Methods 2021 Apr 7. Epub 2021 Apr 7.
Montreal Heart Institute, 5000 Belanger, Montréal, Québec, H1T 1C8, Canada; Departments of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, Québec, H3C 3J7, Canada; Pharmacology and Physiology, Université de Montréal, Montréal, Québec, H3C 3J7, Canada; Université de Montréal, Montréal, Québec, H3C 3J7, Canada. Electronic address:
Cardiac fibroblasts play a critical role in extracellular matrix homeostasis, wound healing, and cardiac interstitial fibrosis: the latter being a pathophysiological response to a chronic increase in afterload. Using a standard protocol to isolate cardiac fibroblasts and maintain them in their quiescent phenotype in vitro will enable a better understanding of cardiac fibroblast biology and their role in the response to profibrotic stimuli. Here, we describe an enzymatic method for isolating cardiac fibroblasts. Read More