37,474 results match your criteria highly purified

Long-term safety and efficacy of add-on cannabidiol in patients with Lennox-Gastaut syndrome: Results of a long-term open-label extension trial.

Epilepsia 2021 Jul 20. Epub 2021 Jul 20.

New York University Comprehensive Epilepsy Center, New York, USA.

Objective: Lennox-Gastaut syndrome (LGS) is an epileptic encephalopathy that is often treatment resistant. Efficacy and safety of add-on cannabidiol (CBD) to treat seizures associated with LGS was demonstrated in two randomized controlled trials (RCTs). Patients who completed the RCTs were invited to enroll in this long-term open-label extension (OLE) trial, GWPCARE5 (NCT02224573). Read More

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Sensitive and Specific Immunohistochemistry Protocol for Nucleocapsid Protein from All Common SARS-CoV-2 Virus Strains in Formalin-Fixed, Paraffin Embedded Tissues.

Methods Protoc 2021 Jul 10;4(3). Epub 2021 Jul 10.

Department of Pathology, Medical College of Wisconsin, TBRC-C4980, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA.

Human coronavirus disease 2019 (COVID-19) is a life-threatening and highly contagious disease caused by coronavirus SARS-CoV-2. Sensitive and specific detection of SARS-CoV-2 viral proteins in tissues and cells of COVID-19 patients will support investigations of the biologic behavior and tissue and cell tropism of this virus. We identified commercially available affinity-purified polyclonal antibodies raised against nucleocapsid and spike proteins of SARS-CoV-2 that provide sensitive and specific detection of the virus by immunohistochemistry in formalin-fixed, paraffin-embedded tissue. Read More

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A scalable and reproducible preparation for the antitumor protein TLC, a human-derived telomerase inhibitor.

Protein Expr Purif 2021 Jul 17;187:105942. Epub 2021 Jul 17.

Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, 313000, China; School of Medicine, Huzhou University, Huzhou, 313000, China. Electronic address:

Telomerase, which is overexpressed in approximately 90% of liver cancer cells, is an ideal target for anti-liver cancer therapy. LPTS, a putative liver tumor suppressor, is the only human-derived protein that can bind telomerase directly and inhibit the extension of telomere activity. Our previous studies demonstrated that TAT-LPTS-LC (TLC), a recombinant protein fused by the C-terminal 133-328 fragment of LPTS and TAT peptides, could be delivered into cells to inhibit telomerase-positive hepatoma cell growth in vitro and in vivo with very low toxicity. Read More

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Development of double antibody sandwich ELISA as potential diagnostic tool for rapid detection of Crimean-Congo hemorrhagic fever virus.

Sci Rep 2021 Jul 19;11(1):14699. Epub 2021 Jul 19.

Division of Virology, Defence Research and Development Establishment, Gwalior, 474002, India.

Crimean-Congo hemorrhagic fever (CCHF) virus, a highly pathogenic viral agent is responsible for severe fatal hemorrhagic infections in many parts of the world. The early diagnosis of CCHF infection is important for successful clinical management and epidemiological control. The nucleoprotein (NP) of CCHFV being highly conserved and immunogenic is used as early diagnostic marker. Read More

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Biotechnological Insights on the Expression and Production of Antimicrobial Peptides in Plants.

Molecules 2021 Jul 1;26(13). Epub 2021 Jul 1.

Research Unit for Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok 10330, Thailand.

The emergence of drug-resistant pathogens poses a serious critical threat to global public health and requires immediate action. Antimicrobial peptides (AMPs) are a class of short peptides ubiquitously found in all living forms, including plants, insects, mammals, microorganisms and play a significant role in host innate immune system. These peptides are considered as promising candidates to treat microbial infections due to its distinct advantages over conventional antibiotics. Read More

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Longitudinal impact of cannabidiol on EEG measures in subjects with treatment-resistant epilepsy.

Epilepsy Behav 2021 Jul 14;122:108190. Epub 2021 Jul 14.

Department of Neurology and the UAB Epilepsy Center, University of Alabama at Birmingham, Birmingham, AL, USA.

Objective: To assess the longitudinal impact of highly purified cannabidiol (CBD) on the electroencephalogram (EEG) of children and adults.

Methods: Participants received an EEG prior to starting CBD, after approximately 12 weeks of CBD (FU1) and after approximately one year of CBD therapy (FU2). Longitudinal changes in five EEG measures (background frequency, focal slowing, reactivity, frequency of interictal, and ictal discharges) were examined following CBD exposure. Read More

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Fed-batch production assessment of a tetravalent bispecific antibody: A case study on piggyBac stably transfected HEK293 cells.

N Biotechnol 2021 Jul 15;65:9-19. Epub 2021 Jul 15.

Department of Pharmaceutical Biosciences, Science for Life Laboratory, Uppsala University, Uppsala, Sweden. Electronic address:

The transition from preclinical biological drug development into clinical trials requires an efficient upscaling process. In this context, bispecific antibody drugs are particularly challenging due to their propensity to form aggregates and generally produce low titers. Here, the upscaling process for a tetravalent bispecific antibody expressed by a piggyBac transposon-mediated stable HEK293 cell pool has been evaluated. Read More

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Transformation of Acellular Dermis Matrix with Dicalcium Phosphate into 3D Porous Scaffold for Bone Regeneration.

J Biomater Sci Polym Ed 2021 Jul 16:1-15. Epub 2021 Jul 16.

Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, ca0009, PR China.

Animal derived biomaterials have attracted much attentions in treating large size bone defect due to their excellent biocompatibility and potent bioactivities offered by the biomacromolecules and growth factors contained in these materials. Dermis-derived matrix (ADM) has been used as skin grafts and wound dressings for decades, however its application in bone tissue engineering has been largely limited as ADM possesses a dense structure which does not support bone tissue ingrowth. Recently we have successfully fabricated porous scaffold structure using a ADM with the aid of micronization technique. Read More

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IgM single antigen bead HLA-assay is affected by imlifidase through the cleavage of IgG but not IgM.

Transpl Immunol 2021 Jul 13;68:101436. Epub 2021 Jul 13.

Hansa Biopharma AB, Lund, Sweden. Electronic address:

Aim: The aim of this study was to investigate if human IgM is a cleavable substrate for imlifidase and to explain an observed effect in anti-HLA IgM single antigen bead (SAB) assays in sensitized patients.

Methods: Serum samples collected pre- and 24 h post-imlifidase administration from sensitized patients enrolled in a phase II trial were investigated for anti-HLA IgG and IgM using SAB assays, with and without in vitro IgG depletion using a CaptureSelect™ affinity matrix. In addition, pre-dose samples and purified human IgM samples were treated with imlifidase in vitro and evaluated by SDS-PAGE, Western blot (PE-conjugated anti-human IgM) and SAB (IgG, IgM) assays. Read More

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Time to onset of cannabidiol treatment effects in Dravet syndrome: Analysis from two randomized controlled trials.

Epilepsia 2021 Jul 15. Epub 2021 Jul 15.

Paediatric Neurosciences Research Group, Royal Hospital for Children & College of Medical, Veterinary, and Life Sciences, University of Glasgow, Glasgow, UK.

Objective: We conducted a post hoc analysis of two randomized controlled trials, GWPCARE1 (NCT02091375) and GWPCARE2 (NCT02224703), to estimate the time to onset of cannabidiol (CBD) treatment effects (seizure reduction and adverse events [AEs]) in patients with Dravet syndrome (DS).

Methods: Patients received either plant-derived highly purified CBD (Epidiolex in the United States; 100 mg/ml oral solution) 10 mg/kg/day (CBD10; GWPCARE2) or 20 mg/kg/day (CBD20; GWPCARE1&2), or matching placebo for 14 weeks. Treatment started at 2. Read More

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Profiling chromatin accessibility in formalin-fixed paraffin-embedded samples.

Genome Res 2021 Jul 14. Epub 2021 Jul 14.

Uppsala University

Archived formalin-fixed paraffin-embedded (FFPE) samples are the global standard format for preservation of the majority of biopsies in both basic research and translational cancer studies, and profiling chromatin accessibility in the archived FFPE tissues is fundamental to understanding gene regulation. Accurate mapping of chromatin accessibility from FFPE specimens is challenging because of the high degree of DNA damage. Here, we first showed that standard ATAC-seq can be applied to purified FFPE nuclei but yields lower library complexity and a smaller proportion of long DNA fragments. Read More

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Role of BRCA2 DNA-binding and C-terminal domain on its mobility and conformation in DNA repair.

Elife 2021 Jul 13;10. Epub 2021 Jul 13.

Department of Molecular Genetics, Department of Radiation Oncology, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, Netherlands.

BRCA2 is an essential protein in genome maintenance, homologous recombination and replication fork protection. Its function includes multiple interaction partners and requires timely localization to relevant sites in the nucleus. We investigated the importance of the highly conserved DNA binding domain (DBD) and C-terminal domain (CTD) of BRCA2. Read More

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Icosapent ethyl: safely reducing cardiovascular risk in adults with elevated triglycerides.

Expert Opin Drug Saf 2021 Jul 12. Epub 2021 Jul 12.

Brigham and Women's Hospital Heart & Vascular Center, Harvard Medical School, Boston, MA, USA.

Introduction: In patients at high cardiovascular risk, the rate of events remains elevated despite traditional, evidence-based lipid-lowering therapy. Residual hypertriglyceridemia is an important contributor to this risk. However, prior medications with triglyceride-lowering effects have not demonstrated an ability to reduce adverse clinical outcomes in the statin era. Read More

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A Novel Family of RNA-Binding Proteins Regulate Polysaccharide Metabolism in .

J Bacteriol 2021 Jul 12:JB0021721. Epub 2021 Jul 12.

Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.

Human gut microbiome composition is constantly changing, and diet is a major driver of these changes. Gut microbial species that persist in mammalian hosts for long periods of time must possess mechanisms for sensing and adapting to nutrient shifts to avoid being outcompeted. Global regulatory mechanisms mediated by RNA-binding proteins (RBPs) that govern responses to nutrient shifts have been characterized in Proteobacteria and Firmicutes but remain undiscovered in the Bacteroidetes. Read More

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Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against r48/45 Proteins Produced in and Chinese Hamster Ovary Systems.

Front Immunol 2021 24;12:634738. Epub 2021 Jun 24.

Immunology Department, Malaria Vaccine and Drug Development Center, Cali, Colombia.

P48/45 is a conserved gametocyte antigen involved in parasite fertilization. A recombinant P48/45 (48/45) protein expressed in () was highly antigenic and immunogenic in experimental animals and elicited specific transmission-blocking (TB) antibodies in a previous pilot study. Here, a similar 48/45 gene was expressed in Chinese Hamster Ovary (CHO) cells and we compared its immunoreactivity with the product. Read More

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Identification of M-NH2-NH2 Intermediate and Rate Determing Step for Nitrogen Reduction with Bioinspired Sulfur-bonded FeW Catalyst.

Angew Chem Int Ed Engl 2021 Jul 10. Epub 2021 Jul 10.

Guangzhou University, School of Environmental Science and Engineering, CHINA.

The multimetallic sulfur-framework catalytic site of biological nitrogenases allows the efficient conversion of dinitrogen (N 2 ) to ammonia (NH 3 ) under ambient conditions. Inspired by biological nitrogenases, a bimetallic sulfide material (FeWS x @FeWO 4 ) was synthesized as a highly efficient N 2 reduction (NRR) catalyst by sulfur substitution of the surface of FeWO 4 nanoparticles. Thus prepared FeWS x @FeWO 4 catalysts exhibit a relatively high NH 3 production rate of 30. Read More

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Elucidation of the microstructure of an immuno-stimulatory polysaccharide purified from Korean red ginseng using sequential hydrolysis.

Int J Biol Macromol 2021 Jul 6;186:13-22. Epub 2021 Jul 6.

Department of Food Science and Biotechnology, Kyonggi University, 154-42, Gwanggyosan-ro, Youngtong-gu, Suwon, Gyeonggi 16227, Republic of Korea. Electronic address:

The elucidation of the structural characteristics of polysaccharides from natural sources is generally difficult owing to their structural complexity and heterogeneity. In our previous study, an immuno-stimulatory polysaccharide (RGP-AP-I) was isolated from Korean red ginseng (Panax ginseng C.A. Read More

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A bivalent protein targeting glycans and HR1 domain in spike protein potently inhibited infection of SARS-CoV-2 and other human coronaviruses.

Cell Biosci 2021 Jul 8;11(1):128. Epub 2021 Jul 8.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences and Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, 200032, China.

Background: Our previous studies have shown that combining the antiviral lectin GRFT and the pan-CoV fusion inhibitory peptide EK1 results in highly potent inhibitory activity against SARS-CoV-2 infection. In this study, we aimed to design and construct a bivalent protein consisting of GRFT and EK1 components and evaluate its inhibitory activity and mechanism of action against infection by SARS-CoV-2 and its mutants, as well as other human coronaviruses (HCoVs).

Methods: The bivalent proteins were expressed in E. Read More

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Cell-free Biosynthesis of Chlorogenic Acid Using a Mixture of Chassis Cell Extracts and Purified Spy-Cyclized Enzymes.

J Agric Food Chem 2021 Jul 8;69(28):7938-7947. Epub 2021 Jul 8.

Institute of Synthetic Biology, MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.

A novel cell-free biosynthesis system based on a mixture of chassis cell extracts and purified Spy-cyclized enzymes (CFBS-mixture) was developed. As a demonstration, the CFBS-mixture was applied to chlorogenic acid (CGA) biosynthesis. The mix-and-match and Plackett-Burman experiments demonstrated that hydroxycinnamate-CoA quinate transferase and -hydroxyphenylacetate 3-hydroxylase were the key enzymes for the production of CGA. Read More

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Tandem Affinity Purification (TAP) of Low-Abundance Protein Complexes in Filamentous Fungi Demonstrated Using Magnaporthe oryzae.

Methods Mol Biol 2021 ;2356:97-108

Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA.

Protein-protein interactions underlie cellular structure and function. In recent years, a number of methods have been developed for the identification of protein complexes and component proteins involved in the control of various biological pathways. Tandem affinity purification (TAP) coupled with mass spectrometry (MS) is a powerful method enabling the isolation of high-purity native protein complexes under mild conditions by performing two sequential purification steps using two different epitope tags. Read More

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January 2021

Pharmacological Management of Diabetes Mellitus: A Century of Expert Opinions in Cecil Textbook of Medicine.

Am J Ther 2021 Jun 22;28(4):e397-e410. Epub 2021 Jun 22.

Department M3 Internal Medicine I "George Emil Palade" University of Medicine, Pharmacy, Science and Technology of Târgu Mure, Romania; and.

Background: Drug therapy for diabetes mellitus (DM) has had a significant impact on quality of life and work potential of affected persons and has contributed to a remarkable decrease in the frequency and severity of complications, hospitalizations, and mortality. The current approach is the result of incremental progress in using technological advances to increase the safety and effectiveness of insulin therapy and the introduction of new molecules as oral and injectable antidiabetic drugs.

Study Question: What are the milestones of the changes in the expert approach to the pharmacological management of DM in the past century?

Study Design: To determine the changes in the experts' approach to the management of DM, as presented in a widely used textbook in the United States. Read More

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[Simultaneous determination of three quaternary ammonium muscle relaxants in blood by high performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 Jul;39(7):695-701

State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.

Vecuronium, rocuronium, and pancuronium are widely used as non-depolarizing muscle relaxants. There have been occasional cases of allergic reactions and even death when using such muscle relaxants. Rapid determination of the concentration of these muscle relaxants in blood can provide valuable information for early clinical diagnosis. Read More

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[Applications of separation technology in novel coronavirus research, epidemic prevention and detection].

Se Pu 2021 Jul;39(7):679-685

School of Life Science, Beijing Institute of Technology, "Molecular Medicine and Biological Diagnosis" Key Laboratory of Ministry of Industry and Information Technology, Beijing 100081, China.

The novel coronavirus disease 2019 (COVID-19) outbreak has brought to light unprecedented challenges to global public health security. Researchers have devoted their efforts to in-depth research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to bring the epidemic under control as rapidly as possible. Among the many areas of burgeoning SARS-CoV-2 related research, various analytical technologies have been applied to the advancement of virus detection, and development of vaccines and innovative therapies. Read More

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[Determination of 16 organophosphate esters in human blood by high performance liquid chromatography-tandem mass spectrometry combined with liquid-liquid extraction and solid phase extraction].

Se Pu 2021 Jan;39(1):69-76

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100083, China.

Measurement of organophosphate esters (OPEs) in human body fluids is important for understanding human internal exposure to OPEs and for assessing related health risks. Most of the current studies have focused on the determination of OPE metabolites in human urine, as OPEs are readily metabolized into their diester or hydroxylated forms in the human body. However, given the existence of one metabolite across multiple OPEs or multiple metabolites of one OPE, as well as the low metabolic rates of several OPEs in studies, the reliability of urinary OPE metabolites as biomarkers for specific OPEs is needs to be treated with caution. Read More

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January 2021

[Advances in technologies for large-scale enrichment and identification of ribonucleic acid-protein complexes].

Se Pu 2021 Feb;39(2):105-111

Beijing Institute of Lifeomics, Beijing Proteome Research Center, National Center for Protein Sciences(Beijing), State Key Laboratory of Proteomics, Beijing 102206, China.

Ribonucleic acid (RNA) rarely exists alone in the cell. RNAs interact with a variety of proteins and form RNA-protein complexes (RP-complexes) in every step of their life cycle, from transcription to degradation. These RP-complexes play key roles in regulating a variety of physiological processes. Read More

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February 2021

Characterization and identification of SFDC-1, a novel AmpC-type β-lactamase in Serratia fonticola.

Environ Microbiol 2021 Jul 5. Epub 2021 Jul 5.

The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, 325027, China.

The clinical and environmental infections caused by AmpC β-lactamases have been increasingly reported recently. In this study, we characterize the novel chromosome-encoded AmpC β-lactamase SFDC-1 identified in Serratia fonticola strain R28, which was isolated from a rabbit raised on a farm in southern China. SFDC-1 shared the highest amino acid identity of 79. Read More

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Differential binding of human and murine IgGs to catalytic and cell wall binding domains of Staphylococcus aureus peptidoglycan hydrolases.

Sci Rep 2021 Jul 5;11(1):13865. Epub 2021 Jul 5.

Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, HPC EB80, P.O. box 30001, 9700 RB, Groningen, the Netherlands.

Staphylococcus aureus is an opportunistic pathogen causing high morbidity and mortality. Since multi-drug resistant S. aureus lineages are nowadays omnipresent, alternative tools for preventive or therapeutic interventions, like immunotherapy, are urgently needed. Read More

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Streptococcus equi-derived extracellular vesicles as a vaccine candidate against Streptococcus equi infection.

Vet Microbiol 2021 Aug 25;259:109165. Epub 2021 Jun 25.

Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea; Bio-Analytical Science, University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address:

Streptococcus equi subspecies equi is a pathogenic bacterium that causes strangles, a highly contagious respiratory infection in horses and other equines. The limitations of current vaccines against S. equi infection warrants the development of an affordable, safe, and effective vaccine. Read More

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Economic evaluation of highly purified human menotropin or recombinant follicle-stimulating hormone for controlled ovarian stimulation in high-responder patients: analysis of the Menopur in Gonadotropin-releasing Hormone Antagonist Single Embryo Transfer-High Responder (MEGASET-HR) trial.

F S Rep 2020 Dec 10;1(3):257-263. Epub 2020 Nov 10.

Ferring Pharmaceuticals, Inc., Parsippany, New Jersey.

Objective: To determine the cost of achieving a live birth after first transfer using highly purified human menotropin (HP-hMG) or recombinant follicle-stimulating hormone (FSH) for controlled ovarian stimulation in predicted high-responder patients in the Menopur in Gonadotropin-releasing hormone Antagonist Single Embryo Transfer-High Responder (MEGASET-HR) trial.

Design: Cost minimization analysis of trial results.

Setting: Thirty-one fertility centers. Read More

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December 2020

The effects of ingestion of hydrogen-dissolved alkaline electrolyzed water on stool consistency and gut microbiota: a double-blind randomized trial.

Med Gas Res 2021 Oct-Dec;11(4):138-144

Graduate School Department of Interdisciplinary Research, University of Yamanashi, Kofu, Yamanashi, Japan.

A relationship between Bifidobacterium and defecation has previously been reported. Our hypothesis on the effectiveness of alkaline electrolyzed water (AEW) proposes that ingestion of AEW, considered possessing antioxidative properties, increases the number of Bifidobacteria and improves stool hardness and gastrointestinal symptoms. A double-blind, randomized study was conducted to evaluate the connection between stool consistency and change in gut microbiota composition induced by drinking hydrogen-dissolved AEW. Read More

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