2,670 results match your criteria hpv genotyping


Genotyping of human papillomavirus DNA in Wielkopolska region.

Ginekol Pol 2021 Sep 20. Epub 2021 Sep 20.

Department of Gynaecological Endocrinology and Gynaecology, Jagiellonian University Medical College, Cracow, Poland.

Objectives: Human papillomavirus infection is one of the most common sexually transmitted diseases. Long-term exposure to the HPV leads to development of high-grade squamous intraepithelial lesions that can eventually transform into cervical cancer. The aim of the study was to assess the HPV genotype distribution in patients with abnormal pap smear and provide prospective study. Read More

View Article and Full-Text PDF
September 2021

Clinical Validation of p16/Ki-67 Dual-Stained Cytology Triage of HPV-Positive Women - Results from the IMPACT Trial.

Int J Cancer 2021 Sep 18. Epub 2021 Sep 18.

Ventana Medical Systems Inc./Roche Tissue Diagnostics, Tucson, AZ.

Triage strategies are needed for primary human papillomavirus (HPV)-based cervical cancer screening to identify women requiring colposcopy/biopsy. We assessed the performance of p16/Ki-67 dual-stained immunocytochemistry (DS) to triage HPV-positive women and compared it to cytology, with or without HPV16/18 genotyping. A prospective observational screening study enrolled 35,263 women aged 25-65 years at 32 U. Read More

View Article and Full-Text PDF
September 2021

Cost-Effectiveness and Budget Impact Analysis of Primary Screening With Human Papillomavirus Test With Genotyping in Argentina.

Value Health Reg Issues 2021 Sep 13;26:160-168. Epub 2021 Sep 13.

Hospital de Clínicas, Buenos Aires, Argentina.

Objectives: Cervical cancer (ICC) is the fourth leading cause of mortality in women in Argentina and primary screening with conventional cytology (Papanicolaou smear) is the most widely used strategy despite its limitations. Strategies based on human papillomavirus (HPV) testing have the potential to improve detection and reduce mortality. The objective of this study is to evaluate the cost-effectiveness and budgetary impact of a strategy based on HPV testing with genotyping. Read More

View Article and Full-Text PDF
September 2021

Prevalence of high-risk human papillomavirus DNA and mRNA and its association with abnormal anal cytology in the Czech male anal cancer screening cohort.

Diagn Cytopathol 2021 Sep 16. Epub 2021 Sep 16.

Biomedical Center of the Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.

Background: Anal cancer (AC) screening is justified in high-risk populations, particularly HIV-positive men having sex with men (MSM). HR-HPV testing could improve the efficiency of cytologically based screening of AC, as in the screening of biologically analogical cervical cancer. The specificity of HR-HPV testing is influenced by the prevalence of HR-HPV infection in the screened population. Read More

View Article and Full-Text PDF
September 2021

Anal human papillomavirus prevalence and risk factors among men who have sex with men in Vietnam.

Int J Infect Dis 2021 Sep 10. Epub 2021 Sep 10.

London School of Hygiene and Tropical Medicine, London, UK; Infection and Immunity, Murdoch Children's Research Institute, VIC, Australia; Department of Paediatrics, The University of Melbourne, VIC, Australia; National Institute for Control of Vaccine and Biologicals, Vietnam.

Objectives: Men who have sex with men (MSM) are at risk of human papillomavirus (HPV)-related cancers, while published data are scarce. We determined HPV prevalence and risk factors in MSM in Vietnam to inform HPV prevention strategies in this key population.

Methods: A cross-sectional study of 799 MSM aged 16 to 50 years old was conducted in Vietnam in 2017-2018. Read More

View Article and Full-Text PDF
September 2021

HPV Genotype Specific and Age Stratified Immediate Prevalence of Cervical Precancers and Cancers in Women with NILM/hrHPV+: A Single Center Retrospective Study of 26,228 Cases.

Cancer Manag Res 2021 2;13:6869-6877. Epub 2021 Sep 2.

Department of Pathology, Zhejiang University School of Medicine Women's Hospital, Hangzhou, 310006, People's Republic of China.

Purpose: To investigate the prevalence of precancers [high-grade squamous intraepithelial lesion (HSIL) and adenocarcinoma in situ (AIS)] and cancers [squamous cell carcinoma (SCC) and adenocarcinoma (ADC)] in various high-risk human papillomavirus (hrHPV) genotypes or age groups among women with negative for intraepithelial lesion or malignancy (NILM) and hrHPV-positive pap results.

Materials And Methods: In total, 26,228 women with NILM/hrHPV+ were included in the study. Among them, 5893 had immediate follow-up biopsy results available and were selected for further prevalence analysis. Read More

View Article and Full-Text PDF
September 2021

Accuracy of combined molecular and morphology-based triage for HPV-positive women in routine cervical cancer screening services from Colombia.

Prev Med 2021 Sep 8:106801. Epub 2021 Sep 8.

Subdirección de Investigación - Instituto Nacional de Cancerología, Bogotá, Colombia. Electronic address:

WHO cervical cancer elimination goals comprise 70% of highly-sensitive screening coverage and 90% treatment of precancerous lesions. Triage for HPV-positive women may challenge sensitivity of screening algorithms and women's follow-up, particularly in low- and middle-income countries (LMIC) where screening quality and protocol adherence are frequently deficient. We aimed to determine the accuracy of triage for HPV positive women in routine screening services from Colombia by a prospective cross-sectional study. Read More

View Article and Full-Text PDF
September 2021

Human DLG1 and SCRIB Are Distinctly Regulated Independently of HPV-16 during the Progression of Oropharyngeal Squamous Cell Carcinomas: A Preliminary Analysis.

Cancers (Basel) 2021 Sep 4;13(17). Epub 2021 Sep 4.

Division of Molecular Medicine, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia.

The major causative agents of head and neck squamous cell carcinomas (HNSCCs) are either environmental factors, such as tobacco and alcohol consumption, or infection with oncogenic human papillomaviruses (HPVs). An important aspect of HPV-induced oncogenesis is the targeting by the E6 oncoprotein of PDZ domain-containing substrates for proteasomal destruction. Tumor suppressors DLG1 and SCRIB are two of the principal PDZ domain-containing E6 targets. Read More

View Article and Full-Text PDF
September 2021

How Much Does HIV Positivity Affect the Presence of Oral HPV? A Molecular Epidemiology Survey.

Int J Environ Res Public Health 2021 Aug 26;18(17). Epub 2021 Aug 26.

Department of Biomedical and Dental Sciences and MorphoFunctional Imaging, University of Messina, 98122 Messina, Italy.

HIV-positive people showed a high oral prevalence of HPV-DNA and have a greater incidence of head and neck carcinomas compared to general population. We performed a molecular survey evaluating the presence of HPV-DNA in saliva of HIV-positive and HIV-negative subjects in order to quantify the risk represented by HIV-positivity. The sample was made up by 102 subjects: 40 HIV-positive, 32 HIV-negative with sexual risk behaviors (SRB) and 30 HIV-negative without risk factors. Read More

View Article and Full-Text PDF

Genital Human Papillomavirus Prevalence and Genotyping Among Males in Putuo District of Shanghai, China 2015-2019.

Med Sci Monit 2021 Sep 3;27:e932093. Epub 2021 Sep 3.

Laboratory Medicine Department, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China (mainland).

BACKGROUND Reports of human papillomavirus (HPV) infection and genotype distribution in Chinese men are limited, and HPV vaccination has not yet been recommended for men in China. MATERIAL AND METHODS We retrospectively reviewed the prevalence and genotyping of male genital HPV. A total of 1227 male patients (aged 17 to 81 years) attending the dermatology and sexually transmitted disease clinics at Putuo District Center Hospital in Shanghai from 2015 to 2019 were included. Read More

View Article and Full-Text PDF
September 2021

Development of models for cervical cancer screening: construction in a cross-sectional population and validation in two screening cohorts in China.

BMC Med 2021 Sep 3;19(1):197. Epub 2021 Sep 3.

Department of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 South Panjiayuan Lane, Beijing, China.

Background: Current methods for cervical cancer screening result in an increased number of referrals and unnecessary diagnostic procedures. This study aimed to develop and evaluate a more accurate model for cervical cancer screening.

Methods: Multiple predictors including age, cytology, high-risk human papillomavirus (hrHPV) DNA/mRNA, E6 oncoprotein, HPV genotyping, and p16/Ki-67 were used for model construction in a cross-sectional population including women with normal cervix (N = 1085), cervical intraepithelial neoplasia (CIN, N = 279), and cervical cancer (N = 551) to predict CIN2+ or CIN3+. Read More

View Article and Full-Text PDF
September 2021

Association of a genetic variant in Interleukin-10 gene with increased risk and inflammation associated with cervical cancer.

Gene 2021 Aug 28;807:145933. Epub 2021 Aug 28.

Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Background: Cervical-cancer is among the most commonly diagnosed cancers in women, and infection with human papillomavirus (HPV) is associated with an increased risk of cervical cancer and altered serum concentrations of inflammatory cytokines. We have explored the association between a genetic variation in the Interleukin-10 (IL-10) gene (rs1800896) and cervical cancer risk and its relationship with tissue Interferon gamma (IFN-γ), Transforming growth factor beta (TGF-β), Tumor necrosis factor alpha (TNF-α) concentrations in women with cervical cancer.

Methods: A total of 315 women with, or without cervical cancer, were recruited into the study. Read More

View Article and Full-Text PDF

Evaluation of DNA extraction protocols from liquid-based cytology specimens for studying cervical microbiota.

PLoS One 2021 30;16(8):e0237556. Epub 2021 Aug 30.

Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America.

Cervical microbiota (CM) are considered an important factor affecting the progression of cervical intraepithelial neoplasia (CIN) and are implicated in the persistence of human papillomavirus (HPV). Collection of liquid-based cytology (LBC) samples is routine for cervical cancer screening and HPV genotyping and can be used for long-term cytological biobanking. We sought to determine whether it is possible to access microbial DNA from LBC specimens, and compared the performance of four different extraction protocols: (ZymoBIOMICS DNA Miniprep Kit; QIAamp PowerFecal Pro DNA Kit; QIAamp DNA Mini Kit; and IndiSpin Pathogen Kit) and their ability to capture the diversity of CM from LBC specimens. Read More

View Article and Full-Text PDF

Clinical implications of alpha, beta, and gamma HPV infection in juvenile onset recurrent respiratory papillomatosis.

Eur Arch Otorhinolaryngol 2021 Aug 28. Epub 2021 Aug 28.

Department of Neurosciences, Section of Otolaryngology, University of Padova, Padova, Italy.

Purpose: The aim of our study was to evaluate the prevalence of different HPV genera-alpha, beta and gamma-in Juvenile onset Recurrent Respiratory Papillomatosis (JoRRP) and examine the association of type and genus-specific viral features with the clinical outcome of disease.

Methods: This retrospective observational study included consecutive patients with JoRRP who were treated in a referral centre between October 2000 and October 2020. All patients underwent cold excision and laser vaporisation of papillomatous lesions. Read More

View Article and Full-Text PDF

HPV DeepSeq: An Ultra-Fast Method of NGS Data Analysis and Visualization Using Automated Workflows and a Customized Papillomavirus Database in CLC Genomics Workbench.

Pathogens 2021 Aug 13;10(8). Epub 2021 Aug 13.

Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA.

Next-generation sequencing (NGS) has actualized the human papillomavirus (HPV) virome profiling for in-depth investigation of viral evolution and pathogenesis. However, viral computational analysis remains a bottleneck due to semantic discrepancies between computational tools and curated reference genomes. To address this, we developed and tested automated workflows for HPV taxonomic profiling and visualization using a customized papillomavirus database in the CLC Microbial Genomics Module. Read More

View Article and Full-Text PDF

Stratified Mucin-Producing Intraepithelial Lesion (SMILE) of the Uterine Cervix: High-Risk HPV Genotype Predominance and p40 Immunophenotype.

Cells 2021 Aug 10;10(8). Epub 2021 Aug 10.

Institute of Pathology, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia.

Stratified mucin-producing intraepithelial lesion (SMILE) is a rare high-grade cervical precancerous lesion designated a variant of adenocarcinoma in situ (AIS) in the WHO classification. We aimed to determine HPV genotypes, immunohistochemical phenotype and mucin presence in SMILE. Between 2010 and 2018, SMILE was diagnosed in 34 out of 6958 (0. Read More

View Article and Full-Text PDF

The Distribution and Prevalence of High-Risk HPV Genotypes Other than HPV-16 and HPV-18 among Women Attending Gynecologists' Offices in Kazakhstan.

Biology (Basel) 2021 Aug 18;10(8). Epub 2021 Aug 18.

Department of Biomedical Sciences, School of Medicine, Nazarbayev University, Nur-Sultan 010000, Kazakhstan.

Cervical cancer represents a great burden to public health of women. This study aimed to obtain a nationwide genotyping survey and analysis of high risk-HPV including those that are caused by HPV types other than HPV-16 and HPV-18, among women in Kazakhstan. This study was conducted based on the collection of survey and cervical swabs of 1645 women across the country. Read More

View Article and Full-Text PDF

HPV-specific risk assessment of cervical cytological abnormalities.

BMC Cancer 2021 Aug 24;21(1):949. Epub 2021 Aug 24.

Department of Gynecological Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang West Road, Guangzhou, People's Republic of China.

Background: Cytology and HPV genotype screening play an important role in cervical cancer detection. Whether multiple HPV genotyping can predict cytological lesions remains to be further studied.

Methods: Two thousand two hundred twenty-four females were analyzed for cytology and HPV genotypes test. Read More

View Article and Full-Text PDF

Recurrent respiratory papillomatosis: clinical characteristics and viral genotyping in a Brazilian population.

Rev Inst Med Trop Sao Paulo 2021 16;63:e63. Epub 2021 Aug 16.

Universidade Federal do Maranhão, Pós-Graduação em Saúde do Adulto, São Luís, Maranhão, Brazil.

This study presents 25 cases of recurrent respiratory papillomatosis (RRP) that occurred in Sao Luis, Maranhao State, Northeast region, Brazil, between January 2007 and December 2018. Sociodemographic and clinical profile of patients as well as human papillomavirus (HPV) infection status were evaluated. Clinical and histopathological data were collected from the patients' medical records. Read More

View Article and Full-Text PDF

Comparative pilot study about HPV test with partial genotyping in primary screening versus other strategies for cervical cancer population screening, CRYGEN 16/18 study.

Enferm Infecc Microbiol Clin 2021 Aug 14. Epub 2021 Aug 14.

Servicio de Microbiología, Hospital Universitario Ramón y Cajal. IRYCIS, Madrid, España; CIBER Epidemiología Salud Pública (CIBERESP), Madrid, España. Electronic address:

Introduction: The early detection of cervical cancer requires the implementation of molecular screening programs for human papillomavirus (HPV). However, there are discrepancies in the optimization of screening protocols. The performance of 10 primary screening strategies based on molecular, cytological or combined techniques is now evaluated. Read More

View Article and Full-Text PDF

Nested PCR followed by NGS: Validation and application for HPV genotyping of Tunisian cervical samples.

PLoS One 2021 11;16(8):e0255914. Epub 2021 Aug 11.

Department of Molecular Epidemiology and Experimental Pathology, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis, Tunisia.

The most used methodologies for HPV genotyping in Tunisian studies are based on hybridization that are limited to a restricted number of HPV types and to a lack of specificity and sensitivity for same types. Recently, Next-Generation sequencing (NGS) technology has been efficiently used for HPV genotyping. In this work we designed and validated a sensitive genotyping method based on nested PCR followed by NGS. Read More

View Article and Full-Text PDF

Multiple Human Papilloma Virus (HPV) Infections Are Associated with HSIL and Persistent HPV Infection Status in Korean Patients.

Viruses 2021 07 12;13(7). Epub 2021 Jul 12.

Department of Pathology, Kyungpook National University Chilgok Hospital, Kyungpook National University School of Medicine, Daegu 41566, Korea.

Infections with multiple human papilloma virus (HPV) types have been reported, but their role in cervical carcinogenesis has not been fully elucidated. In this study, 236 cases with multiple HPV infection were examined and compared to 180 cases with single HPV infection. HPV genotyping was performed with cervico-vaginal swab specimens using multiplex (real-time) polymerase chain reaction (PCR). Read More

View Article and Full-Text PDF

Human Papillomavirus Same Genotype Persistence and Risk of Cervical Intraepithelial Neoplasia2+ Recurrence.

Cancers (Basel) 2021 Jul 21;13(15). Epub 2021 Jul 21.

Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy.

To evaluate the significance of HPV persistence as a predictor for the development of CIN2+ recurrence and the impact of multiple genotypes and of HPV 16/18 on recurrence risk. A prospective cohort observational study was carried out at the European Institute of Oncology, Milan, Italy, from December 2006 to December 2014. A total of 408 women surgically treated by excisional procedure for pre-neoplastic and neoplastic cervical lesions were enrolled. Read More

View Article and Full-Text PDF

Prevalence of Human Papillomavirus and Genotype Distribution in Pregnant and Non-Pregnant Women in China.

Risk Manag Healthc Policy 2021 28;14:3147-3157. Epub 2021 Jul 28.

Department of Gynecology, Laboratory of Gynecologic Oncology, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, People's Republic of China.

Purpose: The status of human papillomavirus (HPV) infection in pregnant and non-pregnant women in China remains unclear. This study aimed to compare the prevalence and genotype distributions of HPV between pregnant and non-pregnant women in China.

Patients And Methods: A case-control study was conducted of pregnant women during the second trimester and age-matched non-pregnant women attending the Fujian Maternity and Child Health Hospital between January 1, 2017 and December 31, 2018. Read More

View Article and Full-Text PDF

Performance of cervical cancer screening and triage strategies among women living with HIV in China.

Cancer Med 2021 Sep 2;10(17):6078-6088. Epub 2021 Aug 2.

Department of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objectives: To evaluate the clinical performance of liquid-based cytology (LBC), HPV tests and visual inspections with acetic acid or Lugol's iodine (VIA/VILI) as primary screening and triage strategies among Chinese women living with HIV (WLHIV).

Methods: WLHIV aged 18 years and older were recruited from HIV/AIDS treatment clinic in Yunnan, China from 2019 to 2020. Women were screened with self- and physician-sampling for HPV tests, LBC, and VIA/VILI. Read More

View Article and Full-Text PDF
September 2021

Epidemiology of anal human papillomavirus infection and high-grade squamous intraepithelial lesions in 29 900 men according to HIV status, sexuality, and age: a collaborative pooled analysis of 64 studies.

Lancet HIV 2021 09 30;8(9):e531-e543. Epub 2021 Jul 30.

Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Background: Robust age-specific estimates of anal human papillomavirus (HPV) and high-grade squamous intraepithelial lesions (HSIL) in men can inform anal cancer prevention efforts. We aimed to evaluate the age-specific prevalence of anal HPV, HSIL, and their combination, in men, stratified by HIV status and sexuality.

Methods: We did a systematic review for studies on anal HPV infection in men and a pooled analysis of individual-level data from eligible studies across four groups: HIV-positive men who have sex with men (MSM), HIV-negative MSM, HIV-positive men who have sex with women (MSW), and HIV-negative MSW. Read More

View Article and Full-Text PDF
September 2021

Influence of HIV infection on the distribution of high-risk HPV types among women with cervical precancerous lesions in Yaounde, Cameroon.

Int J Infect Dis 2021 Jul 29;110:426-432. Epub 2021 Jul 29.

Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.

Objectives: To characterize high-risk HPV types associated with cervical precancerous lesions in women living in Yaounde, Cameroon, and to determine their distribution with HIV status.

Methods: Women with abnormal pap smears recorded from February 2015 to May 2019 at Saint Martin de Porres' Health Centre, Yaounde, Cameroon, were recruited in this study after obtaining informed consent. Pap smears were collected and re-examined. Read More

View Article and Full-Text PDF

Effectiveness Of Human Papillomavirus (HPV) Vaccination Against Penile Hpv Infection In Men Who Have Sex With Men And Transgender Women.

J Infect Dis 2021 Jul 28. Epub 2021 Jul 28.

Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Background: In the United States, HPV vaccination has been recommended since 2011 for males aged 11-12 years, with catch-up vaccination recommended through age 26 years for previously unvaccinated men who have sex with men (MSM).

Methods: During 2016-2018, a cross-sectional study enrolled MSM and transgender women aged 18-26 years in Seattle, Washington. Participants submitted self-collected penile swab specimens for HPV genotyping. Read More

View Article and Full-Text PDF

The Potential Impact of High-Risk Human Papillomavirus-Negative Cervical Intraepithelial Neoplasia 2+ on Primary Human Papillomavirus Screening.

Am J Clin Pathol 2021 Jul 24. Epub 2021 Jul 24.

Preventive Gynecology Unit, European Institute of Oncology IRCCS, Milan, Italy.

Objectives: To investigate the prevalence of high-risk human papillomavirus (HPV)-negative cervical intraepithelial neoplasia (CIN) and invasive cervical carcinoma (ICC) and to analyze the distribution of other genotypes in this subset.

Methods: In total, 431 women who underwent excisional surgical treatment for CIN or ICC at the European Institute of Oncology, Milan, Italy, from January 2016 to December 2017 were retrospectively analyzed. The Linear Array HPV genotyping test (Roche Diagnostics) was performed on a postaliquot from high-risk-HPV-negative liquid-based cervical specimens, when available. Read More

View Article and Full-Text PDF

Prevalence of High-Risk Human Papillomavirus Infections before and after Cervical Lesion Treatment, among Women Living with HIV.

J Clin Med 2021 Jul 15;10(14). Epub 2021 Jul 15.

Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Even when receiving combination antiretroviral therapy, women living with HIV are at high risk of human papillomavirus (HPV) infection and/or cervical lesions, including cancer. Using data from the PapilloV prospective cohort, we evaluated the prevalence of high-risk HPV (HR-HPV) infections after cervical lesion treatment and investigated factors associated with their carriage. Women were followed up for three years with annual Pap smear and HPV genotyping. Read More

View Article and Full-Text PDF