2,131 results match your criteria hyaluronidase activity


Human Hyaluronidase PH20 Potentiates the Antitumor Activities of Mesothelin-Specific CAR-T Cells Against Gastric Cancer.

Front Immunol 2021 13;12:660488. Epub 2021 Jul 13.

Center for Cell Regeneration and Biotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

T cell infiltration into tumors is essential for successful immunotherapy against solid tumors. Herein, we found that the expression of hyaluronic acid synthases (HAS) was negatively correlated with patient survival in multiple types of solid tumors including gastric cancer. HA impeded anti-tumor activities of anti-mesothelin (MSLN) chimeric antigen receptor T cells (CAR-T cells) against gastric cancer cells by restricting CAR-T cell mobility . Read More

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Synthesis and biological evaluations of oleanolic acid indole derivatives as hyaluronidase inhibitors with enhanced skin permeability.

J Enzyme Inhib Med Chem 2021 Dec;36(1):1665-1678

School of Biotechnology and Health Sciences, International Healthcare Innovation Institute (Jiangmen), Wuyi University, Jiangmen, China.

Oleanolic acid (OA) is a natural cosmeceutical compound with various skin beneficial activities including inhibitory effect on hyaluronidase but the anti-hyaluronidase activity and mechanisms of action of its synthetic analogues remain unclear. Herein, a series of OA derivatives were synthesised and evaluated for their inhibitory effects on hyaluronidase. Compared to OA, an induction of fluorinated () and chlorinated () indole moieties led to enhanced anti-hyaluronidase activity (IC = 80. Read More

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December 2021

3D structure prediction, dynamic investigation and rational construction of an epitope-masked thermostable bovine hyaluronidase.

Int J Biol Macromol 2021 Jul 21;187:544-553. Epub 2021 Jul 21.

Department of Biochemistry, Abadan University of Medical Sciences, Abadan, Iran. Electronic address:

Hyaluronidase (HAase) from bovine testes (BTH) has long been used in broad pharmaceutical areas, while it is associated with drawbacks in aspects of solubility, immunogenicity and pharmacokinetics. These issues can be addressed by gaining structural insights and designing rational modifications to the enzyme structure, as proposed in this study. A 3D structural model was built for HAase and underwent 40 ns of molecular dynamic simulation to examine its thermostability under normal, melting, and extreme conditions. Read More

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Retrobulbar Hyaluronidase Injection: Does It Reach the Eye?

Ophthalmic Plast Reconstr Surg 2021 Jul 21. Epub 2021 Jul 21.

Jhaveri Microbiology Centre, Brien Holden Eye Research Centre Ophthalmic Plastic Surgery Service, L.V. Prasad Eye Institute, Hyderabad, India.

Purpose: To determine if retrobulbar injection of hyaluronidase reaches the vitreous cavity, and to determine its concentration in the vitreous.

Methods: Prospective case-control study. Patients undergoing evisceration with implant for noninfective blind eyes were enrolled in the study. Read More

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Dispersive effects and focused biodistribution of recombinant human hyaluronidase PH20: A locally acting and transiently active permeation enhancer.

PLoS One 2021 22;16(7):e0254765. Epub 2021 Jul 22.

Halozyme Therapeutics, Inc., San Diego, CA, United States of America.

Background: Recombinant human hyaluronidase PH20 (rHuPH20) facilitates the dispersion and absorption of subcutaneously administered therapeutic agents. This study aimed to characterize the transient, local action of rHuPH20 in the subcutaneous (SC) space using focused biodistribution and dye dispersion studies conducted in mice.

Materials And Methods: To evaluate the biodistribution of rHuPH20, mice were intradermally administered rHuPH20 (80 U). Read More

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CD44-Targeted and Enzyme-Responsive Photo-Cross-Linked Nanogels with Enhanced Stability for In Vivo Protein Delivery.

Biomacromolecules 2021 Jul 21. Epub 2021 Jul 21.

College of Materials Science and Engineering, Jilin Institute of Chemical Technology, Jilin City 132022, P. R. China.

One of the biggest challenges of the protein delivery system is to realize stable and high protein encapsulation efficiency in blood circulation and rapid release of protein in the targeted tumor cells. To overcome these hurdles, we fabricated enzyme-responsive photo-cross-linked nanogels (EPNGs) through UV-triggered chemical cross-linking of cinnamyloxy groups in the side chain of PEGylation hyaluronic acid (HA) for CD44-targeted transport of cytochrome (CC). The EPNGs showed high loading efficiency and excellent stability in different biological media. Read More

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Nanoparticle-driven self-assembling injectable hydrogels provide a multi-factorial approach for chronic wound treatment.

Acta Biomater 2021 Jul 13. Epub 2021 Jul 13.

Group of Molecular and Industrial Biotechnology, Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Rambla Sant Nebridi, 22, 08222, Terrassa, Spain. Electronic address:

Chronic wounds represent a major health burden and drain on medical system. Efficient wound repair is only possible if the dressing materials target simultaneously multiple factors involved in wound chronicity, such as deleterious proteolytic and oxidative enzymes and high bacterial load. Here we develop multifunctional hydrogels for chronic wound management through self-assembling of thiolated hyaluronic acid (HA-SH) and bioactive silver-lignin nanoparticles ([email protected] NPs). Read More

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Overexpression of KIAA1199, a novel strong hyaluronidase, is a poor prognostic factor in patients with osteosarcoma.

J Orthop Surg Res 2021 Jul 7;16(1):439. Epub 2021 Jul 7.

Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi, 466-8550, Japan.

Background: Hyaluronan (HA) has been shown to play important roles in the growth, invasion, and metastasis of malignant tumors. KIAA1199, which has potent HA-degrading activity, has been reported to be expressed in various malignancies and associated with patient prognosis. However, there are no reports on the expression of KIAA1199 in osteosarcoma. Read More

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Analysis of High Molecular Mass Compounds from the Spider Venom Gland. A Transcriptomic and MS ID Approach.

Toxins (Basel) 2021 06 29;13(7). Epub 2021 Jun 29.

Grupo de Génetica, Regeneración y Cáncer, Universidad de Antioquia UdeA, Carrera 53 No. 61-30, Medellín 050010, Colombia.

Nowadays, spider venom research focuses on the neurotoxic activity of small peptides. In this study, we investigated high-molecular-mass compounds that have either enzymatic activity or housekeeping functions present in either the venom gland or venom of . We used proteomic and transcriptomic-assisted approaches to recognize the proteins sequences related to high-molecular-mass compounds present in either venom gland or venom. Read More

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Flavonoid and Phenolic Acids Content and In Vitro Study of the Potential Anti-Aging Properties of (Miq.) Koidz Cultivated in Wasabi Farm Poland.

Int J Mol Sci 2021 Jun 9;22(12). Epub 2021 Jun 9.

Chair and Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, 8 Jaczewskiego Str., 20-090 Lublin, Poland.

Skin aging is a natural, unavoidable, and complex process caused by oxidative stress. As a consequence, it leads to an increase in the activation of extracellular matrix disruption enzymes and DNA damage. The search for natural sources that inhibit these mechanisms can be a good approach to prevent skin aging. Read More

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Evaluating the Antioxidants, Whitening and Antiaging Properties of Rice Protein Hydrolysates.

Molecules 2021 Jun 12;26(12). Epub 2021 Jun 12.

Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402204, Taiwan.

Plant-derived protein hydrolysates have potential applications in nutrition. Rice protein hydrolysates (RPHs), an excellent source of proteins, have attracted attention for the development of cosmeceuticals. However, few studies have reported the potential application of RPH in analysis, and this study examined their antioxidant activities and the inhibitory activities of skin aging enzymes. Read More

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Comparative study on the interaction between flavonoids with different core structures and hyaluronidase.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Dec 11;262:120079. Epub 2021 Jun 11.

Department of Medical Chemistry, Key Laboratory of Medical Molecular Probes, School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.

Hyaluronidase (HAase) is an important enzyme involved in a promoting inflammation pathway. Flavonoids are a group of major polyphenols including flavonols (such as myricetin and rutin), dihydroflavones (such as naringin and hesperidin), and isoflavones (such as genistein and puerarin), which have been proved to possess anti-inflammatory effects. In this study, the binding of the six flavonoids to HAase was investigated by steady state and time-resolved fluorescence, circular dichroism (CD) spectroscopy and molecular docking methods. Read More

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December 2021

The effects of cryopreservation on the acrosome structure, enzyme activity, motility, and fertility of bovine, ovine, and goat sperm.

Anim Reprod 2021 Mar 8;17(4):e20200219. Epub 2021 Mar 8.

College of Life Science and The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, P.R. China.

The study was designed to investigate the effects of cryopreservation on bovine, ovine, and goat sperm motility, acrosome structure, enzyme activity, and fertilization ability. Percentage of sperm with hyaluronidase enzyme (HYD) activity was detected by a modified sodium hyaluronate-gelatin membrane. The N-α-benzoyl-DL-arginine-p-nitroanilide (BNPNA) method was used to assess the sperm acrosome enzyme (ACE). Read More

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Anti-Ageing Potential of Heldr. Phenolics.

Molecules 2021 May 25;26(11). Epub 2021 May 25.

Department of Pharmacognosy & Chemistry of Natural Products, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.

In recent years, the use of species as bioactive agents is increasing exponentially. The present study aimed to investigate the chemical constituents, as well as the anti-ageing potential of the cultivated Heldr. The chemical fingerprinting of the ethyl acetate residue of this plant was studied using 1D and 2D-NMR spectra. Read More

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Hyaluronidase-responsive phototheranostic nanoagents for fluorescence imaging and photothermal/photodynamic therapy of methicillin-resistant infections.

Biomater Sci 2021 Jun;9(12):4484-4495

State Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

Infectious diseases associated with antibiotic-resistant bacteria are ever-growing threats to public health. Effective treatment and detection methods of bacterial infections are in urgent demand. Herein, novel phototheranostic nanoagents ([email protected] nanosheets, MHC NSs) with hyaluronidase (HAase)-responsive fluorescence imaging (FLI) and photothermal/photodynamic therapy (PTT/PDT) functions were prepared. Read More

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Herbal Extracts as Potential Antioxidant, Anti-Aging, Anti-Inflammatory, and Whitening Cosmeceutical Ingredients.

Chem Biodivers 2021 Jul 7;18(7):e2100245. Epub 2021 Jun 7.

Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Huachiew Chalermprakiet University, Samutprakarn, 10250, Thailand.

The aim of this research was to investigate and compare the antioxidant, anti-tyrosinase, anti-aging, and anti-inflammatory activities of 16 herbal extracts for topical application in cosmetic/cosmeceutical products. Herbal plant materials were extracted by infusion in boiled water for 15 min. The total phenolic content and total flavonoid content of each extract were investigated by the Folin-Ciocalteu and aluminum chloride methods, respectively. Read More

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Phytochemical composition, antisnake venom and antibacterial activities of ethanolic extract of Aegiphila integrifolia (Jacq) Moldenke leaves.

Toxicon 2021 Jul 11;198:121-131. Epub 2021 May 11.

Chemical Engineering Section, Military Institute of Engineering, Rio de Janeiro, RJ, Brazil. Electronic address:

Snakebites are considered a major neglected tropical disease, resulting in around 100,000 deaths per year. The recommended treatment by the WHO is serotherapy, which has limited effectiveness against the toxins involved in local tissue damage. In some countries, patients use plants from folk medicines as antivenoms. Read More

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Screening of Antioxidative Properties and Inhibition of Inflammation-Linked Enzymes by Aqueous and Ethanolic Extracts of Plants Traditionally Used in Wound Healing in Poland.

Antioxidants (Basel) 2021 Apr 28;10(5). Epub 2021 Apr 28.

Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw Medical University, Banacha 1, 02-097 Warsaw, Poland.

A wide range of plant-derived preparations have been used against skin inflammatory disorders and as wound healing agents in traditional medicine. The purpose of the study was to determine the antioxidant activity of aqueous and 70% ethanolic extracts from eleven species of plants traditionally used in Poland to treat inflammatory skin diseases. The ability of extracts to scavenge 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), hydrogen peroxide (HO), and superoxide anion (O), was studied. Read More

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Combinations of Piperine with Hydroxypropyl-β-Cyclodextrin as a Multifunctional System.

Int J Mol Sci 2021 Apr 18;22(8). Epub 2021 Apr 18.

Department of Pharmacognosy, Faculty of Pharmacy, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznań, Poland.

Piperine is an alkaloid that has extensive pharmacological activity and impacts other active substances bioavailability due to inhibition of CYP450 enzymes, stimulation of amino acid transporters and -glycoprotein inhibition. Low solubility and the associated low bioavailability of piperine limit its potential. The combination of piperine with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) causes a significant increase in its solubility and, consequently, an increase in permeability through gastrointestinal tract membranes and the blood-brain barrier. Read More

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Permeability of Extract Component-Physodic Acid through the Blood-Brain Barrier as an Important Argument for Its Anticancer and Neuroprotective Activity within the Central Nervous System.

Cancers (Basel) 2021 Apr 5;13(7). Epub 2021 Apr 5.

Department of Pharmacognosy, Poznan University of Medical Sciences, Święcicki 4 Str, 60-781 Poznań, Poland.

Lichen secondary metabolites are characterized by huge pharmacological potential. Our research focused on assessing the anticancer and neuroprotective activity of acetone extract (HP extract) and physodic acid, its major component. The antitumor properties were evaluated by cytotoxicity analysis using A-172, T98G, and U-138 MG glioblastoma cell lines and by hyaluronidase and cyclooxygenase-2 (COX-2) inhibition. Read More

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Effects of silencing epididymal vascular endothelial growth factor (VEGF) expression on hyaluronidase (HYD) activity in arsenic poisoning rats through downregulating VEGF receptor 2 (VEGFR2).

Bioengineered 2021 12;12(1):1351-1359

Department of Pathology and Pathophysiology, College of Basic Medical Science, Guizhou Medical University, Guiyang, Guizhou, China.

RNA interference (RNAi) was used to investigate the role of epididymal vascular endothelial growth factor (VEGF) gene expression on sperm hyaluronidase (HYD) in a rat model of arsenic poisoning and to identify a new gene therapy target for male infertility caused by arsenic poisoning. The Rat model of chronic arsenic poisoning was established. And we found that positive expression of VEGF and VEGF receptor 2 (VEGFR2) was observed by Immunohistochemical staining in the epididymal tissues of arsenic-exposed rats. Read More

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December 2021

Nutricosmetic effects of Asparagus officinalis: a potent matrix metalloproteinase-1 inhibitor.

Sci Rep 2021 Apr 22;11(1):8772. Epub 2021 Apr 22.

Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand.

This study aimed to investigate the nutricosmetic effect of Asparagus officinalis extracts. The tip and spear of A. officinalis were successively extracted with 95% ethanol. Read More

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Self-assembled nanomedicine combining a berberine derivative and doxorubicin for enhanced antitumor and antimetastatic efficacy via mitochondrial pathways.

Nanoscale 2021 Apr 29;13(13):6605-6623. Epub 2021 Mar 29.

School of Pharmaceutical Sciences, Sun Yat-sen University, University Town, Guangzhou 510006, P. R. China.

Mitochondria play a central role in cancer progression and tumor metastasis, and nanomedicines targeting mitochondria have emerged as a promising strategy for tumor therapy. However, mitochondria targeting strategies have not been widely explored in the inhibition of tumor metastasis, and they have disadvantages of complicated preparation, low drug loading, systemic toxicity of the carriers and poor accumulation at tumor sites. Here we firstly developed self-assembled nanodrugs with a high drug loading (∼68%) comprised of a berberine derivative (Ber) and doxorubicin (Dox) by a simple nano-precipitation method, which successfully altered the target location of Dox from the nucleus to mitochondria and therefore inhibited the proliferation, invasion and migration of MDA-MB-231 cells by triggering cell apoptosis. Read More

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Abundance and size of hyaluronan in naked mole-rat tissues and plasma.

Sci Rep 2021 Apr 12;11(1):7951. Epub 2021 Apr 12.

Molecular Physiology Research Unit (URPhyM), NARILIS, University of Namur, Namur, Belgium.

Large amounts of ultra-high molecular weight hyaluronan (HA) have been described as the main cause of cancer resistance in naked mole-rats (Heterocephalus glaber, NMR). Our work examined HA metabolism in these rodents more closely. HA was localized and quantified using HA binding proteins. Read More

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Geraniol exerts its antiproliferative action by modulating molecular targets in lung and skin carcinoma cells.

Phytother Res 2021 Jul 7;35(7):3861-3874. Epub 2021 Apr 7.

Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.

Geraniol, an acyclic monoterpene present in several plant species' essential oils, is utilized as a food additive. It possesses potent antiproliferative and antitumor effects ascribed to its antiinflammatory, and antioxidant properties. The study aimed to understand geraniol's mechanism in human lung and skin cancer cells by employing molecular and cell target-based assays. Read More

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Antihyaluronidase and Alkaline Phosphatase (ALP) Activities of Medicinal Plants to Combat Venom-Induced Toxicities.

Biomed Res Int 2021 16;2021:6618349. Epub 2021 Mar 16.

Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, 22060 KPK, Pakistan.

Snakebite is one of the most neglected diseases of developing countries. Deaths due to snakebite envenoming are quite high in Pakistan, and many deaths are caused by envenomation. Traditional use of medicinal plants against snakebites is a common practice in Pakistan due to countless benefits. Read More

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Supramolecular Structuring of Hyaluronan-Lactose-Modified Chitosan Matrix: Towards High-Performance Biopolymers with Excellent Biodegradation.

Biomolecules 2021 03 5;11(3). Epub 2021 Mar 5.

Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Carbohydrate Science Department, 20133 Milan, Italy.

Non-covalent interactions in supramolecular chemistry provide useful systems to understand biological processes, and self-assembly systems are suitable assets to build-up innovative products for biomedical applications. In this field, polyelectrolyte complexes are interesting, especially when polysaccharides are involved, due to their non-toxicity and bio-absorbability. In this work, we investigated a polyelectrolyte formed by hyaluronic acid (HA), a negatively charged linear polysaccharide, with Chitlac (Ch), a positively charged lactose-modified chitosan. Read More

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Chemical Profiles, Anticancer, and Anti-Aging Activities of Essential Oils of (L.) DC. and L.

Plants (Basel) 2021 Mar 31;10(4). Epub 2021 Mar 31.

Chemistry of Natural Compounds Department, National Research Center, 33 El Bohouth St., Dokki, Giza 12622, Egypt.

Plants belonging to the Asteraceae family are widely used as traditional medicinal herbs around the world for the treatment of numerous diseases. In this work, the chemical profiles of essential oils (EOs) of the above-ground parts of (L.) DC. Read More

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Effect of Liposome-Encapsulated Dextrazide on Local Regulation of Extracellular Matrix Metabolism in Liver and Lungs of Mice with during Chronic BCG-Induced Granulomatosis.

Bull Exp Biol Med 2021 Mar 31;170(5):640-644. Epub 2021 Mar 31.

Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

The liposomal form of isonicotinic acid hydrazide conjugate with oxidized dextran (liposomeencapsulated dextrazide, LEDZ) injected intraperitoneally for 3 months to BALB/c mice with chronic BCG-induced granulomatosis (6 month after infection) down-regulated activities of protease (MMP) and antiprotease (TIMP-1, TIMP-2, α2-macroglobulin) components of the regulation system, which indicates a drop of destructive and inflammatory potential of BCGinduced granulomatosis. The persistent enhanced hyaluronidase activity in the liver and its reduced (normalized to control level) activity in the lungs after administration of LEDZ indicates a greater hydrolysis of hyaluronan in the liver than in the lungs. LEDZ-induced changes in MMP/TIMP system in mouse liver and lungs are characterized with relative elevation of protease activity over that of antiprotease one. Read More

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Recombinant human hyaluronidase PH20-mediated dermal spreading activity in mice is not altered by steroids, antihistamines, or salicylic acid.

Drug Metab Pers Ther 2020 Dec 25. Epub 2020 Dec 25.

Halozyme Therapeutics Inc., San Diego, CA, USA.

Objectives: Drug-drug interaction studies for hyaluronidase safety assessments have evaluated only animal-derived enzyme preparations. We therefore set out to evaluate whether high-dose administration of two antihistamines, a potent corticosteroid, steroid hormone, adrenocorticotropic hormone (ACTH), or salicylic acid would alter the dispersive activity of recombinant human hyaluronidase PH20 (rHuPH20).

Methods: NCr nu/nu mice were pretreated with diphenhydramine, cetirizine, dexamethasone, estrogen, ACTH, salicylic acid, and/or neutral-buffered saline (NBS). Read More

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December 2020