129,393 results match your criteria human complement


Disease type detection in lung and colon cancer images using the complement approach of inefficient sets.

Authors:
Mesut Toğaçar

Comput Biol Med 2021 Sep 2;137:104827. Epub 2021 Sep 2.

Department of Computer Technology, Technical Sciences Vocational School, Fırat UniversityElazig, Turkey. Electronic address:

Lung and colon cancers are deadly diseases that can develop simultaneously in organs and adversely affect human life in some special cases. Although the frequency of simultaneous occurrence of these two types of cancer is unlikely, there is a high probability of metastasis between the two organs if not diagnosed early. Traditionally, specialists have to go through a lengthy and complicated process to examine histopathological images and diagnose cancer cases; yet, it is now possible to achieve this process faster with the available technological possibilities. Read More

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September 2021

Effects of early pregnancy on the complement system in the ovine thymus.

Vet Res Commun 2021 Sep 24. Epub 2021 Sep 24.

Department of Animal Science, School of Life Sciences and Food Engineering, Hebei University of Engineering, No. 19 Taiji Road, Handan, 056038, China.

The complement system is crucial for the innate immune system, and complement activation is related to abnormal pregnancy in mice and humans. It is hypothesized that the complement system participates in maternal thymic immune regulation during early pregnancy in sheep. In this study, maternal thymuses were sampled on day 16 of the estrous cycle, and days 13, 16 and 25 of gestation in sheep. Read More

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September 2021

In vitro antioxidant activity of crude extracts of Harpagophytum zeyheri and their anti-inflammatory and cytotoxicity activity compared with diclofenac.

BMC Complement Med Ther 2021 Sep 23;21(1):238. Epub 2021 Sep 23.

Department of Applied Chemistry, Faculty of Applied Science, National University of Science and Technology, P. O. Box AC 939, Ascot, Bulawayo, Zimbabwe.

Background: This study evaluated the in vitro antioxidant activity and comparison of anti-inflammatory and cytotoxic activity of Harpagopytum zeyheri with diclofenac.

Methods: In vitro assays were conducted using water, ethanol, and ethyl acetate extracts of H.zeyheri. Read More

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September 2021

Using sound to understand protein sequence data: new sonification algorithms for protein sequences and multiple sequence alignments.

BMC Bioinformatics 2021 Sep 23;22(1):456. Epub 2021 Sep 23.

Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Charlotte Auerbach Road, The King's Buildings, Edinburgh, EH9 3FL, UK.

Background: The use of sound to represent sequence data-sonification-has great potential as an alternative and complement to visual representation, exploiting features of human psychoacoustic intuitions to convey nuance more effectively. We have created five parameter-mapping sonification algorithms that aim to improve knowledge discovery from protein sequences and small protein multiple sequence alignments. For two of these algorithms, we investigated their effectiveness at conveying information. Read More

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September 2021

Systematic analysis of chemical-protein interactions from zebrafish embryo by proteome-wide thermal shift assay, bridging the gap between molecular interactions and toxicity pathways.

J Proteomics 2021 Sep 20:104382. Epub 2021 Sep 20.

Department of Biomedical and Clinical Sciences, Department of Cell Biology, Faculty of Medicine, Linköping University, Linköping, Sweden; Ikerbasque, Basque Foundation for Sciences, Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Spain. Electronic address:

The molecular interaction between chemicals and proteins often promotes alteration of cellular function. One of the challenges of the toxicology is to predict the impact of exposure to chemicals. Assessing the impact of exposure implies to understand their mechanism of actions starting from identification of specific protein targets of the interaction. Read More

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September 2021

Biologia Futura: stories about the functions of β-integrins in human phagocytes.

Authors:
Zsuzsa Bajtay

Biol Futur 2021 Mar 6;72(1):7-13. Epub 2021 Feb 6.

Institute of Biology, Department of Immunology and MTA-ELTE Immunology Research Group, Eötvös Loránd University, Pázmány P. s. 1/C, Budapest, H-1117, Hungary.

Integrins are essential membrane proteins that provide a tightly regulated link between the extracellular matrix and the intracellular cytoskeletal network. These cell surface proteins are composed of a non-covalently bound α chain and β chain. The leukocyte-specific complement receptor 3 (CR3, αβ, CD11b/CD18) and complement receptor 4 (CR4, αβ, CD11c/CD18) belong to the family of β-integrins. Read More

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A small regulatory RNA alters Staphylococcus aureus virulence by titrating RNAIII activity.

Nucleic Acids Res 2021 Sep 23. Epub 2021 Sep 23.

Inserm, BRM [Bacterial Regulatory RNAs and Medicine] - UMR_S 1230, 35033 Rennes, France.

Staphylococcus aureus is an opportunistic human and animal pathogen with an arsenal of virulence factors that are tightly regulated during bacterial infection. The latter is achieved through a sophisticated network of regulatory proteins and regulatory RNAs. Here, we describe the involvement of a novel prophage-carried small regulatory S. Read More

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September 2021

Biomimetic microcavity interfaces for a label-free capture of pathogens in the fluid bloodstream by vortical crossflow filtration.

Nanoscale 2021 Sep 23;13(36):15220-15230. Epub 2021 Sep 23.

Joint Centre of Translational Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.

Bacterial sepsis is a lethal disease triggered by microbial pathogens. The blood pathogen load is a major contributor to both disease severity and mortality in patients with sepsis blood. Therefore, it is crucial to reduce the load of pathogens, in particular the drug-resistant pathogens. Read More

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September 2021

Increased activation product of complement 4 protein in plasma of individuals with schizophrenia.

Transl Psychiatry 2021 Sep 22;11(1):486. Epub 2021 Sep 22.

Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, 94305, USA.

Structural variation in the complement 4 gene (C4) confers genetic risk for schizophrenia. The variation includes numbers of the increased C4A copy number, which predicts increased C4A mRNA expression. C4-anaphylatoxin (C4-ana) is a C4 protein fragment released upon C4 protein activation that has the potential to change the blood-brain barrier (BBB). Read More

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September 2021

Periphery kinetic perimetry: clinically feasible to complement central static perimetry.

BMC Ophthalmol 2021 Sep 23;21(1):343. Epub 2021 Sep 23.

Department of Ophthalmology, Chengdu First People's Hospital, Chengdu, Sichuan, China.

Background: Existing evidence suggests that visual field defect in eyes with glaucoma significantly varies between individuals. The following study compared the central visual field defects with the peripheral visual field defects in patients with suspect glaucoma and primary open-angle glaucoma (POAG) and investigated whether using the central visual field test alone could result in loss of clinically valuable information.

Methods: In this prospective observational study, 167 eyes from 89 patients with suspect glaucoma or POAG were first examined with static automated perimetry (SAP), followed by a peripheral visual field test on Octopus 900 perimeter (Haag-Streit, Koeniz, Switzerland). Read More

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September 2021

Vesicular formation regulated by ERK/MAPK pathway mediates human erythroblast enucleation.

Blood Adv 2021 Sep 22. Epub 2021 Sep 22.

New York Blood Center, NEw York, New York, United States.

Enucleation is a key event in mammalian erythropoiesis responsible for generation of enucleated reticulocytes. While progress is being made in developing mechanistic understanding of enucleation, our understanding of mechanisms for enucleation is still incomplete. Mitogen-activated protein kinase (MAPK) pathway plays diverse roles in biological processes but its role in erythropoiesis is yet to be fully defined. Read More

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September 2021

Potential natural products that target the SARS-CoV-2 spike protein identified by structure-based virtual screening, isothermal titration calorimetry and lentivirus particles pseudotyped (Vpp) infection assay.

J Tradit Complement Med 2021 Sep 16. Epub 2021 Sep 16.

Chinese Medicine Research and Development Center, Sex Hormone Research Center, Department of Obstetrics and Gynecology, Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan.

Background And Aim: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters cells through the binding of the viral spike protein with human angiotensin-converting enzyme 2 (ACE2), resulting in the development of coronavirus disease 2019 (COVID-19). To date, few antiviral drugs are available that can effectively block viral infection. This study aimed to identify potential natural products from Taiwan Database of Extracts and Compounds (TDEC) that may prevent the binding of viral spike proteins with human ACE2 proteins. Read More

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September 2021

Genomic and Phenotypic Characteristics for Infections.

Infect Drug Resist 2021 14;14:3721-3726. Epub 2021 Sep 14.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.

Background: (VV) is a causative agent of foodborne diseases with high mortality. The aim of this study was to investigate the genomic and phenotypic profiles of VV.

Methods: Six VV isolates were collected and conducted whole-genome sequencing. Read More

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September 2021

Soy Foods and the Risk of Fracture: A Systematic Review of Prospective Cohort Studies.

Complement Med Res 2021 Sep 21:1-10. Epub 2021 Sep 21.

Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Objectives: The primary objective of our study was to systematically review all available prospective cohort studies which investigated the association of soy food intake and incident fracture risk.

Methods: We searched PubMed, Scopus, and Embase databases for relevant studies up to June 2021.

Synthesis: Of 695 records, a total of 5 cohort studies were included in the current systematic review. Read More

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September 2021

AEOL 10150 Alleviates Radiation-induced Innate Immune Responses in Non-human Primate Lung Tissue.

Health Phys 2021 Oct;121(4):331-344

Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD 21201.

Abstract: To study the molecular and cellular mechanisms of radiation-induced lung injury (RILI) in a non-human primate model, Rhesus macaques were irradiated with lethal doses of radiation to the whole thorax. A subset of the irradiated animals was treated with AEOL 10150, a potent catalytic scavenger of reactive oxygen and nitrogen species. Lung tissues were collected at necropsy for molecular and immunohistochemical (IHC) studies. Read More

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October 2021

Balanced Solvent Model for Intrinsically Disordered and Ordered Proteins.

J Chem Inf Model 2021 Sep 21. Epub 2021 Sep 21.

State Key Laboratory of Microbial metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, Department of Bioinformatics and Biostatistics, National Experimental Teaching Center for Life Sciences and Biotechnology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Intrinsically disordered proteins (IDPs) have no fixed three-dimensional (3D) structures under physiological conditions, with the content being about 51% in human proteomics. IDPs are associated with many human diseases, such as cancer, diabetes, and neurodegenerative diseases. Because IDPs do not crystallize and have diverse conformers, traditional experimental methods such as crystallization and NMR can hardly capture their conformation ensemble and just provide average structural characters of IDPs. Read More

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September 2021

Design of an Interactive Web Application for Teaching Uncertainty Interpretations of Clinical Tests.

Stud Health Technol Inform 2021 Sep;283:156-162

Chair of Medical Informatics, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Erlangen, Germany.

Background: Assessing the uncertainty of diagnostic findings is essential for advising patients. Previous research has demonstrated the difficulty of computing the expected correctness of positive or negative results, although clinical decision support (CDS) tools promise to facilitate adequate interpretations.

Objectives: To teach the potential utility of CDS tools to medical students, we designed an interactive software module that computes and visualizes relevant probabilities from typical inputs. Read More

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September 2021

Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma.

Cancer Immunol Immunother 2021 Sep 20. Epub 2021 Sep 20.

Division of Hematopoiesis, Joint Research Center for Human Retrovirus Infection, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto, 860-0811, Japan.

Primary effusion lymphoma (PEL) is a rare, aggressive B cell non-Hodgkin's lymphoma of the body cavities with malignant effusions. The prognosis is poor, and no optimal treatment has been established. CD38 is a type II transmembrane glycoprotein known to overexpress in multiple myeloma (MM). Read More

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September 2021

Netherton syndrome subtypes share IL-17/IL-36 signature with distinct IFN-α and allergic responses.

J Allergy Clin Immunol 2021 Sep 17. Epub 2021 Sep 17.

INSERM UMR 1163, Laboratory of Genetic Skin Diseases, Imagine Institute, 75015 Paris, France; University of Paris, 75006 Paris, France; Department of Genetics, Necker Hospital for sick children (AP-HP), 75015 Paris, France. Electronic address:

Background: Netherton syndrome (NS) is a rare recessive skin disorder caused by loss-of-function mutations in SPINK5 encoding the protease inhibitor LEKTI. NS patients suffer from a severe skin barrier defect, display inflammatory skin lesions and superficial scaling with atopic manifestations. They present with typical ichthyosis linearis circumflexa (NS-ILC) or scaly erythroderma (NS-SE). Read More

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September 2021

Subject-Specific Spino-Pelvic Models Reliably Measure Spinal Kinematics During Seated Forward Bending in Adult Spinal Deformity.

Front Bioeng Biotechnol 2021 1;9:720060. Epub 2021 Sep 1.

Department of Development and Regeneration, Faculty of Medicine, Institute for Orthopaedic Research and Training (IORT), KU Leuven, Leuven, Belgium.

Image-based subject-specific models and simulations are recently being introduced to complement current state-of-the-art mostly static insights of the adult spinal deformity (ASD) pathology and improve the often poor surgical outcomes. Although the accuracy of a recently developed subject-specific modeling and simulation framework has already been quantified, its reliability to perform marker-driven kinematic analyses has not yet been investigated. The aim of this work was to evaluate the reliability of this subject-specific framework to measure spine kinematics in ASD patients, in terms of 1) the overall test-retest repeatability; 2) the inter-operator agreement of spine kinematic estimates; and, 3) the uncertainty of those spine kinematics to operator-dependent parameters of the framework. Read More

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September 2021

Osthole Induces Apoptosis and Inhibits Proliferation, Invasion, and Migration of Human Cervical Carcinoma HeLa Cells.

Evid Based Complement Alternat Med 2021 9;2021:8885093. Epub 2021 Sep 9.

Henan University of Chinese Medicine, No. 156 Jinshui East Road, Zhengzhou, Henan 450046, China.

Purpose: To study the effect of osthole extract on proliferation, migration, invasion, and apoptosis of human cervical carcinoma HeLa cells and investigate its underlying mechanism.

Methods: HeLa cells were exposed to osthole at various concentrations. Cell viability, migration, and invasion were detected by MTT assay, scratch wound-healing assay, and invasion assay, respectively. Read More

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September 2021

Serum Complement Activation by C4BP-IgM Fusion Protein Can Restore Susceptibility to Antibiotics in .

Front Immunol 2021 1;12:726801. Epub 2021 Sep 1.

Department of Translational Medicine, Lund University, Malmö, Sweden.

is the etiological agent of gonorrhea, the second most common bacterial sexually transmitted infection worldwide. Reproductive sequelae of gonorrhea include infertility, ectopic pregnancy and chronic pelvic pain. Most antibiotics currently in clinical use have been rendered ineffective due to the rapid spread of antimicrobial resistance among gonococci. Read More

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September 2021

Fast Optical Investigation of Cardiac Electrophysiology by Parallel Detection in Multiwell Plates.

Front Physiol 2021 3;12:692496. Epub 2021 Sep 3.

European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, Italy.

Current techniques for fast characterization of cardiac electrophysiology employ optical technologies to control and monitor action potential features of single cells or cellular monolayers placed in multiwell plates. High-speed investigation capacities are commonly achieved by serially analyzing well after well employing fully automated fluorescence microscopes. Here, we describe an alternative cost-effective optical approach (MULTIPLE) that exploits high-power LED arrays to globally illuminate a culture plate and an sCMOS sensor for parallel detection of the fluorescence coming from multiple wells. Read More

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September 2021

Human versus chatbot: Understanding the role of emotion in health marketing communication for vaccines.

Psychol Mark 2021 Jul 27. Epub 2021 Jul 27.

Department of Interactive Media University of Miami Miami Florida USA.

Based on the theoretical framework of agency effect, this study examined the role of affect in influencing the effects of chatbot versus human brand representatives in the context of health marketing communication about HPV vaccines. We conducted a 2 (perceived agency: chatbot vs. human) × 3 (affect elicitation: embarrassment, anger, neutral) between-subject lab experiment with 142 participants, who were randomly assigned to interact with either a perceived chatbot or a human representative. Read More

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Editorial: Autoantibodies to Components of the Immune System, Including Type 1 Interferons, and the Risk of Severe COVID-19.

Authors:
Dinah V Parums

Med Sci Monit 2021 Sep 20;27:e934766. Epub 2021 Sep 20.

Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA.

During the past two years, clinical studies have attempted to identify risk factors to predict clinical outcomes following infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In July 2021, a study using a high-throughput technique detected autoantibodies to chemokines, cytokines, and complement components in patients with symptomatic coronavirus disease 2019 (COVID-19). In August 2021, a study identified pre-existing autoantibodies to type 1 interferons (IFNs) in 10% of patients with severe COVID-19 but not asymptomatic individuals. Read More

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September 2021

BL-01, an Fc-bearing, tetravalent CD20 × CD5 bispecific antibody, redirects multiple immune cells to kill tumors in vitro and in vivo.

Cytotherapy 2021 Sep 17. Epub 2021 Sep 17.

Division of Hematology, Center of Cellular Therapy "G. Lanzani," Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy; Fondazione per la Ricerca Ospedale Maggiore, Bergamo, Italy. Electronic address:

Background Aims: The authors describe here a novel therapeutic strategy combining a bispecific antibody (bsAb) with cytokine-induced killer (CIK) cells.

Methods: The authors have designed, produced and purified a novel tetravalent IgG1-like CD20 × CD5 bsAb called BL-01. The bsAb is composed of a fused heavy chain and two free light chains that pair correctly to the heavy chain sequences thanks to complementary mutations in the monoclonal antibody 2 CH1/CL sequences. Read More

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September 2021

C-reactive protein protects against acetaminophen-induced liver injury by preventing complement overactivation.

Cell Mol Gastroenterol Hepatol 2021 Sep 15. Epub 2021 Sep 15.

MOE Key Laboratory of Environment and Genes Related to Diseases, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China; Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province, Xi'an Children's Hospital, Xi'an Jiaotong University, Xi'an, P.R. China. Electronic address:

Background And Aims: C-reactive protein (CRP) is a hepatocyte-produced marker of inflammation yet with undefined function in liver injury. We aimed to examine the role of CRP in acetaminophen-induced liver injury (AILI).

Methods: The effects of CRP in AILI were investigated using CRP knockout mice and rats combined with human CRP rescue. Read More

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September 2021

DDX3X and DDX3Y are redundant in protein synthesis.

RNA 2021 Sep 17. Epub 2021 Sep 17.

University of California, San Francisco;

DDX3 is a DEAD-box RNA helicase that regulates translation and is encoded by the X- and Y-linked paralogs DDX3X and DDX3Y. While DDX3X is ubiquitously expressed in human tissues and essential for viability, DDX3Y is male-specific and shows lower and more variable expression than DDX3X in somatic tissues. Heterozygous genetic lesions in DDX3X mediate a class of developmental disorders called DDX3X syndrome, while loss of DDX3Y is implicated in male infertility. Read More

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September 2021

Comparison of Efficacy and Safety of Recombinant Human Prourokinase and Alteplase in the Treatment of STEMI and Analysis of Influencing Factors of Efficacy.

Evid Based Complement Alternat Med 2021 6;2021:6702965. Epub 2021 Sep 6.

The Affiliated Nanhua Hospital, Department of Cardiology, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.

Objective: To compare the efficacy and safety of recombinant human prourokinase (rhPro-UK) and alteplase for thrombolytic therapy in acute ST-segment elevation myocardial infarction (STEMI) and to analyze the related factors affecting efficacy.

Methods: From January 2017 to December 2019, 100 patients diagnosed with STEMI were selected and randomly divided into the control group ( = 50) and the observation group ( = 50). Based on conventional treatments, the control group was treated with alteplase, and the observation group was treated with rhPro-UK, and both were treated for 7 days. Read More

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September 2021

Repetitive Transcranial Magnetic Stimulation Improves Neuropathy and Oxidative Stress Levels in Rats with Experimental Cerebral Infarction through the Nrf2 Signaling Pathway.

Evid Based Complement Alternat Med 2021 6;2021:3908677. Epub 2021 Sep 6.

The National Clinical Research Center for Mental Disorders & Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Capital Medical University, Beijing 100088, China.

Ischemic stroke poses a serious threat to human health. Its high morbidity, disability, and lethality rates have led to it being a research hotspot. Cerebral ischemia reperfusion injury is a difficult point in the treatment of ischemic stroke. Read More

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September 2021