92 results match your criteria hk-ii prevents


Sodium hydrosulfide reverses β-microglobulin-induced depressive-like behaviors of male Sprague-Dawley rats: involving improvement of synaptic plasticity and enhancement of Warburg effect in hippocampus.

Behav Brain Res 2021 Sep 6:113562. Epub 2021 Sep 6.

Institute of Neurology, the First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, P. R. China; Institute of Neuroscience, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, P. R. China. Electronic address:

Background: Our previous works demonstrated that β2-microglobulin (β2m), a systemic pro-aging factor, induce depressive-like behaviors. Hydrogen sulfide (HS) is identified as a potential target for treatment of depression. The aim of the present work is to explore whether HS antagonizes β2m-induced depressive-like behaviors and the underlying mechanisms. Read More

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September 2021

The effects of low-intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose-rich diet.

J Food Biochem 2021 Sep 8:e13930. Epub 2021 Sep 8.

Laboratory for Molecular Biology and Endocrinology, Vinca Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.

We previously reported that low-intensity exercise prevented cardiac insulin resistance induced by a fructose-rich diet (FRD). To examine whether low-intensity exercise could prevent the disturbances of key molecules of cardiac glucose metabolism induced by FRD in male and ovariectomized (ovx) female rats, animals were exposed to 10% fructose solution (SF) or underwent both fructose diet and exercise (EF). Exercise prevented a decrease in cardiac GSK-3β phosphorylation induced by FRD in males (p < . Read More

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September 2021

Natural product triptolide induces GSDME-mediated pyroptosis in head and neck cancer through suppressing mitochondrial hexokinase-ΙΙ.

J Exp Clin Cancer Res 2021 Jun 9;40(1):190. Epub 2021 Jun 9.

Hunan Provincial Cancer Hospital and Cancer Hospital Affiliated to Xiangya Medical School, Central South University, Tongzipo Road, Changsha, 410013, Hunan, China.

Background: Pyroptosis is a lytic cell death form executed by gasdermins family proteins. Induction of tumor pyroptosis promotes anti-tumor immunity and is a potential cancer treatment strategy. Triptolide (TPL) is a natural product isolated from the traditional Chinese herb which possesses potent anti-tumor activity in human cancers. Read More

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Current Drug Treatment Strategies for Atrial Fibrillation and TASK-1 Inhibition as an Emerging Novel Therapy Option.

Front Pharmacol 2021 4;12:638445. Epub 2021 Mar 4.

Department of Cardiology, University of Heidelberg, Heidelberg, Germany.

Atrial fibrillation (AF) is the most common sustained arrhythmia with a prevalence of up to 4% and an upwards trend due to demographic changes. It is associated with an increase in mortality and stroke incidences. While stroke risk can be significantly reduced through anticoagulant therapy, adequate treatment of other AF related symptoms remains an unmet medical need in many cases. Read More

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NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.

Nat Immunol 2021 02 4;22(2):193-204. Epub 2021 Jan 4.

Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Metabolic reprograming toward aerobic glycolysis is a pivotal mechanism shaping immune responses. Here we show that deficiency in NF-κB-inducing kinase (NIK) impairs glycolysis induction, rendering CD8 effector T cells hypofunctional in the tumor microenvironment. Conversely, ectopic expression of NIK promotes CD8 T cell metabolism and effector function, thereby profoundly enhancing antitumor immunity and improving the efficacy of T cell adoptive therapy. Read More

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February 2021

Sequential activation of transcriptional repressors promotes progenitor commitment by silencing stem cell identity genes.

Elife 2020 11 26;9. Epub 2020 Nov 26.

Life Sciences Institute, University of Michigan, Ann Arbor, United States.

Stem cells that indirectly generate differentiated cells through intermediate progenitors drives vertebrate brain evolution. Due to a lack of lineage information, how stem cell functionality, including the competency to generate intermediate progenitors, becomes extinguished during progenitor commitment remains unclear. Type II neuroblasts in fly larval brains divide asymmetrically to generate a neuroblast and a progeny that commits to an intermediate progenitor (INP) identity. Read More

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November 2020

SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.

Theranostics 2020 23;10(25):11775-11793. Epub 2020 Sep 23.

Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan.

Urothelial carcinoma (UC), including upper tract urothelial carcinoma (UTUC) and urinary bladder urothelial carcinoma (UBUC), is a common malignant disease in developed countries. Oncogenic metabolic lesions have been associated with UC development. Using data mining, a series of studies were performed to study the involvement of SLC14A1 in UC specimens, animal models and UC-derived cell lines. Read More

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Cardiac metabolism as a driver and therapeutic target of myocardial infarction.

J Cell Mol Med 2020 06 8;24(11):5937-5954. Epub 2020 May 8.

Department of Medicine, University of Cambridge, Cambridge, UK.

Reducing infarct size during a cardiac ischaemic-reperfusion episode is still of paramount importance, because the extension of myocardial necrosis is an important risk factor for developing heart failure. Cardiac ischaemia-reperfusion injury (IRI) is in principle a metabolic pathology as it is caused by abruptly halted metabolism during the ischaemic episode and exacerbated by sudden restart of specific metabolic pathways at reperfusion. It should therefore not come as a surprise that therapy directed at metabolic pathways can modulate IRI. Read More

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Retinoic acid receptor-beta prevents cisplatin-induced proximal tubular cell death.

Biochim Biophys Acta Mol Basis Dis 2020 07 8;1866(7):165795. Epub 2020 Apr 8.

Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain. Electronic address:

Cisplatin's toxicity in renal tubular epithelial cells limits the therapeutic efficacy of this antineoplastic drug. In cultured human proximal tubular HK-2 cells (PTC) a prostaglandin uptake transporter (PGT)-dependent increase in intracellular prostaglandin E (iPGE) mediates cisplatin's toxicity (i.e. Read More

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Efficient, D-glucose insensitive, growth on D-xylose by an evolutionary engineered Saccharomyces cerevisiae strain.

FEMS Yeast Res 2019 12;19(8)

Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology (GBB), University of Groningen, Zernike Institute for Advanced Materials and Kluyver Centre for Genomics of Industrial Fermentation, Nijenborgh 7, 9747AG, Groningen, The Netherlands.

Optimizing D-xylose consumption in Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. An evolutionary engineering approach was used to elevate D-xylose consumption in a xylose-fermenting S. cerevisiae strain carrying the D-xylose-specific N367I mutation in the endogenous chimeric Hxt36 hexose transporter. Read More

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December 2019

Effective glucose metabolism maintains low intracellular glucose in airway epithelial cells after exposure to hyperglycemia.

Am J Physiol Cell Physiol 2019 11 21;317(5):C983-C992. Epub 2019 Aug 21.

Institute for Infection and Immunity, St. George's University of London, London, United Kingdom.

The airway epithelium maintains differential glucose concentrations between the airway surface liquid (ASL, ~0.4 mM) and the blood/interstitium (5-6 mM), which is important for defense against infection. Glucose primarily moves from the blood to the ASL via paracellular movement, down its concentration gradient, across the tight junctions. Read More

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November 2019

Rapid radiochemical filter paper assay for determination of hexokinase activity and affinity for glucose-6-phosphate.

J Appl Physiol (1985) 2019 09 11;127(3):661-667. Epub 2019 Jul 11.

Department of Physical Performance, Norwegian School of Sports Sciences, Oslo, Norway.

Glucose phosphorylation by hexokinase (HK) is a rate-limiting step in glucose metabolism. Regulation of HK includes feedback inhibition by its product glucose-6-phosphate (G6P) and mitochondria binding. HK affinity for G6P is difficult to measure because its natural product (G6P) inhibits enzyme activity. Read More

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September 2019

Inorganic nitrate, hypoxia, and the regulation of cardiac mitochondrial respiration-probing the role of PPARα.

FASEB J 2019 06 14;33(6):7563-7577. Epub 2019 Mar 14.

Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge, United Kingdom.

Dietary inorganic nitrate prevents aspects of cardiac mitochondrial dysfunction induced by hypoxia, although the mechanism is not completely understood. In both heart and skeletal muscle, nitrate increases fatty acid oxidation capacity, and in the latter case, this involves up-regulation of peroxisome proliferator-activated receptor (PPAR)α expression. Here, we investigated whether dietary nitrate modifies mitochondrial function in the hypoxic heart in a PPARα-dependent manner. Read More

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RAAS inhibitors directly reduce diabetes-induced renal fibrosis via growth factor inhibition.

J Physiol 2019 01 2;597(1):193-209. Epub 2018 Nov 2.

MTA-SE "Lendület" Diabetes Research Group, Hungarian Academy of Sciences and Semmelweis University, Budapest, Hungary.

Key Points: Increased activation of the renin-angiotensin-aldosterone system (RAAS) and elevated growth factor production are of crucial importance in the development of renal fibrosis leading to diabetic kidney disease. The aim of this study was to provide evidence for the antifibrotic potential of RAAS inhibitor (RAASi) treatment and to explore the exact mechanism of this protective effect. We found that RAASi ameliorate diabetes-induced renal interstitial fibrosis and decrease profibrotic growth factor production. Read More

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January 2019

Vacuolar sucrose cleavage prevents limitation of cytosolic carbohydrate metabolism and stabilizes photosynthesis under abiotic stress.

FEBS J 2018 11 28;285(21):4082-4098. Epub 2018 Sep 28.

Department Biology I, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.

Stabilization of central carbohydrate metabolism plays a key role in plant stress response. Carbohydrates are substrate for numerous metabolic and stress-responsive reactions and have been shown to be involved in diverse signalling processes on a whole-plant level. Regulation of enzymatic sucrose synthesis and degradation is well-known to be central to many stress-related processes as it significantly impacts stress tolerance. Read More

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November 2018

Inhibition of the oxidative stress-induced miR-125b protects glucose metabolic disorders of human retinal pigment epithelium (RPE) cells.

Cell Mol Biol (Noisy-le-grand) 2018 Mar 31;64(4):1-5. Epub 2018 Mar 31.

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, 201400, China.

The dysfunction of retinal pigment epithelium (RPE) with aging leads to age-related macular degeneration (AMD). Oxidative stress has been demonstrated as one of the causes of retinal pathological conditions. This study was conducted to investigate the mechanism of hydrogen peroxide (H2O2) induced human retinal pigment epithelial (RPE) cell dysfunction. Read More

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Hepatic glucose-6-phosphatase-α deficiency leads to metabolic reprogramming in glycogen storage disease type Ia.

Biochem Biophys Res Commun 2018 04 14;498(4):925-931. Epub 2018 Mar 14.

Section on Cellular Differentiation, Division of Translational Medicine, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address:

Glycogen storage disease type Ia (GSD-Ia) is caused by a deficiency in glucose-6-phosphatase-α (G6Pase-α or G6PC), a key enzyme in endogenous glucose production. This autosomal recessive disorder is characterized by impaired glucose homeostasis and long-term complications of hepatocellular adenoma/carcinoma (HCA/HCC). We have shown that hepatic G6Pase-α deficiency-mediated steatosis leads to defective autophagy that is frequently associated with carcinogenesis. Read More

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Resveratrol, a natural polyphenol, prevents chemotherapy-induced cognitive impairment: Involvement of cytokine modulation and neuroprotection.

Neurobiol Dis 2018 06 10;114:164-173. Epub 2018 Mar 10.

School of Chinese Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China. Electronic address:

Chemotherapy-induced cognitive impairment, also known as "chemobrain," is a common side effect. The purpose of this study was to examine whether resveratrol, a natural polyphenol that has nootropic effects, could prevent chemobrain and its underlying mechanisms. Mice received three injections of docetaxel, adriamycin, and cyclophosphamide (DAC) in combination, a common chemotherapy regimen, at two-day intervals within one week. Read More

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Loss of ABHD5 promotes the aggressiveness of prostate cancer cells.

Sci Rep 2017 10 12;7(1):13021. Epub 2017 Oct 12.

Department of Pathology, Wayne State University, Detroit, MI, 48201, USA.

The accumulation of neutral lipids in intracellular lipid droplets has been associated with the formation and progression of many cancers, including prostate cancer (PCa). Alpha-beta Hydrolase Domain Containing 5 (ABHD5) is a key regulator of intracellular neutral lipids that has been recently identified as a tumor suppressor in colorectal cancer, yet its potential role in PCa has not been investigated. Through mining publicly accessible PCa gene expression datasets, we found that ABHD5 gene expression is markedly decreased in metastatic castration-resistant PCa (mCRPC) samples. Read More

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October 2017

Chlamydia trachomatis Prevents Apoptosis Via Activation of PDPK1-MYC and Enhanced Mitochondrial Binding of Hexokinase II.

EBioMedicine 2017 Sep 5;23:100-110. Epub 2017 Aug 5.

Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany. Electronic address:

The intracellular human bacterial pathogen Chlamydia trachomatis pursues effective strategies to protect infected cells against death-inducing stimuli. Here, we show that Chlamydia trachomatis infection evokes 3-phosphoinositide-dependent protein kinase-1 (PDPK1) signaling to ensure the completion of its developmental cycle, further leading to the phosphorylation and stabilization of MYC. Using biochemical approaches and imaging we demonstrate that Chlamydia-induced PDPK1-MYC signaling induces host hexokinase II (HKII), which becomes enriched and translocated to the mitochondria. Read More

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September 2017

SOCS1 is a negative regulator of metabolic reprogramming during sepsis.

JCI Insight 2017 Jul 6;2(13). Epub 2017 Jul 6.

Department Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana.

Sepsis can induce an overwhelming systemic inflammatory response, resulting in organ damage and death. Suppressor of cytokine signaling 1 (SOCS1) negatively regulates signaling by cytokine receptors and Toll-like receptors (TLRs). However, the cellular targets and molecular mechanisms for SOCS1 activity during polymicrobial sepsis are unknown. Read More

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VDAC in cancer.

Authors:
N M Mazure

Biochim Biophys Acta Bioenerg 2017 Aug 7;1858(8):665-673. Epub 2017 Mar 7.

Institute for Research on Cancer and Aging, Nice (IRCAN), CNRS UMR7284, INSERM U1081, University of Nice, France; CNRS GDR 3697 Micronit, France. Electronic address:

The voltage-dependent anion channel (VDAC) is a pore located at the outer membrane of the mitochondrion. It allows the entry and exit of numerous ions and metabolites between the cytosol and the mitochondrion. Flux through the pore occurs in an active way: first, it depends on the open or closed state and second, on the negative or positive charges of the different ion species passing through the pore. Read More

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HK2 Recruitment to Phospho-BAD Prevents Its Degradation, Promoting Warburg Glycolysis by Theileria-Transformed Leukocytes.

ACS Infect Dis 2017 03 24;3(3):216-224. Epub 2017 Jan 24.

Inserm U1016, CNRS UMR8104, Cochin Institute , Paris 75014 France.

Theileria annulata infects bovine leukocytes, transforming them into invasive, cancer-like cells that cause the widespread disease called tropical theileriosis. We report that in Theileria-transformed leukocytes hexokinase-2 (HK2) binds to B cell lymphoma-2-associated death promoter (BAD) only when serine (S) 155 in BAD is phosphorylated. We show that HK2 recruitment to BAD is abolished by a cell-penetrating peptide that acts as a nonphosphorylatable BAD substrate that inhibits endogenous S155 phosphorylation, leading to complex dissociation and ubiquitination and degradation of HK2 by the proteasome. Read More

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Wnt Signaling Prevents the Aβ Oligomer-Induced Mitochondrial Permeability Transition Pore Opening Preserving Mitochondrial Structure in Hippocampal Neurons.

PLoS One 2017 6;12(1):e0168840. Epub 2017 Jan 6.

Centro de Envejecimiento y Regeneración (CARE), Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.

Alzheimer's disease (AD) is a neurodegenerative disorder mainly known for synaptic impairment and neuronal cell loss, affecting memory processes. Beside these damages, mitochondria have been implicated in the pathogenesis of AD through the induction of the mitochondrial permeability transition pore (mPTP). The mPTP is a non-selective pore that is formed under apoptotic conditions, disturbing mitochondrial structure and thus, neuronal viability. Read More

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APPL1 prevents pancreatic beta cell death and inflammation by dampening NFκB activation in a mouse model of type 1 diabetes.

Diabetologia 2017 03 23;60(3):464-474. Epub 2016 Dec 23.

State Key Laboratory of Pharmaceutical Biotechnology, The University of Hong Kong, L8, 21 Sassoon Road, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China.

Aims/hypothesis: Beta cell inflammation and demise is a feature of type 1 diabetes. The insulin-sensitising molecule 'adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1' (APPL1), which contains an NH-terminal Bin/Amphiphysin/Rvs domain, a central pleckstrin homology domain and a COOH-terminal phosphotyrosine-binding domain, has been shown to modulate inflammatory response in various cell types but its role in regulating beta cell mass and inflammation in type 1 diabetes remains unknown. Thus, we investigated whether APPL1 prevents beta cell apoptosis and inflammation in diabetes. Read More

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The HK2 Dependent "Warburg Effect" and Mitochondrial Oxidative Phosphorylation in Cancer: Targets for Effective Therapy with 3-Bromopyruvate.

Molecules 2016 Dec 15;21(12). Epub 2016 Dec 15.

Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego Street, 51-148 Wroclaw, Poland.

This review summarizes the current state of knowledge about the metabolism of cancer cells, especially with respect to the "Warburg" and "Crabtree" effects. This work also summarizes two key discoveries, one of which relates to hexokinase-2 (HK2), a major player in both the "Warburg effect" and cancer cell immortalization. The second discovery relates to the finding that cancer cells, unlike normal cells, derive as much as 60% of their ATP from glycolysis via the "Warburg effect", and the remaining 40% is derived from mitochondrial oxidative phosphorylation. Read More

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December 2016

Multivariate analysis and molecular interaction of curcumin with PPARγ in high fructose diet induced insulin resistance in rats.

Springerplus 2016 6;5(1):1732. Epub 2016 Oct 6.

Molecular Physiology Lab, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Rajiv Gandhi Salai, Kelambakkam, Tamil Nadu 603103 India.

To investigate the effect of curcumin on the multivariate and docking analysis on peroxisome proliferator activated receptor-γ, the rats were fed with high fructose diet (Group 2) to induce insulin resistance and curcumin was co-administered orally (Group 4) for a period of 8 weeks and measured the biochemical parameters in blood, kidney and liver tissues. The results showed a significant (p ≤ 0.05) increase in the level of creatinine, glucose, insulin, low density lipoprotein, total cholesterol, triglyceride, urea, uric acid, very low density lipoprotein and decreased albumin, high density lipoprotein and total protein level in the blood of Group 2 when compared with Group 1 control rats. Read More

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October 2016

Hexokinase I N-terminal based peptide prevents the VDAC1-SOD1 G93A interaction and re-establishes ALS cell viability.

Sci Rep 2016 10 10;6:34802. Epub 2016 Oct 10.

Department of Biological, Geological and Environmental Sciences, Section of Biochemistry and Molecular Biology, University of Catania, Italy.

Superoxide Dismutase 1 mutants associate with 20-25% of familial Amyotrophic Lateral Sclerosis (ALS) cases, producing toxic aggregates on mitochondria, notably in spinal cord. The Voltage Dependent Anion Channel isoform 1 (VDAC1) in the outer mitochondrial membrane is a docking site for SOD1 G93A mutant in ALS mice and the physiological receptor of Hexokinase I (HK1), which is poorly expressed in mouse spinal cord. Our results demonstrate that HK1 competes with SOD1 G93A for binding VDAC1, suggesting that in ALS spinal cord the available HK1-binding sites could be used by SOD1 mutants for docking mitochondria, producing thus organelle dysfunction. Read More

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October 2016

Application of anchoring stitch prevents rectal prolapse in laparoscopic assisted anorectal pullthrough.

J Pediatr Surg 2016 Dec 17;51(12):2113-2116. Epub 2016 Sep 17.

Division of Paediatric Surgery, Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Hong Kong. Electronic address:

Background: Rectal prolapse has been reported after laparoscopic assisted anorectal pullthrough in children with anorectal malformation. We report our clinical outcome and study the application of an anchoring stitch to tack the rectum to the presacral fascia and the occurrence of rectal prolapse.

Material And Methods: A retrospective review of all children who had undergone laparoscopic assisted anorectal pullthrough for anorectal malformation from 2000 to 2015 was performed. Read More

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December 2016

Complement, Kinins, and Hereditary Angioedema: Mechanisms of Plasma Instability when C1 Inhibitor is Absent.

Clin Rev Allergy Immunol 2016 Oct;51(2):207-15

Medical University of South Carolina, Charleston, SC, USA.

Plasma of patients with types I and II hereditary angioedema is unstable if incubated in a plastic (i.e., inert) vessel at 37 °C manifested by progressively increasing formation of bradykinin. Read More

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October 2016