34 results match your criteria hyaluronic acid-gelatin


Surface Coating with Hyaluronic Acid-Gelatin-Crosslinked Hydrogel on Gelatin-Conjugated Poly(dimethylsiloxane) for Implantable Medical Device-Induced Fibrosis.

Pharmaceutics 2021 Feb 17;13(2). Epub 2021 Feb 17.

Department of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.

Polydimethylsiloxane (PDMS) is a biocompatible polymer that has been applied in many fields. However, the surface hydrophobicity of PDMS can limit successful implementation, and this must be reduced by surface modification to improve biocompatibility. In this study, we modified the PDMS surface with a hydrogel and investigated the effect of this on hydrophilicity, bacterial adhesion, cell viability, immune response, and biocompatibility of PDMS. Read More

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February 2021

Carboxymethyl chitosan microspheres loaded hyaluronic acid/gelatin hydrogels for controlled drug delivery and the treatment of inflammatory bowel disease.

Int J Biol Macromol 2021 Jan 18;167:1598-1612. Epub 2020 Nov 18.

School of Pharmaceutical Science, South-Central University for Nationalities, Wuhan 430074, China; National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University for Nationalities, Wuhan 430074, China. Electronic address:

A major drawback of oral treatment of inflammatory bowel disease (IBD) is the non-specific distribution of drugs during long-term treatment. Despite its effectiveness as an anti-inflammatory drug, curcumin (CUR) is limited by its low bioavailability in IBD treatment. Herein, a pH-sensitive composite hyaluronic acid/gelatin (HA/GE) hydrogel drug delivery system containing carboxymethyl chitosan (CC) microspheres loaded with CUR was fabricated for IBD treatment. Read More

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January 2021

Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid - Gelatin injectable hydrogels.

Bioelectrochemistry 2020 Aug 18;134:107536. Epub 2020 Apr 18.

Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Valencia, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valencia, Spain.

Electrical stimulation (ES) has provided enhanced chondrogenesis of mesenchymal stem cells (MSCs) cultured in micro-mass without the addition of exogenous growth factors. In this study, we demonstrate for the first time that ES of MSCs encapsulated in an injectable hyaluronic acid (HA) - gelatin (GEL) mixture enhances the chondrogenic potential of the hydrogel. Samples were stimulated for 21 days with 10 mV/cm at 60 kHz, applied for 30 min every 6 h a day. Read More

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Quantitatively relating brain endothelial cell-cell junction phenotype to global and local barrier properties under varied culture conditions via the Junction Analyzer Program.

Fluids Barriers CNS 2020 Feb 11;17(1):16. Epub 2020 Feb 11.

Fischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, 3110 A. James Clark Hall, College Park, MD, 20742, USA.

Background: The endothelial cell-cell junctions of the blood-brain barrier (BBB) play a pivotal role in the barrier's function. Altered cell-cell junctions can lead to barrier dysfunction and have been implicated in several diseases. Despite this, the driving forces regulating junctional protein presentation remain relatively understudied, largely due to the lack of efficient techniques to quantify their presentation at sites of cell-cell adhesion. Read More

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February 2020

A human co-culture cell model incorporating microglia supports glioblastoma growth and migration, and confers resistance to cytotoxics.

FASEB J 2020 01 5;34(1):1710-1727. Epub 2019 Dec 5.

Brain Tumour Research Centre, Institute of Biological and Biomolecular Sciences (IBBS), School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK.

Despite the importance of the tumor microenvironment in regulating tumor progression, few in vitro models have been developed to understand the effects of non-neoplastic cells and extracellular matrix (ECM) on drug resistance in glioblastoma (GBM) cells. Using CellTrace-labeled human GBM and microglial (MG) cells, we established a 2D co-culture including various ratios of the two cell types. Viability, proliferation, migration, and drug response assays were carried out to assess the role of MG. Read More

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January 2020

Pre-Clinical Drug Testing in 2D and 3D Human In Vitro Models of Glioblastoma Incorporating Non-Neoplastic Astrocytes: Tunneling Nano Tubules and Mitochondrial Transfer Modulates Cell Behavior and Therapeutic Respons.

Int J Mol Sci 2019 Nov 29;20(23). Epub 2019 Nov 29.

Brain Tumour Research Centre, Institute of Biological and Biomedical Sciences (IBBS), School of Pharmacy and Biomedical Sciences, University of Portsmouth, White Swan Road, Portsmouth PO1 2DT, UK.

The role of astrocytes in the glioblastoma (GBM) microenvironment is poorly understood; particularly with regard to cell invasion and drug resistance. To assess this role of astrocytes in GBMs we established an all human 2D co-culture model and a 3D hyaluronic acid-gelatin based hydrogel model (HyStem™-HP) with different ratios of GBM cells to astrocytes. A contact co-culture of fluorescently labelled GBM cells and astrocytes showed that the latter promotes tumour growth and migration of GBM cells. Read More

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November 2019

Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors.

Biomolecules 2019 08 5;9(8). Epub 2019 Aug 5.

Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields. In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering. In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels. Read More

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Modeling the drug release from reduced graphene oxide-reinforced hyaluronic acid/gelatin/poly(ethylene oxide) polymeric films.

Carbohydr Polym 2019 Jul 14;215:189-197. Epub 2019 Mar 14.

Marmara University, Department of Chemical Engineering, 34722, Istanbul, Turkey. Electronic address:

Herein, electroconductive polymeric films consisting of hyaluronic acid (HyA), gelatin (Gel), poly(ethylene oxide) (PEO) reinforced by reduced graphene oxide (RGO) were used in drug release studies to investigate usability of the films as drug carrier in the future. Irbesartan (IRB) used for the treatment of cardiovascular diseases was loaded to the polymeric films and its release kinetic was investigated. Afterwards, the obtained controlled drug release data were simulated using different dynamic differential mathematical models such as 1, 2, 3 degree and Higuchi model. Read More

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In situ injectable hyaluronic acid/gelatin hydrogel for hemorrhage control.

Mater Sci Eng C Mater Biol Appl 2019 May 9;98:628-634. Epub 2019 Jan 9.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.. Electronic address:

Tissue sealants are used for hemorrhage control which is imperative in many surgical procedures. It is a highly challenging task to obtain the ideal tissue sealant. Only a few commercially tissue sealants are available to be used for internal tissue or organ hemorrhage control. Read More

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Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34 cells.

Cell Prolif 2018 Apr 15;51(2):e12407. Epub 2017 Nov 15.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, China.

Objectives: Stem cell factor (SCF) is considered as a commonly indispensable cytokine for proliferation of haematopoietic stem cells (HSCs), which is used in large dosages during ex vivo culture. The work presented here aimed to reduce the consumption of SCF by sustained release but still support cells proliferation and maintain the multipotency of HSCs.

Materials And Methods: Stem cell factor was physically encapsulated within a hyaluronic acid/gelatin double network (HGDN) hydrogel to achieve a slow release rate. Read More

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Differentiation potential of human adipose stem cells bioprinted with hyaluronic acid/gelatin-based bioink through microextrusion and visible light-initiated crosslinking.

Biopolymers 2018 Feb 15;109(2). Epub 2017 Nov 15.

Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka, 560-8531, Japan.

Bioprinting has a great potential to fabricate three-dimensional (3D) functional tissues and organs. In particular, the technique enables fabrication of 3D constructs containing stem cells while maintaining cell proliferation and differentiation abilities, which is believed to be promising in the fields of tissue engineering and regenerative medicine. We aimed to demonstrate the utility of the bioprinting technique to create hydrogel constructs consisting of hyaluronic acid (HA) and gelatin derivatives through irradiation by visible light to fabricate 3D constructs containing human adipose stem cells (hADSCs). Read More

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February 2018

A novel hybrid multichannel biphasic calcium phosphate granule-based composite scaffold for cartilage tissue regeneration.

J Biomater Appl 2018 01 13;32(6):775-787. Epub 2017 Nov 13.

1 Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyoung-Dong, Cheonan, South Korea.

The objective of the present study was to develop a novel hybrid multichannel biphasic calcium phosphate granule (MCG)-based composite system for cartilage regeneration. First, hyaluronic acid-gelatin (HG) hydrogel was coated onto MCG matrix (MCG-HG). Poly(lactic-co-glycolic acid) (PLGA) microspheres was separately prepared and modified with polydopamine subsequent to BMP-7 loading (B). Read More

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January 2018

A hybrid composite system of biphasic calcium phosphate granules loaded with hyaluronic acid-gelatin hydrogel for bone regeneration.

J Biomater Appl 2017 Oct 24;32(4):433-445. Epub 2017 Sep 24.

1 Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Chungnam, Cheonan City, Republic of Korea.

An ideal bone substitute should be made of biocompatible materials that mimic the structure, characteristics, and functions of natural bone. Many researchers have worked on the fabrication of different bone scaffold systems including ceramic-polymer hybrid system. In the present study, we incorporated hyaluronic acid-gelatin hydrogel to micro-channeled biphasic calcium phosphate granules as a carrier to improve cell attachment and proliferation through highly interconnected porous structure. Read More

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October 2017

Higher Ratios of Hyaluronic Acid Enhance Chondrogenic Differentiation of Human MSCs in a Hyaluronic Acid-Gelatin Composite Scaffold.

Materials (Basel) 2016 May 17;9(5). Epub 2016 May 17.

Department of Trauma Surgery, University Medical CentreRegensburg, Franz-Josef-Strauss-Allee 11, Regensburg 93053, Germany.

Mesenchymal stem cells (MSCs) seeded on specific carrier materials are a promising source for the repair of traumatic cartilage injuries. The best supportive carrier material has not yet been determined. As natural components of cartilage's extracellular matrix, hyaluronic acid and collagen are the focus of biomaterial research. Read More

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In Vitro Testing of Scaffolds for Mesenchymal Stem Cell-Based Meniscus Tissue Engineering-Introducing a New Biocompatibility Scoring System.

Materials (Basel) 2016 Apr 7;9(4). Epub 2016 Apr 7.

Department of Trauma Surgery, University Medical Centre Regensburg; Franz Josef Strauss Allee 11, 93053 Regensburg, Germany.

A combination of mesenchymal stem cells (MSCs) and scaffolds seems to be a promising approach for meniscus repair. To facilitate the search for an appropriate scaffold material a reliable and objective testing system is essential. This paper introduces a new scoring for this purpose and analyzes a hyaluronic acid (HA) gelatin composite scaffold and a polyurethane scaffold in combination with MSCs for tissue engineering of meniscus. Read More

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Effect of carbodiimide-derivatized hyaluronic acid gelatin on preventing postsurgical intra-abdominal adhesion formation and promoting healing in a rat model.

J Biomed Mater Res A 2016 May 2;104(5):1175-81. Epub 2016 Feb 2.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.

Adhesions often occur after abdominal surgery. It could cause chronic pelvic pain, intestinal obstruction, and infertility. A hydrogel biomaterial, carbodiimide-derivatized hyaluronic acid gelatin (cd-HA gelatin), has been successfully used to reduce adhesion formation after flexor tendon grafting. Read More

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Investigation of the Viability, Adhesion, and Migration of Human Fibroblasts in a Hyaluronic Acid/Gelatin Microgel-Reinforced Composite Hydrogel for Vocal Fold Tissue Regeneration.

Adv Healthc Mater 2016 Jan 26;5(2):255-65. Epub 2015 Oct 26.

Biomechanics Laboratory, Department of Mechanical Engineering, Faculty of Engineering, McGill University, 817 Rue Sherbrooke Ouest, Montreal, QC, H3A 0C3, Canada.

The potential use of a novel scaffold biomaterial consisting of cross-linked hyaluronic acid (HA)-gelatin (Ge) composite microgels is investigated for use in treating vocal fold injury and scarring. Cell adhesion integrins and kinematics of cell motion are investigated in 2D and 3D culture conditions, respectively. Human vocal fold fibroblast (hVFF) cells are seeded on HA-Ge microgels attached to a HA hydrogel thin film. Read More

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January 2016

Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction.

Biomaterials 2015 Aug 28;61:339-48. Epub 2015 May 28.

Dept. of Cardiology, DH&L, University Medical Center Utrecht, Utrecht, The Netherlands; Interuniversity Cardiology Institute of the Netherlands (ICIN), Utrecht, The Netherlands. Electronic address:

Cardiac cell therapy suffers from limitations related to poor engraftment and significant cell death after transplantation. In this regard, ex vivo tissue engineering is a tool that has been demonstrated to increase cell retention and survival. The aim of our study was to evaluate the therapeutic potential of a 3D-printed patch composed of human cardiac-derived progenitor cells (hCMPCs) in a hyaluronic acid/gelatin (HA/gel) based matrix. Read More

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Viscoelasticity of hyaluronic acid-gelatin hydrogels for vocal fold tissue engineering.

J Biomed Mater Res B Appl Biomater 2016 Feb 27;104(2):283-90. Epub 2015 Feb 27.

Biomechanics Research Laboratory, Department of Mechanical Engineering, McGill University, Montreal, Quebec, H3A 0C3, Canada.

Crosslinked injectable hyaluronic acid (HA)-gelatin (Ge) hydrogels have remarkable viscoelastic and biological properties for vocal fold tissue engineering. Patient-specific tuning of the viscoelastic properties of this injectable biomaterial could improve tissue regeneration. The frequency-dependent viscoelasticity of crosslinked HA-Ge hydrogels was measured as a function of the concentration of HA, Ge, and crosslinker. Read More

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February 2016

Platelet-rich plasma encapsulation in hyaluronic acid/gelatin-BCP hydrogel for growth factor delivery in BCP sponge scaffold for bone regeneration.

J Biomater Appl 2015 Feb 17;29(7):988-1002. Epub 2014 Sep 17.

Department of Regenerative Medicine, Ssangyong-dong, Chungnam, Republic of Korea Instititue of Tissue Regeneration, College of Medicine, Soonchunhyang University, Ssangyong-dong, Chungnam, Republic of Korea

Microporous calcium phosphate based synthetic bone substitutes are used for bone defect healing. Different growth factor loading has been investigated for enhanced bone regeneration. The platelet is a cellular component of blood which naturally contains a pool of necessary growth factors that mediate initiation, continuation, and completion of cellular mechanism of healing. Read More

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February 2015

An in vivo study of composite microgels based on hyaluronic acid and gelatin for the reconstruction of surgically injured rat vocal folds.

J Speech Lang Hear Res 2014 Apr;57(2):S658-73

PURPOSE The objective of this study was to investigate local injection with a hierarchically microstructured hyaluronic acid-gelatin (HA-Ge) hydrogel for the treatment of acute vocal fold injury using a rat model. METHOD Vocal fold stripping was performed unilaterally in 108 Sprague-Dawley rats. A volume of 25 μl saline (placebo controls), HA-bulk, or HA-Ge hydrogel was injected into the lamina propria (LP) 5 days after surgery. Read More

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A combination of biphasic calcium phosphate scaffold with hyaluronic acid-gelatin hydrogel as a new tool for bone regeneration.

Tissue Eng Part A 2014 Jul 21;20(13-14):1993-2004. Epub 2014 Mar 21.

1 Department of Regenerative Medicine, College of Medicine, Soonchunhyang University , Cheonan, South Korea .

A novel bone substitute was fabricated to enhance bone healing by combining ceramic and polymer materials. In this study, Hyaluronic acid (HyA)-Gelatin (Gel) hydrogel was loaded into a biphasic calcium phosphate (BCP) ceramic, and the resulting scaffold, with unique micro- and macroporous orientation, was evaluated for bone regeneration applications. The fabricated scaffold showed high interconnected porosity, with an average compressive strength of 2. Read More

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Production of a composite hyaluronic acid/gelatin blood plasma gel for hydrogel-based adipose tissue engineering applications.

J Biomed Mater Res A 2014 Jul 10;102(7):2220-9. Epub 2013 Aug 10.

Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Izmit Kocaeli, Turkey, 41380.

Standard approaches to soft-tissue reconstruction include autologous adipose tissue transplantation, but most of the transferred adipose tissue is generally reabsorbed in a short time. To overcome this problem, long lasting implantable hydrogel materials that can support tissue regeneration must be produced. The purpose of this study was to evaluate the suitability of composite 3D natural origin scaffolds for reconstructive surgery applications through in vitro tests. Read More

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Synthesis and characterization of hybrid hyaluronic acid-gelatin hydrogels.

Biomacromolecules 2013 Apr 28;14(4):1085-92. Epub 2013 Mar 28.

Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.

Biomimetic hybrid hydrogels have generated broad interest in tissue engineering and regenerative medicine. Hyaluronic acid (HA) and gelatin (hydrolyzed collagen) are naturally derived polymers and biodegradable under physiological conditions. Moreover, collagen and HA are major components of the extracellular matrix (ECM) in most of the tissues (e. Read More

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In situ forming hydrogels composed of oxidized high molecular weight hyaluronic acid and gelatin for nucleus pulposus regeneration.

Acta Biomater 2013 Feb 4;9(2):5181-93. Epub 2012 Oct 4.

Institute of Biomedical Engineering, College of Engineering and College of Medicine, National Taiwan University, Taipei, Taiwan.

Encapsulation of nucleus pulposus (NP) cells within in situ forming hydrogels is a novel biological treatment for early stage intervertebral disc degeneration. The procedure aims to prolong the life of the degenerating discs and to regenerate damaged tissue. In this study we developed an injectable oxidized hyaluronic acid-gelatin-adipic acid dihydrazide (oxi-HAG-ADH) hydrogel. Read More

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February 2013

An injectable nucleus pulposus implant restores compressive range of motion in the ovine disc.

Spine (Phila Pa 1976) 2012 Aug;37(18):E1099-105

Department of Neurological Surgery, University of Pennsylvania, Philadelphia PA 19106, USA.

Study Design: Investigation of injectable nucleus pulposus (NP) implant.

Objective: To assess the ability of a recently developed injectable hydrogel implant to restore nondegenerative disc mechanics through support of NP functional mechanics.

Summary Of Background Data: Although surgical intervention for low back pain is effective for some patients, treated discs undergo altered biomechanics and adjacent levels are at increased risk for accelerated degeneration. Read More

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Characterization of a hierarchical network of hyaluronic acid/gelatin composite for use as a smart injectable biomaterial.

Macromol Biosci 2012 Feb 6;12(2):202-10. Epub 2011 Dec 6.

Department of Mechanical Engineering, McGill University, Montreal, QC, Canada.

Hybrid HA/Ge hydrogel particles are embedded in a secondary HA network to improve their structural integrity. The internal microstructure of the particles is imaged through TEM. CSLM is used to identify the location of the Ge molecules in the microgels. Read More

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February 2012

Biological hydrogel synthesized from hyaluronic acid, gelatin and chondroitin sulfate by click chemistry.

Acta Biomater 2011 Apr 8;7(4):1618-26. Epub 2010 Dec 8.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, People's Republic of China.

In order to mimic the natural cartilage extracellular matrix, which is composed of core proteins and glycosaminoglycans, a biological hydrogel was synthesized from the biopolymers hyaluronic acid (HA), chondroitin sulfate (CS) and gelatin via click chemistry. HA and CS were modified with 11-azido-3,6,9-trioxaundecan-1-amine (AA) and gelatin was modified with propiolic acid (PA). The molecular structures were verified by (1)H nuclear magnetic resonance, infrared spectroscopy and elemental analysis, giving substitution degrees of 29%, 89% and 44% for HA-AA, CS-AA and gelatin-PA (G-PA), respectively. Read More

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TGF-β3 immobilized PLGA-gelatin/chondroitin sulfate/hyaluronic acid hybrid scaffold for cartilage regeneration.

J Biomed Mater Res A 2010 Dec 24;95(4):982-92. Epub 2010 Sep 24.

Department of Orthopaedic Surgery, Xi-jing Hospital, the Fourth Military Medical University, Xi'an, China.

Although most in vitro studies indicate that transforming growth factor β3 (TGF-β3) immobilized scaffold is suitable for cartilage tissue engineering, in vivo studies of implanting immobilized scaffold for chondral defect repair are still lacking. This study is to evaluate the potentials of TGF-β3 immobilized poly-(lactic-co-glycolic acid)-gelatin/chondroitin sulfate/hyaluronic acid (PLGA-GCH) hybrid scaffold for cartilage regeneration. The scaffold was fabricated by incorporating GCH micro-sponges into PLGA frameworks and then crosslinked with TGF-β3 to mimic natural cartilaginous extra cellular matrix (ECM). Read More

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December 2010

Neovascularization by bFGF releasing hyaluronic acid-gelatin microspheres: in vitro and in vivo studies.

Growth Factors 2010 Dec 21;28(6):426-36. Epub 2010 Sep 21.

Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Faculty of Science, Stem Cell Institute, Biotechnology Institute, Ankara University, Ankara, 06100, Turkey.

Therapeutic angiogenesis with angiogenic growth factors has been described as a promising approach for tissue engineering, wound healing, and for treating ischemic tissues. Here, we assessed the merit of heparin-entrapped hyaluronic acid-gelatin (HA-G) microspheres for the sustained release of recombinant basic fibroblast growth factor (rbFGF) to promote localized neovascularization. HA-G microspheres were prepared by a water-in-oil emulsion method, and the in vitro release kinetics were first examined using three model proteins. Read More

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December 2010