38 results match your criteria hla-dr cd209


LILRA3 is related to monocyte-derived dendritic cell maturation and activation.

Iran J Basic Med Sci 2021 Feb;24(2):196-202

Department of Rheumatology, The Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, China.

Objectives: Previously we reported functional leukocyte immunoglobulin-like receptor A3 () leads to susceptibility and sub-phenotypes of several autoimmune diseases. LILRA3 levels in blood serum and CD14 monocytes enhanced in systemic lupus erythematosus and resulted in disease severity. However, the mechanism of LILRA3 in the pathogenesis of autoimmunity remains elusive. Read More

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February 2021

The modulation of mature dendritic cells from patients with type 1 diabetes using human periodontal ligament stem cells. An in-vitro study.

J Diabetes Metab Disord 2020 Dec 12;19(2):1037-1044. Epub 2020 Aug 12.

Cell Therapy Center, The University of Jordan, Amman, Jordan.

Objective: This in vitro study aimed to investigate whether human periodontal ligament stem cells isolated from impacted third molars can modify the maturation and phenotype of monocyte-derived dendritic cells pulsed with GAD-65 obtained from patients with type 1 diabetes.

Background: Human periodontal ligament stem cells (PDLSCs) have been found to display cell surface marker characteristics similar to bone marrow stromal stem cells (BMSSCs). The immunosuppressive effects on dendritic cells (DCs), T and B cells as well as their low immunogenicity allow the use of PDLSCs in stem cell therapies for autoimmune diseases including type 1 diabetes (T1D). Read More

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December 2020

Antibody combinations for optimized staining of macrophages in human lung tumours.

Scand J Immunol 2020 Jul 10;92(1):e12889. Epub 2020 May 10.

Tumor Immunology Lab, Department of Pathology, Rikshospitalet, Oslo University Hospital and University of Oslo, Oslo, Norway.

The analysis of tumour-associated macrophages (TAMs) has a high potential to predict cancer recurrence and response to immunotherapy. However, the heterogeneity of TAMs poses a challenge for quantitative and qualitative measurements. Here, we critically evaluated by immunohistochemistry and flow cytometry two commonly used pan-macrophage markers (CD14 and CD68) as well as some suggested markers for tumour-promoting M2 macrophages (CD163, CD204, CD206 and CD209) in human non-small cell lung cancer (NSCLC). Read More

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Monocyte-derived dendritic cells display a highly activated phenotype and altered function in patients with familial Mediterranean fever.

Clin Exp Immunol 2020 07 27;201(1):1-11. Epub 2020 Apr 27.

Department of Medical Oncology, Haematology, Clinical Immunology and Rheumatology, University Hospital Tübingen, Tübingen, Germany.

Dendritic cells (DCs) are sentinels of the immune system that bridge innate and adaptive immunity. By capturing antigens in peripheral tissue, processing and presenting them with concurrent expression of co-stimulatory molecules and cytokine secretion they control and modulate immune reactions. Through pattern recognition receptors, DCs sense molecules that are associated with infection or tissue damage, frequently resulting in the formation of inflammasomes upon intracellular stimulation. Read More

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CD209 monocyte-derived myeloid dendritic cells were increased in patients with leukemic cutaneous T-cell lymphoma undergoing extracorporeal photopheresis via the CELLEX system.

Photodermatol Photoimmunol Photomed 2020 Jul 16;36(4):290-298. Epub 2020 Apr 16.

Department of Dermatology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Background/purpose: We previously reported that myeloid dendritic cells (mDC) were increased in patients with leukemic cutaneous T-cell lymphoma (L-CTCL) following extracorporeal photopheresis (ECP) using the Therakos UVAR XTS system. We now assessed monocyte-derived mDCs (Mo-DCs) in L-CTCL patients treated with the CELLEX photopheresis system. CD209, a transmembrane receptor, was used to define Mo-DCs. Read More

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Immunobiologically relevant level of aflatoxin B alters transcription of key functional immune genes, phagocytosis and survival of human dendritic cells.

Immunol Lett 2018 05 20;197:44-52. Epub 2018 Mar 20.

Department of Clinical Science, Faculty of Veterinary Medicine, and Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.

The effects of naturally occurring levels of aflatoxin (AF) B on the expression of key molecules and function of dendritic cells (DCs) were investigated on human monocyte-derived DCs (MDDCs) by cell culture, RT-qPCR, and flow cytometry. An environmentally relevant level of AFB remarkably impaired the phagocytic capacity of MDDCs. Furthermore, AFB significantly affected the transcript levels of some key functional genes in MDDCs. Read More

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In vitro maturation of monocyte-derived dendritic cells results in two populations of cells with different surface marker expression, independently of applied concentration of interleukin-4.

Int Immunopharmacol 2018 Apr 28;57:165-171. Epub 2018 Feb 28.

Jagiellonian University Medical College, Faculty of Medicine, Department of Pharmacology, Ul. Grzegórzecka 16, 31-531 Kraków, Poland.

Dendritic cells (DCs) play a crucial role in the development of adaptive immune response. Monocyte-derived dendritic cells (MDDCs) are generated in vitro to study DC biology and for use in immunotherapy. However, procedures to generate MDDCs vary and an impact this may have on their final phenotype is insufficiently studied. Read More

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Lenalidomide increases human dendritic cell maturation in multiple myeloma patients targeting monocyte differentiation and modulating mesenchymal stromal cell inhibitory properties.

Oncotarget 2017 Aug 23;8(32):53053-53067. Epub 2017 May 23.

Department of Medicine and Surgery, University of Parma, Parma, Italy.

The use of Lenalidomide (LEN), to reverse tumor-mediated immune suppression and amplify multiple myeloma-specific immunity is currently being explored. Particularly, LEN effects on dendritic cells (DCs) are still unclear. In this study, we investigated the potential effect of LEN on DC differentiation and activity. Read More

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Immunomodelling Characteristics of Mature Dendritic Cells Stimulated by Colon Cancer Cells Lysates.

Pol Przegl Chir 2015 Feb;87(2):71-82

Unlabelled: Application of cells with high TAA (tumor associated antigen) presentation potential seems to be crucial in neoplasia immunotherapy. Such feature is distributed in dendritic cells, which present peptides from processed TAA - MHC molecules complex to the T cells of a host. The aim of the study was to assess the influence of colon neoplasia tissue lysate on functioning of generated autologous DC's in the field of autologous CD4+ lymphocytes immunological response towards Th1/Th2 under in vitro environment together with comparison and assessment of DCs' immunosuppressive properties acquired from patients with colon cancer. Read More

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February 2015

Decrease in circulating dendritic cell precursors in patients with peripheral artery disease.

Mediators Inflamm 2015 15;2015:450957. Epub 2015 Apr 15.

Department of Internal Medicine I, Jena University Hospital, Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany.

Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Read More

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February 2016

Decreased regulatory T cells in vulnerable atherosclerotic lesions: imbalance between pro- and anti-inflammatory cells in atherosclerosis.

Mediators Inflamm 2015 15;2015:364710. Epub 2015 Jan 15.

Department of Internal Medicine I, Friedrich-Schiller-University, 07747 Jena, Germany.

Atherosclerosis is a chronic inflammatory disease of the arterial wall in which presentation of autoantigens by dendritic cells (DCs) leads to the activation of T cells. Anti-inflammatory cells like Tregs counterbalance inflammation in atherogenesis. In our study, human carotid plaque specimens were classified as stable (14) and unstable (15) according to established morphological criteria. Read More

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January 2016

The human fetal placenta promotes tolerance against the semiallogeneic fetus by inducing regulatory T cells and homeostatic M2 macrophages.

J Immunol 2015 Feb 5;194(4):1534-44. Epub 2015 Jan 5.

Clinical Immunology, Department of Clinical and Experimental Medicine, Linköping University, 581 85 Linköping, Sweden; Department of Clinical Immunology and Transfusion Medicine, Linköping University, 581 85 Linköping, Sweden.

A successful pregnancy requires that the maternal immune system is instructed to a state of tolerance to avoid rejection of the semiallogeneic fetal-placental unit. Although increasing evidence supports that decidual (uterine) macrophages and regulatory T cells (Tregs) are key regulators of fetal tolerance, it is not known how these tolerogenic leukocytes are induced. In this article, we show that the human fetal placenta itself, mainly through trophoblast cells, is able to induce homeostatic M2 macrophages and Tregs. Read More

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February 2015

Dynamic changes in maternal decidual leukocyte populations from first to second trimester gestation.

Placenta 2014 Dec 6;35(12):1027-34. Epub 2014 Oct 6.

Department of Physiology, Faculty of Medicine, University of Toronto, Toronto M5G 1E2, Canada; Research Centre for Women's and Infants Health, Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Toronto M5T 3H7, Canada; Department of Obstetrics and Gynecology, Faculty of Medicine, University of Toronto, Toronto M5G 1E2, Canada.

Introduction: Decidual leukocytes are critical to the development of the fetomaternal interface, regulating tolerance to the semi-allogeneic fetus and vascular transformation of the uterine spiral arteries. Despite the continuation of these processes beyond the first trimester of pregnancy, the second trimester has largely been unstudied, with investigation focusing on early gestation and term tissues. We sought to characterize changes in decidual leukocyte populations from first to second trimester. Read More

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December 2014

Indoleamine 2,3-dioxygenase-1 (IDO1) in human endometrial stromal cells induces macrophage tolerance through interleukin-33 in the progression of endometriosis.

Int J Clin Exp Pathol 2014 15;7(6):2743-57. Epub 2014 May 15.

Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Shanghai Medical School, Fudan University Shanghai, 200011, China ; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai, 200011, China.

In the peritoneal fluid, macrophages and their secretory cytokines are essential for endometriosis, but the factors that favor their involvement in the endometriosis-associated inflammatory response are still elusive. Given the anomalous expression of indoleamine 2,3-dioxygenase-1 (IDO1) in endometrial stromal cells (ESCs) and its potentially important roles in immune modulation, we aimed to determine the effects of IDO1 in ESCs on macrophages and the mechanism of those effects. Normal ESCs and ectopic ESCs transfected with the SD11-IDO1 shRNA (short hairpin RNA) or vector-only plasmid SD11 were subsequently co-cultured with peripheral blood (PB)-derived monocytes (PBMC)-driven macrophages directly and indirectly. Read More

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Systemic monocyte-derived dendritic cells and associated Th2 skewing in chronic rhinosinusitis.

Otolaryngol Head Neck Surg 2014 Feb 23;150(2):312-20. Epub 2013 Dec 23.

Department of Otolaryngology-Head & Neck Surgery, Medical University of South Carolina, Charleston, South Carolina, USA.

Introduction: Monocyte-derived dendritic cells (moDCs) are antigen-presenting cells capable of directing immune responses toward T-helper 1 (Th1) or T-helper 2 (Th2) phenotypes. The systemic profile of moDCs and their association with Th1/Th2 skewing in chronic rhinosinusitis (CRS) is unclear. The purpose of this study is to characterize circulating moDCs in controls, CRS without nasal polyps (CRSsNP), and CRS with nasal polyps (CRSwNP) and correlate moDCs with Th1/Th2 skewing, mucosal inflammation on computed tomography (CT), and quality of life (QoL). Read More

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February 2014

Interleukin-1β triggers the differentiation of macrophages with enhanced capacity to present mycobacterial antigen to T cells.

Immunology 2014 Feb;141(2):174-80

Division of Dermatology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.

The rapid differentiation of monocytes into macrophages (MΦ) and dendritic cells is a pivotal aspect of the innate immune response. Differentiation is triggered following recognition of microbial ligands that activate pattern recognition receptors or directly by pro-inflammatory cytokines. We demonstrate that interleukin-1β (IL-1β) induces the rapid differentiation of monocytes into CD209(+) MΦ, similar to activation via Toll-like receptor 2/1, but with distinct phenotypic and functional characteristics. Read More

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February 2014

[Immunophenotypic characteristic of dendritic cells in bronchial asthma in conditions of extract opisthorchis felineus in vitro].

Vestn Ross Akad Med Nauk 2013 (5):66-70

This work shows comparative characteristics of immunogenic properties of the extract of Opisthorchis felineus in different severity of asthma using antigen presenting dendritic cells in vitro. New data on the effect of Opisthorchis felineus on the expression of surface markers of dendritic cells (CD209, HLA-DR, CD83, CD86) were received. Pronounced expression of CD209, CD86 and HLA-DR on the surface of dendritic cells in mild and severe asthma compared with healthy individuals was shown. Read More

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September 2013

Decreased numbers of regulatory T cells are associated with human atherosclerotic lesion vulnerability and inversely correlate with infiltrated mature dendritic cells.

Atherosclerosis 2013 Sep 10;230(1):92-9. Epub 2013 Jul 10.

Department of Cardiology and Angiology, University Hospital Erlangen, Germany.

Purpose: Mature dendritic cells (DCs) play a crucial role in the inflammatory process within atherosclerotic lesions by stimulation of effector T cells, which can contribute to plaque instability. Interactions between DCs and regulatory T cells (Treg), which regulate immune response by attenuating acute inflammation, are postulated to be involved in the pathogenesis of autoimmune diseases. We investigated a possible correlation between infiltrated DCs and Treg in human atherosclerotic plaques. Read More

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September 2013

Distribution of antigen presenting cells in the human cornea: correlation of in vivo confocal microscopy and immunohistochemistry in different pathologic entities.

Curr Eye Res 2012 Nov 5;37(11):1012-8. Epub 2012 Jun 5.

Department of Ophthalmology, Ludwig-Maximilians-University Munich, Germany.

Purpose: The purpose of this study was to determine the quantity and distribution of antigen presenting cells (APC) in various inflammatory and non-inflammatory corneal diseases, comparing in vivo confocal microscopy (IVCM) and immunohistochemistry.

Material And Methods: Corneae of 41 eyes, composed of group 1 (status post herpes-keratitis), group 2 (keratoconus) and group 3 (graft rejection after keratoplasty) were investigated. IVCM was used preoperatively to assess the distribution and density of dendritic cells in the corneal center versus the paracentral area. Read More

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November 2012

Comparative analysis of the interaction of Helicobacter pylori with human dendritic cells, macrophages, and monocytes.

Infect Immun 2012 Aug 21;80(8):2724-34. Epub 2012 May 21.

Institute of Tropical Medicine and International Health, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Helicobacter pylori may cause chronic gastritis, gastric cancer, or lymphoma. Myeloid antigen-presenting cells (APCs) are most likely involved in the induction and expression of the underlying inflammatory responses. To study the interaction of human APC subsets with H. Read More

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Dendritic cells in human renal inflammation--Part II.

Nephron Exp Nephrol 2011 4;119(4):e91-8. Epub 2011 Nov 4.

Institute of Molecular Immunology, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany.

Dendritic cells (DCs) are bone marrow-derived professional antigen-presenting cells that act as master regulators of acquired and innate immune responses. Here, we review the available information on their role in human renal inflammation. In the 1980s and early 1990s, major histocompatibility complex class II antigen- (HLA-DR) positive DCs were first described in normal human kidneys and in the interstitium of kidneys from patients with glomerulonephritis. Read More

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A new organotypic model containing dermal-type macrophages.

Exp Dermatol 2011 Dec 2;20(12):1035-7. Epub 2011 Nov 2.

Human skin equivalents (SEs) are popular three-dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti-inflammatory dermal macrophages has been performed. Here, we show that monocyte-derived dermal-type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. Read More

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December 2011

A subpopulation of CD163-positive macrophages is classically activated in psoriasis.

J Invest Dermatol 2010 Oct 17;130(10):2412-22. Epub 2010 Jun 17.

Laboratory for Investigative Dermatology, Rockefeller University, New York, New York 10065, USA.

Macrophages are important cells of the innate immune system, and their study is essential to gain greater understanding of the inflammatory nature of psoriasis. We used immunohistochemistry and double-label immunofluorescence to characterize CD163(+) macrophages in psoriasis. Dermal macrophages were increased in psoriasis compared with normal skin and were identified by CD163, RFD7, CD68, lysosomal-associated membrane protein 2 (LAMP2), stabilin-1, and macrophage receptor with collagenous structure (MARCO). Read More

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October 2010

Emergence of dendritic cells in the myocardium after acute myocardial infarction - implications for inflammatory myocardial damage.

Int J Biomed Sci 2010 Mar;6(1):27-36

Clinic of Internal Medicine I, Department of Cardiology, University Hospital Jena, Jena, Germany;

Dendritic cells (DC) are crucial for T cell mediated immune responses. Recently, we observed a significant decrease in circulating myeloid DC precursors in patients with acute myocardial infarction (AMI). The aim of the present study was to investigate whether myeloid DC are present in infarcted myocardium. Read More

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Active in vitro reduction of antigen presenting cells in human corneal grafts using different chemokines.

Curr Eye Res 2010 Feb;35(2):176-83

Department of Ophthalmology, Innsbruck Medical University, Austria.

Background: For the fate of a graft the antigen presenting cells play an important role. Chemokines can lead to enhanced migration of these cells. We therefore investigated if fresh human corneas bear the chemokine receptor 7 (CCR7) and if its ligands can force the emigration of dendritic cells in an in vitro model. Read More

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February 2010

Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry.

Nat Protoc 2010 Feb 4;5(2):357-70. Epub 2010 Feb 4.

Juvenile Diabetes Research Foundation/Wellcome Trust Diabetes and Inflammation Laboratory, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.

We describe two modular protocols for immunostaining and multiparameter flow cytometric analysis of major human antigen-presenting cells (APCs; e.g., dendritic cells, monocytes and B lymphocytes) in minimally manipulated whole blood samples. Read More

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February 2010

Transient decrease in circulating dendritic cell precursors after acute stroke: potential recruitment into the brain.

Clin Sci (Lond) 2009 Oct 19;118(2):147-57. Epub 2009 Oct 19.

Clinic of Internal Medicine I, Department of Cardiology, University Hospital Jena, Jena, Germany.

The role of DCs (dendritic cells) as potent mediators of inflammation has not been sufficiently investigated in stroke. Therefore, in the present study, circulating mDCPs (myeloid DC precursors), pDCPs (plasmacytoid DCPs) and tDCPs (total DCPs) were analysed by flow cytometry in (i) healthy controls (n=29), (ii) patients with ACI-S (asymptomatic cerebral infarction stenosis; n=46), (iii) patients with TIA (transient ischaemic attack; n=39), (iv) patients with AIS (acute ischaemic stroke; n=73), and (v) patients with AHS (acute haemorrhagic stroke; n=31). The NIHSS (National Institutes of Health Stroke Scale) and infarction size on a CT (computer tomography) scan were evaluated after stroke. Read More

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October 2009

Increased expression of CD208 (DC-LAMP) in epidermal keratinocytes of psoriatic lesions.

J Dermatol 2009 Mar;36(3):144-9

Research Center for Medical Sciences, The Jikei University School of Medicine, Tokyo, Japan.

Psoriasis is a chronic inflammatory skin disease characterized by epidermal hyperproliferation and infiltration of inflammatory leukocytes. The aim of this study was to clarify the role of innate immunity involving dendritic cells (DC) and keratinocytes in psoriasis. We immunohistochemically examined the expression of DC markers such as CD1a, CD83, CD207 (Langerin), CD208 (DC-LAMP) and CD209 (DC-SIGN) in psoriatic skin and gamma-interferon (IFN-gamma)/12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated keratinocytes in vitro. Read More

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Ontogeny and phagocytic function of baboon lung dendritic cells.

Immunol Cell Biol 2009 Jul 17;87(5):419-27. Epub 2009 Feb 17.

Department of Pharmaceutical Sciences, University of Oklahoma Health Science Center, Oklahoma City, OK, USA.

Dendritic cells (DCs) are the most potent antigen-presenting cells, but the ontogeny and functions of lung DCs are not known during prenatal period. Here, we isolated lung DC population from fetal (125-175 days of gestation age) and adult baboons. The cells were stained with fluorochrome-conjugated-HLA-DP, DQ, DR, CD1a, CD11c, CD14, CD40, CD80, CD86, CD209, CMKLR1, ILT7-specific antibodies, and staining was analyzed by flow cytometry. Read More

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