64 results match your criteria hole streptococcus

A Case Report of Bacterial Meningitis Caused by an Emerging Strain of Penicillin-Resistant Non-vaccine Serotype 10A.

Jpn J Infect Dis 2021 Jan 29. Epub 2021 Jan 29.

Office of Infection Control, National Center for Child Health and Development, Japan.

The pneumococcal conjugate vaccines successfully decreased the incidence of invasive pneumococcal diseases and pneumococcal antibiotic resistance. However, it has also led to serotype replacement. According to the report from the National Institute of Infectious Diseases (NIID) in 2017, 96% of pneumococcal isolates obtained from IPD children aged <5 years were non-PCV13-serotype. Read More

View Article and Full-Text PDF
January 2021

Towards improved outcome in children treated surgically for spontaneous intracranial suppuration in South Wales.

Br J Neurosurg 2021 Jan 11:1-4. Epub 2021 Jan 11.

Department of Paediatric Neurosurgery, University Hospital of Wales & Noah's Ark Children's Hospital, Cardiff, UK.

Introduction: Spontaneous central nervous system (CNS) infections in children are rare. Treatment involves surgical intervention and antibiotic therapy. We describe a single centre experience of managing this condition in South Wales. Read More

View Article and Full-Text PDF
January 2021

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments.

Ahmad Almehmadi

J Oral Implantol 2020 Aug 11. Epub 2020 Aug 11.

King Abdulaziz University Faculty of Dentistry Assistant Professor Oral Biology Jamea St SAUDI ARABIA Jeddah West 21463 King Abdulaziz University Faculty of Dentistry.

The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Read More

View Article and Full-Text PDF

In vitro anticariogenic and antibiofilm activities of toothpastes formulated with essential oils.

Arch Oral Biol 2020 Sep 9;117:104834. Epub 2020 Jul 9.

Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address:

Objective: This study aimed to assess the antibacterial and antibiofilm effects of essential oils and herbal toothpastes against bacteria associated with oral diseases.

Methods: The minimum inhibitory concentration (MIC) and antibiofilm activity of 13 essential oils against Staphylococcus aureus, Streptococcus mutans, Lactobacillus lactis, and Enterococcus faecalis. were determined. Read More

View Article and Full-Text PDF
September 2020

Microbial Communities in Human Milk Relate to Measures of Maternal Weight.

Front Microbiol 2019 20;10:2886. Epub 2019 Dec 20.

Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, NH, United States.

The process of breastfeeding exposes infants to bioactive substances including a diversity of bacteria from breast milk as well as maternal skin. Knowledge of the character of and variation in these microbial communities, as well as the factors that influence them, is limited. We aimed to identify profiles of breastfeeding-associated microbial communities and their association with maternal and infant factors. Read More

View Article and Full-Text PDF
December 2019

Enhanced Antibacterial Property of Facet-Engineered TiO Nanosheet in Presence and Absence of Ultraviolet Irradiation.

Materials (Basel) 2019 Dec 22;13(1). Epub 2019 Dec 22.

Joining and Welding Research Institute, Osaka University, 11-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

Titania (TiO) has attracted much attention recently for reducing bacterial diseases by the generation of reactive oxygen species (ROS) under UV irradiation. However, demand for higher photocatalytic activity due to higher recombination of electron and hole remains. The aims of this study were to make titania with higher antibacterial property and show the mechanisms of the bactericidal effect. Read More

View Article and Full-Text PDF
December 2019

Multiscale Computational Study on the Catalytic Mechanism of the Nonmetallo Amidase Maleamate Amidohydrolase (NicF).

J Phys Chem A 2019 Sep 3;123(36):7710-7719. Epub 2019 Sep 3.

Department of Chemistry and Biochemistry , University of Windsor , Windsor , Ontario N9B 3P4 , Canada.

Maleamate amidohydrolase (NicF) is a key enzyme in vitamin B metabolism that catalyzes the hydrolysis of maleamate to produce maleic acid and ammonia. Unlike most members from the amidohydrolase superfamily it does not require a metal ion. Here, we use multiscale computational enzymology to investigate the catalytic mechanism, substrate binding, oxyanion hole, and roles of key active site residues of NicF from . Read More

View Article and Full-Text PDF
September 2019

Predictors of outcome of subdural empyema in children.

Neurosurg Focus 2019 08;47(2):E17

Objective: The aim of this study was to report the etiology, clinical features, microbiology, surgical outcome, and predictors of outcome of spontaneous subdural empyema (SDE).

Methods: The authors conducted a retrospective study in a tertiary hospital. Children up to 18 years of age, with a diagnosis of SDE with infective etiology, were included in the present cohort. Read More

View Article and Full-Text PDF

Effect of silver diamine fluoride-potassium iodide and 2% chlorhexidine gluconate cavity cleansers on the bond strength and microleakage of resin-modified glass ionomer cement.

J Conserv Dent 2019 Mar-Apr;22(2):201-206

Dental Material, Manipal College of Dental Sciences, Manipal, Affiliated to Manipal Academy of Higher Education, Manipal, Karnataka, India.

Background: Disinfection of the prepared cavity can be a crucial step in the longevity of restorations. The objective of this study was to compare the antimicrobial action (AMA) of silver diamine fluoride-potassium iodide combination (SDF-KI) with 2% chlorhexidine gluconate (CHX) and to compare the alteration in bond strength and microleakage while using SDF-KI and CHX as cavity cleansers in resin-modified glass ionomer cement (RMGIC) restorations.

Materials And Methods: Samples were grouped as follows: Group 1: Polyacrylic acid (PAA), Group 2: CHX, Group 3: SDF-KI, and Group 4: Distilled water (CTRL). Read More

View Article and Full-Text PDF

Paediatric brain abscesses: a single centre experience.

Br J Neurosurg 2019 Oct 26;33(5):550-554. Epub 2019 May 26.

Department of Neurosurgery, University Hospitals Southampton NHS Foundation Trust , Southampton , UK.

Brain parenchymal abscesses are relatively infrequent but potentially serious infections in the paediatric population. Surgical intervention in addition to a prolonged administration of antibiotics is generally appropriate management. This study presents our centre's experience of managing such patients in the context of relevant literature. Read More

View Article and Full-Text PDF
October 2019

Cerebral Abscess caused by Novel Species: Streptococcus Pluranimalium.

J Coll Physicians Surg Pak 2018 Sep;28(9):S181-S183

Department of Radiology, Military Hospital, Rawalpindi.

Streptococcus pluranimalium, a gram-positive aerobic coccus, has been isolated primarily from several farm animals. The pathogenicity of this species is not well characterised either in animals or humans. As per the literature, cases of S. Read More

View Article and Full-Text PDF
September 2018

Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner.

Microbiome 2018 07 5;6(1):109. Epub 2018 Jul 5.

Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

Background: The gut microbiome has an important role in infant health and immune development and may be affected by early-life exposures. Maternal diet may influence the infant gut microbiome through vertical transfer of maternal microbes to infants during vaginal delivery and breastfeeding. We aimed to examine the association of maternal diet during pregnancy with the infant gut microbiome 6 weeks post-delivery in mother-infant dyads enrolled in the New Hampshire Birth Cohort Study. Read More

View Article and Full-Text PDF

Treatment and reconstruction of a complicated infected scalp squamous cell carcinoma with CNS invasion.

BMJ Case Rep 2018 Apr 28;2018. Epub 2018 Apr 28.

Department of Plastic and Reconstructive Surgery, Odense University Hospital, Odense, Denmark.

A 60-year-old male patient with a large infected cranial apex lesion was admitted with lethargy and mental status changes. The patient underwent evaluation with imaging studies, a skin biopsy, cultures with microscopy and a diagnostic burr hole. MRI and positron emission tomography/CT scan revealed a squamous cell carcinoma with ingrowth in the midline of the brain and subdural empyema infected with and High dose intravenous antibiotic treatment was initiated and the patient subsequently underwent a surgical resection of the carcinoma with a 1 cm margin of surrounding skin and skull. Read More

View Article and Full-Text PDF

Ultrasonic assisted synthesis with enhanced visible-light photocatalytic activity of NiO/AgVO nanocomposite and its antibacterial activity.

Ultrason Sonochem 2018 Jun 7;44:73-85. Epub 2018 Feb 7.

Madurai Kamaraj University, Madurai 625 021, Tamilnadu, India.

The NiO/AgVO nanocomposite photocatalysts were developed by ultrasonic assisted preparation method to study the photocatalytic activity under visible light irradiation. The samples were characterized by UV-DRS, XRD, FT-IR, XPS, SEM, EDX, TEM, EIS and BET analysis. The photocatalytic activity of NiO/AgVO nanocomposite for the photodegradation of 4-Nitro Phenol (4-NP) and Rose Bengal (RB) under visible light irradiation was studied and it is observed that the activity has been much higher than that of the pure AgVO. Read More

View Article and Full-Text PDF

Peptidoglycan Modification by the Catalytic Domain of Streptococcus pneumoniae OatA Follows a Ping-Pong Bi-Bi Mechanism of Action.

Biochemistry 2018 04 5;57(16):2394-2401. Epub 2018 Apr 5.

Department of Molecular & Cellular Biology , University of Guelph , Guelph , ON N1G 2W1 , Canada.

Streptococcus pneumoniae among other Gram-positive pathogens produces O-acetylated peptidoglycan using the enzyme OatA. This process occurs through the transfer of an acetyl group from a donor to the hydroxyl group of an acceptor sugar. While it has been established that this process involves the extracellular, catalytic domain of OatA ( SpOatA), mechanistic insight is still unavailable. Read More

View Article and Full-Text PDF

Marker-free method for accurate alignment between correlated light, cryo-light, and electron cryo-microscopy data using sample support features.

J Struct Biol 2018 01 4;201(1):46-51. Epub 2017 Nov 4.

Sanford-Burnham-Prebys Medical Discovery Institute, Bioinformatics and Structural Biology Program, La Jolla, CA, USA. Electronic address:

Combining fluorescence microscopy with electron cryo-tomography allows, in principle, spatial localization of tagged macromolecular assemblies and structural features within the cellular environment. To allow precise localization and scale integration between the two disparate imaging modalities, accurate alignment procedures are needed. Here, we describe a marker-free method for aligning images from light or cryo-light fluorescence microscopy and from electron cryo-microscopy that takes advantage of sample support features, namely the holes in the carbon film. Read More

View Article and Full-Text PDF
January 2018

In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA).

PLoS Pathog 2017 Oct 27;13(10):e1006667. Epub 2017 Oct 27.

Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.

The O-acetylation of the essential cell wall polymer peptidoglycan occurs in most Gram-positive bacterial pathogens, including species of Staphylococcus, Streptococcus and Enterococcus. This modification to peptidoglycan protects these pathogens from the lytic action of the lysozymes of innate immunity systems and, as such, is recognized as a virulence factor. The key enzyme involved, peptidoglycan O-acetyltransferase A (OatA) represents a particular challenge to biochemical study since it is a membrane associated protein whose substrate is the insoluble peptidoglycan cell wall polymer. Read More

View Article and Full-Text PDF
October 2017

Efficacy of Neem Extract and Three Antimicrobial Agents Incorporated into Tissue Conditioner in Inhibiting the Growth of and .

J Clin Diagn Res 2017 May 1;11(5):ZC97-ZC101. Epub 2017 May 1.

Dean and Professor and Head, Department of Conservative Dentistry, New Horizon Dental College and Research Centre, Bilaspur, Chhattisgarh, India.

Introduction: Denture stomatitis is an inflammatory condition which compromises the mucosal surface beneath dentures. The aetiology of denture stomatitis is usually multifactorial which varies from trauma from ill fitting denture to poor immune system. There are evidences that denture stomatitis is an outcome of multispecies biofilms that include and . Read More

View Article and Full-Text PDF

A case of multiple empyema caused by Streptococcus intermedius.

Auris Nasus Larynx 2017 Dec 29;44(6):745-748. Epub 2016 Nov 29.

Department of Otorhinolaryngology, Juntendo University, 3-1-3 bunkyou-ku honngo, Tokyo 113-8431, Japan.

We report the case of a patient with multiple empyema present throughout his body, including chronic sinusitis and chronic suppurative otitis media, as well as subsequent epidural empyema, all caused by Streptococcus intermedius. A 38-year-old man presented with chief complaints of headache, left ear discharge, and nasal congestion. Imaging studies revealed pansinusitis, soft tissue signs in the mastoid cells, and otitis media. Read More

View Article and Full-Text PDF
December 2017

Fixing a Hole: Preventing Pneumococcal Pneumonia by Vaccination.

Ger T Rijkers

Front Immunol 2016 13;7:349. Epub 2016 Sep 13.

Science Department, University College Roosevelt, Middelburg, Netherlands; Laboratory for Medical Microbiology and Immunology, St. Antonius Hospital, Nieuwegein, Netherlands.

View Article and Full-Text PDF
September 2016

Extrusion of barley and oat influence the fecal microbiota and SCFA profile of growing pigs.

Food Funct 2016 Feb;7(2):1024-32

Nofima, The Norwegian Institute of Food, Fisheries and Aquaculture Research, Osloveien 1, N-1430 Aas, Norway.

The effect of extrusion of barley and oat on the fecal microbiota and the formation of SCFA was evaluated using growing pigs as model system. The pigs were fed a diet containing either whole grain barley (BU), oat groat (OU), or their respective extruded samples (BE and OE). 454 pyrosequencing showed that the fecal microbiota of growing pigs was affected by both extrusion and grain type. Read More

View Article and Full-Text PDF
February 2016

Corticosteroid therapy and airflow obstruction influence the bronchial microbiome, which is distinct from that of bronchoalveolar lavage in asthmatic airways.

J Allergy Clin Immunol 2016 05 25;137(5):1398-1405.e3. Epub 2015 Nov 25.

Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill. Electronic address:

Background: The lung has a diverse microbiome that is modest in biomass. This microbiome differs in asthmatic patients compared with control subjects, but the effects of clinical characteristics on the microbial community composition and structure are not clear.

Objectives: We examined whether the composition and structure of the lower airway microbiome correlated with clinical characteristics of chronic persistent asthma, including airflow obstruction, use of corticosteroid medications, and presence of airway eosinophilia. Read More

View Article and Full-Text PDF

Comparative biology of the pentraxin protein family: evolutionarily conserved component of innate immune system.

Int Rev Cell Mol Biol 2015 27;316:1-47. Epub 2015 Feb 27.

Department of Molecular and Cellular Biology, University of California, Davis, CA, USA; Marine Biological Laboratory, Woods Hole, MA, USA. Electronic address:

The immune system is based on the actions of the collection of specialized immune defense cells and their secreted proteins and peptides that defend the host against infection by parasites. Parasites are organisms that live part or all of their lives in close physical association with the host and extract nutrients from the host and, by releasing toxins and virulence factors, cause disease with the potential for injury and premature death of that host. Parasites of the metazoa can be viruses, eubacteria, fungi, protozoans, and other metazoans. Read More

View Article and Full-Text PDF
December 2015

Streptococcus pluranimalium: A novel human pathogen?

Int J Surg Case Rep 2014 14;5(12):1242-6. Epub 2014 Nov 14.

Department of Emergency Medicine, Rashid Hospital Trauma Center, Dubai, United Arab Emirates.

Introduction: We present the first case of a subdural empyema caused by Streptococcus pluranimalium, in a healthy adolescent male as a possible complication of subclinical frontal sinusitis. Clinical features, diagnostic approach and management of subdural empyema are discussed.

Presentation Of Case: A 17-year-old male with a 2 day history of headache and nausea was referred to our Emergency Department (ED) as a case of possible meningitis. Read More

View Article and Full-Text PDF
December 2014

Dynamics of tongue microbial communities with single-nucleotide resolution using oligotyping.

Front Microbiol 2014 7;5:568. Epub 2014 Nov 7.

Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory Woods Hole, MA, USA ; Department of Microbiology, The Forsyth Institute Cambridge, MA, USA.

The human mouth is an excellent system to study the dynamics of microbial communities and their interactions with their host. We employed oligotyping to analyze, with single-nucleotide resolution, oral microbial 16S ribosomal RNA (rRNA) gene sequence data from a time course sampled from the tongue of two individuals, and we interpret our results in the context of oligotypes that we previously identified in the oral data from the Human Microbiome Project. Our previous work established that many of these oligotypes had dramatically different distributions between individuals and across oral habitats, suggesting that they represented functionally different organisms. Read More

View Article and Full-Text PDF
November 2014

Incomplete pneumolysin oligomers form membrane pores.

Open Biol 2014 Apr 23;4:140044. Epub 2014 Apr 23.

Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.

Pneumolysin is a member of the cholesterol-dependent cytolysin (CDC) family of pore-forming proteins that are produced as water-soluble monomers or dimers, bind to target membranes and oligomerize into large ring-shaped assemblies comprising approximately 40 subunits and approximately 30 nm across. This pre-pore assembly then refolds to punch a large hole in the lipid bilayer. However, in addition to forming large pores, pneumolysin and other CDCs form smaller lesions characterized by low electrical conductance. Read More

View Article and Full-Text PDF

Structure-based elucidation of the regulatory mechanism for aminopeptidase activity.

Acta Crystallogr D Biol Crystallogr 2013 Sep 15;69(Pt 9):1738-47. Epub 2013 Aug 15.

Department of Molecular Cell Biology, School of Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University, Suwon 440-746, Republic of Korea.

The specificity of proteases for the residues in and length of substrates is key to understanding their regulatory mechanism, but little is known about length selectivity. Crystal structure analyses of the bacterial aminopeptidase PepS, combined with functional and single-molecule FRET assays, have elucidated a molecular basis for length selectivity. PepS exists in open and closed conformations. Read More

View Article and Full-Text PDF
September 2013

Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2.

Nucleic Acids Res 2013 Jul 26;41(12):6347-59. Epub 2013 Apr 26.

Institut für Physikalische Biologie, Heinrich-Heine-Universität, Universitätsstr. 1, 40225 Düsseldorf, Germany.

The adaptive immunity of bacteria against foreign nucleic acids, mediated by CRISPR (clustered regularly interspaced short palindromic repeats), relies on the specific incorporation of short pieces of the invading foreign DNA into a special genomic locus, termed CRISPR array. The stored sequences (spacers) are subsequently used in the form of small RNAs (crRNAs) to interfere with the target nucleic acid. We explored the DNA-binding mechanism of the immunization protein Csn2 from the human pathogen Streptococcus agalactiae using different biochemical techniques, atomic force microscopic imaging and molecular dynamics simulations. Read More

View Article and Full-Text PDF

Central condylar displacement with brain abscess from chronic mandibular osteomyelitis.

Laryngoscope 2013 Jun 11;123(6):1369-73. Epub 2013 Jan 11.

Department of Otolaryngology, Upstate Medical University, Syracuse, New York, USA.

In this case report, we describe a unique long-term complication from undiagnosed mandibular osteomyelitis. A 53-year-old female who underwent a dental extraction complicated by chronic postoperative odontogenic infection and cutaneous parotid fistula formation 2 years earlier presented with acute mental status change, gradual unilateral facial nerve palsy (House-Brackmann score V), and nontraumatic dislocation of the condylar head into the middle cranial fossa. The patient's chronic mandibular osteomyelitis led to glenoid fossa erosion, middle cranial fossa penetration, and temporal lobe abscess formation. Read More

View Article and Full-Text PDF

Critical cell wall hole size for lysis in Gram-positive bacteria.

J R Soc Interface 2013 Mar 9;10(80):20120892. Epub 2013 Jan 9.

School of Biology, Georgia Institute of Technology, Atlanta, GA, USA.

Gram-positive bacteria can transport molecules necessary for their survival through holes in their cell wall. The holes in cell walls need to be large enough to let critical nutrients pass through. However, the cell wall must also function to prevent the bacteria's membrane from protruding through a large hole into the environment and lysing the cell. Read More

View Article and Full-Text PDF