63,362 results match your criteria identified conserved

Analysis of Populus glycosyl hydrolase family I members and their potential role in the ABA treatment and drought stress response.

Plant Physiol Biochem 2021 Apr 5;163:178-188. Epub 2021 Apr 5.

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong, 510520, China. Electronic address:

Glycoside hydrolase family 1 (GH1) β-glucosidases (BGLUs) are encoded by a large number of genes and are involved in many developmental processes and stress responses in plants. Due to their importance in plant growth and development, genome-wide analyses have been conducted in the model plant species Arabidopsis thaliana, rice and maize but not in woody plant species, which have important economic and ecological value. In this study, we systematically analyzed Populus BGLUs (PtBGLUs) and demonstrated the involvement of several genes under stress conditions. Read More

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Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis.

Microb Genom 2021 Apr;7(4)

Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Fiocruz-PR, Algacyr Munhoz Mader street, 3775, Curitiba, Paraná, Brazil.

Nonulosonic acids (NulOs) are a group of nine-carbon monosaccharides with different functions in nature. -acetylneuraminic acid (Neu5Ac) is the most common NulO. It covers the membrane surface of all human cells and is a central molecule in the process of self-recognition via SIGLECS receptors. Read More

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Single-Stranded DNA-Binding Proteins in the Archaea.

Methods Mol Biol 2021 ;2281:23-47

Biomedical Sciences Research Complex, School of Biology, University of St Andrews, St Andrews, UK.

Single-stranded (ss) DNA-binding proteins are found in all three domains of life where they play vital roles in nearly all aspects of DNA metabolism by binding to and stabilizing exposed ssDNA and acting as platforms onto which DNA-processing activities can assemble. The ssDNA-binding factors SSB and RPA are extremely well conserved across bacteria and eukaryotes, respectively, and comprise one or more OB-fold ssDNA-binding domains. In the third domain of life, the archaea, multiple types of ssDNA-binding protein are found with a variety of domain architectures and subunit compositions, with OB-fold ssDNA-binding domains being a characteristic of most, but not all. Read More

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January 2021

A novel gene variant (c.857A>G; p.Tyr286Cys) in an extended family with non‑syndromic deafness 2A.

Mol Med Rep 2021 Jun 13;23(6). Epub 2021 Apr 13.

Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, P.R. China.

Deafness is one of the most common sensory disorders found in humans; notably, >60% of all cases of deafness have been attributed to genetic factors. Variants in potassium voltage‑gated channel subfamily Q member 4 () are etiologically linked to a type of progressive hearing loss, deafness non‑syndromic autosomal dominant 2A (DFNA2A). In the present study, whole‑exome sequencing (WES) was performed on three members of a five‑generation Chinese family with 46 members with hearing loss. Read More

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Whole exome sequencing analysis identifies novel Stargardt disease-related gene mutations in Chinese Stargardt disease and retinitis pigmentosa patients.

Eye (Lond) 2021 Apr 12. Epub 2021 Apr 12.

Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China.

Objectives: To delineate the disease-causing mutations of the Stargardt disease-related genes in Chinese patients diagnosed with Stargardt disease or retinitis pigmentosa (RP) by whole exome sequencing analysis.

Methods: A total of 123 sporadic RP or Stargardt disease patients and 2 Stargardt disease families were recruited. All sporadic patients and the probands of the families were subjected to whole exome sequencing analysis. Read More

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Population genomics and antimicrobial resistance dynamics of Escherichia coli in wastewater and river environments.

Commun Biol 2021 Apr 12;4(1):457. Epub 2021 Apr 12.

Antimicrobial Resistance Unit (ARU), Animal Health Department, Faculty of Veterinary Medicine and VISAVET Health Surveillance Centre, Complutense University of Madrid, Madrid, Spain.

Aquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Read More

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Coevolution underlies GPCR-G protein selectivity and functionality.

Sci Rep 2021 Apr 12;11(1):7858. Epub 2021 Apr 12.

Department of Brain and Cognitive Sciences, DGIST, 333 Techno jungang-daero, Daegu, 42988, Republic of Korea.

G protein-coupled receptors (GPCRs) regulate diverse physiological events, which makes them as the major targets for many approved drugs. G proteins are downstream molecules that receive signals from GPCRs and trigger cell responses. The GPCR-G protein selectivity mechanism on how they properly and timely interact is still unclear. Read More

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Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil.

Sci Rep 2021 Apr 12;11(1):7936. Epub 2021 Apr 12.

Instituto de Microbiologia Paulo de Goes, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.

Optochin susceptibility testing is a major assay used for presumptive identification of Streptococcus pneumoniae. Still, atypical optochin-resistant (Opt) pneumococci have been reported and this phenotype has been attributed to nucleotide substitutions in the genes coding for the FFATPase. While substitutions in the atpC gene (c-subunit of ATPase) are more common and better characterized, data on mutations in the atpA (a-subunit) are still limited. Read More

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An ABCC-type transporter endowing glyphosate resistance in plants.

Proc Natl Acad Sci U S A 2021 Apr;118(16)

Australian Herbicide Resistance Initiative, School of Agriculture and Environment, University of Western Australia, WA 6009, Australia;

Glyphosate is the most widely used herbicide in world agriculture and for general vegetation control in a wide range of situations. Global and often intensive glyphosate selection of very large weedy plant populations has resulted in widespread glyphosate resistance evolution in populations of many weed species. Here, working with a glyphosate-resistant (GR) population that evolved in a Western Australia agricultural field, we identified an ATP-binding cassette (ABC) transporter () that is consistently up-regulated in GR plants. Read More

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Identification of conserved Mycoplasma agalactiae surface antigens by immunoproteomics.

Vet Immunol Immunopathol 2021 Apr 7;236:110239. Epub 2021 Apr 7.

Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Italy; Mediterranean Center for Disease Control (MCDC), Università degli studi di Sassari, Italy. Electronic address:

Contagious agalactia represents one of the most relevant infectious diseases of dairy sheep, with Mycoplasma agalactiae being the primary etiological agent. The early, sensitive, and specific identification of infected animals, as well as the development of efficient prophylactic tools, remain challenging. Here, we present a comprehensive characterization of M. Read More

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Molecular characterization of a novel p38 MAPK cDNA from Cyclina sinensis and its potential immune-related function under the threat of Vibrio anguillarum.

Comp Biochem Physiol B Biochem Mol Biol 2021 Apr 9:110599. Epub 2021 Apr 9.

College of Life Sciences, Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin, China. Electronic address:

The p38 mitogen-activated protein kinase (MAPK) is one important member of MAPK family and reported to serve a predominant function in regulating innate immunity after the occurrence of certain infection. In the present study, one novel p38 MAPK gene was acquired from Cyclina sinensis based on the RNA-seq analysis and designated as Csp38 MAPK. This novel gene contained a full length of 1781 bp, 1104 bp of which was deemed as open reading frames and gave rise to a peptide of 367 amino acids with a predicted molecular weight of 42. Read More

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Cross-reactivity of two human IL-6 family cytokines OSM and LIF explored by protein-protein docking and molecular dynamics simulation.

Biochim Biophys Acta Gen Subj 2021 Apr 9:129907. Epub 2021 Apr 9.

School of Pharmaceutical Sciences, Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Chongqing University, Chongqing 401331, China. Electronic address:

Background: Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are two important pro-inflammatory cytokines of the interleukin-6 (IL-6) family. The two cytokines mediated signaling was recently found to be closely associated with cancer and chronic inflammation, which represent promising therapeutic targets for the treatment of many solid tumors and inflammatory disease. As the most closely related members, cross-reactivity of them may result in undesired activation of off-target cells, leading to toxicity or lack of efficacy of the therapeutic effects. Read More

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Phosphorylation of the LIR domain of SCOC modulates ATG8 binding affinity and specificity.

J Mol Biol 2021 Apr 9:166987. Epub 2021 Apr 9.

Molecular Cell Biology of Autophagy, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK; current address: Department of Molecular Cell Biology, Centre for Cancer Biomedicine, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0379 Oslo, Norway. Electronic address:

Autophagy is a highly conserved degradative pathway, essential for cellular homeostasis and implicated in diseases including cancer and neurodegeneration. Autophagy-related 8 (ATG8) proteins play a central role in autophagosome formation and selective delivery of cytoplasmic cargo to lysosomes by recruiting autophagy adaptors and receptors. The LC3-interacting region (LIR) docking site (LDS) of ATG8 proteins binds to LIR motifs present in autophagy adaptors and receptors. Read More

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Mutation of P-element somatic inhibitor induces male sterility in the diamondback moth, Plutella xylostella.

Pest Manag Sci 2021 Apr 11. Epub 2021 Apr 11.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences/Institute of Plant Physiology and Ecology, Shanghai, 200032, China.

Background: Genetic manipulation of sex determination pathways in insects provides the basis for a broad range of strategies to benefit agricultural security and human health. The P-element somatic inhibitor (PSI) protein, an exon splicing silencer that promotes male-specific splicing of dsx, plays a critical role in male sexual differentiation and development. The functions of PSI have been characterized in the lepidopteran model species Bombyx mori. Read More

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A multilayered cross-species analysis of GRAS transcription factors uncovered their functional networks in plant adaptation to the environment.

J Adv Res 2021 Mar 29;29:191-205. Epub 2020 Oct 29.

Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

Introduction: Environmental stress is both a major force of natural selection and a prime factor affecting crop qualities and yields. The impact of the GRAS [gibberellic acid-insensitive (GAI), repressor of GA1-3 mutant (RGA), and scarecrow (SCR)] family on plant development and the potential to resist environmental stress needs much emphasis.

Objectives: This study aims to investigate the evolution, expansion, and adaptive mechanisms of of important representative plants during polyploidization. Read More

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Identification of PCBP1 as a Novel Modulator of Mammalian Circadian Clock.

Front Genet 2021 26;12:656571. Epub 2021 Mar 26.

Hubei Engineering Research Center of Special Wild Vegetables Breeding and Comprehensive Utilization Technology, Hubei Normal University, Huangshi, China.

The circadian clock governs our daily cycle of behavior and physiology. Previous studies have identified a handful of core clock components and hundreds of circadian modifiers. Here, we report the discovery that poly(C)-binding protein 1 (PCBP1), displaying a circadian expression pattern, was a novel circadian clock regulator. Read More

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A Novel Autoantibody Induced by Bacterial Biofilm Conserved Components Aggravates Lupus Nephritis.

Front Immunol 2021 25;12:656090. Epub 2021 Mar 25.

Beijing Institute of Pharmacology and Toxicology, Beijing, China.

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease with multiple autoantibody production and often affects the kidneys, known as lupus nephritis. However, the mechanism underlying lupus nephritis development is unclear. Biofilms that protect bacteria from stress are ubiquitous in almost every environment. Read More

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Damage-Induced Calcium Signaling and Reactive Oxygen Species Mediate Macrophage Activation in Zebrafish.

Front Immunol 2021 26;12:636585. Epub 2021 Mar 26.

LPHI, Univ Montpellier, CNRS, Montpellier, France.

Immediately after a wound, macrophages are activated and change their phenotypes in reaction to danger signals released from the damaged tissues. The cues that contribute to macrophage activation after wounding are still poorly understood. Calcium signaling and Reactive Oxygen Species (ROS), mainly hydrogen peroxide, are conserved early wound signals that emanate from the wound and guide neutrophils within tissues up to the wound. Read More

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Clinical Variability of -Associated Early-Onset Parkinsonism.

Front Neurol 2021 25;12:648457. Epub 2021 Mar 25.

Sorbonne Université, Institut du Cerveau-Paris Brain Institute-ICM, INSERM, CNRS, Assistance Publique Hôpitaux de Paris, Hôpital Pitié-Salpêtrière, CIC Neurosciences, Paris, France.

Autosomal recessive early-onset parkinsonism is clinically and genetically heterogeneous. Mutations of three genes, , and cause pure phenotypes usually characterized by levodopa-responsive Parkinson's disease. By contrast, mutations of other genes, including , and , cause rarer, more severe diseases with a poor response to levodopa, generally with additional atypical features. Read More

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The Role of Autophagy in Skeletal Muscle Diseases.

Front Physiol 2021 25;12:638983. Epub 2021 Mar 25.

Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, China.

Skeletal muscle is the most abundant type of tissue in human body, being involved in diverse activities and maintaining a finely tuned metabolic balance. Autophagy, characterized by the autophagosome-lysosome system with the involvement of evolutionarily conserved autophagy-related genes, is an important catabolic process and plays an essential role in energy generation and consumption, as well as substance turnover processes in skeletal muscles. Autophagy in skeletal muscles is finely tuned under the tight regulation of diverse signaling pathways, and the autophagy pathway has cross-talk with other pathways to form feedback loops under physiological conditions and metabolic stress. Read More

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The microRNA miR-33 is a pleiotropic regulator of metabolic and developmental processes in D. melanogaster.

Dev Dyn 2021 Apr 11. Epub 2021 Apr 11.

Department of Biology and Program in Biochemistry, Vassar College, Poughkeepsie, NY, USA.

Background: miR-33 family members are well characterized regulators of cellular lipid levels in mammals. Previous studies have shown that overexpression of miR-33 in Drosophila melanogaster leads to elevated triacylglycerol (TAG) levels in certain contexts. Although loss of miR-33 in flies causes subtle defects in larval and adult ovaries, the effects of miR-33 deficiency on lipid metabolism and other phenotypes impacted by metabolic state have not yet been characterized. Read More

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Clinical and molecular features of two diabetes families carrying mitochondrial ND1 T3394C mutation.

Ir J Med Sci 2021 Apr 11. Epub 2021 Apr 11.

Department of Obstetrics and Gynecology, South Hospital of Fujian Provincial Hospital, Fuzhou, 350007, China.

Background: Mutations in mitochondrial DNA (mtDNA) are found to be associated with type 2 diabetes mellitus (T2DM). However, the molecular pathogenesis of these mutations in T2DM is still poorly understood.

Methods: In this study, we report here the molecular features of two Han Chinese families with maternally transmitted T2DM. Read More

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Major components of the KARRIKIN INSENSITIVE2-dependent signaling pathway are conserved in the liverwort Marchantia polymorpha.

Plant Cell 2021 Apr 11. Epub 2021 Apr 11.

Tohoku University, Graduate School of Life Sciences, Aoba-ku, Sendai, 980-8577, Japan.

KARRIKIN INSENSITIVE2 (KAI2) was first identified as a receptor of karrikins, smoke-derived germination stimulants. KAI2 is also considered a receptor of an unidentified endogenous molecule called the KAI2-ligand (KL). Upon KAI2 activation, signals are transmitted through degradation of D53/SMXL proteins via MAX2-dependent ubiquitination. Read More

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