Biosens Bioelectron 2021 Apr 12;184:113239. Epub 2021 Apr 12.
Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, PR China; Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000, PR China; Translational Medicine Research Center, North Sichuan Medical College, Nanchong, 637000, PR China. Electronic address:
It is highly desired to perform accurate and rapid nucleic acid detections for disease diagnosis at resource-limited setting, such as small clinics, remote areas and home. However, the challenges in sample handling, expensive equipment and complicated operation make canonical polymerase chain reaction (PCR) impossible to run the point-of-care testing (POCT). Herein we report a novel nucleic acid detection method, named stem-loop-primer assisted isothermal amplification (SPA), which specifically and sensitively amplifies target nucleic acid by using Bst DNA polymerase, a pair of canonical PCR primers and their stem-loop derivatives. Read More