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HIV and Surgery for Degenerative Spine Disease: A Systematic Review.

J Neurol Surg A Cent Eur Neurosurg 2021 Apr 12. Epub 2021 Apr 12.

Health Sciences Postgraduate Program, University of Caxias do Sul, Caxias do Sul, RS, Brazil.

Background And Study Aims:  The objective of this review is to evaluate the incidence of operative treatment, outcomes, and complications of surgery for degenerative spine disease (DSD) on human immunodeficiency virus (HIV) positive patients. Combined antiretroviral treatment led HIV patients to live long enough to develop many chronic conditions common in the uninfected population. Surgery for DSD is one of the most commonly performed neurosurgical procedures. Read More

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Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV.

Nature 2021 Apr 12. Epub 2021 Apr 12.

Technical University of Munich, School of Medicine, Institute of Virology, 81675, Munich, Germany.

The global emergence of SARS-CoV-2 urgently requires an in-depth understanding of molecular functions of viral proteins and their interactions with the host proteome. Several individual omics studies have extended our knowledge of COVID-19 pathophysiology. Integration of such datasets to obtain a holistic view of virus-host interactions and to define the pathogenic properties of SARS-CoV-2 is limited by the heterogeneity of the experimental systems. Read More

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Untargeted GC/MS-based approach for identification of anti-inflammatory alkaloids from Hippeastrum elegans (Amaryllidaceae) using a human neutrophil model.

J Pharm Biomed Anal 2021 Apr 3;199:114061. Epub 2021 Apr 3.

Centro de Estudos Farmacêuticos e Cosméticos (CEFAC), Faculdade de Farmácia, Odontologia e Enfermagem, Departamento de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brazil. Electronic address:

Hippeastrum elegans is an Amaryllidaceae species producing alkaloids with pharmaceutical potential including lycorine and galanthamine. Herein, we developed a non-targeted metabolomic study associated to chemometrics and biological evaluations to identify the H. elegans constituents that were able to reduce the human neutrophils proinflammatory mechanisms. Read More

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Increasing the human chorionic gonadotrophin cut-off to ≤1000 IU/l for starting actinomycin D in post-molar gestational trophoblastic neoplasia developing resistance to methotrexate spares more women multi-agent chemotherapy.

ESMO Open 2021 Apr 9;6(3):100110. Epub 2021 Apr 9.

Department of Medical Oncology, Gestational Trophoblastic Disease Centre, Imperial College Healthcare NHS Trust, Charing Cross Hospital, London, UK. Electronic address:

Background: A human chorionic gonadotropin (hCG) cut-off of ≤300 IU/l for starting actinomycin D (ActD) in post-molar gestational trophoblastic neoplasia (GTN) patients developing methotrexate resistance (MTX-R) reduced the number of women needing toxic multi-agent chemotherapy (etoposide, MTX and ActD alternating weekly with cyclophosphamide and vincristine; EMA/CO) without affecting survival. Here we assess whether an increased hCG cut-off of ≤1000 IU/l spares more women EMA/CO.

Patients And Methods: All post-molar GTN patients treated with first-line methotrexate and folinic acid (MTX/FA) were identified in a national cohort between 2009 and 2016. Read More

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Transcriptional signatures underlying dynamic phenotypic switching and novel disease biomarkers in a linear cellular model of melanoma progression.

Neoplasia 2021 Apr 9;23(4):439-455. Epub 2021 Apr 9.

Pharmacology Department, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address:

Despite advances in therapeutics, the progression of melanoma to metastasis still confers a poor outcome to patients. Nevertheless, there is a scarcity of biological models to understand cellular and molecular changes taking place along disease progression. Here, we characterized the transcriptome profiles of a multi-stage murine model of melanoma progression comprising a nontumorigenic melanocyte lineage (melan-a), premalignant melanocytes (4C), nonmetastatic (4C11-) and metastasis-prone (4C11+) melanoma cells. Read More

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Just a bad flu? Tackling the "infodemic" in Ireland through a comparative analysis of hospitalised cases of COVID-19 and influenza.

Public Health 2021 Mar 6;194:19-24. Epub 2021 Mar 6.

Health Intelligence, Strategic Planning and Transformation, 4th Floor, Jervis House, Jervis Street, Dublin 1, D01 W596, Ireland; Royal College of Surgeons in Ireland, Department of Epidemiology & Public Health Medicine, Beaux Lane House, Mercer Street Lower, Dublin 2, Ireland. Electronic address:

Objectives: COVID-19 infection has been compared to seasonal influenza as an argument against non-pharmacological population-based infection control measures known as "lockdowns". Our study sought to compare disease severity measures for patients in Ireland hospitalised with COVID-19 against those hospitalised with seasonal influenza.

Study Design: This is a retrospective population-based cohort study. Read More

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Single-cell proteomics: A treasure trove in neurobiology.

Biochim Biophys Acta Proteins Proteom 2021 Apr 9:140658. Epub 2021 Apr 9.

Proteomics Unit, Department of Biochemistry, Chemistry Institute, Federal University of Rio de janeiro, 21941-909 Rio de Janeiro, Brazil. Electronic address:

Single-cell analysis came to change the way we look at cell populations. RNA sequencing of single cells allowed us to appreciate the diversity of cell types in the human brain in an unprecedented manner and its power to reveal cell-type specific changes in cell populations has just begun to be explored. In this context, looking at the proteome of single cells promises to bring functional information and contribute to completing the picture. Read More

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Mutual functional dependence of cyclase-associated protein 1 (CAP1) and cofilin1 in neuronal actin dynamics and growth cone function.

Prog Neurobiol 2021 Apr 10:102050. Epub 2021 Apr 10.

Molecular Neurobiology Group, Institute of Physiological Chemistry, University of Marburg, 35032, Marburg, Germany; Center for Mind, Brain and Behavior (CMBB), University of Marburg and Justus-Liebig-University Giessen, 35032, Marburg, Germany; DFG Research Training Group, Membrane Plasticity in Tissue Development and Remodeling, GRK 2213, Philipps-University of Marburg, 35032, Marburg, Germany. Electronic address:

Neuron connectivity depends on growth cones that navigate axons through the developing brain. Growth cones protrude and retract actin-rich structures to sense guidance cues. These cues control local actin dynamics and steer growth cones towards attractants and away from repellents, thereby directing axon outgrowth. Read More

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Cross-reactivity of two human IL-6 family cytokines OSM and LIF explored by protein-protein docking and molecular dynamics simulation.

Biochim Biophys Acta Gen Subj 2021 Apr 9:129907. Epub 2021 Apr 9.

School of Pharmaceutical Sciences, Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Chongqing University, Chongqing 401331, China. Electronic address:

Background: Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are two important pro-inflammatory cytokines of the interleukin-6 (IL-6) family. The two cytokines mediated signaling was recently found to be closely associated with cancer and chronic inflammation, which represent promising therapeutic targets for the treatment of many solid tumors and inflammatory disease. As the most closely related members, cross-reactivity of them may result in undesired activation of off-target cells, leading to toxicity or lack of efficacy of the therapeutic effects. Read More

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Novel compound heterozygous LRBA deletionsin a 6-month old with neonatal diabetes.

Diabetes Res Clin Pract 2021 Apr 9:108798. Epub 2021 Apr 9.

Department of Human Genetics, The University of Chicago, 5841 S. Maryland Ave., G701, Chicago, IL 60637. Electronic address:

We report a 6-month-old boy with antibody-positive insulin-dependent diabetes mellitus. Sequencing identified compound heterozygous deletions of exon 5 and exons 36-37 in LRBA. At three years, he has yet to exhibit any other immune symptoms. Read More

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MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF THE PLAGUE VECTOR XENOPSYLLA BRASILIENSIS.

J Parasitol 2021 Mar;107(2):289-294

Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, Johannesburg, South Africa.

Members of the flea family Pulicidae have been the focus of many studies due to their significance as diseases vectors of medical and veterinary importance and their cosmopolitan distribution. They often exhibit variation in morphological features that can make correct species identification and management challenging. This may also apply to Xenopsylla brasiliensis (Baker, 1904), an important plague vector. Read More

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Trp-Trp acts as a multi-functional blocker for human bitter taste receptors, hTAS2R14, hTAS2R16, hTAS2R43, and hTAS2R46.

Biosci Biotechnol Biochem 2021 Apr 12. Epub 2021 Apr 12.

Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.

Many functional food ingredients activate human bitter taste receptors (hTAS2Rs). In this study, A novel inhibitor, Trp-Trp, for hTAS2R14 was identified by searching for the agonist peptide's analogs. Trp-Trp also inhibited hTAS2R16, hTAS2R43, and hTAS2R46, which share the same agonists with hTAS2R14. Read More

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Candidatus Dirofilaria Hongkongensis Infections in Humans During 2005 to 2020, in Kerala, India.

Am J Trop Med Hyg 2021 Apr 12. Epub 2021 Apr 12.

3Center for Nanoscience and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita University, Cochin, Kerala 682041, India.

We tried to determine the epidemiology and species of human dirofilariasis observed at two tertiary care hospitals in Kerala. We searched the hospital database to identify cases of dirofilariosis from January 2005 to March 2020. Along with human isolates, one dog Dirofilaria isolate was also subjected to PCR and sequencing of pan filarial primers cytochrome oxidase subunits 1 and 12S rDNA. Read More

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Profiling the Tox21 Chemical Collection for Acetylcholinesterase Inhibition.

Environ Health Perspect 2021 Apr 12;129(4):47008. Epub 2021 Apr 12.

Division for Pre-Clinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, USA.

Background: Inhibition of acetylcholinesterase (AChE), a biomarker of organophosphorous and carbamate exposure in environmental and occupational human health, has been commonly used to identify potential safety liabilities. So far, many environmental chemicals, including drug candidates, food additives, and industrial chemicals, have not been thoroughly evaluated for their inhibitory effects on AChE activity. AChE inhibitors can have therapeutic applications (e. Read More

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PP7080 expedites the proliferation and migration of lung adenocarcinoma cells via sponging miR-670-3p and regulating UHRF1BP1.

J Gene Med 2021 Apr 12:e3341. Epub 2021 Apr 12.

Department of oncology, the People's Hospital of Tongcheng, Xianning, Hubei, China.

Background: An increasing body of evidence has revealed that lncRNAs play a significant part in a variety of human cancers; lung adenocarcinoma (LUAD) is included.

Methods: The expression of PP7080, miR-670-3p and UHRF1BP1 in LUAD cells and tissues was detected by using RT-qPCR. The role of PP7080 in LUAD cells was validated by CCK-8, flow cytometry, colony formation, transwell and wound healing assays. Read More

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The anatomical basis behind the neuromodulation effects associated with pulmonary vein isolation.

J Cardiovasc Electrophysiol 2021 Apr 12. Epub 2021 Apr 12.

Liverpool Centre for Cardiovascular Science, University of Liverpool and Liverpool Heart and Chest Hospital, Liverpool, UK.

The anatomical basis underlying the neuromodulation effects seen with pulmonary vein (PV) isolation (PVI) is not fully understood. Left atrial (LA) electro-anatomical maps of 38 patients who underwent catheter cardioneuroablation for vagally mediated bradycarrhythmias (VMB) were studied. During the procedure, LA ganglionic plexi (GPs) were systematically identified and ablated. Read More

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"Hurt people hurt people": Relations between adverse experiences and patterns of cyber and in-person aggression and victimization among urban adolescents.

Aggress Behav 2021 Apr 12. Epub 2021 Apr 12.

Division of Violence Prevention, National Center for Injury Prevention and Control, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

This study identified subgroups of adolescents with distinct patterns of involvement with overt and relational in-person and cyber aggression and victimization. We also assessed subgroup similarities and differences in exposure to adverse childhood experiences (ACEs), exposure to community violence, and trauma symptoms. Using latent class analysis, we identified three subgroups among 265 adolescents (M  = 14. Read More

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Human CD8 T stem cell memory subsets phenotypic and functional characterisation are defined by expression of CD122 or CXCR3.

Eur J Immunol 2021 Apr 12. Epub 2021 Apr 12.

Viral Immunology Systems Program (VISP), The Kirby Institute, University of New South Wales, Sydney, 2052, Australia.

Long-lived T memory stem cells (T ) are key to both naturally-occurring, and vaccine-conferred protection against infection. These cells are characterised by the CD45RA CCR7 CD95 phenotype. Significant heterogeneity within the T population is recognised, but distinguishing surface markers and functional characterisation of potential subsets are lacking. Read More

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Omecamtiv mecarbil evokes diastolic dysfunction and leads to periodic electromechanical alternans.

Basic Res Cardiol 2021 Apr 12;116(1):24. Epub 2021 Apr 12.

Division of Clinical Physiology, Department of Cardiology, Faculty of Medicine, University of Debrecen, 22 Móricz Zsigmond Street, 4032, Debrecen, Hungary.

Omecamtiv mecarbil (OM) is a promising novel drug for improving cardiac contractility. We tested the therapeutic range of OM and identified previously unrecognized side effects. The Ca sensitivity of isometric force production (pCa) and force at low Ca levels increased with OM concentration in human permeabilized cardiomyocytes. Read More

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The landscape of copy number variations in classical Hodgkin lymphoma: a joint KU Leuven and LYSA study on cell-free DNA.

Blood Adv 2021 Apr;5(7):1991-2002

Department of Human Genetics and.

The low abundance of Hodgkin/Reed-Sternberg (HRS) cells in lymph node biopsies in classical Hodgkin lymphoma (cHL) complicates the analysis of somatic genetic alterations in HRS cells. As circulating cell-free DNA (cfDNA) contains circulating tumor DNA (ctDNA) from HRS cells, we prospectively collected cfDNA from 177 patients with newly diagnosed, mostly early-stage cHL in a monocentric study at Leuven, Belgium (n = 59) and the multicentric BREACH study by Lymphoma Study Association (n = 118). To catalog the patterns and frequencies of genomic copy number aberrations (CNAs), cfDNA was sequenced at low coverage (0. Read More

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Distinctive clinical presentation and pathogenic specificities of anti-AK5 encephalitis.

Brain 2021 Apr 12. Epub 2021 Apr 12.

French Reference Center on Paraneoplastic Neurological Syndromes and Autoimmune Encephalitis, Hospices Civils de Lyon, Hôpital Neurologique, Bron, France.

Limbic encephalitis (LE) with antibodies against adenylate kinase 5 (AK5) has been difficult to characterize because of its rarity. In this study, we identified 10 new cases and reviewed 16 previously reported patients, investigating clinical features, IgG subclasses, human leukocyte antigen (HLA), and CSF proteomic profiles. Patients with anti-AK5 LE were mostly men (20/26, 76. Read More

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Plasmodium falciparum Atg18 localizes to the food vacuole via interaction with the multi-drug resistance protein 1 and phosphatidylinositol 3-phosphate.

Biochem J 2021 Apr 12. Epub 2021 Apr 12.

Centre for Cellular and Molecular Biology, Hyderabad, India.

Autophagy, a lysosome-dependent degradative process, does not appear to be a major degradative process in malaria parasites and has a limited repertoire of genes. To better understand the autophagy process, we investigated Plasmodiumfalciparum Atg18 (PfAtg18), a PROPPIN family protein, whose members like S. cerevisiae Atg18 (ScAtg18) and human WIPI2 bind PI3P and play an essential role in autophagosome formation. Read More

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Comparison of The Carrier Frequency of Pathogenic Variants of DMD Gene in an Indian Cohort.

J Neuromuscul Dis 2021 Apr 2. Epub 2021 Apr 2.

Department of Neurology, National Institute of Mental Health and Neurosciences, Bengaluru, India.

Background: Duchenne muscular dystrophy (DMD) is an X-linked disorder caused due to large deletions, duplications,and small pathogenic variants. This article compares the carrier frequency of different pathogenic variants in the DMD gene for the first time in an Indian cohort.

Methods: Ninety-one mothers of genetically confirmed DMD probands are included in this study. Read More

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ARTIFICIAL TERMINATION OF PREGNANCY IN UKRAINE: BIOETHICAL, PHILOSOPHICAL AND RELIGIOUS ASPECTS.

Wiad Lek 2021 ;74(3 cz 2):731-735

BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE.

Objective: The aim: Is to study the levels of influence of the Christian spiritual tradition on the attitude of medical students to abortion, which necessitated a comparative analysis of Christian ethics and the main approaches of secular bioethics regarding artificial termination of pregnancy; analysis of the results of sociological research taking into account the peculiarities of religious self-identification of medical students.

Patients And Methods: Materials and methods: The complex nature of the studied issue necessitated the application of interdisciplinary approaches, philosophical, general scientific and special sociological methods of collecting, processing and analyzing information. Certain differences between Christian moral guidelines and attitudes towards artificial termination of pregnancy were found in a questionnaire study conducted by a sociological group of the Bogomolets National Medical University (hereinafter - NMU) in 2020. Read More

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January 2021

A Smartphone-Based Self-management Intervention for Bipolar Disorder (LiveWell): User-Centered Development Approach.

JMIR Ment Health 2021 Apr 12;8(4):e20424. Epub 2021 Apr 12.

Department of Psychiatry and Behavioral Sciences, Feinberg School of Medicine, Northwestern University, Chicago, IL, United States.

Background: Bipolar disorder is a serious mental illness that results in significant morbidity and mortality. Pharmacotherapy is the primary treatment for bipolar disorder; however, adjunctive psychotherapy can help individuals use self-management strategies to improve outcomes. Yet access to this therapy is limited. Read More

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A role for GLUT3 in glioblastoma cell invasion that is not recapitulated by GLUT1.

Cell Adh Migr 2021 Dec;15(1):101-115

Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.

The multifaceted roles of metabolism in invasion have been investigated across many cancers. The brain tumor glioblastoma (GBM) is a highly invasive and metabolically plastic tumor with an inevitable recurrence. The neuronal glucose transporter 3 (GLUT3) was previously reported to correlate with poor glioma patient survival and be upregulated in GBM cells to promote therapeutic resistance and survival under restricted glucose conditions. Read More

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December 2021

Potential aggregation hot spots in recombinant human keratinocyte growth factor: a computational study.

J Biomol Struct Dyn 2021 Apr 10:1-16. Epub 2021 Apr 10.

Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.

The recombinant human keratinocyte growth factor (rhKGF) is a highly aggregation-prone therapeutic protein. The high aggregation liability of rhKGF is manifested by loss of the monomeric state, and accumulation of the aggregated species even at moderate temperatures. Here, we analyzed the rhKGF for its vulnerability toward aggregation by detection of aggregation-prone regions (APRs) using several sequence-based computational tools including TANGO, ZipperDB, AGGRESCAN, Zyggregator, Camsol, PASTA, SALSA, WALTZ, SODA, Amylpred, AMYPDB, and structure-based tools including SolubiS, CamSol structurally corrected, Aggrescan3D and spatial aggregation propensity (SAP) algorithm. Read More

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in cattle and their human contacts in a gaushala (cattle shelter) from India and its partial 1 gene sequence-based phylogenetic analysis.

Anim Biotechnol 2021 Apr 12:1-10. Epub 2021 Apr 12.

Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India.

Q fever caused by is an important zoonosis and has great public health significance. A total of 905 clinical samples from 387 cattle [serum ( = 387); vaginal swabs ( = 387); milk ( = 131)] and 59 serum samples from humans were collected from gaushala (cattle shelter) and screened for anti- IgG antibodies in the sera using an indirect-ELISA kit. Further, the samples were tested for DNA employing TaqMan real-time and conventional PCR assays targeting the 1 gene. Read More

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Salprzesides A and B: two novel icetexane diterpenes with antiangiogenic activity from Maxim.

Nat Prod Res 2021 Apr 11:1-7. Epub 2021 Apr 11.

Department of Orthopaedics, The Second People's Hospital of Yibin, Sichuan, P.R. China.

Two novel icetexane diterpenes were isolated from Maxim., namely Salprzesides A () and B (), together with two known abietane-type diterpenes respectively identified as sahandinone () and miltirone (). The structures of isolated compounds were determined by UV, IR, HR-ESI-MS, 1D and 2D NMR analysis. Read More

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Rational, unbiased selection of reference genes for pluripotent stem cell-derived cardiomyocytes.

Tissue Eng Part C Methods 2021 Apr 11. Epub 2021 Apr 11.

University of Wisconsin-Madison, Chemical and Biological Engineering, 1415 Engineering Drive, Madison, Wisconsin, United States, 53593;

RT-qPCR is a powerful technique to quantify gene expression via transcript abundance. Expression of target genes is normalized to expression of stable reference genes to account for sample preparation variability. Thus, the identification and validation of stably-expressed reference genes is crucial to making accurate, quantitative, statistical conclusions in gene expression studies. Read More

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