iScience 2021 Mar 20;24(3):102219. Epub 2021 Feb 20.
BGI-Qingdao, BGI-Shenzhen, Qingdao, Shandong 266555, China.
In this study, six bacterial isolates with variable GC, including as mesophilic reference strain, were selected to compare hybrid assembly strategies based on next-generation sequencing (NGS) of short reads, single-tube long-fragment reads (stLFR) sequencing, and Oxford Nanopore Technologies (ONT) sequencing platforms. We obtained the complete genomes using the hybrid assembler Unicycler based on the NGS and ONT reads; others were assembled using NGS, stLFR, and ONT reads by using different strategies. The contiguity, accuracy, completeness, sequencing costs, and DNA material requirements of the investigated strategies were compared systematically. Read More