466,595 results match your criteria human lymphocytes

The relationship between brachial-ankle pulse wave velocity and peripheral blood lymphocyte subsets in hypertensives: a cross-sectional study.

J Hum Hypertens 2021 Jun 11. Epub 2021 Jun 11.

Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Arteriosclerosis is the common pathological basis of hypertension-related target organ damage, and pulse wave velocity (PWV) is commonly used to assess the degree of arterial stiffness. Previous studies have reported the correlation between peripheral blood inflammatory indicators and PWV in hypertensives, but few studies examined the role of immune cells in arteriosclerosis in the context of human hypertension. In order to enrich the understanding of this topic, we conducted a cross-sectional study on hospitalized hypertensives in Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology from January 2015 to February 2017 to investigate the relationship between brachial-ankle pulse wave velocity (baPWV) and peripheral blood lymphocyte subsets. Read More

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Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches.

Sci Rep 2021 Jun 11;11(1):12397. Epub 2021 Jun 11.

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Cervical cancer, caused by human papillomavirus (HPV), is the fourth most common type of cancer among women worldwide. While HPV prophylactic vaccines are available, they have no therapeutic effects and do not clear up existing infections. This study aims to design a therapeutic vaccine against cervical cancer using reverse vaccinology. Read More

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Mucosal Invariant T cells are Diminished in Very Early-Onset Inflammatory Bowel Disease.

J Pediatr Gastroenterol Nutr 2021 Jun 7. Epub 2021 Jun 7.

Division of Allergy Immunology, Children's Hospital of Philadelphia, Philadelphia, PA 19104 USA Division of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia, Philadelphia, PA 19104 USA.

Objectives: Very early-onset inflammatory bowel disease arises in children less than six years old, a critical time for immunologic development and maturation of the intestinal microbiome. Non-conventional lymphocytes, defined here as mucosal-associated invariant T cells and innate lymphocytes, require microbial products for either development or expansion, aspects that could be altered in very early-onset inflammatory bowel disease. Our objective was to define conventional leukocyte and non-conventional lymphocyte populations in controls and patients using multiparameter flow cytometry to test the hypothesis that their frequencies would be altered in a chronic inflammatory state associated with significant dysbiosis. Read More

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Gut Bacteria Induce Granzyme B Expression in Human Colonic ILC3s In Vitro in an IL-15-Dependent Manner.

J Immunol 2021 Jun 11. Epub 2021 Jun 11.

Division of Infectious Disease, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO;

Group 3 innate lymphoid cells (ILC3s) in the gut mucosa have long been thought to be noncytotoxic lymphocytes that are critical for homeostasis of intestinal epithelial cells through secretion of IL-22. Recent work using human tonsillar cells demonstrated that ILC3s exposed to exogenous inflammatory cytokines for a long period of time acquired expression of granzyme B, suggesting that under pathological conditions ILC3s may become cytotoxic. We hypothesized that inflammation associated with bacterial exposure might trigger granzyme B expression in gut ILC3s. Read More

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Neonatal Lymphopenia Screening Is Important For Early Diagnosis of Severe Combined Immunodeficiency.

Am J Perinatol 2021 Jun 11. Epub 2021 Jun 11.

Division of Hematology and Oncology, Immunology, Department of Pediatrics, Erciyes University, School of Medicine, Kayseri, Turkey.

Objective:  T-cell receptor excision circles are expensive for neonatal severe combined immunodeficiency screening in developing countries. We aimed to detect immunodeficiencies presenting with lymphopenia to enable screening in the general population and to improve awareness regarding lymphopenia among clinicians.

Study Design:  This study was conducted prospectively. Read More

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Intrinsic 5-lipoxygenase activity regulates migration and adherence of mantle cell lymphoma cells.

Prostaglandins Other Lipid Mediat 2021 Jun 8:106575. Epub 2021 Jun 8.

Department of Medicine Solna, Division of Hematology, Karolinska University Hospital and Institutet, Stockholm, Sweden. Electronic address:

Human B-lymphocytes express 5-lipoxygenase (5-LOX) and 5-LOX activating protein (FLAP) and can convert arachidonic acid to leukotriene B. Mantle cell lymphoma (MCL) cells contain similar amounts of 5-LOX as human neutrophils but the function and mechanism of activation of 5-LOX in MCL cells, and in normal B-lymphocytes, are unclear. Here we show that the intrinsic 5-LOX pathway in the MCL cell line JeKo-1 has an essential role in migration and adherence of the cells, which are important pathophysiological characteristics of B-cell lymphoma. Read More

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β-Galactosidase mediated synthesized nanosupport for the immobilization of same enzyme: Its stability and application in the hydrolysis of lactose.

Int J Biol Macromol 2021 Jun 8. Epub 2021 Jun 8.

Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U. P., India. Electronic address:

β-Galactosidase was immobilized on modified nanosilver reduced graphene oxide ([email protected]) nanocomposite prepared by in vitro synthesis using same enzyme. The effectiveness factor, η value of the immobilized enzyme was calculated to be 0.968, suggesting enhancement in enzyme activity after immobilization. Read More

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Diverse functions and mechanisms of regulatory T cell in ischemic stroke.

Exp Neurol 2021 Jun 8:113782. Epub 2021 Jun 8.

Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Peking University, Beijing 100191, China. Electronic address:

The inflammatory and immune processes are key pathophysiological processes in the ischemic stroke, including leukocyte infiltration and destruction of the blood-brain-barrier (BBB), which further lead to increased post-ischemic inflammation. Regulatory T cells (Tregs) are a specific subset of T lymphocytes that play a pivotal role in suppressing the activation of immune system, maintaining immune homeostasis, and regulating inflammation induced by pathogens and environmental toxins. We would like to discuss the paradox function of Tregs in ischemic stroke. Read More

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Induced Pluripotent Stem Cells (iPSCs) Provide a Potentially Unlimited T Cell Source for CAR-T Cell Development and Off-the-Shelf Products.

Pharm Res 2021 Jun 10. Epub 2021 Jun 10.

Nanotechnology & Regenerative Medicine Commercialization Centre (NanoRegMed Ltd), London BioScience Innovation Centre, London, United Kingdom.

Chimeric antigen receptor T (CAR-T) cell therapy has been increasingly conducted for cancer patients in clinical settings. Progress in this therapeutic approach is hampered by the lack of a solid manufacturing process, T lymphocytes, and tumor-specific antigens. T cell source used in CAR-T cell therapy is derived predominantly from the patient's own T lymphocytes, which makes this approach impracticable to patients with progressive diseases and T leukemia. Read More

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Bronchoalveolar Lavage Fluid Cytology in Healthy Cynomolgus Macaques.

Front Vet Sci 2021 25;8:679248. Epub 2021 May 25.

Laboratoire de Biologie Clinique, VetAgro-Sup, Campus vétérinaire Marcy l'Etoile, Marcy L'Etoile, France.

Bronchoalveolar lavage, or BAL, is a minimally invasive procedure frequently used for clinical and non-clinical research, allowing studies of the respiratory system. Macaques are the most widely used non-human primate models in biomedical research. However, very little information is available in the literature concerning BAL cytology in macaques. Read More

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Δ133p53α enhances metabolic and cellular fitness of TCR-engineered T cells and promotes superior antitumor immunity.

J Immunother Cancer 2021 Jun;9(6)

Department of Hematology, Oncology and Pneumology, University Medical Centre of the Johannes Gutenberg University Mainz, Mainz, Germany

Background: Tumor microenvironment-associated T cell senescence is a key limiting factor for durable effective cancer immunotherapy. A few studies have demonstrated the critical role of the tumor suppressor TP53-derived p53 isoforms in cellular senescence process of non-immune cells. However, their role in lymphocytes, in particular tumor-antigen (TA) specific T cells remain largely unexplored. Read More

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iNKT cells coordinate immune pathways to enable engraftment in nonconditioned hosts.

Life Sci Alliance 2021 Jul 10;4(7). Epub 2021 Jun 10.

Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA

Invariant natural killer T (iNKT) cells are a conserved population of innate T lymphocytes that interact with key antigen-presenting cells to modulate adaptive T-cell responses in ways that can either promote protective immunity, or limit pathological immune activation. Understanding the immunological networks engaged by iNKT cells to mediate these opposing functions is a key pre-requisite to effectively using iNKT cells for therapeutic applications. Using a human umbilical cord blood xenotransplantation model, we show here that co-transplanted allogeneic CD4 iNKT cells interact with monocytes and T cells in the graft to coordinate pro-hematopoietic and immunoregulatory pathways. Read More

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[Clinical observation of extremely elevated erythrocyte sedimentation rate].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):613-617

Department of Intensive Care Unit, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China. Corresponding author: Xiao-Li Leyun, Email:

Objective: To analyze the clinical features of adult patients with extremely elevated erythrocyte sedimentation rate (ESR, ESR ≥ 100 mm/1 h), so as improve the ability of clinicians to use erythrocyte sedimentation rate to assist in the diagnosis and treatment of diseases.

Methods: A retrospective cohort study was conducted to examine the clinical data of patients with ESR ≥ 100 mm/1 h admitted to the First Affiliated Hospital of Kunming Medical University from January 1st 2019 to December 31st 2019. The age, gender, clinical diagnosis, first ESR level after admission, blood routine, liver function, renal function, coagulation function and C-reactive protein (CRP) within 24 hours after admission were collected. Read More

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Management of Early-Stage Human Epidermal Growth Factor Receptor 2-Positive Breast Cancer.

JCO Oncol Pract 2021 Jun;17(6):320-330

Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA.

The addition of trastuzumab to chemotherapy dramatically improved the prognosis of early-stage human epidermal growth factor receptor 2 (HER2)-positive breast cancer. However, 15%-31% of patients still develop disease recurrence, on the basis of long-term follow-up of adjuvant pivotal trials. A better understanding of tumor biology has led to the development of optimized anti-HER2 drugs and add-on strategies to further improve survival outcomes. Read More

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Spontaneous (Hashimoto-like) chronic lymphocytic thyroiditis in a rhesus macaque ().

Primate Biol 2021 1;8(1):37-42. Epub 2021 Jun 1.

Zentrallabor, Universitätsklinikum Frankfurt, Frankfurt, Germany.

A case of a female, 10-year-old rhesus macaque () with spontaneous chronic lymphocytic thyroiditis is presented. At necropsy, the thyroid gland was slightly enlarged, with up to 2 mm large, round, confluent, beige foci on the surface of both lobes. Histopathologic features resembled human Hashimoto's thyroiditis: multifocally, the interstitium was infiltrated by lymphocytes and variably sized lymphoid follicles. Read More

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Cesk Slov Oftalmol 2020 ;1(Ahead of print):1-6

Immune mediated inflammatory diseases are categorized into autoimmune and autoinflammatory. Autoimmune etiology is represented by autoreactive lymphocytes or autoantibodies, e.g. Read More

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[Effect of tenofovir disoproxil fumarate antiviral therapy on virus-specific CD8+T Cells function in patients with chronic hepatitis B].

Zhonghua Gan Zang Bing Za Zhi 2021 May;29(5):421-426

Medical Section, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China.

To observe the effect of tenofovir disoproxil fumarate (TDF) antiviral therapy on HBV-specific CD8(+)T cell function in peripheral blood of patients with HBeAg-positive chronic hepatitis B, and to assess its correlation with HBeAg sero-negativeness. Sixty-three cases with HLA-A02 restricted HBeAg-positive chronic hepatitis B who received TDF (300 mg/d) antiviral therapy were enrolled from October 2016 to July 2018. The peripheral blood CD8(+)T cells were separated at baseline and 48 weeks after treatment. Read More

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Specific features of T- and NK-cellular immunity in chronic lymphocytic leukemia.

Klin Lab Diagn 2021 Jun;66(6):345-352

Russian Medical Academy of professional continuous education.

Profound immunological dysfunction is the key factor determining the development of infectious complications in chronic lymphocytic leukemia (CLL). The aim of this work is to assess the features of the subpopulation composition of T-lymphocytes (T-helpers (Th), cytotoxic T-lymphocytes (Tcyt), T regulatory cells (Treg), T-NK cells, naive Th, Th-memory, activated T-lymphocytes, TCRγδ cells) and NK cells in peripheral blood of patients with newly diagnosed chronic lymphocytic leukemia (CLL) and receiving ibrutinib therapy. Hematological and immunophenotypic studies have been performed in 30 patients with previously untreated CLL, 122 patients on ibrutinib therapy and 20 healthy donors. Read More

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[Establishment and Usage of Leukemia-Lymphoma Cell Lines--Review].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):1007-1010

Department of Hematology, Affiliated Hospital of Jiangsu University, Zhenjiang 212000, Jiangsu Province, China,E-mail:

Cancer cell lines are an indispensable tool in the cancer research. Since the first human cell line, HeLa was established in the 1950s, thousands of cancer cell lines have been established, including 637 characterized leukemia-lymphoma cell lines. The probability to successfully establish cancer cell lines is a low by traditional methods, and the addition of regulatory factors is often required. Read More

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[Research Advance of Immune Checkpoint Molecule TIGIT in Hematological Malignancies--Review].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):993-997

Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430020, Hubei Province, China,E-mail:

TIGIT is an inhibitory receptor containing T cell immunoglobulin and immune receptor protein tyrosine inhibitory motif domain. It shows high expression level on the surface of immune cells in tumor patients and plays an inhibitory role by binding to corresponding ligands, CD155 and CD112. Studying the mechanism of inhibitory effect of TIGIT and the way to block it shows a great significance in the immunotherapy of tumor. Read More

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[Exhaustion of CD8T Lymphocytes Plays a Critical Role in the Pathogenesis of Secondary Hemophagocytic Lymphohistiocytosis].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):963-968

Department of Hematology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China,E-mail:

Objective: To investigate the changes in function of CD8 T cell subsets, and explore its significance in pathogenesis of secondary hemophagocytic lymphohistiocytosis (sHLH).

Methods: Flow cytometry was used to detect the expressions of PD-1, TIM-3, and LAG-3, which were the markers of exhausted CD8 T cell, as well as the secretion levels of interferon γ (IFN-γ) and expression of ΔCD107a after the stimulation; the numbers of effector-memory CD8 T cells, regulatory T cells and double negative T cells were also detected.

Results: The expressions of inhibitory receptors (PD-1, TIM3 and LAG-3) on CD8 T cells of sHLH patients were 40. Read More

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[Clinical Analysis of CMV Infection after Allogeneic Hematopoietic Stem Cell Transplantation in Severe Aplastic Anemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):944-950

Department of Hematology, Guangzhou NO.1 People's Hospital, Guangzhou Medical University,Guangzhou 510180, Guangdong Province, China,E-mail:

Objective: To investigate the clinical characteristics and risk factors of cytomegalovirus (CMV) infection after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients with severe aplastic anemia (SAA).

Methods: Clinical data from 270 SAA patients with allo-HSCT were retrospectively analyzed, including 108 sib congruence patients and 162 substitute donors (68 unrelated donor congruence patients and 94 related haploid patients). Different pretreatment schemes were selected for different transplantation modes. Read More

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[Influencing Factors in the Chronicity of Immune Thrombocytopenia in Children].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):881-886

Department of Pediatric Hematology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, Anhui Province, China,E-mail: zwnltt

Objective: To explore the influencing factors in children with chronicity immune thrombocytopenia (ITP), and to provide basis for judging the prognosis and treatment in children with ITP.

Methods: The clinical data of children with ITP admitted to The Second Affiliated Hospital of Anhui Medical University in the past 5 years were retrospectively analyzed and followed up for more than 1 year. According to the inclusion criteria, the eligible cases (328 cases in total) were selected and collected through medical record system retrieval, outpatient clinic and telephone follow-up. Read More

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[The Influence of CONUT Score on the Prognosis of Patients with Multiple Myeloma].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):781-786

Department of Hematology, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China,E-mail:

Objective: To explore the influence of controlling nutritional status (CONUT) score on the prognosis of newly diagnosed patients with multiple myeloma (MM).

Methods: The clinical data 119 patients with MM who were diagnosed according to the international myeloma diagnostic criteria in Lanzhou University Second Hospital from April 2010 to October 2018 were collected and retrospectively analyzed. The relationship between clinical indexes, including age, sex, MM type, absolute lymphocyte count (ALC), absolute neutrophil count (ANC), absolute monocyte count (AMC), hemoglobin (Hb), platelet (PLT), β-microglobulin (β-MG), lactate dehydrogenase (LDH), albumin (ALB), globulin (GLO), cholesterol (CHO), serum creatinine (Scr), etc, and CONUT score was discussed to explore the prognostic value of these indicators. Read More

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[The Genome-Wide Changes in Expression Profile of CML T Cells After Up-regulation of TCRζ Chain Expression].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):669-676

Department of Clinical Laboratory, School of Medicine, First Affiliated Hospital, Jinan University; Guangzhou 510632, Guangdong Province, China,E-mail:

Objective: To analyze the changes in the gene expression profile of T cells in CML patients after TCRζ up-regulation expression, and to explore the molecular mechanism of T cell reactivation after transgenic up-regulation of TCRζ.

Methods: The peripheral blood mononuclear cells(PBMCs) from 3 newly untreated chronic-stage CML patients were collected, and the CD3 T cells were obtained by MACS method. The TCRζ-IRES2-EGFP (experimental group) and pIRES2-EGFP (control group) plasmids were transfected into T cells by nuclear transfection technique. Read More

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Generation of neoantigen-specific T cells for adoptive cell transfer for treating head and neck squamous cell carcinoma.

Oncoimmunology 2021 May 25;10(1):1929726. Epub 2021 May 25.

Cytotherapy Laboratory, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; the First Affiliated Hospital, Southern University of Science and Technology), Shenzhen Guangdong, China.

Adoptive cell therapy using TCR-engineered T cells (TCR-T cells) represents a promising strategy for treating relapsed and metastatic cancers. We previously established methods to identify neoantigen-specific TCRs based on patients' PBMCs. However, in clinical practice isolation of PBMCs from advanced-stage cancer patients proves to be difficult. Read More

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Human Leukocyte Antigen-DR Isotype Expression in Monocytes and T Cells Interferon-Gamma Release Assay in Septic Patients and Correlation With Clinical Outcome.

J Clin Med Res 2021 May 25;13(5):293-303. Epub 2021 May 25.

Clinical Pathology and Microbiology, Vito Fazzi General Hospital ASL-Lecce, Lecce, Italy.

Background: Sepsis is a life-threatening dysregulated host response to infection responsible of multiple organs dysfunction (Sepsis-3 International Consensus Definition), during which clinical outcome is a balance between inflammation and immune suppression. Monocytes and lymphocytes may play an important role in immune paralysis, and their impaired functional activity can decrease overall immune system efficiency. We evaluated sepsis-induced changes in monocytes human leukocyte antigen-DR isotype (HLA-DR) expression and T cell capacity of interferon (IFN)-γ production in relation with patient's clinical outcome. Read More

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Analysis of the transcriptional activity of genes of neuropeptides and their receptors in the blood of patients with thyroid pathology.

J Med Life 2021 Mar-Apr;14(2):243-249

Department of Microbiology, Virology, and Immunology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine.

The thyroid hormone plays a vital role in the development and maturation of the nervous system not only during prenatal and perinatal age but also in adults. "Peripheral marker hypothesis" revealed that gene expression changes in some regions of the brain are reflected into the peripheral blood lymphocytes. The objective of the study was to investigate changes in the gene expression profile of neuropeptides and their receptors in patients with different forms of thyroid pathology. Read More

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Vitiligo and chronic autoimmune thyroiditis.

J Med Life 2021 Mar-Apr;14(2):127-130

Department of Endocrinology, Clinical County Hospital, Cluj-Napoca, Romania.

Vitiligo, the discoloration of the skin, has different autoimmune mechanisms reflected by many biomarkers as shown by skin histology, staining for CD4 and CD8 T lymphocytes, chemokine ligand 9 or circulating cytokines such as interleukin (IL)-1 beta, interferon (IFN)-gamma, transforming growth factor (TGF)-beta, antibodies, markers of oxidative stress, chemokines, and others. In this narrative review, we aim to overview vitiligo in relationship with chronic autoimmune thyroiditis. Regarding vitiligo, more than 50 different genetic loci have been associated with this disease, and the heritability is high. Read More

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-Induced IFN-Gamma Responses in Peripheral Blood Mononuclear Cells Increase Numbers of CD4+ but Not CD8+ T Effector Memory Cells.

J Blood Med 2021 31;12:385-394. Epub 2021 May 31.

Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Medical Center, Brooklyn, NY, 11203, USA.

Background: causes respiratory infection in adults and children. Previous studies in our laboratory identified significantly higher in vitro T lymphocyte responses to in children with asthma compared to healthy controls which may indicate the presence of T effector memory (TEM) lymphocytes.

Aim: In the present study, healthy subjects were screened for the presence of TEM cells and their cytokines. Read More

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