1,322,154 results match your criteria human growth


RNF168 promotes RHOC degradation by ubiquitination to restrain gastric cancer progression via decreasing HDAC1 expression.

Biochem Biophys Res Commun 2021 Apr 14;557:135-142. Epub 2021 Apr 14.

Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an Jiangsu, 223300, China. Electronic address:

Gastric cancer (GC) is the most common cancer worldwide. Although advances in the treatments, the oncogenic mechanisms are still largely unknown. RNF168 (ring-finger nuclear factor 168) is an important regulator of DNA double-strand break (DSB) repair, and its defects have been involved in the pathogenesis of a number of human diseases including cancer. Read More

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Stimulation of sewage sludge treatment by carbon sources and bioaugmentation with a sludge-derived microbial consortium.

Sci Total Environ 2021 Apr 9;783:146989. Epub 2021 Apr 9.

Institute of Microbiology & Biotechnology, University of Latvia, 1 Jelgavas Str., Riga LV-1004, Latvia. Electronic address:

Recently, sewage sludge (SS) disposal has become one of the greatest global challenges. In this study, we aimed to evaluate the effect of faba bean straw (Straw-B), wheat straw (Straw-W), and wood-chip pellets (WCP) amended to SS, as well as bioaugmentation (BA), on the physicochemical characteristics and structure of the microbial community of the treated SS. Sixteen days of incubation of SS-containing mixtures revealed the highest efficiency of Straw-W(BA) in terms of SS stabilisation, i. Read More

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Editorial: Symbiogenesis and progressive evolution.

Biosystems 2021 Apr 14:104429. Epub 2021 Apr 14.

Department of Biology, Memorial University of Newfoundland, St. John's, NL, A1B 3X9, Canada. Electronic address:

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LEP (-2548G>A LEP) and LEPR (223Gln>Arg, 109Lys>Arg) polymorphisms as breast cancer risk factors in the Polish female population.

Mol Biol Rep 2021 Apr 17. Epub 2021 Apr 17.

Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, 49 Przybyszewskiego St., 60-355, Poznan, Poland.

On a global scale, breast cancer is the most common type of cancer in women, and it is still a growing problem. Therefore, new prognostic or diagnostic markers are required that would facilitate the assessment of patients or provide more efficient therapy, respectively. In these studies, we analyzed the contribution of LEP (2548G>A) and LEPR (109 Lys>Arg and 223Gln>Arg) genes polymorphisms to the risk of breast cancer development. Read More

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Carbohydrate analysis of Mortierella alpina by colorimetry and HPLC-ELSD to reveal accumulation differences of sugar and lipid.

Biotechnol Lett 2021 Apr 17. Epub 2021 Apr 17.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.

Objectives: To establish reliable methods for the extraction and quantification of the total carbohydrate and intracellular saccharides from Mortierella alpina and study the changes between carbohydrate and lipid in fermentation process.

Results: The extraction of mycelia with HCl following a photometric phenol-sulphuric acid reaction was identified as an optimal method for total carbohydrate analysis in Mortierella alpina, which the extraction efficiency performed 1.1-3. Read More

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Anti-carcinogenic effects of exercise-conditioned human serum: evidence, relevance and opportunities.

Eur J Appl Physiol 2021 Apr 17. Epub 2021 Apr 17.

School of Biomedical, Nutritional and Sport Sciences, Faculty of Medical Sciences, Newcastle University Centre for Cancer, Newcastle University, Newcastle upon Tyne, UK.

Regular physical activity reduces the risk of several site-specific cancers in humans and suppresses tumour growth in animal models. The mechanisms through which exercise reduces tumour growth remain incompletely understood, but an intriguing and accumulating body of evidence suggests that the incubation of cancer cells with post-exercise serum can have powerful effects on key hallmarks of cancer cell behaviour in vitro. This suggests that exercise can impact tumour biology through direct changes in circulating proteins, RNA molecules and metabolites. Read More

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Furin is involved in uterine activation for labor.

FASEB J 2021 May;35(5):e21565

Department of Gynecology and Obstetrics, Research Center for Molecular Metabolomics, Xiangya Hospital Central South University, Changsha, China.

The uterus undergoes distinct molecular and functional changes during pregnancy and parturition. These processes are associated with the dramatic changes in various proteins. Given that the maturation and activation of many proteins require proteolytic processing by proprotein convertases (PCs), we sought to explore the role of PCs in uterine activation for labor. Read More

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Rare deleterious de novo missense variants in RNF2/RING2 are associated with a neurodevelopmental disorder with unique clinical features.

Hum Mol Genet 2021 Apr 16. Epub 2021 Apr 16.

Department of Molecular and Human Genetics, Baylor College of Medicine (BCM), Houston, TX, USA.

The Polycomb group (PcG) gene RNF2 (RING2) encodes a catalytic subunit of the Polycomb Repressive Complex 1 (PRC1), an evolutionarily conserved machinery that post-translationally modifies chromatin to maintain epigenetic transcriptional repressive states of target genes including Hox genes. Here, we describe two individuals, each with rare de novo missense variants in RNF2. Their phenotypes include intrauterine growth retardation, severe intellectual disabilities, behavioral problems, seizures, feeding difficulties and dysmorphic features. Read More

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Health screening system to ensure the children' health and development in Japan.

Pediatr Int 2021 Apr 16. Epub 2021 Apr 16.

Japanese Consortium for Global School Health and Research, 207 Uehara, Nishihara, Nakazu-gun, Okinawa, 903-0215, Japan.

Introduction: Establishment of health screening system for children is important. However, the systems are not always well-established in developing countries. Japan has conducted health screenings for children at the nationwide level. Read More

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Population Pharmacokinetics and Pharmacodynamics of Once-Daily Growth Hormone Norditropin in Children and Adults.

Clin Pharmacokinet 2021 Apr 17. Epub 2021 Apr 17.

Global Development, Novo Nordisk A/S, 2860, Søborg, Denmark.

Background And Objective: Once-daily injectable recombinant human growth hormone (GH) formulations (e.g. Norditropin; Novo Nordisk A/S) are used to treat GH deficiency in children and adults, with much of the therapeutic effect mediated via the insulin-like growth factor-I (IGF-I) response. Read More

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LRRC8A influences the growth of gastric cancer cells via the p53 signaling pathway.

Gastric Cancer 2021 Apr 16. Epub 2021 Apr 16.

Department of Surgery, Division of Digestive Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto, 602-8566, Japan.

Background: Leucin-rich repeat containing protein A (LRRC8A), a component of the volume-regulated anion channel (VRAC), is activated by cell swelling and mediates regulatory volume decrease. We previously reported the expression of and important roles for several ion transporters in various gastrointestinal cancers, which have potential as novel targets for cancer treatment; however, the significance of LRRC8A in gastric cancer (GC) remains unclear.

Materials And Methods: Knockdown experiments were performed by transfecting human GC cell lines with LRRC8A siRNA. Read More

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Markers of vitamin D metabolism and premenstrual symptoms in healthy women with regular cycles.

Hum Reprod 2021 Apr 16. Epub 2021 Apr 16.

Epidemiology Branch, Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.

Study Question: Are insufficient 25-hydroxyvitamin D (25(OH)D) concentrations, and other markers of vitamin D metabolism, associated with premenstrual symptoms in healthy women with regular menstrual cycles?

Summary Answer: 25(OH)D insufficiency was associated with specific physical premenstrual symptoms, while no associations were observed with psychological symptoms or with other markers of vitamin D metabolism.

What Is Known Already: Prior studies evaluating vitamin D and premenstrual symptoms have yielded mixed results, and it is unknown whether 25(OH)D insufficiency and other markers of vitamin D metabolism are associated with premenstrual symptoms.

Study Design, Size, Duration: We used two cohorts of women with regular menstrual cycles; 1191 women aged 18-40 years in EAGeR (cross-sectional analysis of a prospective cohort within a randomized trial) and 76 women aged 18-44 years in BioCycle (prospective cohort). Read More

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Directed differentiation of human induced pluripotent stem cells to hepatic stellate cells.

Nat Protoc 2021 Apr 16. Epub 2021 Apr 16.

Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.

Hepatic stellate cells (HSCs) are nonparenchymal liver cells responsible for extracellular matrix homeostasis and are the main cells involved in the development of liver fibrosis following injury. The lack of reliable sources of HSCs has hence limited the development of complex in vitro systems to model liver diseases and toxicity. Here we describe a protocol to differentiate human induced pluripotent stem cells (iPSCs) into hepatic stellate cells (iPSC-HSCs). Read More

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Computational modelling of perivascular-niche dynamics for the optimization of treatment schedules for glioblastoma.

Nat Biomed Eng 2021 Apr 16;5(4):346-359. Epub 2021 Apr 16.

Department of Data Science, Dana-Farber Cancer Institute, Boston, MA, USA.

Glioblastoma stem-like cells dynamically transition between a chemoradiation-resistant state and a chemoradiation-sensitive state. However, physical barriers in the tumour microenvironment restrict the delivery of chemotherapy to tumour compartments that are distant from blood vessels. Here, we show that a massively parallel computational model of the spatiotemporal dynamics of the perivascular niche that incorporates glioblastoma stem-like cells and differentiated tumour cells as well as relevant tissue-level phenomena can be used to optimize the administration schedules of concurrent radiation and temozolomide-the standard-of-care treatment for glioblastoma. Read More

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On the origin and evolution of SARS-CoV-2.

Exp Mol Med 2021 Apr 16. Epub 2021 Apr 16.

School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the ongoing global outbreak of a coronavirus disease (herein referred to as COVID-19). Other viruses in the same phylogenetic group have been responsible for previous regional outbreaks, including SARS and MERS. SARS-CoV-2 has a zoonotic origin, similar to the causative viruses of these previous outbreaks. Read More

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CRISPR/Cas9-mediated knockout of PIM3 suppresses tumorigenesis and cancer cell stemness in human hepatoblastoma cells.

Cancer Gene Ther 2021 Apr 16. Epub 2021 Apr 16.

Division of Pediatric Surgery, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, 35233, USA.

Hepatoblastoma remains one of the most difficult childhood tumors to treat and is alarmingly understudied. We previously demonstrated that Proviral Insertion site in Maloney murine leukemia virus (PIM) kinases, specifically PIM3, are overexpressed in human hepatoblastoma cells and function to promote tumorigenesis. We aimed to use CRISPR/Cas9 gene editing with dual gRNAs to introduce large inactivating deletions in the PIM3 gene and achieve stable PIM3 knockout in the human hepatoblastoma cell line, HuH6. Read More

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Isolation and characterisation of lymphatic endothelial cells from lung tissues affected by lymphangioleiomyomatosis.

Sci Rep 2021 Apr 16;11(1):8406. Epub 2021 Apr 16.

Division of Respiratory Medicine, Juntendo University Faculty of Medicine and Graduate School of Medicine, 3-1-3 Hongo; Bunkyoku, Tokyo, 113-8431, Japan.

Lymphangioleiomyomatosis (LAM) is a rare pulmonary disease characterised by the proliferation of smooth muscle-like cells (LAM cells), and an abundance of lymphatic vessels in LAM lesions. Studies reported that vascular endothelial growth factor-D (VEGF-D) secreted by LAM cells contributes to LAM-associated lymphangiogenesis, however, the precise mechanisms of lymphangiogenesis and characteristics of lymphatic endothelial cells (LECs) in LAM lesions have not yet been elucidated. In this study, human primary-cultured LECs were obtained both from LAM-affected lung tissues (LAM-LECs) and normal lung tissues (control LECs) using fluorescence-activated cell sorting (FACS). Read More

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Combined effects of crude oil exposure and warming on eggs and larvae of an arctic forage fish.

Sci Rep 2021 Apr 16;11(1):8410. Epub 2021 Apr 16.

Department of Arctic and Marine Biology, UiT The Arctic University of Norway, 9037, Tromsø, Norway.

Climate change, along with environmental pollution, can act synergistically on an organism to amplify adverse effects of exposure. The Arctic is undergoing profound climatic change and an increase in human activity, resulting in a heightened risk of accidental oil spills. Embryos and larvae of polar cod (Boreogadus saida), a key Arctic forage fish species, were exposed to low levels of crude oil concurrently with a 2. Read More

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uPAR antibody (huATN-658) and Zometa reduce breast cancer growth and skeletal lesions.

Bone Res 2020 Apr 17;8(1):18. Epub 2020 Apr 17.

Department of Medicine, McGill University, Montréal, QC, H4A3J1, Canada.

Urokinase plasminogen activator receptor (uPAR) is implicated in tumor growth and metastasis due to its ability to activate latent growth factors, proteases, and different oncogenic signaling pathways upon binding to different ligands. Elevated uPAR expression is correlated with the increased aggressiveness of cancer cells, which led to its credentialing as an attractive diagnostic and therapeutic target in advanced solid cancer. Here, we examine the antitumor effects of a humanized anti-uPAR antibody (huATN-658) alone and in combination with the approved bisphosphonate Zometa (Zoledronic acid) on skeletal lesion through a series of studies in vitro and in vivo. Read More

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Cellular senescence in pathologic retinal angiogenesis.

Trends Endocrinol Metab 2021 Apr 13. Epub 2021 Apr 13.

Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

Pathologic angiogenesis causes blindness in many eye diseases. Crespo-Garcia, Tsuruda, and Dejda et al. employed bioinformatics to characterize cell senescence as a primary factor in the common pathogenesis of retinopathies. Read More

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Blocking miR-27a-3p sensitises Taxol resistant osteosarcoma cells through targeting Fbxw7.

Bull Cancer 2021 Apr 13. Epub 2021 Apr 13.

Weifang people's hospital, Department of spinal surgery, 261041 Weifang city, Shandong province, China. Electronic address:

Osteosarcoma (OS) is a human malignancy, which primarily affects the long bones and occurs in children and adolescent. Although advanced clinical approaches and the addition of neoadjuvant chemotherapy improved 5-year survival of OS patients, a large fraction of them developed chemoresistance. Thus, due to the high morbidity and mortality of OS, it is urgent to investigate effectively molecular targets against chemoresistant osteosarcoma. Read More

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A comparative study of leaf nutrient concentrations in a regional herbaceous flora.

New Phytol 1997 Aug;136(4):679-689

NERC Unit of Comparative Plant Ecology, Department of Animal and Plant Sciences, The University, Sheffield S10 2TN, UK.

Mineral nutrient concentrations were determined in leaves of 83 mostly herbaceous species collected from central England. Most samples were analysed for N, P, K, Ca, Mg, Na, Fe, Al, Mn, Cu and Zn. Concentrations of K, N and P showed similar levels of interspecific variability, with the highest concentrations being 6-9 times the lowest. Read More

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The Caenorhabditis elegans homolog of human mitochondrial pyrimidine nucleotide transporter regulates glucose transport.

Biochem Biophys Res Commun 2021 Apr 13;557:117-121. Epub 2021 Apr 13.

Department of Chemistry and Bioengineering, Iwate University, 4 Ueda, Morioka, Iwate, 020-8551, Japan.

Caenorhabditis elegans T09F3.2 is a homolog of the human mitochondrial pyrimidine nucleotide transporter. We isolated a T09F3. Read More

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Hyperspectral monitor of soil chromium contaminant based on deep learning network model in the Eastern Junggar coalfield.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 26;257:119739. Epub 2021 Mar 26.

College of Atmospheric Sciences, Lanzhou University, Lanzhou 730000, China.

In China, over 10% of cultivated land is polluted by heavy metals, which can affect crop growth, food safety and human health. Therefore, how to effectively and quickly detect soil heavy metal pollution has become a critical issue. This study provides a novel data preprocessing method that can extract vital information from soil hyperspectra and uses different classification algorithms to detect levels of heavy metal contamination in soil. Read More

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Structural and functional characterization of NDBP-4 family antimicrobial peptides from the scorpion Mesomexovis variegatus.

Peptides 2021 Apr 13:170553. Epub 2021 Apr 13.

Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad, 2001, Colonia Chamilpa, Cuernavaca, Morelos, 62210, Mexico. Electronic address:

Six peptides, belonging to the NDBP-4 family of scorpion antimicrobial peptides were structurally and functionally characterized. The sequence of the mature peptides VpCT1, VpCT2, VpCT3 and VpCT4 was inferred by transcriptomic analysis of the venom gland of the scorpion Mesomexovis variegatus. Analysis of their amino acid sequences revealed patterns that are also present in previously reported peptides that show differences in their hemolytic and antimicrobial activities in vitro. Read More

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ESDN inhibits melanoma progression by blocking E-selectin expression in endothelial cells via STAT3.

Cancer Lett 2021 Apr 13. Epub 2021 Apr 13.

Molecular Biotechnology Center (MBC), University of Leuven, Leuven, Belgium; Dept. Molecular Biotechnology and Health Sciences, University of Leuven, Leuven, Belgium. Electronic address:

An interactive crosstalk between tumor and stroma cells is essential for metastatic melanoma progression. We evidenced that ESDN/DCBLD2/CLCP1 plays a crucial role in endothelial cells during the spread of melanoma. Precisely, increased extravasation and metastasis formation was revealed in ESDN-null mice injected with melanoma cells, even if the primary tumor growth, vessel permeability, and angiogenesis were not enhanced. Read More

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Corilagin alleviates hypertrophic scars via inhibiting the transforming growth factor (TGF)-β/Smad signal pathway.

Life Sci 2021 Apr 13:119483. Epub 2021 Apr 13.

Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Aims: Exploring the effects of corilagin on hypertrophic scar (HS) and its underlying mechanisms.

Main Methods: Human HS-derived fibroblasts (HSFs) were isolated and treated with corilagin. To investigate the effects of corilagin on HSFs, quantitative real time polymerase chain reaction (qRT-PCR), western blotting, wound healing, and immunofluorescence assays were performed. Read More

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Piperlongumine inhibits the progression of osteosarcoma by downregulating the SOCS3/JAK2/STAT3 pathway via miR-30d-5p.

Life Sci 2021 Apr 13:119501. Epub 2021 Apr 13.

Department of Spine Surgery, People's Hospital of Longhua, Affiliated Hospital of Southern Medical University, Guangdong 518000, Shenzhen, China. Electronic address:

Aims: The present study evaluated the functions of Piperlongumine (PL) in osteosarcoma (OS) cell growth and metastasis both in vitro and in vivo.

Main Methods: MTT assay was conducted to test the cytotoxic effects of PL on the human osteoblasts line HFOB1.19 and the human normal chondrocyte line C28/I2T. Read More

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Validation of a host blood transcriptomic biomarker for pulmonary tuberculosis in people living with HIV: a prospective diagnostic and prognostic accuracy study.

Lancet Glob Health 2021 Apr 13. Epub 2021 Apr 13.

South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town, Cape Town, South Africa. Electronic address:

Background: A rapid, blood-based triage test that allows targeted investigation for tuberculosis at the point of care could shorten the time to tuberculosis treatment and reduce mortality. We aimed to test the performance of a host blood transcriptomic signature (RISK11) in diagnosing tuberculosis and predicting progression to active pulmonary disease (prognosis) in people with HIV in a community setting.

Methods: In this prospective diagnostic and prognostic accuracy study, adults (aged 18-59 years) with HIV were recruited from five communities in South Africa. Read More

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Endothelial Cells Differentiated from Porcine Epiblast Stem Cells.

Cell Reprogram 2021 Apr;23(2):89-98

Division of Life Science, College of Natural Sciences, Gyeongsang National University, Jinju, Republic of Korea.

Pluripotent stem cells (PSCs) have the ability of self-renewal that can retain the characteristics of the mother cell, and of pluripotency that can differentiate into several body types. PSCs typically include embryonic stem cells (ESCs) derived from the inner cell mass of the preimplantation embryo, and epiblast stem cells (EpiSCs) derived from the epiblast of postimplantation embryo. Although PSCs are able to be used by differentiation into endothelial cells as a potential treatment for vascular diseases, human ESCs and induced PSCs (iPSCs) are followed by ethical and safety issues. Read More

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