5 results match your criteria icmk

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Expression, purification and characterization of the IcmG and IcmK proteins of the type IVB secretion system from Coxiella burnetii.

Protein Expr Purif 2021 Oct 11;186:105905. Epub 2021 May 11.

Division of Biochemistry, Department of Chemistry, University of Crete, GR-71003, Voutes, Greece. Electronic address:

Coxiella burnetii, the causative agent of Q fever, is an intracellular bacterial pathogen. Studies on Coxiella have shown that a type IVB secretion system (T4BSS) contributes to the establishment of the infection by transferring protein molecules. In this report, we focus on two core proteins of the Coxiella T4BSS, namely the IcmG/DotF protein (CBU_1626) and the IcmK/DotH protein (CBU_1628). Read More

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October 2021

Molecular typing of Legionella pneumophila isolates from environmental water samples and clinical samples using a five-gene sequence typing and standard Sequence-Based Typing.

PLoS One 2018 1;13(2):e0190986. Epub 2018 Feb 1.

Guangzhou KingMed Center for Clinical Laboratory, Guangzhou, China.

Inadequate discriminatory power to distinguish between L. pneumophila isolates, especially those belonging to disease-related prevalent sequence types (STs) such as ST1, ST36 and ST47, is an issue of SBT scheme. In this study, we developed a multilocus sequence typing (MLST) scheme based on two non-virulence loci (trpA, cca) and three virulence loci (icmK, lspE, lssD), to genotype 110 L. Read More

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Molecular evolution of virulence genes and non-virulence genes in clinical, natural and artificial environmental isolates.

PeerJ 2017 4;5:e4114. Epub 2017 Dec 4.

Guangzhou KingMed Center for Clinical Laboratory, Guangzhou, China.

Background: is the main causative agent of Legionnaires' disease. Free-living amoeba in natural aquatic environments is the reservoir and shelter for From natural water sources, can colonize artificial environments such as cooling towers and hot-water systems, and then spread in aerosols, infecting the susceptible person. Therefore, molecular phylogeny and genetic variability of from different sources (natural water, artificial water, and human lung tissue) might be distinct because of the selection pressure in different environments. Read More

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December 2017

Evidence of the presence of a functional Dot/Icm type IV-B secretion system in the fish bacterial pathogen Piscirickettsia salmonis.

PLoS One 2013 28;8(1):e54934. Epub 2013 Jan 28.

Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.

Piscirickettsia salmonis is a fish bacterial pathogen that has severely challenged the sustainability of the Chilean salmon industry since its appearance in 1989. As this Gram-negative bacterium has been poorly characterized, relevant aspects of its life cycle, virulence and pathogenesis must be identified in order to properly design prophylactic procedures. This report provides evidence of the functional presence in P. Read More

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Comparative sequence analysis of the icm/dot genes in Legionella.

Plasmid 2004 Mar;51(2):127-47

Columbia Genome Center, Columbia University College of Physicians and Surgeons, 1150 St. Nicholas Avenue, New York, NY 10032, USA.

The icm/dot genes in Legionella pneumophila are essential for the ability of the bacteria to survive within macrophages in lung infections such as Legionnaires' disease, or amoebae in nature. The 22 genes of the complex, thought to encode a transport apparatus for transfer of effector molecules into the host cell cytoplasm, are located in two chromosomal loci. We demonstrate that these genes are present in all the L. Read More

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