4 results match your criteria hisb locus

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HisB as novel selection marker for gene targeting approaches in Aspergillus niger.

BMC Microbiol 2017 03 8;17(1):57. Epub 2017 Mar 8.

Institute of Biotechnology, Department of Applied and Molecular Microbiology, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355, Berlin, Germany.

Background: For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene integration and deletion in A. Read More

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[Cloning and inactivation of a chromosomal copy of the imidazole glycerophosphate dehydratase (HIS) gene from Hansenula polymorpha].

Mol Gen Mikrobiol Virusol 1991 Jul(7):25-8

A gene for imidazole glycerophosphate dehydratase (HIS) has been selected from the library of Hansenula polymorpha genes by complementation of Escherichia coli hisB mutations or his3 mutation of Saccharomyces cerevisiae. Inactivation of the gene in Hansenula polymorpha strain 48V, as well as Leu(-)-Leu+ conversion of phenotype and arising of antibiotic G418 resistance resulted from transformation of the strain by the linear DNA molecules of the cloned HIS-gene with the in vitro inserted LEU2 gene from Saccharomices cerevisiae and KmR gene. The Southern hybridization analysis of the Leuf+His- G418R clones representing 1-2% of transformants population together with the DNA analysis of the monospore clones from the hybrid Leu+His-G418R transformant tetrad and tester strain have revealed the locus specific nature of the clones. Read More

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Bifunctionality and polarized infidelity at the hisB locus of Aspergillus nidulans.

Mol Gen Genet 1984 ;193(2):332-9

The histidine (hisB) locus of Aspergillus nidulans is unusual in two ways. Firstly, it is bifunctional; besides coding for imidazole glycerol phosphate (IGP) dehydrase, it is required for the production of ascospores (fertility). It appears, therefore, to be partly homologous to the hisB locus of Salmonella typhimurium, which codes for IGP dehydrase and histidinol phosphate phosphatase. Read More

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Mapping of the 5-methyltryptophan resistance locus in Bacillus subtilis.

Authors:
S O Hoch

J Bacteriol 1974 Jan;117(1):315-7

The 5-methyltryptophan resistance locus (mtr) in Bacillus subtilis, which leads to constitutive production of the tryptophan enzymes, has been mapped on the chromosome. The order of loci is ser-1-mtr-aroF-aroB-trp-hisB. Read More

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January 1974
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