20,015,101 results match your criteria human embryonic

The influence of castration on intramural neurons of the urinary bladder trigone in male pigs.

J Anat 2021 May 10. Epub 2021 May 10.

Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

The present study investigated the influence of castration performed at neonatal age on neuronal elements in the intramural ganglia of the urinary bladder trigone (UBT) in male pigs using double-labeling immunohistochemistry. The ganglia were examined in intact (IP) 7-day-old (castration day) pigs, and at 3 and 6 months after surgery. In IP and control (3- and 6-month-old noncastrated pigs) groups, virtually, all neurons were adrenergic (68%) or cholinergic (32%) in nature. Read More

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Pan-cancer application of a lung-adenocarcinoma-derived gene-expression-based prognostic predictor.

Brief Bioinform 2021 May 10. Epub 2021 May 10.

Lund University, Sweden.

Gene-expression profiling can be used to classify human tumors into molecular subtypes or risk groups, representing potential future clinical tools for treatment prediction and prognostication. However, it is less well-known how prognostic gene signatures derived in one malignancy perform in a pan-cancer context. In this study, a gene-rule-based single sample predictor (SSP) called classifier for lung adenocarcinoma molecular subtypes (CLAMS) associated with proliferation was tested in almost 15 000 samples from 32 cancer types to classify samples into better or worse prognosis. Read More

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Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses.

Nature 2021 May 10. Epub 2021 May 10.

Division of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration, Silver Spring, MD, USA.

Betacoronaviruses (betaCoVs) caused the severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS) outbreaks, and the SARS-CoV-2 pandemic. Vaccines that elicit protective immunity against SARS-CoV-2 and betaCoVs circulating in animals have the potential to prevent future betaCoV pandemics. Here, we show that macaque immunization with a multimeric SARS-CoV-2 receptor binding domain (RBD) nanoparticle adjuvanted with 3M-052/Alum elicited cross-neutralizing antibody (cross-nAb) responses against batCoVs, SARS-CoV-1, SARS-CoV-2, and SARS-CoV-2 variants B. Read More

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Risk of Vaccine-Preventable Infections in Swiss Adults with Inflammatory Bowel Disease.

Digestion 2021 May 10:1-9. Epub 2021 May 10.

Department of Pediatrics, Pediatric Infectious Disease Unit, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.

Background: Patients with inflammatory bowel disease (IBD) have a higher risk of infection and are frequently not up to date with their immunizations.

Objectives: This study aims to review vaccination status and evaluate whether age, disease type, or treatment regimen could predict the absence of seroprotection against selected vaccine-preventable infection in adults with IBD.

Methods: Cross-sectional study using questionnaire, immunization records review, and assessment of tetanus-specific, varicella-specific, and measles-specific immunoglobulin G concentrations. Read More

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Emerging machine learning approaches to phenotyping cellular motility and morphodynamics.

Phys Biol 2021 May 10. Epub 2021 May 10.

Vascular Biology Program, Boston Children's Hospital, 300 Longwood Ave, Boston, Massachusetts, 02115-5724, UNITED STATES.

Cells respond heterogeneously to molecular and environmental perturbations. Phenotypic heterogeneity, wherein multiple phenotypes coexist in the same conditions, presents challenges when interpreting the observed heterogeneity. Advances in live cell microscopy allow researchers to acquire an unprecedented amount of live cell image data at high spatiotemporal resolutions. Read More

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The expression of mimecan in adrenal tissue plays a role in an organism's responses to stress.

Aging (Albany NY) 2021 May 10;13. Epub 2021 May 10.

The Core Laboratory in Medical Center of Clinical Research, Department of Molecular Diagnostic and Endocrinology, Shanghai Ninth People’s Hospital, State Key Laboratory of Medical Genomics, Shanghai Jiao tong University School of Medicine, Shanghai 200011, China.

Mimecan encodes a secretory protein that is secreted into the human serum as two mature proteins with molecular masses of 25 and 12 kDa. We found 12-kDa mimecan to be a novel satiety hormone mediated by the upregulation of the expression of interleukin (IL)-1β and IL-6 in the hypothalamus. Mimecan was found to be expressed in human pituitary corticotroph cells and was up-regulated by glucocorticoids, while the secretion of adrenocorticotropic hormone (ACTH) in pituitary corticotroph AtT-20 cells was induced by mimecan. Read More

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Experiences of responsiveness to exercise in people with chronic whiplash: A qualitative study.

Musculoskelet Sci Pract 2021 Apr 21;54:102380. Epub 2021 Apr 21.

Faculty of Medicine & Health, The University of Sydney, NSW, Australia; John Walsh Centre for Rehabilitation Research, Royal North Shore Hospital, NSW, Australia. Electronic address:

Exercise is recommended in clinical guidelines for the treatment of chronic whiplash associated disorders (WAD). However, randomised controlled trials have shown similar effects for comprehensive exercise programs and advice. To date, there is no clear understanding of why some individuals with WAD appear to respond to exercise whilst others do not. Read More

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Polycyclic aromatic compounds (PAHs, oxygenated PAHs, nitrated PAHs, and azaarenes) in air from four climate zones of China: Occurrence, gas/particle partitioning, and health risks.

Sci Total Environ 2021 May 1;786:147234. Epub 2021 May 1.

Institute of Geography and Geoecology, Karlsruhe Institute of Technology (KIT), Reinhard-Baumeister-Platz 1, 76131 Karlsruhe, Germany.

Polycyclic aromatic compounds (PACs) such as polycyclic aromatic hydrocarbons (PAHs) and their derivatives [oxygenated PAHs (OPAHs), nitrated PAHs (NPAHs), and azaarenes (AZAs)] are toxic and ubiquitous air pollutants. In this study, the concentrations of these PACs were determined in air obtained in spring and autumn of 2012 from urban and rural areas of the Tibetan Plateau, temperate, subtropical, and tropical climate zones in China. Average concentrations (gaseous + particulate) of ∑29PAHs, ∑15OPAHs, ∑11NPAHs, and ∑4AZAs were 928 ± 658, 54 ± 45, 5. Read More

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Soil type shapes the antibiotic resistome profiles of long-term manured soil.

Sci Total Environ 2021 Apr 27;786:147361. Epub 2021 Apr 27.

College of Resources and Environment, Southwest University, Chongqing 400716, China. Electronic address:

Animal manure fertilization facilitates the proliferation and dissemination of antibiotic resistance genes (ARGs) in soil, posing high risks to humans and ecosystem health. Although studies suggest that soil types could shape the ARG profiles in greenhouse soil, there is still a lack of comparative studies on the fate of ARGs in different types of manured soils under field trials. Thus, a metagenomic approach was used to decipher the fate of ARGs in 12-year long-term fertilized (inorganic fertilizer, compost manure and a mix of them) acidic, near-neutral and alkaline soils. Read More

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Characterizing the sources, concentrations and resuspension potential of metals and metalloids in the thoracic fraction of urban road dust.

Sci Total Environ 2021 Apr 30;786:147467. Epub 2021 Apr 30.

Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, HECSB, Health Canada, Ottawa, ON, Canada; Earth and Environmental Sciences Department, University of Ottawa, Ottawa, ON, Canada.

Road dust is a sink and source of metals and metalloids of human health concern. To date, many studies have examined the composition of road dust but there remain critical knowledge gaps on the chemistry of thoracic fractions (< 10 μm) and their patterns of deposition and resuspension. The goal of this study is to characterize the elemental concentrations and sources of thoracic fractions of road dust and their resuspension potential for Toronto, Ontario, Canada. Read More

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Discovery and application of dietary compounds to optimize human health, a focus on skeletal muscle regeneration.

Curr Opin Biotechnol 2021 May 7;70:131-135. Epub 2021 May 7.

Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States.

Worldwide, the number of persons over the age of 65 years and those at risk of malnutrition (over and under) is growing, and the prevalence of diet-related chronic disease is at a record high. Pathologies that are linked to poor nutrition underlie the leading causes of death. Safe and effective strategies to improve human health outcomes are urgently required. Read More

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An autoantigen profile of human A549 lung cells reveals viral and host etiologic molecular attributes of autoimmunity in COVID-19.

J Autoimmun 2021 Apr 27;120:102644. Epub 2021 Apr 27.

Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, USA; Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, USA. Electronic address:

We aim to establish a comprehensive COVID-19 autoantigen atlas in order to understand autoimmune diseases caused by SARS-CoV-2 infection. Based on the unique affinity between dermatan sulfate and autoantigens, we identified 348 proteins from human lung A549 cells, of which 198 are known targets of autoantibodies. Comparison with current COVID data identified 291 proteins that are altered at protein or transcript level in SARS-CoV-2 infection, with 191 being known autoantigens. Read More

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Surveillance and molecular characterization of human sapovirus in patients with acute gastroenteritis in Brazil, 2010 to 2017.

J Clin Virol 2021 Apr 26;140:104844. Epub 2021 Apr 26.

Adolfo Lutz Institute, Virology Centre, Av. Dr Arnaldo, no: 355, São Paulo, SP, Brazil. Electronic address:

Background: Human sapoviruses (HuSaV) are associated with acute gastroenteritis (AGE), causing sporadic cases and outbreaks in patients worldwide. In Brazil, however, there are few reports describing the prevalence of HuSaV in patients with AGE.

Objective: Describing the diversity of HuSaV in Brazil by detecting and molecularly characterizing HuSaV among patients with AGE during an 8-year period (2010-2017). Read More

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VDAC1 as a target in cisplatin anti-tumor activity through promoting mitochondria fusion.

Biochem Biophys Res Commun 2021 May 7;560:52-58. Epub 2021 May 7.

Key Laboratory of Molecular Epidemiology of Hunan Province, School of Medicine, Hunan Normal University, Changsha, 410081, PR China. Electronic address:

Cisplatin is one of the most effective anti-cancer drugs, but its efficacy is limited by the development of resistance. Previous studies have shown that mitochondria play critical roles in cisplatin cytotoxicity, however, the exact mechanism of mitochondria involved in cisplatin sensitivity has not been clarified. In this study, cisplatin triggered mitochondrial oxidative stress and the decrease of mitochondria membrane potential in human cervical cancer cells. Read More

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Evaluating the radioprotective effect of Cimetidine, IMOD, and hybrid radioprotectors agents: An in-vitro study.

Appl Radiat Isot 2021 May 6;174:109760. Epub 2021 May 6.

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. Electronic address:

Introduction: There are various radioprotective agents with different mechanisms that help to decrease ionizing radiation side effects. The radioprotective effect of Cimetidine and IMOD was assessed individually and compared with the hybrid radioprotectors agents (HRPAs-IMOD and Cimetidine) on human lymphocyte cells.

Methods: Twenty healthy volunteers (ten men and ten women) participated in the present study. Read More

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Tumstatin attenuates the promotion effect of IL-17 secreted by Th17 cells on the stemness maintenance of glioma cells.

Pathol Res Pract 2021 May 5;223:153463. Epub 2021 May 5.

Department of Neurosurgery, Beilun District People's Hospital of Ningbo City, Ningbo, Zhejiang, 315800, China. Electronic address:

The presence and clinical significance of IL-17 and IL-17-expressing cells have been studied for several cancers, although their correlation with tumor development remains controversial. Peripheral blood was collected from healthy donors and glioma patients to isolate peripheral blood mononuclear cells (PBMCs). The percentage of IL-17-expressing cells and the production of inflammatory cytokines in PBMCs and tissues were measured. Read More

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LPS decreases CFTR open probability and mucociliary transport through generation of reactive oxygen species.

Redox Biol 2021 Apr 30;43:101998. Epub 2021 Apr 30.

Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, AL, USA; Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address:

Lipopolysaccharide (LPS) serves as the interface between gram-negative bacteria (GNB) and the innate immune response in respiratory epithelial cells (REC). Herein, we describe a novel biological role of LPS that permits GNB to persist in the respiratory tract through inducing CFTR and mucociliary dysfunction. LPS reduced cystic fibrosis transmembrane conductance regulater (CFTR)-mediated short-circuit current in mammalian REC in Ussing chambers and nearly abrogated CFTR single channel activity (defined as forskolin-activated Cl currents) in patch clamp studies, effects of which were blocked with toll-like receptor (TLR)-4 inhibitor. Read More

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How should external human-machine interfaces behave? Examining the effects of colour, position, message, activation distance, vehicle yielding, and visual distraction among 1,434 participants.

Appl Ergon 2021 May 7;95:103450. Epub 2021 May 7.

Faculty of Mechanical, Maritime and Materials Engineering, Delft University of Technology, Delft, the Netherlands.

External human-machine interfaces (eHMIs) may be useful for communicating the intention of an automated vehicle (AV) to a pedestrian, but it is unclear which eHMI design is most effective. In a crowdsourced experiment, we examined the effects of (1) colour (red, green, cyan), (2) position (roof, bumper, windshield), (3) message (WALK, DON'T WALK, WILL STOP, WON'T STOP, light bar), (4) activation distance (35 or 50 m from the pedestrian), and (5) the presence of visual distraction in the environment, on pedestrians' perceived safety of crossing the road in front of yielding and non-yielding AVs. Participants (N = 1434) had to press a key when they felt safe to cross while watching a random 40 out of 276 videos of an approaching AV with eHMI. Read More

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Non-conventional water reuse in agriculture: A circular water economy.

Water Res 2021 Apr 26;199:117193. Epub 2021 Apr 26.

Applied Material Division, Argonne National Laboratory, 9700 S. Cass Ave., Argonne, IL 60439, United States.

Due to the growing and diverse demands on water supply, exploitation of non-conventional sources of water has received much attention. Since water consumption for irrigation is the major contributor to total water withdrawal, the utilization of non-conventional sources of water for the purpose of irrigation is critical to assuring the sustainability of water resources. Although numerous studies have been conducted to evaluate and manage non-conventional water sources, little research has reviewed the suitability of available water technologies for improving water quality, so that water reclaimed from non-conventional supplies could be an alternative water resource for irrigation. Read More

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Oversampling of minority categories drives misperceptions of group compositions.

Cognition 2021 May 7;214:104756. Epub 2021 May 7.

Department of Psychology & Center for Cognitive Neuroscience, Duke University, USA.

The ability to estimate proportions informs our immediate impressions of social environments (e.g., of the diversity of races or genders within a crowded room). Read More

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Pyridine-containing octadentate ligand NE3TA-PY for formation of neutral complex with Lu(III) and Y(III) for radiopharmaceutical applications: Synthesis, DFT calculation, radiolabeling, and in vitro complex stability.

J Inorg Biochem 2021 Apr 24;221:111436. Epub 2021 Apr 24.

Department of Chemistry, Lewis College of Science and Letters, Illinois Institute of Technology, Chicago, IL, United States of America.

Targeted radionuclide therapy is a developing therapeutic modality for cancer and employs a cytotoxic radionuclide bound to a chelating agent and a bioactive molecule with high binding affinity for a specific biomarker in tumors. An optimal chelator is one of the critical components to control therapeutic efficacy and toxicity of targeted radionuclide therapy. We designed a new octadentate ligand NE3TA-PY (7-[2-[(carboxymethyl)(2-pyridylmethyl)amino]ethyl]-1,4,7-triazacyclononane-1,4-diacetic acid) for β-particle-emitting Lu and Y with targeted radionuclide therapy applications. Read More

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Tatibody, a recombinant antibody with higher internalization potency.

Mol Immunol 2021 May 7;135:320-328. Epub 2021 May 7.

Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

Using antibody drug conjugates (ADC) which can exclusively bind to their target cells and upon internalization release their toxic agent, is one of the most effective methods for killing tumor cells. Therefore, increasing the internalization rate is an important factor for tumor treatment in this case. The aim of the present study was to develop a new variant of pertuzumab (an anti-ErbB2 humanized antibody) with higher internalization rate that can be a good candidate for the production of ADC. Read More

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Whole transcriptome analysis of schinifoline treatment in Caenorhabditis elegans infected with Candida albicans.

Mol Immunol 2021 May 7;135:312-319. Epub 2021 May 7.

Nanjing Institute for Comprehensive Utilization of Wild Plants, Nanjing, 211100, China. Electronic address:

Candida albicans is an opportunistic fungal human pathogen that has been causing an increasing number of deaths each year. Due to the widespread use of broad-spectrum antibiotics and immunosuppressants, C. albicans resistance to these therapies has increased. Read More

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"Light cannabis" consumption in a sample of young adults: Preliminary pharmacokinetic data and psychomotor impairment evaluation.

Forensic Sci Int 2021 May 2;323:110822. Epub 2021 May 2.

Department of Medical and Surgical Sciences, Unit of Legal Medicine, University of Bologna, Via Irnerio 49, 40126 Bologna, Italy.

Introduction: In 2019, the Italian Supreme Court established that hemp cannot be commercialized for human use, when the "psychotropic effect" of the product or its "offensiveness" can be demonstrated. The aim of the present study is to assess Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) blood concentrations after smoking cannabis with a low percentage of Δ9-THC, also referred as "light cannabis", and its effects on young adults' vigilance, cognitive and motor skills.

Materials And Methods: Eighteen young adults consumed three light cannabis cigarettes containing 400 mg of inflorescences each, with a percentage of 0. Read More

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Executive function as a mechanism linking socioeconomic status to internalizing and externalizing psychopathology in children and adolescents.

J Adolesc 2021 May 7;89:149-160. Epub 2021 May 7.

Department of Psychology, Harvard University, USA.

Introduction: The association between low socioeconomic status (SES) in childhood and increased risk for psychopathology is well established, but the mechanisms explaining this relationship are poorly understood. Here, we investigate the potential role of difficulties in executive functioning (EF) as a mechanism linking childhood and adolescent SES with externalizing and internalizing psychopathology.

Methods: We examined whether difficulties with EF mediated the association between SES and externalizing and internalizing psychopathology in two cross-sectional samples of children and adolescents (Study 1: N = 94, ages 6-18, 51. Read More

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Object affordances from the perspective of an avatar.

Conscious Cogn 2021 May 7;92:103133. Epub 2021 May 7.

Work and Cognitive Psychology, RWTH Aachen University, Germany.

Humans often interact with avatars in video gaming, workplace, or health applications, for instance. The present research studied object affordances from an avatar's perspective. In two experiments, participants responded to objects with a left/right keypress, indicating whether the objects were upright or inverted. Read More

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The association between intended drinking contexts and alcohol expectancies in college students: A daily diary study.

Addict Behav 2021 Apr 30;120:106967. Epub 2021 Apr 30.

Department of Psychiatry and Behavioral Sciences, University of Washington, Center for the Study of Health and Risk Behaviors, 1100 NE 45th St., #300, Seattle, WA 98115, USA.

Background: Because of the role alcohol outcome expectancies play in subsequent drinking, it is important to understand factors that can shape alcohol expectancies to guide intervention efforts. This study examined among college students whether intended social contexts for drinking were associated with positive and negative alcohol expectancies at the daily-level.

Methods: Participants included in analyses were 323 students, ages 18 to 24 years, enrolled at a 4-year university in the Pacific Northwest. Read More

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Chlorogenic acid is a positive regulator of MDA5, TLR7 and NF-κB signaling pathways mediated antiviral responses against Gammacoronavirus infection.

Int Immunopharmacol 2021 May 7;96:107671. Epub 2021 May 7.

Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address:

Chlorogenic acid (CGA) is a phenolic compound that has been well studied for its antiviral, anti-inflammatory and immune stimulating properties. This research was aimed to focus on the antiviral properties of CGA on infectious bronchitis virus (IBV) in vivo and in vitro for the very first time. The outcome of in vitro experiments validated that, out of five previously reported antiviral components, CGA significantly reduced the relative mRNA expression of IBV-N in CEK cells. Read More

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CircATRNL1 protects against osteoarthritis by targeting miR-153-3p and KLF5.

Int Immunopharmacol 2021 May 7;96:107704. Epub 2021 May 7.

Department of Orthopaedics, the 1st Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address:

Background: Osteoarthritis (OA) is characterized by chondrocyte injury. Circular RNAs (circRNAs) are involved in the pathogenesis of various diseases, including OA. The purpose of this study was to determine the potential role of circATRNL1 in OA pathology in vitro. Read More

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Growth hormone replacement may influence the biological action of thyroid hormone on liver and bone tissue.

Growth Horm IGF Res 2021 Apr 30;57-58:101393. Epub 2021 Apr 30.

Department of Endocrinology, Beaumont Hospital, Dublin, Ireland. Electronic address:

Objective: Growth hormone (GH) replacement alters the peripheral interconversion of thyroxine (T4) and triiodothyronine (T3). However, little is known about the clinical impact of these alterations. We aimed to compare changes observed in the serum T3:T4 ratio with known biological markers of thyroid hormone action derived from different peripheral tissues. Read More

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