68 results match your criteria hu-ab


N-Terminal Extensions Appear to Frustrate HU Heterodimer Formation by Strengthening Intersubunit Contacts and Blocking the Formation of a Heterotetrameric Intermediate.

Biochemistry 2021 Jun 21;60(23):1836-1852. Epub 2021 May 21.

Centre for Protein Science, Design and Engineering (CPSDE), Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector-81, SAS Nagar, Punjab 140306, India.

HU is a bacterial nucleoid-associated protein. Two homologues, known as HU-A, and HU-B, are found in within which the early, late, and stationary phases of growth are dominated by HU-AA, HU-BB, and HU-AB dimers, respectively. Here, using genetic manipulation, mass spectrometry, spectroscopy, chromatography, and electrophoretic examination of glutaraldehyde-mediated cross-linking of subunits, in combination with experiments involving mixing, co-expression, unfolding, and refolding of HU chains, we show that the spontaneous formation of HU-AB heterodimers that is reported to occur upon mixing of wild-type HU-AA and HU-BB homodimers does not occur if chains possess N-terminal extensions. Read More

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HU-AB simulacrum: Fusion of HU-B and HU-A into HU-B-A, a functional analog of the Escherichia coli HU-AB heterodimer.

Biochem Biophys Res Commun 2021 Jun 5;560:27-31. Epub 2021 May 5.

Centre for Protein Science, Design and Engineering (CPSDE), Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector-81, SAS Nagar, Punjab, 140306, India. Electronic address:

In enteric bacteria such as Escherichia coli, there are two homologs of the DNA-binding nucleoid associated protein (NAP) known as HU. The two homologs are known as HU-A and HU-B, and exist either in the form of homodimers (HU-AA, or HU-BB) or as heterodimers (HU-AB), with different propensities to form higher-order oligomers. The three different dimeric forms dominate different stages of bacterial growth, with the HU-AB heterodimer dominating cultures in the stationary phase. Read More

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Yield of Random Biopsies During Colonoscopies in Inflammatory Bowel Disease Patients Undergoing Dysplasia Surveillance.

Inflamm Bowel Dis 2021 May;27(6):779-786

Division of Gastroenterology, Massachusetts General Hospital, Boston, MA, USA.

Background: The development of chromoendoscopy (CE) and high definition endoscopy (HDE) has improved detection of subtle colonic dysplasia in patients with inflammatory bowel diseases (IBDs). The role of random biopsies for dysplasia surveillance is unclear.

Methods: We reviewed patients with IBD who underwent a CE or HDE colonoscopy and had colonic dysplasia detected. Read More

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[Early efficacy of islet transplantation in the treatment of adult advanced diabetes].

Zhonghua Yi Xue Za Zhi 2020 Jul;100(26):2040-2043

Organ Transplantation Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

To evaluate the safety and efficacy of islet transplantation for patients with advanced diabetes. Five cases of islet allotransplantation were performed on 4 adult recipients. The same blood type adult brain-dead pancreas donors were selected and the islets were prepared in GMP laboratory. Read More

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Ileal or Colonic Histologic Activity Is Not Associated With Clinical Relapse in Patients With Crohn's Disease in Endoscopic Remission.

Clin Gastroenterol Hepatol 2021 Jun 29;19(6):1226-1233.e1. Epub 2020 Apr 29.

Division of Gastroenterology, Massachusetts General Hospital, Boston, Massachusetts. Electronic address:

Background & Aims: Goals of treatment for Crohn's disease (CD) are clinical and endoscopic remission. It is not clear whether histologic markers of healing associate with endoscopic remission in patients with CD.

Methods: We identified patients with CD from a single institutional registry, and collected data from 129 patients (46. Read More

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Novel surgical technique and efficacy analysis of donor pancreas preparation without vascular reconstruction in pancreas transplantation.

J Int Med Res 2019 Dec 10;47(12):6182-6191. Epub 2019 Sep 10.

Organ Transplantation Center of the First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation Center), Guangzhou, China.

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December 2019

[Effect of Wnt/β-catenin signal regulating EMT level on the differentiation of mouse ESC to liver tissue].

Zhonghua Yi Xue Za Zhi 2018 Aug;98(30):2441-2447

Organ Transplantation Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

To study embryonic stem cell (ESC) differentiation into liver tissue structure from the perspective of epithelial mesenchymal transition (EMT). ESC of Balb/c mice was selected to induced into hepatic cell using hepatocyte growth factor (HGF), fibroblast growth factor (FGF) and at the time points of metaphase (13 d) and maturity (17 d) of differentiation, dynamic inhibition of Wnt/β-catenin signal was made to reduce the level of EMT. Finally, three-dimensional organization structure growth of the differentiation cells was observed in the differentiation system. Read More

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A simplified multivisceral transplantation procedure for patients with combined end-stage liver disease and type 2 diabetes mellitus.

Liver Transpl 2017 09;23(9):1161-1170

Organ Transplant Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

In liver transplant patients with type 2 diabetes mellitus (DM), the disease worsens after transplantation because of longterm use of diabetogenic immunosuppressive drugs, making management of those patients a great challenge. The objective of our study was to evaluate the safety and efficacy of a simplified multivisceral transplantation (SMT) procedure for the treatment of patients with end-stage liver disease and concurrent type 2 DM. Forty-four patients who had pretransplant type 2 DM were included. Read More

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September 2017

Neurological Autoantibody Prevalence in Epilepsy of Unknown Etiology.

JAMA Neurol 2017 04;74(4):397-402

Department of Neurology and Neurotherapeutics, UT Southwestern Medical Center, Dallas, Texas2Department of Neurology, Parkland Health and Hospital System, Dallas, Texas.

Importance: Autoimmune epilepsy is an underrecognized condition, and its true incidence is unknown. Identifying patients with an underlying autoimmune origin is critical because these patients' condition may remain refractory to conventional antiseizure medications but may respond to immunotherapy.

Objective: To determine the prevalence of neurological autoantibodies (Abs) among adult patients with epilepsy of unknown etiology. Read More

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[The effect of semimature dendritic cell and the levels of Treg on transplantation tolerance of hepatocytes differentiated from mouse embryonic stem cell].

Zhonghua Yi Xue Za Zhi 2017 Jan;97(4):259-264

The First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), Guangzhou 510080, China.

To investigate the inducing effect and mechanism of semimature dendritic cell (smDCs) on transplantation tolerance of hepatocytes differentiated from mouse embryonic stem cells (ESCs), and to study the connections between smDCs and regulatory dendritic cells (regDCs). ESCs of 129 mouse labelled green fluorescent protein (GFP) were induced to hepatocytes by using previous methods. Meanwhile, bone marrow mononuclear cells of 129 mouse were induced to smDCs and regDCs. Read More

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January 2017

Accuracy of FDG-PET/CT and paraneoplastic antibodies in diagnosing cancer in paraneoplastic neurological syndromes.

Rev Esp Med Nucl Imagen Mol 2016 Jan-Feb;35(1):17-21. Epub 2015 Aug 8.

Istanbul University, Istanbul Faculty of Medicine, Department of Nuclear Medicine, Istanbul, Turkey.

Objective: There is still no consensus about whether to perform PET/CT to detect carcinoma in paraneoplastic neurological syndromes (PNS) in patients with or without antibodies. The aim of this study is to determine the diagnostic accuracy of PET/CT and antibodies in patients with PNS.

Material And Methods: A retrospective study was conducted on patients with clinically suspected PNS between 2008 and 2013. Read More

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Imbalance in circulating T lymphocyte subsets contributes to Hu antibody-associated paraneoplastic neurological syndromes.

Cell Immunol 2014 Aug 14;290(2):245-50. Epub 2014 Jul 14.

Department of Immunology, and An Hui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu A233030, China.

Paraneoplastic neurological syndromes (PNS) are associated with small cell lung cancer (SCLC) and Hu antibodies, which are considered to have an immune-mediated etiology. As a pathogenic role for Hu antibodies (Hu-Ab) in PNS could not be demonstrated, the cellular immune response against the Hu proteins has been further investigated. To delve deeper into the hypothesized cell-mediated immune pathogenesis of these syndromes, imbalances within circulating T lymphocyte subsets were investigated to determine their significance in Hu antibody-associated PNS. Read More

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Liver transplantation using organs from deceased organ donors: a single organ transplant center experience.

Hepatobiliary Pancreat Dis Int 2014 Aug;13(4):409-15

Organ Transplant Center, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.

Background: In 2011, a pilot program for deceased organ donation was initiated in China. We describe the first successful series of liver transplants in the pilot program.

Methods: From July 2011 to August 2012, our center performed 26 liver transplants from a pool of 29 deceased donors. Read More

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Radiofrequency-assisted versus clamp-crush liver resection: a systematic review and meta-analysis.

J Surg Res 2014 Apr 31;187(2):471-83. Epub 2013 Oct 31.

Department of Hepatobiliary Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address:

Background: Conflicting results were found between radiofrequency-assisted liver resection (RF-LR) and clamp-crush liver resection (CC-LR) during liver surgery. We conducted a systematic review and meta-analysis that included randomized controlled trials (RCTs) and non-RCTs to compare the effectiveness and safety of RF-LR versus CC-LR during liver surgery.

Methods: Articles comparing RF-LR and CC-LR that were published before December 2012 were retrieved and subjected to a systematic review and meta-analysis. Read More

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Antiapoptotic gene BAG-1 vector structure of RNA interference and endogenous targeted screening in colon cancer cell lines.

Tumour Biol 2014 Feb 6;35(2):1057-63. Epub 2013 Sep 6.

Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 250012, China.

The purposes of the present work were to construct the shRNA plasmids for BAG-1 gene of human and test the expression of mRNA and protein of BAG-1. Recombinant plasmids containing green fluorescent protein reporter genes are constructed using gene cloning methods. The shRNA plasmids for the BAG-1 gene are constructed by RNA interference technology. Read More

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February 2014

Value of ¹⁸F-FDG-PET/CT in the detection of recurrent hepatocellular carcinoma after hepatectomy or radiofrequency ablation: a comparative study with contrast-enhanced ultrasound.

J Dig Dis 2013 Aug;14(8):433-8

Department of Nuclear Medicine, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Objective: To evaluate the role of positron emission tomography/computer tomography with fluorine-18 fluorodeoxyglucose ((18) F-FDG-PET/CT) in detecting hepatocellular carcinoma (HCC) recurrence after hepatectomy and/or radiofrequency ablation (RFA) and to compare its efficacy with contrast-enhanced ultrasound (CEUS).

Methods: A total of 36 HCC patients were included in this study. All patients underwent both (18) F-FDG-PET/CT and CEUS at least once for the diagnosis of HCC recurrence. Read More

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Antibody repertoire in paraneoplastic cerebellar degeneration and small cell lung cancer.

PLoS One 2013 25;8(3):e60438. Epub 2013 Mar 25.

Service of Neurology, Hospital Clínic, Universitat de Barcelona and Institut d´Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain.

The goal of this study is to determine whether patients with paraneoplastic cerebellar degeneration (PCD) and small-cell lung cancer (SCLC) have a specific repertoire of antibodies, if SOX1 antibodies (SOX1-ab) can predict the presence of SCLC, and if antibodies to cell surface antigens occur in this syndrome. Antibody analysis was done using immunohistochemistry on rat brain, immunoblot with recombinant antigens, screening of cDNA expression libraries, and immunolabeling of live neurons in 39 patients with PCD and SCLC. VGCC-ab were measured by RIA, and SOX1-ab, Hu-ab, and ZIC4-ab by immunoblot. Read More

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December 2013

[Roles of E-cadherin in enhancing the in vitro differentiation of hepatocytes from murine embryonic stem cells].

Zhonghua Yi Xue Za Zhi 2012 Nov;92(44):3143-6

Organ Transplantation Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

Objective: To investigate the roles of E-cadherin (E-cad) in enhancing the in vitro differentiation of hepatocytes from murine embryonic stem cells (ESCs).

Methods: Exogenous E-cad was transfected into BALB/c murine ESCs to enable its stable expression. Then hepatic differentiation from E-cad-ESCs was induced by such growth factors as hepatocyte growth factor (HGF), fibroblast growth factor (FGF) and transforming growth factor (TGF). Read More

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November 2012

Safety and efficacy of four steroid-minimization protocols in liver transplant recipients: 3-year follow-up in a single center.

J Dig Dis 2013 Jan;14(1):38-44

Department of General Surgery, Organ Transplantation Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Objective: To evaluate the safety and efficacy of steroid-minimization therapy in liver transplantation (LT) recipients with hepatitis B virus-related diseases in China.

Methods: From March 2000 to June 2007, 502 adult LT recipients, mostly with hepatitis B (HBV)-related diseases, were enrolled in our study. Four study groups were setup according to the steroid-minimization protocols: tacrolimus (TAC) with 6 months steroids withdrawal (6M SW), TAC with 3 months SW (3M SW), TAC with 14 days SW (14d SW), and TAC with basiliximab induction and steroids avoidance (Bas SA). Read More

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January 2013

[Autoimmune encephalitis, clinical, radiological and immunological data].

Rev Neurol (Paris) 2013 Feb 23;169(2):142-53. Epub 2012 Oct 23.

Département de neurologie, CHU de Strasbourg, 3, avenue Molière, BP 426, 67091 Strasbourg cedex, France.

Introduction: Encephalitis is an inflammatory or infectious disease with an acute or subacute presentation. Immunological abnormalities in serum can be found but may be underdiagnosed. In several cases, a paraneoplastic origin with anti-neuron antibodies is noted. Read More

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February 2013

Preparation and characterization of nano-liposome-mediated FL gene in the Lovo cells.

Cancer Biother Radiopharm 2012 Oct 18;27(8):490-4. Epub 2012 Sep 18.

Department of General Surgery, Qilu Hospital of Shandong University , Jinan, China.

Aims: The purpose of the present work was to formulate and evaluate cationic nano-liposomes as novel nonviral gene delivery for colon cancer treatment.

Methods: Recombinant pEGFP-c1-Fms-like tyrosine kinase receptor 3 ligand (FL) plasmids containing human FL gene and green fluorescent protein (GFP) reporter genes were constructed. FL and GFP Gene-carrying cationic nano-liposomes were prepared based on the electrostatic adherence principle and then transfected into Lovo cells. Read More

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October 2012

[Efficacy and safety of sorafenib in the prevention and treatment of hepatocellular carcinoma recurrences after liver transplantation].

Zhonghua Yi Xue Za Zhi 2012 May;92(18):1264-7

Organ Transplantation Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

Objective: To evaluate the efficacy and safety of sorafenib in the prevention and treatment of hepatocellular carcinoma (HCC) relapse after liver transplantation.

Methods: A retrospective cohort study was performed to assess the efficacy and safety of sorafenib for HCC. Forty-four patients who underwent liver transplant for HCC beyond Milan criteria form July 2007 to May 2010 were included study group (sorafenib, n = 22) and control group (without sorafenib, n = 22). Read More

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[Clinical characteristics, diagnosis and treatment of digestive tract leakage after orthotopic liver transplantation].

Zhonghua Wai Ke Za Zhi 2012 Mar;50(3):222-5

Organ Transplantation Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.

Objective: To investigate the clinical characteristics, diagnosis and treatment of digestive tract leakage after orthotopic liver transplantation (OLT).

Methods: Sixty-one recipients had digestive tract leakage in early stage after OLT among 1173 cases from January 2000 to December 2010. There were 55 male and 6 female patients, aging from 36 to 61 years, with a median of 45 years. Read More

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An atomic-force basis for the bacteriolytic effects of granulysin.

Colloids Surf B Biointerfaces 2012 Dec 18;100:163-8. Epub 2012 May 18.

Department of Chemistry, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.

While granulysin has been suggested to play an important role in adaptive immune responses against bacterial infections by killing pathogens, and molecular force for protein-protein interaction or protein-bacteria interaction may designate the specific functions of a protein, the molecular-force basis underlying the bacteriolytic effects of granulysin at single-molecule level remains unknown. Here, we produced and purified bactericidal domain of macaque granulysin (GNL). Our bacterial lysis assays suggested that GNL could efficiently kill bacteria such as Listeria monocytogenes. Read More

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December 2012

Twenty-four hour steroid avoidance immunosuppressive regimen in liver transplant recipients.

Exp Clin Transplant 2012 Jun;10(3):258-62

From the Organ Transplant Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Objectives: To investigate the efficacy and safety of an immunosuppressive regimen of steroid avoidance in combination with induction therapy and tacrolimus in liver transplant recipients.

Materials And Methods: Eighty-two adult liver transplant recipients were randomized into 2 groups: standard protocol group (n=41) in which steroids were withdrawn 3 months after the operation, and a 24-hour steroid avoidance group (n=41) in which steroids were eliminated within 24 hours. The incidence of acute rejections, infections (bacterial, fungal, and cytomegalovirus), and metabolic complications were analyzed between the groups. Read More

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Steroid elimination within 24 hours after orthotopic liver transplantation: effectiveness and tolerability.

Hepatobiliary Pancreat Dis Int 2012 Apr;11(2):137-42

Organ Transplantation Center, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.

Background: Steroids have been the mainstay of immunosuppressive regimen in liver transplantation. However, the use of steroids is associated with various post-transplant complications. This study evaluated the efficacy and safety of reduced immunosuppressive regimen with steroids (steroid elimination within 24 hours post-transplant) in a cohort of Chinese liver transplant recipients. Read More

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Sirolimus conversion in liver transplant recipients with calcineurin inhibitor-induced complications: efficacy and safety.

Exp Clin Transplant 2012 Apr;10(2):132-5

Organ Transplant Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Objectives: To evaluate the efficacy and safety of conversion from calcineurin inhibitors to sirolimus among liver transplant recipients with calcineurin inhibitor-induced complications.

Materials And Methods: After receiving liver transplants, 25 patients with calcineurin inhibitor-induced complications (22 renal dysfunction and 3 new-onset diabetes mellitus) were converted from sirolimus to tacrolimus. The serum creatinine, sirolimus trough level, liver function, acute rejection episodes, and drug-related adverse effects were monitored. Read More

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Thermotherapy enhances oxaliplatin-induced cytotoxicity in human colon carcinoma cells.

World J Gastroenterol 2012 Feb;18(7):646-53

Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangdong Province, China.

Aim: To observe the synergistic effects of hyperthermia in oxaliplatin-induced cytotoxicity in human colon adenocarcinoma Lovo cells.

Methods: The human colon adenocarcinoma cell line Lovo was obtained from Sun Yat-Sen University. Cells were sealed with parafilm and placed in a circulating water bath, and was maintained within 0. Read More

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February 2012

[A 17-year study of bloodstream Escherichia coli infection after liver transplantation: resistance rate, risk factor and mortality].

Zhonghua Yi Xue Za Zhi 2011 Nov;91(42):2977-80

Organ Transplantation Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

Objective: To explore the resistance rate, risk factors and mortality of Escherichia coli bloodstream infections (BSI) after liver transplantation.

Methods: From January 1993 to May 2010, a retrospective analysis of Escherichia coli in liver transplants were conducted.

Results: A total of 88 BSI occurred in 83/695 patients and Escherichia coli (n = 23) was most commonly found. Read More

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November 2011

[Experiences of liver retransplantation for postoperative diffuse biliary strictures].

Zhonghua Yi Xue Za Zhi 2011 Jun;91(22):1529-32

Sun Yat-sen University, Guangzhou 510080, China.

Objective: To investigate the feasibility and management of retransplantation for diffuse biliary strictures occurring after initial liver transplantation.

Methods: The clinical data of 53 consecutive liver retransplantation patients at our hospital from January 2001 to December 2009 were collected and analyzed retrospectively. Among them, 20 (37. Read More

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