lester and Sue Smith Breast Center, Baylor College of Medicine, Houston, TX 77030;

A Clinical Proteomic Tumor Analysis Consortium (CPTAC) proteogenomic analysis prioritized dihydropyrimidinase-like-3 (DPYSL3) as a multilevel (RNA/protein/phosphoprotein) expression outlier specific to the claudin-low (CLOW) subset of triple-negative breast cancers. A PubMed informatics tool indicated a paucity of data in the context of breast cancer, which further prioritized DPYSL3 for study. DPYSL3 knockdown in DPYSL3-positive ([Formula: see text]) CLOW cell lines demonstrated reduced proliferation, yet enhanced motility and increased expression of epithelial-to-mesenchymal transition (EMT) markers, suggesting that DPYSL3 is a multifunctional signaling modulator. Read More

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December 2018

Glutathione reductase-mediated thiol oxidative stress suppresses metastasis of murine melanoma cells.

Free Radic Biol Med 2018 12 4;129:256-267. Epub 2018 Aug 4.

Zhejiang Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou, Zhejiang Province 310022, China; Zhejiang Key Laboratory of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang Province 310022, China. Electronic address:

Malignant melanoma is a highly metastatic and life-threatening cancer. Reactive oxygen species (ROS) play important roles in cancer initiation and progression including metastasis. It has been reported that the oxidative stress spontaneously generated in circulating melanoma cells was able to suppress distant metastasis in vivo. Read More

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December 2018

USP10 regulates the stability of the EMT-transcription factor Slug/SNAI2.

Biochem Biophys Res Commun 2018 08 1;502(4):429-434. Epub 2018 Jun 1.

Department of Physiology and Pharmacology, Karolinska Institutet, Biomedicum, Tomtebodavägen 16, 171 65, Stockholm, Sweden. Electronic address:

Epithelial-to-mesenchymal transition (EMT) is a fundamental mechanism governing the switch of cells from an epithelial to a motile mesenchymal-like state. This transdifferentiation is regulated by key transcription factors, including Slug. The stability and function of Slug can be regulated by multiple mechanisms, including ubiquitin-mediated post-translational modifications. Read More

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Label-free quantitative proteomic analysis identifies CTNNB1 as a direct target of FOXP3 in gastric cancer cells.

Oncol Lett 2018 May 15;15(5):7655-7660. Epub 2018 Mar 15.

Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.

Forkhead box protein 3 (FOXP3) is expressed in numerous types of tumor cell and is associated with tumor progression and prognosis. A previous study reported that FOXP3 inhibited cellular proliferation and induced apoptosis of gastric cancer (GC) cells by activating the apoptosis signaling pathway. In the present study, label-free quantitative proteomic analysis and chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) was performed to investigate the mechanism by which the anticancer role of FOXP3 was mediated and the proteins that with which it may interact. Read More

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A functional genomic screen in vivo identifies CEACAM5 as a clinically relevant driver of breast cancer metastasis.

NPJ Breast Cancer 2018 30;4. Epub 2018 Apr 30.

1Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030 USA.

Tumor cells disseminate early in tumor development making metastasis-prevention strategies difficult. Identifying proteins that promote the outgrowth of disseminated tumor cells may provide opportunities for novel therapeutic strategies. Despite multiple studies demonstrating that the mesenchymal-to-epithelial transition (MET) is critical for metastatic colonization, key regulators that initiate this transition remain unknown. Read More

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Phosphoproteomics Analysis Identifies Novel Candidate Substrates of the Nonreceptor Tyrosine Kinase, rc-elated Kinase Lacking C-terminal Regulatory Tyrosine and N-terminal yristoylation ites (SRMS).

Mol Cell Proteomics 2018 05 1;17(5):925-947. Epub 2018 Mar 1.

From the ‡Department of Biochemistry, College of Medicine, 107 Wiggins Road, University of Saskatchewan, Saskatoon S7N 5E5, Saskatchewan, Canada;

SRMS (rc-related kinase lacking C-terminal egulatory tyrosine and N-terminal yristoylation ites), also known as PTK 70 (Protein tyrosine kinase 70), is a non-receptor tyrosine kinase that belongs to the BRK family of kinases (BFKs). To date less is known about the cellular role of SRMS primarily because of the unidentified substrates or signaling intermediates regulated by the kinase. In this study, we used phosphotyrosine antibody-based immunoaffinity purification in large-scale label-free quantitative phosphoproteomics to identify novel candidate substrates of SRMS. Read More

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Invasion of the Brain by Is Mediated by InlF and Host Cell Vimentin.

mBio 2018 02 27;9(1). Epub 2018 Feb 27.

Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA

is a facultative intracellular bacterial pathogen that is frequently associated with food-borne infection. Of particular concern is the ability of to breach the blood-brain barrier, leading to life-threatening meningitis and encephalitis. The mechanisms used by bacterial pathogens to infect the brain are not fully understood. Read More

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February 2018

Autophagy modulates transforming growth factor beta 1 induced epithelial to mesenchymal transition in non-small cell lung cancer cells.

Biochim Biophys Acta Mol Cell Res 2018 May 24;1865(5):749-768. Epub 2018 Feb 24.

Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada; Biology of Breathing Theme, Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, Canada; Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

Lung cancer is considered one of the most frequent causes of cancer-related death worldwide and Non-Small Cell Lung Cancer (NSCLC) accounts for 80% of all lung cancer cases. Autophagy is a cellular process responsible for the recycling of damaged organelles and protein aggregates. Transforming growth factor beta-1 (TGFβ) is involved in Epithelial to Mesenchymal Transition (EMT) and autophagy induction in different cancer models and plays an important role in the pathogenesis of NSCLC. Read More

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An image-based small-molecule screen identifies vimentin as a pharmacologically relevant target of simvastatin in cancer cells.

FASEB J 2018 05 18;32(5):2841-2854. Epub 2018 Jan 18.

Department of Cell Biology and Physiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.

Vimentin is a cytoskeletal intermediate filament protein that is expressed in mesenchymal cells and cancer cells during the epithelial-mesenchymal transition. The goal of this study was to identify vimentin-targeting small molecules by using the Tocriscreen library of 1120 biochemically active compounds. We monitored vimentin filament reorganization and bundling in adrenal carcinoma SW13 vimentin-positive (SW13-vim) cells via indirect immunofluorescence. Read More

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A comparative study of the neural stem cell niche in the adult hypothalamus of human, mouse, rat and gray mouse lemur (Microcebus murinus).

J Comp Neurol 2018 06 25;526(9):1419-1443. Epub 2018 Mar 25.

Inserm, Jean-Pierre Aubert Research Center, Development and Plasticity of the Neuroendocrine Brain, Lille Cedex, France.

The adult brain contains niches of neural stem cells that continuously add new neurons to selected circuits throughout life. Two niches have been extensively studied in various mammalian species including humans, the subventricular zone of the lateral ventricles and the subgranular zone of the hippocampal dentate gyrus. Recently, studies conducted mainly in rodents have identified a third neurogenic niche in the adult hypothalamus. Read More

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MiR-181a promotes epithelial to mesenchymal transition of prostate cancer cells by targeting TGIF2.

Eur Rev Med Pharmacol Sci 2017 Nov;21(21):4835-4843

Department of Urology, Huashan Hospital Affiliated to Fudan University, Shanghai, China.

Objective: Prostate cancer is the most commonly diagnosed cancer, and metastatic prostate cancer often leads to poor outcomes for patients. During the metastasis processes, cancer cells acquire a migratory and invasive phenotype. Epithelial to mesenchymal transition (EMT) has been implicated in multiple processes of prostate cancer development including migration, chemoresistance, and carcinogenesis. Read More

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November 2017

Association of Distinct Fine Specificities of Anti-Citrullinated Peptide Antibodies With Elevated Immune Responses to Prevotella intermedia in a Subgroup of Patients With Rheumatoid Arthritis and Periodontitis.

Arthritis Rheumatol 2017 12 30;69(12):2303-2313. Epub 2017 Oct 30.

University of Oxford, Oxford, UK.

Objective: In addition to the long-established link with smoking, periodontitis (PD) is a risk factor for rheumatoid arthritis (RA). This study was undertaken to elucidate the mechanism by which PD could induce antibodies to citrullinated peptides (ACPAs), by examining the antibody response to a novel citrullinated peptide of cytokeratin 13 (CK-13) identified in gingival crevicular fluid (GCF), and comparing the response to 4 other citrullinated peptides in patients with RA who were well-characterized for PD and smoking.

Methods: The citrullinomes of GCF and periodontal tissue from patients with PD were mapped by mass spectrometry. Read More

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December 2017

Comparative Proteomics Analysis Identifies Cdc42-Cdc42BPA Signaling as Prognostic Biomarker and Therapeutic Target for Colon Cancer Invasion.

J Proteome Res 2018 01 7;17(1):265-275. Epub 2017 Nov 7.

Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632, China.

Metastasis is one of the major causes of treatment failure in the patients with colon cancer. The aim of our study is to find key proteins and pathways that drive invasion and metastasis in colon cancer. Eight rounds of selection of cancer cells invading through matrigel-coated chamber were performed to obtain highly invasive colon cancer sublines HCT116-I8 and RKO-I8. Read More

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January 2018

N-cadherin identifies human endometrial epithelial progenitor cells by in vitro stem cell assays.

Hum Reprod 2017 11;32(11):2254-2268

The Ritchie Centre, Hudson Institute of Medical Research, Clayton, Victoria 3168, Australia.

Study Question: Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity using in vitro assays?

Summary Answer: N-cadherin isolates clonogenic, self-renewing human endometrial epithelial progenitor cells with high proliferative potential that differentiate into cytokeratin+ gland-like structures in vitro and identifies their location in some cells of gland profiles predominantly in basalis endometrium adjacent to the myometrium.

What Is Known Already: Human endometrium contains a small population of clonogenic, self-renewing epithelial cells with high proliferative potential that differentiate into large gland-like structures, but their identity and location is unknown. Stage-specific embryonic antigen-1 (SSEA-1) distinguishes the epithelium of basalis from functionalis and is a marker of human post-menopausal (Post-M) endometrial epithelium. Read More

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November 2017