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Knee strength outcomes in adolescents by age and sex during late-stage rehabilitation after anterior cruciate ligament reconstruction.

Phys Ther Sport 2021 Jul 18;51:102-109. Epub 2021 Jul 18.

Connecticut Children's, Sports Physical Therapy, Farmington, CT, United States.

Objective: To determine if knee strength differences exist according to age group and sex among adolescents during late-stage anterior cruciate ligament reconstruction (ACLR) rehabilitation.

Design: Retrospective cohort study.

Methods: Consecutive patients who had undergone ACLR with a hamstring autograft completed isokinetic knee strength assessment. Read More

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Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics.

Cell Rep 2021 06 8;35(13):109305. Epub 2021 Jun 8.

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. Electronic address:

The human leukocyte antigen (HLA)-bound viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve by acquiring mutations, it is essential to identify a range of presented viral antigens. Using HLA peptidomics, we are able to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-derived peptides presented by highly prevalent HLA class I (HLA-I) molecules by using infected cells as well as overexpression of SARS-CoV-2 genes. Read More

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R869C mutation in molecular motor gene is involved in dementia with Lewy bodies.

Alzheimers Dement (Amst) 2021 4;13(1):e12143. Epub 2021 Jun 4.

The Genomic Research Laboratory for Neurodegeneration, Neurological Institute Tel Aviv Sourasky Medical Center Tel Aviv Israel.

Introduction The: -N370S mutation is one of the most frequent risk factors for dementia with Lewy bodies (DLB) and Parkinson's disease (PD). We looked for genetic variations that contribute to the outcome in N370S-carriers, whether PD or DLB.

Methods: Whole-genome sequencing of 95 Ashkenazi-N370S-carriers affected with either DLB (n = 19) or PD (n = 76) was performed, and 564 genes related to dementia and PD analyzed. Read More

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Sequence of the supernumerary B chromosome of maize provides insight into its drive mechanism and evolution.

Proc Natl Acad Sci U S A 2021 Jun;118(23)

Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO 65211.

B chromosomes are enigmatic elements in thousands of plant and animal genomes that persist in populations despite being nonessential. They circumvent the laws of Mendelian inheritance but the molecular mechanisms underlying this behavior remain unknown. Here we present the sequence, annotation, and analysis of the maize B chromosome providing insight into its drive mechanism. Read More

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Treatment of Barth Syndrome by Cardiolipin Manipulation (CARDIOMAN) With Bezafibrate: Protocol for a Randomized Placebo-Controlled Pilot Trial Conducted in the Nationally Commissioned Barth Syndrome Service.

JMIR Res Protoc 2021 May 31;10(5):e22533. Epub 2021 May 31.

Bristol Trials Centre (Clinical Trials and Evaluation Unit), Bristol Medical School, University of Bristol, Bristol, United Kingdom.

Background: Barth syndrome is a rare, life-threatening, X-linked recessive genetic disease that predominantly affects young males and is caused by abnormal mitochondrial lipid metabolism. Currently, there is no definitive treatment for Barth syndrome other than interventions to ameliorate acute symptoms, such as heart failure, cardiac arrhythmias, neutropenia, and severe muscle fatigue. Previous mechanistic studies have identified the lipid-lowering drug bezafibrate as a promising potential treatment; however, to date, no human trials have been performed in this population. Read More

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Selection for CD26 and CD49A Cells From Pluripotent Stem Cells-Derived Islet-Like Clusters Improves Therapeutic Activity in Diabetic Mice.

Front Endocrinol (Lausanne) 2021 5;12:635405. Epub 2021 May 5.

Kadimastem Ltd., Weizmann Science Park, Ness Ziona, Israel.

Background: Cell therapy of diabetes aims at restoring the physiological control of blood glucose by transplantation of functional pancreatic islet cells. A potentially unlimited source of cells for such transplantations would be islet cells derived from an differentiation of human pluripotent stem cells (hESC/hiPSC). The islet-like clusters (ILC) produced by the known differentiation protocols contain various cell populations. Read More

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Trends in, and predictors of, swallowing and social eating outcomes in head and neck cancer survivors: A longitudinal analysis of head and neck 5000.

Oral Oncol 2021 Jul 20;118:105344. Epub 2021 May 20.

Population Health Sciences Institute, Newcastle University Centre for Cancer, Newcastle University, United Kingdom. Electronic address:

Objective: To investigate the recovery trajectory and predictors of outcome for swallowing difficulties following head and neck cancer treatment in a large prospective cohort.

Materials And Methods: Data from 5404 participants of the Head and Neck 5000 study were collected from 2011 to 2014. Patient-reported swallowing was measured using the EORTC HN35, recorded at baseline (pre-treatment) and 4 and 12 months post-baseline. Read More

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MetaGSCA: A tool for meta-analysis of gene set differential coexpression.

PLoS Comput Biol 2021 May 4;17(5):e1008976. Epub 2021 May 4.

Department of Biostatistics, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.

Analyses of gene set differential coexpression may shed light on molecular mechanisms underlying phenotypes and diseases. However, differential coexpression analyses of conceptually similar individual studies are often inconsistent and underpowered to provide definitive results. Researchers can greatly benefit from an open-source application facilitating the aggregation of evidence of differential coexpression across studies and the estimation of more robust common effects. Read More

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Chromatin accessibility and translational landscapes of tea plants under chilling stress.

Hortic Res 2021 May 1;8(1):96. Epub 2021 May 1.

College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, 350002, China.

Plants have evolved regulatory mechanisms at multiple levels to regulate gene expression in order to improve their cold adaptability. However, limited information is available regarding the stress response at the chromatin and translational levels. Here, we characterize the chromatin accessibility, transcriptional, and translational landscapes of tea plants in vivo under chilling stress for the first time. Read More

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A Systematic Review of the Social Network Strategy to Optimize HIV Testing in Key Populations to End the Epidemic in the United States.

AIDS Behav 2021 Apr 19. Epub 2021 Apr 19.

Center for Learning and Innovation, San Francisco Department of Public Health, 25 Van Ness, Suite 500, San Francisco, CA, 94102, USA.

The United States (U.S.) has a plan to end the HIV epidemic by 2030. Read More

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Viral lower respiratory tract infections-strict admission guidelines for young children can safely reduce admissions.

Eur J Pediatr 2021 Aug 8;180(8):2473-2483. Epub 2021 Apr 8.

Department of Pediatric and Adolescent Medicine, Akershus University Hospital, Sykehusveien 25, 1478, Nordbyhagen, Norway.

Viral lower respiratory tract infection (VLRTI) is the most common cause of hospital admission among small children in high-income countries. Guidelines to identify children in need of admission are lacking in the literature. In December 2012, our hospital introduced strict guidelines for admission. Read More

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Self-Assembly of Nanovoids in Si Microcrystals Epitaxially Grown on Deeply Patterned Substrates.

Cryst Growth Des 2020 May 8;20(5):2914-2920. Epub 2020 Apr 8.

L-NESS, Dipartimento di Fisica, Politecnico di Milano, Via Anzani 42, 22100 Como, Italy.

We present an experimental and theoretical analysis of the formation of nanovoids within Si microcrystals epitaxially grown on Si patterned substrates. The growth conditions leading to the nucleation of nanovoids have been highlighted, and the roles played by the deposition rate, substrate temperature, and substrate pattern geometry are identified. By combining various scanning and transmission electron microscopy techniques, it has been possible to link the appearance pits of a few hundred nanometer width at the microcrystal surface with the formation of nanovoids within the crystal volume. Read More

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Persistent variations of blood DNA methylation associated with treatment exposures and risk for cardiometabolic outcomes in long-term survivors of childhood cancer in the St. Jude Lifetime Cohort.

Genome Med 2021 Apr 6;13(1):53. Epub 2021 Apr 6.

Department of Epidemiology and Cancer Control, St. Jude Children's Research Hospital, 262 Danny Thomas Place, MS 735, Memphis, TN, 38105, USA.

Background: It is well-established that cancer treatment substantially increases the risk of long-term adverse health outcomes among childhood cancer survivors. However, there is limited research on the underlying mechanisms. To elucidate the pathophysiology and a possible causal pathway from treatment exposures to cardiometabolic conditions, we conducted epigenome-wide association studies (EWAS) to identify the DNA methylation (DNAm) sites associated with cancer treatment exposures and examined whether treatment-associated DNAm sites mediate associations between specific treatments and cardiometabolic conditions. Read More

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Pediatric -Methyl-d-Aspartate (NMDA) Receptor Encephalitis, With and Without Herpes Encephalitis.

J Child Neurol 2021 Apr 5:8830738211002679. Epub 2021 Apr 5.

Division of Pediatric Neurology, Department of Pediatrics, Children's Hospital of Alabama, 9968University of Alabama at Birmingham, Birmingham, AL, USA.

Objective: To compare clinical, diagnostic, management, and outcome factors in children with anti--methyl-d-aspartate receptor (NMDAR) encephalitis and a history of herpes simplex encephalitis (HSE) to children with NMDAR encephalitis without a history of HSE.

Methods: All patients with anti-NMDAR antibodies in cerebrospinal fluid treated at our institution between 2012 and 2019 were identified and divided into those with a history of HSE (HSE+NMDAR group) and those without a history of HSE (NMDAR-only group). Demographic data, clinical characteristics, immunotherapy, and outcome data were collected on all patients and compared between the 2 groups. Read More

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Self-harm and the COVID-19 pandemic: A study of factors contributing to self-harm during lockdown restrictions.

J Psychiatr Res 2021 05 18;137:437-443. Epub 2021 Mar 18.

Centre for Self-harm and Suicide Prevention Research, Derbyshire Healthcare NHS, Foundation Trust, Derby, UK.

Introduction: The COVID-19 pandemic and resulting public health measures may have major impacts on mental health, including on self-harm. We have investigated what factors related to the pandemic influenced hospital presentations following self-harm during lockdown in England.

Method: Mental health clinicians assessing individuals aged 18 years and over presenting to hospitals in Oxford and Derby following self-harm during the period March 23rd to May 17, 2020 recorded whether the self-harm was related to the impact of COVID-19 and, if so, what specific factors were relevant. Read More

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Characterization of the PRODUCTION of ANTHOCYANIN PIGMENT 1 Arabidopsis dominant mutant using DLEMMA dual isotope labeling approach.

Phytochemistry 2021 Jun 23;186:112740. Epub 2021 Mar 23.

Department of Plant Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel. Electronic address:

Stable isotope labeling has emerged as a valuable tool for metabolite identification and quantification. In this study, we employed DLEMMA, a dual stable isotope labeling approach to identify and track phenylpropanoid pathway in Arabidopsis thaliana. Three forms of phenylalanine (Phe), including unlabeled, PheC and PheCH were used as feeding precursors. Read More

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The role of accommodation environments in student mental health and wellbeing.

BMC Public Health 2021 03 23;21(1):573. Epub 2021 Mar 23.

Department of Primary Care and Mental Health, University of Liverpool, Liverpool, UK.

Background: Due to the increasing concern over student mental health and wellbeing, attention has turned to the matter of creating environments, communities, and institutions which enable students to flourish.

Methods: To explore the role of accommodation environments in first year student mental health and wellbeing, eight focus groups were conducted in two universities in North West England and analysed using thematic analysis.

Results: Three overarching themes were identified: 'The betwixt space'; 'Accommodations as vessels to cultivate friendships and communities'; and 'The importance of accommodation-based pastoral staff'. Read More

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Genome-wide association meta-analysis identifies pleiotropic risk loci for aerodigestive squamous cell cancers.

PLoS Genet 2021 03 5;17(3):e1009254. Epub 2021 Mar 5.

University of Salzburg, Department of Biosciences and Cancer Cluster Salzburg, Salzburg, Austria.

Squamous cell carcinomas (SqCC) of the aerodigestive tract have similar etiological risk factors. Although genetic risk variants for individual cancers have been identified, an agnostic, genome-wide search for shared genetic susceptibility has not been performed. To identify novel and pleotropic SqCC risk variants, we performed a meta-analysis of GWAS data on lung SqCC (LuSqCC), oro/pharyngeal SqCC (OSqCC), laryngeal SqCC (LaSqCC) and esophageal SqCC (ESqCC) cancers, totaling 13,887 cases and 61,961 controls of European ancestry. Read More

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Type 1 Pilus - A Multifunctional Tool for Optimized Host Interaction.

Front Microbiol 2021 9;12:615924. Epub 2021 Feb 9.

FG Protein Biochemistry & Cellular Microbiology, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Munich, Germany.

represents a major Gram-positive human pathogen causing bacterial pneumonia, otitis media, meningitis, and other invasive diseases. Several pneumococcal isolates show increasing resistance rates against antibacterial agents. A variety of virulence factors promote pneumococcal pathogenicity with varying importance in different stages of host infection. Read More

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February 2021

Tissue Treg Secretomes and Transcription Factors Shared With Stem Cells Contribute to a Treg Niche to Maintain Treg-Ness With 80% Innate Immune Pathways, and Functions of Immunosuppression and Tissue Repair.

Front Immunol 2020 5;11:632239. Epub 2021 Feb 5.

Centers for Cardiovascular Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, United States.

We used functional -omics angles and examined transcriptomic heterogeneity in CD4Foxp3 regulatory T cells (Treg) from spleen (s-Treg), lymph nodes (LN-Treg), intestine (int-Treg), and visceral adipose tissue (VAT-Treg), and made significant findings: Five new shared Treg genes including NIBAN, TNFRSF1b, DUSP4,VAV2, and KLRG1, and 68 new signatures are identified. Among 27 signaling pathways shared in four tissue Treg, 22 pathways are innate immune pathways (81.5%); s-Treg, LN-Treg, int-Treg, and VAT-Treg have zero, 49, 45, and 116 upregulated pathways, respectively; 12, 7, and 15 out of 373 CD markers are identified as specific for LN-Treg, int-Treg, and VAT-Treg, respectively, which may initiate innate immune signaling; 7, 49, 44, and 79 increased cytokines out of 1176 cytokines are identified for four Treg, respectively, suggesting that Treg have much more secretory proteins/cytokines than IL-10, TGF-β, and IL-35; LN-Treg, int-Treg, and VAT-Treg have 13 additional secretory functions more than s-Treg, found by analyzing 1,706 secretomic genes; 2, 20, 25, and 43 increased transcription factors (TFs) out of 1,496 TFs are identified four Treg, respectively; LN-Treg and int-Treg have increased pyroptosis regulators but VAT-Treg have increased apoptosis regulators; 1, 15, 19, and 31 increased kinases out of 661 kinome are identified for s-Treg, LN-Treg, int-Treg, and VAT-Treg, respectively; comparing with that of s-Treg, LN-Treg, int-Treg, and VAT-Treg increase activated cluster (clusters 1-3) markers; and decrease resting cluster (clusters 4-6) markers; and Treg promote tissue repair by sharing secretomes and TFs AHR, ETV5, EGR1, and KLF4 with stem cells, which partially promote upregulation of all the groups of Treg genes. Read More

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Expert consensus on Didactic Clinical Skills Development for orthodontic curricula.

J Dent Educ 2021 Jun 17;85(6):747-755. Epub 2021 Feb 17.

School of Dentistry, University of Missouri-Kansas City, Kansas City, Missouri, USA.

Purpose/objective: Competence is expected of each beginning dentist and orthodontist. However, the broad definition of competence presents a challenge to academic programs in identifying the level of cognition for students to achieve competence. This study aimed to determine the Didactic Clinical Skills Development curriculum content and competency in predoctoral and advanced education orthodontic programs. Read More

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Increased incidence of chalazion associated with face mask wear during the COVID-19 pandemic.

Am J Ophthalmol Case Rep 2021 Jun 9;22:101032. Epub 2021 Feb 9.

Division of Oculoplastics, Stein Eye Institute, University of California, Los Angeles School of Medicine, 100 Stein Plaza Driveway, Los Angeles, CA, 90095, USA.

Purpose: To determine whether the incidence of chalazion increased significantly in the San Francisco Bay Area and Los Angeles County following the widespread adoption of face mask wear in response to the COVID-19 pandemic.

Methods: This is a retrospective multicenter study of two ophthalmology institutions: a private Oculoplastics practice in San Francisco and the Oculoplastics division of the Stein Eye Institute at the University of California, Los Angeles. All patients seen during the studied time periods with a diagnosis of chalazion or hordeolum were identified through review of electronic medical records and included in the study. Read More

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Development of salutogenic coping skills: experiences with daily challenges among young adults suffering from serious mental illness.

Int J Qual Stud Health Well-being 2021 Dec;16(1):1879369

Department of Health and Caring Science, Faculty of Health and Social Sciences, Western Norway University of Applied Sciences , Bergen, Norway.

Purpose: The purpose of this study is to explore experiences with daily challenges and the development of salutogenic copings skills among young adults with serious mental illness.

Methods: Nine young adults with serious mental illness were interviewed. The interviews were transcribed and subjected to reflexive thematic analysis. Read More

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December 2021

Rapid, Sensitive and Reliable Ricin Identification in Serum Samples Using LC-MS/MS.

Toxins (Basel) 2021 01 22;13(2). Epub 2021 Jan 22.

Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona 74100, Israel.

Ricin, a protein derived from the seeds of the castor bean plant (), is a highly lethal toxin that inhibits protein synthesis, resulting in cell death. The widespread availability of ricin, its ease of extraction and its extreme toxicity make it an ideal agent for bioterrorism and self-poisoning. Thus, a rapid, sensitive and reliable method for ricin identification in clinical samples is required for applying appropriate and timely medical intervention. Read More

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January 2021

Kynurenine-Induced Aryl Hydrocarbon Receptor Signaling in Mice Causes Body Mass Gain, Liver Steatosis, and Hyperglycemia.

Obesity (Silver Spring) 2021 02;29(2):337-349

Norris Cotton Cancer Center, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire, USA.

Objective: The aryl hydrocarbon receptor (AHR) plays a key role in obesity. In vitro studies revealed that the tryptophan metabolite kynurenine (Kyn) activates AHR signaling in cultured hepatocytes. The objective of this study was to determine whether Kyn activated the AHR in mice to induce obesity. Read More

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February 2021

Design of SARS-CoV-2 hFc-Conjugated Receptor-Binding Domain mRNA Vaccine Delivered Lipid Nanoparticles.

ACS Nano 2021 06 22;15(6):9627-9637. Epub 2021 Jan 22.

Laboratory of Precision NanoMedicine, Shmunis School for Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causal agent of COVID-19 and stands at the center of the current global human pandemic, with death toll exceeding one million. The urgent need for a vaccine has led to the development of various immunization approaches. mRNA vaccines represent a cell-free, simple, and rapid platform for immunization, and therefore have been employed in recent studies toward the development of a SARS-CoV-2 vaccine. Read More

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Bacterial contamination of blood products in Africa.

Transfusion 2021 03 19;61(3):767-780. Epub 2021 Jan 19.

Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Background: Bacterial contamination of blood components (notably platelets) remains a leading infectious risk to the blood supply. There has been extensive research in high-income countries to characterize the risk of bacterial contamination along with adoption of strategies to mitigate that risk. By contrast, related data in Africa are lacking. Read More

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Integrated differential DNA methylation and gene expression of formalin-fixed paraffin-embedded uveal melanoma specimens identifies genes associated with early metastasis and poor prognosis.

Exp Eye Res 2021 02 30;203:108426. Epub 2020 Dec 30.

Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, Oslo, Norway. Electronic address:

Purpose: Uveal melanoma (UM) is an aggressive malignancy, in which nearly 50% of the patients die from metastatic disease. Aberrant DNA methylation is recognized as an important epigenomic event in carcinogenesis. Formalin-fixed paraffin-embedded (FFPE) samples represent a valuable source of tumor tissue, and recent technology has enabled the use of these samples in genome-wide DNA methylation analyses. Read More

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February 2021

The genotype distribution, infection stage and drug resistance mutation profile of human immunodeficiency virus-1 among the infected blood donors from five Chinese blood centers, 2014-2017.

PLoS One 2020 21;15(12):e0243650. Epub 2020 Dec 21.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.

Human immunodeficiency virus-1 (HIV-1) exhibits high diversity and complexity in China, challenging the disease surveillance and antiretroviral therapy. Between July 1, 2014 and January 30, 2017, we investigated the profiles of HIV-1 infection stages, genotype distribution and drug resistance mutations (DRMs) using plasma samples from HIV Western blot (WB) confirmed blood donors from five Chinese blood centers (Chongqing, Guangxi, Luoyang, Mianyang, and Urumqi). HIV pol regions consisted of whole protease and partial reverse transcriptase were genotyped and analyzed for DRMs. Read More

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February 2021

DUAL-TASK ASSESSMENT IMPLICATIONS FOR ANTERIOR CRUCIATE LIGAMENT INJURY: A SYSTEMATIC REVIEW.

Int J Sports Phys Ther 2020 Dec;15(6):840-855

Doctor of Physical Therapy Program, Tufts University School of Medicine, Boston, MA, United States.

Background: Several systematic reviews have evaluated the role of dual-task assessment in individuals with concussion. However, no systematic reviews to date have investigated dual-task protocols with implications for individuals with anterior cruciate ligament (ACL) injury or ACL reconstruction (ACLR).

Purpose: To systematically review the evidence on dual-task assessment practices applicable to those with ACL deficiency/ACLR, specifically with the aim to identify motor-cognitive performance costs. Read More

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December 2020