56 results match your criteria hisb gene

Multiple Argonaute family genes contribute to the siRNA-mediated RNAi pathway in Locusta migratoria.

Pestic Biochem Physiol 2020 Nov 12;170:104700. Epub 2020 Sep 12.

Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address:

Argonautes (Ago) are important core proteins in RNA interference (RNAi) pathways of eukaryotic cells. Generally, it is thought that Ago1, Ago2 and Ago3 are involved in the miRNA (microRNA), siRNA (small interfering RNA) and piRNA (Piwi-interacting RNA)-mediated RNAi pathways, respectively. As a main component of the RNA-induced silencing complex (RISC), Ago2 plays an indispensable role in using siRNA to recognize and cut target messenger RNAs resulting in suppression of transcript levels, but the contributions of Ago1 and Ago3 to the siRNA-mediated RNAi pathway remain to be explored in many insect species. Read More

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November 2020

The Role of Gene Elongation in the Evolution of Histidine Biosynthetic Genes.

Microorganisms 2020 May 13;8(5). Epub 2020 May 13.

Department of Biology, University of Florence, 50019 Sesto Fiorentino, Italy.

Gene elongation is a molecular mechanism consisting of an in-tandem duplication of a gene and divergence and fusion of the two copies, resulting in a gene constituted by two divergent paralogous modules. The aim of this work was to evaluate the importance of gene elongation in the evolution of histidine biosynthetic genes and to propose a possible evolutionary model for some of them. Concerning the genes and , which code for two homologous (β/α)-barrels, it has been proposed that the two extant genes could be the result of a cascade of gene elongation/domain shuffling events starting from an ancestor gene coding for just one (β/α) module. Read More

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Identification of Genetic Features for Attenuation of Two Enteritidis Vaccine Strains and Differentiation of These From Wildtype Isolates Using Whole Genome Sequencing.

Front Vet Sci 2019 18;6:447. Epub 2019 Dec 18.

Department of Bacteriology, Animal and Plant Health Agency, Addlestone, United Kingdom.

Enteritidis is a major cause of salmonellosis worldwide and more than 80% of outbreaks investigated in Europe have been associated with the consumption of poorly cooked eggs or foods containing raw eggs. Vaccination has been proven to be one of the most important measures to control Enteritidis infections in poultry farms as it can decrease colonization of the reproductive organs and intestinal tract of laying hens, thereby reducing egg contamination. Differentiation of live vaccine from field or wild type . Read More

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December 2019

PA0335, a Gene Encoding Histidinol Phosphate Phosphatase, Mediates Histidine Auxotrophy in .

Appl Environ Microbiol 2020 02 18;86(5). Epub 2020 Feb 18.

Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an, China

The biosynthesis of histidine, a proteinogenic amino acid, has been extensively studied due to its importance in bacterial growth and survival. Histidinol-phosphate phosphatase (Hol-Pase), which is responsible for the penultimate step of histidine biosynthesis, is generally the last enzyme to be characterized in many bacteria because its origin and evolution are more complex compared to other enzymes in histidine biosynthesis. However, none of the enzymes in histidine biosynthesis, including Hol-Pase, have been characterized in , which is an important opportunistic Gram-negative pathogen that can cause serious human infections. Read More

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February 2020

In vitro activity and In vivo efficacy of Isoliquiritigenin against Staphylococcus xylosus ATCC 700404 by IGPD target.

PLoS One 2019 20;14(12):e0226260. Epub 2019 Dec 20.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China.

Staphylococcus xylosus (S. xylosus) is a type of coagulase-negative Staphylococcus, which was previously considered as non-pathogenic. However, recent studies have linked it with cases of mastitis in cows. Read More

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Library Selection with a Randomized Repertoire of (βα)-Barrel Enzymes Results in Unexpected Induction of Gene Expression.

Biochemistry 2019 10 7;58(41):4207-4217. Epub 2019 Oct 7.

Institute of Biophysics and Physical Biochemistry , University of Regensburg , Universitätsstrasse 31 , D-93053 Regensburg , Germany.

The potential of the frequently encountered (βα)-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection . For this purpose, the genes encoding 52 different phosphate-binding (βα)-barrel proteins were subjected to error-prone PCR and cloned into an expression plasmid. The resulting mixed repertoire was used to transform different auxotrophic strains, each lacking an enzyme with a phosphate-containing substrate. Read More

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October 2019

CRKII overexpression promotes the proliferation, migration and invasion potential of murine hepatocarcinoma Hca-P cells.

Oncol Lett 2019 Jun 27;17(6):5169-5174. Epub 2019 Mar 27.

Department of Biochemistry, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.

Lymphatic metastasis is a major mechanism of tumor metastasis. The present study aimed to investigate the association of CRKII, a member of the CRK family, with the malignant behaviors of a murine hepatocarcinoma Hca-P cell line, with a lymph node metastatic rate of ~25%. Total mRNA was extracted from Hca-P cells, and then the murine CRKII gene was amplified by polymerase chain reaction and cloned into the pEASY-blunt cloning vector. Read More

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Histidine Metabolism and IGPD Play a Key Role in Cefquinome Inhibiting Biofilm Formation of .

Front Microbiol 2018 5;9:665. Epub 2018 Apr 5.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

() is an AT-rich and coagulase-negative (CNS). It is normally regarded as non-pathogenic, however, recent studies have demonstrated that it is related to human opportunistic infections and bovine mastitis. In addition, strains have the ability to form biofilm. Read More

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Insights Into the Roles of Two Genes of the Histidine Biosynthesis Operon in Pathogenicity of Xanthomonas oryzae pv. oryzicola.

Phytopathology 2018 May 21;108(5):542-551. Epub 2018 Mar 21.

First, second, fourth, and ninth authors: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; third, fifth, sixth, seventh, and ninth authors: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China/Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, China; and eighth author: Department of Biological Sciences, University of South Carolina, Columbia.

Xanthomonas oryzae pv. oryzicola is an X. oryzae pathovar that causes bacterial leaf streak in rice. Read More

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HisB as novel selection marker for gene targeting approaches in Aspergillus niger.

BMC Microbiol 2017 03 8;17(1):57. Epub 2017 Mar 8.

Institute of Biotechnology, Department of Applied and Molecular Microbiology, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355, Berlin, Germany.

Background: For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene integration and deletion in A. Read More

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A protein constructed de novo enables cell growth by altering gene regulation.

Proc Natl Acad Sci U S A 2016 Mar 16;113(9):2400-5. Epub 2016 Feb 16.

Department of Chemistry, Princeton University, Princeton, NJ 08540

Recent advances in protein design rely on rational and computational approaches to create novel sequences that fold and function. In contrast, natural systems selected functional proteins without any design a priori. In an attempt to mimic nature, we used large libraries of novel sequences and selected for functional proteins that rescue Escherichia coli cells in which a conditionally essential gene has been deleted. Read More

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Histidine biosynthesis plays a crucial role in metal homeostasis and virulence of Aspergillus fumigatus.

Virulence 2016 05 6;7(4):465-76. Epub 2016 Feb 6.

a Division of Molecular Biology, Biocenter, Medical University of Innsbruck , Innsbruck , Austria.

Aspergillus fumigatus is the most prevalent airborne fungal pathogen causing invasive fungal infections in immunosuppressed individuals. The histidine biosynthetic pathway is found in bacteria, archaebacteria, lower eukaryotes, and plants, but is absent in mammals. Here we demonstrate that deletion of the gene encoding imidazoleglycerol-phosphate dehydratase (HisB) in A. Read More

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[MUC1-2VNTR DNA Vaccine Induces Immune Responses in Mouse Model with Multiple Myeloma].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2015 Oct;23(5):1366-9

Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China. E-mail:

Objective: To investigate the humoral and cellular immune responses induced by MUC1-2VNTR DNA vaccine in multiple myeloma (MM) tumor-bearing mice.

Methods: In vitro, multiple myeloma cells were transfected by plasmid pcDNA3.1-2VNTR/myc-hisB with Lipofectamine2000. Read More

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October 2015

Genetic manipulations of the hyperthermophilic piezophilic archaeon Thermococcus barophilus.

Appl Environ Microbiol 2014 Apr 31;80(7):2299-306. Epub 2014 Jan 31.

Université de Bretagne Occidentale, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes, Institut Universitaire Européen de la Mer, Plouzané, France.

In this study, we developed a gene disruption system for Thermococcus barophilus using simvastatin for positive selection and 5-fluoroorotic acid (5-FOA) for negative selection or counterselection to obtain markerless deletion mutants using single- and double-crossover events. Disruption plasmids carrying flanking regions of each targeted gene were constructed and introduced by transformation into wild-type T. barophilus MP cells. Read More

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Human ubiquitin C promoter based expression of erythropoietin in CHO K1 cell lines: a simple transfectants screening approach.

Anim Biotechnol 2013 ;24(3):198-209

Center for Biotechnology, Anna University, Guindy, Chennai 600025, India.

Erythropoietin (EPO), a glycoprotein hormone that regulates the production of erythrocytes in the human body, is of clinical importance in the treatment of anemia. Low expression levels of this recombinant hormone and time-consuming screening methods have made its commercial production expensive. Cloning of human EPO gene in a shuttle vector pUB6/V5-HisB driven by human ubiquitin C promoter and its transfection in CHO K1 cell lines by electroporation resulted in a moderate level of EPO expression. Read More

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December 2013

Substrate ambiguous enzymes within the Escherichia coli proteome offer different evolutionary solutions to the same problem.

Mol Biol Evol 2013 Sep 31;30(9):2001-12. Epub 2013 May 31.

Department of Biochemistry, Center for Fundamental and Applied Molecular Evolution, Rollins Research Center, Emory University School of Medicine, USA.

Many enzymes exhibit some catalytic promiscuity or substrate ambiguity. These weak activities do not affect the fitness of the organism under ordinary circumstances, but can serve as potential evolutionary precursors of new catalytic functions. We wondered whether different proteins with the same substrate ambiguous activity evolve differently under identical selection conditions. Read More

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September 2013

[Relationship of histidine auxotrophy of Acidovorax citrulli with pathogenicity].

Wei Sheng Wu Xue Bao 2011 Sep;51(9):1185-93

College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China.

Unlabelled: Acidovorax citrulli (Ac) is an important bacterium that occurs in watermelon, melon and other cucurbits. It mainly damages watermelon and melon, and can cause leaf blight, fruit rot, and even mortality.

Objective: To verify the relationship between defects in the synthesis of histidine and the pathogenicity of Ac. Read More

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September 2011

Purification and characterization of the subtilisin-like protease of Streptococcus suis that contributes to its virulence.

Vet Microbiol 2011 Mar 27;148(2-4):333-40. Epub 2010 Oct 27.

Groupe de Recherche en Écologie Buccale (GREB), Faculté de médecine dentaire, Université Laval, Quebec City, Quebec, Canada.

Streptococcus suis is a major swine pathogen that is responsible for severe infections such as meningitis, endocarditis, and septicemia. S. suis is also recognized as a zoonotic agent and expresses several virulence factors. Read More

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[Construction of human TAP1 expression vector and the effects of it on HLA-I expression in GES-1 cells in vitro].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2010 Apr;26(4):329-32

Lab Exp Pathology, Hebei Medical University, Shijiazhuang 050017, China.

Aim: To construct the human TAP1 expression vector and to evaluate its effects on HLA-I expression in GES-1 cells in vitro.

Methods: The human TAP1 expression vector (pcDNA3.1/V5-His-TAP1) was constructed by gene recombination technology. Read More

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Divergence of biochemical function in the HAD superfamily: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB).

Biochemistry 2010 Feb;49(6):1072-81

Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA.

D-Glycero-d-manno-heptose-1,7-bisphosphate phosphatase (GmhB) is a member of the histidinol-phosphate phosphatase (HisB) subfamily of the haloalkanoic acid dehalogenase (HAD) enzyme superfamily. GmhB supports two divergent biochemical pathways in bacteria: the d-glycero-d-manno-heptose-1alpha-GDP pathway (in S-layer glycoprotein biosynthesis) and the l-glycero-d-manno-heptose-1beta-ADP pathway (in lipid A biosynthesis). Herein, we report the comparative analysis of substrate recognition in selected GmhB orthologs. Read More

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February 2010

Structural determinants of substrate recognition in the HAD superfamily member D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) .

Biochemistry 2010 Feb;49(6):1082-92

Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118-2394, USA.

The haloalkanoic acid dehalogenase (HAD) enzyme superfamily is the largest family of phosphohydrolases. In HAD members, the structural elements that provide the binding interactions that support substrate specificity are separated from those that orchestrate catalysis. For most HAD phosphatases, a cap domain functions in substrate recognition. Read More

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February 2010

Structural, evolutionary and genetic analysis of the histidine biosynthetic "core" in the genus Burkholderia.

Gene 2009 Dec 13;448(1):16-28. Epub 2009 Aug 13.

Department of Evolutionary Biology, University of Florence, 50125 Florence, Italy.

In this work a detailed analysis of the structure, the expression and the organization of his genes belonging to the core of histidine biosynthesis (hisBHAF) in 40 newly determined and 13 available sequences of Burkholderia strains was carried out. Data obtained revealed a strong conservation of the structure and organization of these genes through the entire genus. The phylogenetic analysis showed the monophyletic origin of this gene cluster and indicated that it did not undergo horizontal gene transfer events. Read More

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December 2009

[Expression and antigenicity analysis of NTPase gene of Toxoplasma gondii].

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 2007 Dec;25(6):447-50

Wenzhou Medical College, Wenzhou 325035, China.

Objective: To clone and express prokaryotic recombinant plasmid of nucleoside triphosphate hydrolase (NTPase) gene of Toxoplasma gondii, and analyze its antigenicity.

Method: NTPase gene was amplified by PCR from RH strain of T. gondii and cloned into pGEM-T Easy vector. Read More

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December 2007

Stable expression of Hantavirus H8205 strain G1/IL-2 gene and immune protection of the fusion gene.

J Huazhong Univ Sci Technolog Med Sci 2007 Apr;27(2):124-7

Department of Pathogenetic Biology, School of Basic Medical Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing Hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. Read More

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Efficient silencing of the gene coding for the epsilon subunit of DNA polymerase III in Escherichia coli is triggered by antisense RNAs featuring stability in vivo.

FEMS Microbiol Lett 2007 May 2;270(2):277-83. Epub 2007 Mar 2.

Consorzio per lo Sviluppo dei Sistemi a Grande Interfase, Firenze, Italy.

The Escherichia coli gene dnaQ coding for the epsilon subunit of DNA polymerase III was suppressed in vivo via antisense RNAs. To this aim, different fragments of dnaQ were cloned in reverse orientation into the pBAD-HisB vector or into pT3T7, and the corresponding antisense RNAs were conditionally expressed in vivo. Antisense transcripts featuring fast hybridization in vitro with dnaQ mRNA but lacking stability in vivo did not suppress the target gene. Read More

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Molecular evolution of hisB genes.

J Mol Evol 2004 Feb;58(2):225-37

Dipartimento di Biologia Animale e Genetica, Via Romana 17-19, 1-50125, Firenze, Italy.

The sixth and eighth steps of histidine biosynthesis are catalyzed by an imidazole glycerol-phosphate (IGP) dehydratase (EC 4.2.1. Read More

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February 2004

Cloning and sequence analysis of the Candida utilis HIS3 gene.

Biomol Eng 2004 Jan;21(1):15-20

Center for Genetic Engineering and Biotechnology, Agriculture Division, 31 Ave /158 & 190, PO Box 6162, Havana 10600, Cuba.

A DNA fragment, carrying the Candida utilis HIS3 gene, has been isolated from a genomic DNA library by complementation of the E. coli hisB mutant. Its nucleotide sequence was determined and it predicts a single open reading frame of 675 bp (224 aa). Read More

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January 2004

The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages.

Mol Microbiol 2001 Nov;42(3):851-65

Department of Microbiology, New York University Medical Center, New York, NY 10016, USA.

In this work, we characterize genes in Mycobacterium tuberculosis that are regulated by IdeR (iron-dependent regulator), an iron-responsive DNA-binding protein of the DtxR family that has been shown to regulate iron acquisition in Mycobacterium smegmatis. To identify some of the genes that constitute the IdeR regulon, we searched the M. tuberculosis genome for promoter regions containing the consensus IdeR/DxR binding sequence. Read More

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November 2001

Expression of horizontally transferred gene clusters: activation by promoter-generating mutations.

Res Microbiol 2001 Jul-Aug;152(6):539-49

Dipartimento di Biologia Animale e Genetica, Università di Firenze, Florence, Italy.

The occurrence of promoter-generating mutations allowing the transcription of heterologous genes has been studied in a system based on the plasmid-mediated conjugal transfer of histidine biosynthetic genes from a donor bacterium (Azospirillum brasilense) into a heterologous Escherichia coli mutant population lacking histidine biosynthetic ability and initially unable to recognize the transcriptional signal of the introgressed gene(s). Under selective stressful conditions, His+ revertants accumulated in the E. coli His- culture. Read More

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February 2002

gmhX, a novel gene required for the incorporation of L-glycero-D-manno-heptose into lipooligosaccharide in Neisseria meningitidis.

Microbiology (Reading) 2001 Aug;147(Pt 8):2367-2377

Department of Veterans Affairs Medical Center, Atlanta, GA 30033, USA2.

Lipooligosaccharide (LOS) is a critical virulence factor of Neisseria meningitidis. A Tn916 insertion mutant, designated 469, was found to exhibit a markedly truncated LOS of 2.9 kDa when compared by Tricine/SDS-PAGE to the parental LOS (4. Read More

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