17,449 results match your criteria hpv -dna

Prevalence and Genotype Distribution of Human Papillomavirus Infection among 12,076 Iranian Women.

Int J Infect Dis 2021 Jul 31. Epub 2021 Jul 31.

Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Teheran, Iran.. Electronic address:

Introduction: Human papillomavirus (HPV) infection is one of the major health concerns of women in developing countries. This study gives an insight into the prevalence and genotype distribution of HPV infection and compares it with Pap smear results among Iranian women.

Methods: In this study, 12,076 Iranian women underwent routine examination from November 2016 to November 2018 using HPV Direct Flow CHIP System for HPV DNA typing. Read More

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HPV-16 E6/E7 DNA tattoo vaccination using genetically optimized vaccines elicit clinical and immunological responses in patients with usual vulvar intraepithelial neoplasia (uVIN): a phase I/II clinical trial.

J Immunother Cancer 2021 Aug;9(8)

Center for Gynecologic Oncology Amsterdam (CGOA), The Netherlands Cancer Institute - Antoni van Leeuwenhoek, Amsterdam, The Netherlands

Background: Usual vulvar intraepithelial neoplasia (uVIN) is a premalignancy caused by persistent infection with high-risk types of human papillomavirus (HPV), mainly type 16. Even though different treatment modalities are available (eg, surgical excision, laser evaporation or topical application of imiquimod), these treatments can be mutilating, patients often have recurrences and 2%-8% of patients develop vulvar carcinoma. Therefore, immunotherapeutic strategies targeting the pivotal oncogenic HPV proteins E6 and E7 are being explored to repress carcinogenesis. Read More

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Nodal tumor volume as a prognostic factor for head and neck squamous cell carcinoma: a systematic review.

Front Biosci (Landmark Ed) 2021 Jul;26(7):235-245

1st Department of Otolaryngology - Head & Neck Surgery, Medical School, National & Kapodistrian University of Athens (NKUA), Hippokrateio Hospital, 11527 Athens, Greece.

: Several studies suggest that there is an association between the metastatic nodal tumor volume and the clinical outcome in patients with solid cancers. However, despite the prognostic potential of nodal volume, a standardized method for estimating the nodal volumetric parameters is lacking. Herein, we conducted a systematic review of the published scientific literature towards investigating the prognostic value of nodal volume in the carcinomas of head and neck, taking into consideration the primary tumor site and the human papillomavirus (HPV) status. Read More

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Current salivary biomarkers for detection of human papilloma virus-induced oropharyngeal squamous cell carcinoma.

Head Neck 2021 Jul 31. Epub 2021 Jul 31.

Department of Otolaryngology, University of Miami Miller School of Medicine, Miami, Florida, USA.

Human papilloma virus (HPV) infection is a key risk factor and etiology for oropharyngeal squamous cell carcinoma (OPSCC). HPV-induced OPSCC is rapidly increasing in incidence, with men experiencing increased mortality. When identified at an early stage, HPV-induced OPSCC can be successfully treated. Read More

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Salivary High-Risk Human Papillomavirus DNA as a Biomarker for Human Papillomavirus-Driven Head and Neck Cancers.

J Mol Diagn 2021 Jul 26. Epub 2021 Jul 26.

Saliva & Liquid Biopsy Translational Laboratory, School of Biomedical Science, Faculty of Health, Queensland University of Technology (QUT), Queensland, Australia; Translational Research Institute, Queensland, Australia. Electronic address:

High-risk human papillomavirus (HR-HPV) infection is one of the major risk factors for head and neck cancers (HNC). Despite the rising incidence, biomarkers for detection, prognostication and disease monitoring of HPV-driven HNC (HPV-HNC) are lacking. To evaluate the capacity of salivary HR-HPV DNA as a biomarker for HPV-HNC, salivary HR-HPV status of 491 primary HNC patients and 10 recurrent HNC patients was investigated at diagnosis using qPCR and MassARRAY. Read More

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The genetic diversity of "papillomavirome" in bovine teat papilloma lesions.

Anim Microbiome 2021 Jul 28;3(1):51. Epub 2021 Jul 28.

Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS, Brazil.

Background: Papillomaviruses are small nonenveloped, circular double-stranded DNA viruses that belong to the Papillomaviridae family. To date, 29 Bos taurus papillomavirus (BPV) types have been described. Studies involving mixed BPV infections have rarely been reported in contrast to human papillomavirus (HPV), which is commonly described in numerous studies showing coinfections. Read More

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Eliminating Cervical Cancer: Progress and Challenges for High-income Countries.

Clin Oncol (R Coll Radiol) 2021 Jul 24. Epub 2021 Jul 24.

Gynaecological Oncology Research Group, Division of Cancer Sciences, University of Manchester, Faculty of Biology, Medicine and Health, Manchester, UK; Department of Obstetrics and Gynaecology, St Mary's Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.

In 2020, the World Health Organization launched a major initiative to eliminate cervical cancer globally. The initiative is built around the three key pillars of human papillomavirus (HPV) vaccination, cervical screening and treatment, with associated intervention targets for the year 2030. The '90-70-90' targets specify that 90% of adolescent girls receive prophylactic HPV vaccination, 70% of adult women receive a minimum twice-in-a-lifetime cervical HPV test and 90% receive appropriate treatment for preinvasive or invasive disease. Read More

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Triple signal amplification strategy for the ultrasensitive electrochemical detection of human papillomavirus 16 E6/E7 mRNA.

Enzyme Microb Technol 2021 Sep 19;149:109855. Epub 2021 Jun 19.

State Key Laboratory of Pharmaceutical Biotechnology, Department of Rheumatology and Immunology, The Affiliated Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, School of Life Sciences, Nanjing University, Nanjing, 210023, China. Electronic address:

Human papilloma virus (HPV) is the primary causative agent of cervical, vaginal, and vulvar cancers. HPV E6/E7 mRNA detection has been proven to improve the specificity and positive predictive value compared with HPV DNA testing in screening, whereby, it may possess higher diagnostic potential. Herein, to establish the ultrasensitive and specific detection of HPV E6/E7 mRNA, we developed a novel triple signal amplification strategy, combined with gold nanoparticles (AuNPs), reverse transcription loop-mediated isothermal amplification (RT-LAMP) and high affinity biotin-avidin system. Read More

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September 2021

Characterisation of anal intraepithelial neoplasia and anal cancer in HIV-positive men by immunohistochemical markers p16, Ki-67, HPV-E4, and DNA methylation markers.

Int J Cancer 2021 Jul 26. Epub 2021 Jul 26.

Department of Pathology, Cancer Center Amsterdam CCA, Amsterdam University Medical Centers (Amsterdam UMC), Vrije Universiteit Amsterdam, De Boelelaan 1117, Amsterdam, The Netherlands.

Human papillomavirus (HPV)-induced anal intraepithelial neoplasia (AIN, graded 1-3) is highly prevalent in HIV-positive (HIV+) men-who-have-sex-with-men (MSM), but only a minority of lesions progresses to cancer. This study aimed to characterise comprehensively anal tissue samples from a cross-sectional series (n = 104) of HIV + MSM and longitudinal series (n = 40) of AIN2/3 progressing to cancer using different biomarkers. The cross-sectional series consisted of 8 normal, 26 AIN1, 45 AIN2, 15 AIN3 and 10 anal squamous cell carcinoma. Read More

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In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses.

Mol Biotechnol 2021 Jul 25. Epub 2021 Jul 25.

Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Human papillomaviruses (HPV)-16 and 18 are the most prevalent types associated with cervical cancer. HPV L1 and L2 capsid proteins and E7 oncoprotein play crucial roles in HPV-related diseases. Hence, these proteins were proposed as target antigens for preventive and therapeutic vaccines. Read More

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Detection of HPV E6/E7 mRNA in the diagnosis of cervical cancer and precancerous lesions after kidney transplantation.

Am J Transl Res 2021 15;13(6):7312-7317. Epub 2021 Jun 15.

Department of Gynecology and Obstetrics, Yinzhou No. 2 Hospital of Ningbo Ningbo, Zhejiang Province, China.

Objective: To explore the value of human papillomavirus (HPV) E6/E7 mRNA detection in the diagnosis of cervical cancer and its precancerous lesions after kidney transplantation.

Methods: One hundred and sixty-six women who underwent cervical cancer screening after kidney transplantation were selected and received thinprep cytology test (TCT), HPV DNA and HPV E6/E7 mRNA tests. A biopsy under colposcopy was performed for those with suspicious test results. Read More

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Human papillomavirus in retinoblastoma: A tertiary eye care center study from South India.

Indian J Ophthalmol 2021 08;69(8):2111-2115

Department of Molecular Genetics, Aravind Medical Research Foundation, Madurai; Department of Molecular Biology, Aravind Medical Research Foundation, Affiliated to Alagappa University, Karaikudi, India.

Purpose: This study is aimed to investigate the presence of Human papillomavirus (HPV) DNA in tumors obtained from sporadic retinoblastoma patients.

Methods: One hundred six tumor tissues obtained from sporadic RB patients were analyzed for HPV infection by use of both seminested PCR and real-time quantitative PCR.

Results: Of 106 RB patients, 55 were male and 51 were female. Read More

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The characteristics and risk factors of human papillomavirus infection: an outpatient population-based study in Changsha, Hunan.

Sci Rep 2021 Jul 23;11(1):15128. Epub 2021 Jul 23.

Department of Obstetrics and Gynecology, The Third Xiangya Hospital of Central South University, Changsha, 410013, China.

This cross-sectional study investigated the characteristics of cervical HPV infection in Changsha area and explored the influence of Candida vaginitis on this infection. From 11 August 2017 to 11 September 2018, 12,628 outpatient participants ranged from 19 to 84 years old were enrolled and analyzed. HPV DNA was amplified and tested by HPV GenoArray Test Kit. Read More

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Liquid Biopsy Goes Viral: Next-Generation Sequencing to Enhance HPV Detection.

Clin Cancer Res 2021 Jul 22. Epub 2021 Jul 22.

Radiation Medicine Program, Princess Margaret Cancer Centre

Liquid biopsy approaches for the detection of viral DNA can provide important information for the diagnosis and identification of virally-associated cancers. Here we discuss the next-generation sequencing (NGS)-based CaptHPV method for the detection and characterization of plasma human papillomavirus (HPV) DNA in HPV-associated cancers and its potential clinical utility. Read More

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HPV Infection Leaves a DNA Methylation Signature in Oropharyngeal Cancer Affecting Both Coding Genes and Transposable Elements.

Cancers (Basel) 2021 Jul 20;13(14). Epub 2021 Jul 20.

Molecular Carcinogenesis Program, Brazilian National Cancer Institute, Rio de Janeiro CEP 20231-050, Brazil.

HPV oncoproteins can modulate DNMT1 expression and activity, and previous studies have reported both gene-specific and global DNA methylation alterations according to HPV status in head and neck cancer. However, validation of these findings and a more detailed analysis of the transposable elements (TEs) are still missing. Here we performed pyrosequencing to evaluate a 5-CpG methylation signature and Line1 methylation in an oropharyngeal squamous cell carcinoma (OPSCC) cohort. Read More

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Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression.

Cancers (Basel) 2021 Jul 14;13(14). Epub 2021 Jul 14.

Oncovirology Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro 20231-050, Brazil.

Retroelements are expressed in diverse types of cancer and are related to tumorigenesis and to cancer progression. We characterized the expression of retroelements in cervical cancer and explored their interplay with HPV infection and their association with expression of neighboring genes. Forty biopsies of invasive cervical carcinoma (squamous cell carcinomas and adenocarcinomas) with genotyped HPV were selected and analyzed for human endogenous retrovirus (HERV) and long interspersed nuclear element 1 (L1) expression through RNA-seq data. Read More

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Signal Extraction, Transformation, and Magnification for Ultrasensitive and Specific Detection of Nucleic Acids.

Anal Chem 2021 Aug 23;93(30):10611-10618. Epub 2021 Jul 23.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, China.

Nucleic acid noises caused by the background and nonspecificity amplifications can jeopardize accurate polymerization and detection of nucleic acids, especially when they are analyzed in low copies. We hypothesize to reduce the noises by designing a system for specific signal extraction, transformation, and magnification to improve the specificity and sensitivity. Herein, by developing an extractor-trigger complex (ET-Combo) for the system, we have established isothermal and hybridizing combined amplifications: a one-pot detection system with two-step amplification coupled by ET-Combo. Read More

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The Association of Human Papillomavirus in Benign and Malignant Laryngeal Lesions-a Pilot Study.

Indian J Surg Oncol 2021 Jun 13;12(2):306-310. Epub 2020 Jun 13.

Department of Head and Neck surgery, Christian Medical College Hospital, Vellore, 632004 India.

Various etiological factors have been described in laryngeal carcinogenesis. Tobacco and alcohol play a major role in the development of laryngeal cancers. However, recently there has been an overshadowing of association of human papillomavirus infection in laryngeal cancers. Read More

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Performance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women.

PLoS One 2021 21;16(7):e0254946. Epub 2021 Jul 21.

Facultad de Medicina, Centro de Investigación en Políticas, Población y Salud, Universidad Nacional Autónoma de México, Mexico City, Mexico.

Introduction: Urine self-sampling for human papillomavirus (HPV)-based cervical cancer screening is a non-invasive method that offers several logistical advantages and high acceptability, reducing barriers related to low screening coverage. This study developed and evaluated the performance of a low-cost urine self-sampling method for HPV-testing and explored the acceptability and feasibility of potential implementation of this alternative in routine screening.

Methods: A series of sequential laboratory assays examined the impact of several pre-analytical conditions for obtaining DNA from urine and subsequent HPV detection. Read More

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The Biological Basis for Enhanced Effects of Proton Radiation Therapy Relative to Photon Radiation Therapy for Head and Neck Squamous Cell Carcinoma.

Int J Part Ther 2021 25;8(1):3-13. Epub 2021 Jun 25.

Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Head and neck squamous cell carcinomas (HNSCCs) often present as local-regionally advanced disease at diagnosis, for which a current standard of care is x-ray-based radiation therapy, with or without chemotherapy. This approach provides effective local regional tumor control, but at the cost of acute and late toxicity that can worsen quality of life and contribute to mortality. For patients with human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (SCC) in particular, for whom the prognosis is generally favorable, de-escalation of the radiation dose to surrounding normal tissues without diminishing the radiation dose to tumors is desired to mitigate radiation-related toxic effects. Read More

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Serum HPV16 E7 Oncoprotein Is a Recurrence Marker of Oropharyngeal Squamous Cell Carcinomas.

Cancers (Basel) 2021 Jul 5;13(13). Epub 2021 Jul 5.

Department of Medical Sciences, University of Ferrara, 44121 Ferrara, Italy.

Despite improved prognosis for many HPV-positive head and neck squamous cell carcinomas (HNSCCs), some cases are still marked by recurrence and metastasis. Our study aimed to identify novel biomarkers for patient stratification. Classical HPV markers: HPV-DNA, p16 and HPV mRNA expression were studied in HNSCC ( = 67) and controls ( = 58) by qPCR. Read More

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Evaluation of Integrated HPV DNA as Individualized Biomarkers for the Detection of Recurrent CIN2/3 during Post-Treatment Surveillance.

Cancers (Basel) 2021 Jul 1;13(13). Epub 2021 Jul 1.

Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe, Technische Universität Dresden, 01307 Dresden, Germany.

Purpose: Post-treatment follow-up in women with cervical pre-cancers (CIN3) is mandatory due to relapse in up to 10% of patients. Standard follow-up based on hrHPV-DNA/cytology co-testing has high sensitivity but limited specificity. The aim of our prospective, multicenter, observational study was to test the hypothesis that an individualized viral-cellular-junction test (vcj-PCR) combined with cytology has a lower false positive rate for the prediction of recurrence compared to standard co-testing. Read More

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Is There a Role for Sex Hormone Receptors in Head-and-neck Cancer? Links with HPV Infection and Prognosis.

Anticancer Res 2021 Aug;41(8):3707-3716

BTMA, Post-Graduate Programme in Adult Health (PPGSAD), Federal University of Maranhão, São Luis, Brazil.

Background/aim: Head-and-neck squamous cell carcinoma (HNSCC) is the fifth most common cancer in the world and human papillomavirus (HPV) is an important risk factor for this neoplasm. Recent studies showed an association between sex hormone receptors and pathogenesis and/or prognosis in patients with HNSCC. The aim of this study was to clarify the expression patterns of sex hormone receptors in HPV-positive and HPV-negative HNSCC and their associations with tumour biopathology and biological behaviour. Read More

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A Novel Model for Papillomavirus-Mediated Anal Disease and Cancer Using the Mouse Papillomavirus.

mBio 2021 Jul 20:e0161121. Epub 2021 Jul 20.

McArdle Laboratory for Cancer Research, University of Wisconsin School of Medicine and Public Healthgrid.471391.9, Madison, Wisconsin, USA.

Up to 95% of all anal cancers are associated with infection by human papillomavirus (HPV); however, no established preclinical model exists for high-grade anal disease and cancer mediated by a natural papillomavirus infection. To establish an infection-mediated model, we infected both immunocompromised NSG and immunocompetent FVB/NJ mice with the recently discovered murine papillomavirus MmuPV1, with and without the additional cofactors of UV B radiation (UVB) and/or the chemical carcinogen 7,12-dimethylbenz(a)anthracene (DMBA). Infections were tracked via lavages and swabs for MmuPV1 DNA, and pathology was assessed at the endpoint. Read More

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CRISPR/Cas9-based inactivation of human papillomavirus oncogenes E6 or E7 induces senescence in cervical cancer cells.

Virology 2021 Jul 14;562:92-102. Epub 2021 Jul 14.

Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, SE-75123, Uppsala, Sweden. Electronic address:

Human papillomaviruses (HPVs) such as HPV16 and HPV18 can cause cancers of the cervix, anogenital and oropharyngeal sites. Continuous expression of the HPV oncoproteins E6 and E7 are essential for transformation and maintenance of cancer cells. Therefore, therapeutic targeting of E6 or E7 genes can potentially treat HPV-related cancers. Read More

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Electric field-enhanced electrochemical CRISPR biosensor for DNA detection.

Biosens Bioelectron 2021 Jul 12;192:113498. Epub 2021 Jul 12.

Department of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030, United States. Electronic address:

DNA detection plays an important role in the rapid screening of cancers and early diagnosis of infectious diseases. Here, we developed a simple, versatile, electric field-enhanced (EFE), electrochemical CRISPR biosensor to detect DNA targets in a homogeneous solution phase. To improve the detection sensitivity, we applied a pulsed electric field to enrich nucleic acids on the electrode surface. Read More

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The clinical significance and utility of HPV-DNA testing in Korean women with atypical glandular cells in cervical Pap tests: An analysis of 311 cases at a single institution.

Cancer Invest 2021 Jul 19:1-15. Epub 2021 Jul 19.

Department of Obstetrics and Gynecology, Asan Medical center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea.

The aim of this study is to analyze the correlation between clinically significant histologic results and HPV in women with AGC in pap test. Of the 311 women confirmed as AGC, 111 women (35.7%) was identified as positive for HPV. Read More

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Human papillomavirus infection and tumor microenvironment are associated with the microbiota in patients with oropharyngeal cancers-pilot study.

Head Neck 2021 Jul 19. Epub 2021 Jul 19.

Faculty of Medicine, The University of Queensland, Brisbane, Queensland, Australia.

Background: Previous microbiome studies of oropharyngeal cancer have shown that there are differences in the oral microbiota between human papillomavirus (HPV)-positive and HPV-negative patients.

Methods: We collected saliva, normal tissue, and tumor biopsies from 13 patients with oropharyngeal cancer (eight HPV-positive, five HPV-negative). We obtained basic clinical data from each patient. Read More

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Human papillomavirus (HPV) types 16 and 18 infection and esophageal squamous cell carcinoma: a systematic review and meta-analysis.

J Cancer Res Clin Oncol 2021 Jul 17. Epub 2021 Jul 17.

General Surgery 2, ASST Bergamo Ovest, Treviglio, BG, Italy.

Objective: The human papillomavirus (HPV) is implicated in the pathogenesis of several cancers among humans. The role of HPV as one of the etiological agents in esophageal carcinogenesis is partially unknown. We assessed whether the available evidence supports the association of HPV with risk and prognosis in patients with esophageal squamous cell carcinomas (ESCCs). Read More

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Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women.

Infect Agent Cancer 2021 Jul 16;16(1):52. Epub 2021 Jul 16.

Department of Human and Experimental Pathology, Pasteur Institute of Tunis, University Tunis El Manar, Tunis, Tunisia.

Background: High-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC) which represents the second most prevalent gynecological cancer among Tunisian women. Preventive strategies against CC are based on prophylactic vaccines that have not yet been implemented into the national vaccination program of Tunisia. Therefore, the present study aimed to investigate the HPV genotypes distribution in cervical neoplasia in Tunisian women in order to predict the impact of using current HPV vaccines on cancer prevention in Tunisia. Read More

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