21,214 results match your criteria highly enriched

Agricultural practices drive biological loads, seasonal patterns and potential pathogens in the aerobiome of a mixed-land-use dryland.

Sci Total Environ 2021 Jul 22;798:149239. Epub 2021 Jul 22.

The Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University, Tempe 85287-5001, AZ, USA; School of Life Sciences, Arizona State University, Tempe, AZ 85287-5001, USA. Electronic address:

Air carries a diverse load of particulate microscopic biological matter in suspension, either aerosolized or aggregated with dust particles, the aerobiome, which is dispersed by winds from sources to sinks. The aerobiome is known to contain microbes, including pathogens, as well as debris or small-sized propagules from plants and animals, but its variability and composition has not been studied comprehensibly. To gain a dynamic insight into the aerobiome existing over a mixed-use dryland setting, we conducted a biologically comprehensive, year-long survey of its composition and dynamics for particles less than 10 μm in diameter based on quantitative analyses of DNA content coupled to genomic sequencing. Read More

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Resveratrol Attenuates Diquat-Induced Oxidative Stress by Regulating Gut Microbiota and Metabolome Characteristics in Piglets.

Front Microbiol 2021 12;12:695155. Epub 2021 Jul 12.

Hainan Key Lab of Tropical Animal Reproduction and Breeding and Epidemic Disease Research, College of Animal Science and Technology, Hainan University, Haikou, China.

Previous studies have shown that dietary resveratrol (RES) reduces diarrhea and attenuates oxidative stress in piglets challenged with diquat. However, the effect of dietary resveratrol on the gut microbiota of these piglets, as well as the potential relationships between intestinal microflora and metabolites, remain unclear. Here, ribosomal DNA sequencing and metabolome analyses were performed to investigate the effect of RES on the gut microbiota and metabolome of diquat-challenged piglets. Read More

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Prognostic Roles of Phosphofructokinase Platelet in Clear Cell Renal Cell Carcinoma and Correlation with Immune Infiltration.

Int J Gen Med 2021 20;14:3645-3658. Epub 2021 Jul 20.

Department of Urology, the First Hospital of Jilin University, Changchun, 130021, Jilin, People's Republic of China.

Background: Abnormal expression of phosphofructokinase platelet (PFKP) has been reported in various cancer types. However, the role of PFKP in clear cell renal cell carcinoma (ccRCC) remains unclear.

Methods: In this study, the PFKP expression levels in various cancers were systemically described by integrating multiple kinds of publicly available databases. Read More

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Plasma extracellular vesicle delivery of miR-210-3p by targeting ATG7 to promote sepsis-induced acute lung injury by regulating autophagy and activating inflammation.

Exp Mol Med 2021 Jul 28. Epub 2021 Jul 28.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 430060, Wuhan, P.R. China.

Extracellular vesicles (EVs) can be used for intercellular communication by facilitating the transfer of miRNAs from one cell to a recipient cell. MicroRNA (miR)-210-3p is released into the blood during sepsis, inducing cytokine production and promoting leukocyte migration. Thus, the current study aimed to elucidate the role of plasma EVs in delivering miR-210-3p in sepsis-induced acute lung injury (ALI). Read More

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Endogenous retroviruses drive lineage-specific regulatory evolution across primate and rodent placentae.

Mol Biol Evol 2021 Jul 28. Epub 2021 Jul 28.

The Eunice Kennedy Shriver National Institutes of Child Health and Human Development, NIH, Bethesda, MD, 20892, USA.

In mammals, the placenta mediates maternal-fetal nutrient and waste exchange and acts in an immunomodulatory way to facilitate maternal-fetal tolerance. The placenta is highly diverse across mammalian species, yet the molecular mechanisms that distinguish the placenta of human from other mammals are not fully understood. Using an interspecies transcriptomic comparison of human, macaque, and mouse late-gestation placentae, we identified hundreds of genes with lineage-specific expression-including dozens that are placentally-enriched and potentially related to pregnancy. Read More

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Decrypting the role of predicted SARS-CoV-2 miRNAs in COVID-19 pathogenesis: A bioinformatics approach.

Comput Biol Med 2021 Jul 21;136:104669. Epub 2021 Jul 21.

School of Medical Science and Technology, IIT Kharagpur, Kharagpur, Paschim Medinipur, West Bengal, 721302, India. Electronic address:

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is a highly transmissible virus causing the ongoing global pandemic, COVID-19. Evidence suggests that viral and host microRNAs play pivotal roles in progression of such infections. The decisive impact of viral miRNAs and their putative targets in modulating the transcriptomic profile of its host, however remains unexplored. Read More

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Inertial focusing of circulating tumor cells in whole blood at high flow rates using the microfluidic CTCKey™ device for CTC enrichment.

Lab Chip 2021 Jul 28. Epub 2021 Jul 28.

Chemical Engineering, University of Michigan, 2800 Plymouth Rd., Ann Arbor, Michigan, USA. and Department of Internal Medicine, University of Michigan Rogel Cancer Center, Ann Arbor, Michigan, USA.

Circulating tumor cells (CTCs) are extremely rare cells shed from tumors into the blood stream. These cells can provide valuable information about their tumor of origin and direct treatment decisions to improve patient outcomes. Current technologies isolate CTCs from a limited blood volume and often require pre-processing that leads to CTC loss, making it difficult to isolate enough CTCs to perform in-depth tumor analysis. Read More

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A Novel Human Long Noncoding RNA SCDAL Promotes Angiogenesis through SNF5-Mediated GDF6 Expression.

Adv Sci (Weinh) 2021 Jul 28:e2004629. Epub 2021 Jul 28.

Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310009, P. R. China.

Angiogenesis is essential for vascular development. The roles of regulatory long noncoding RNAs (lncRNAs) in mediating angiogenesis remain under-explored. Human embryonic stem cell-derived mesenchymal stem cells (hES-MSCs) are shown to exert more potent cardioprotective effects against cardiac ischemia than human bone marrow-derived MSCs (hBM-MSCs), associated with enhanced neovascularization. Read More

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Nanoscale Advancement Continues-From Catalysts and Reagents to Restructuring of Reaction Media.

Angew Chem Int Ed Engl 2021 Jul 28. Epub 2021 Jul 28.

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, 119991, Russia.

Comprehensive studies dedicated to the search for specific properties of matter at the micro- and nanoscales have greatly enriched the fields of chemistry and materials science. From the point of view of synthetic chemistry, discoveries in the field of nanoscale catalysis, in which the size effects of active centers are used to accelerate the reactions, are of particular importance. However, another approach for the promotion of chemical transformations based on the micro- or nanoconfinement of reacting molecules or even on the structuring of the reaction media as a whole is gaining interest as a highly valuable tool. Read More

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Identification of structural variation and polymorphisms of a sex co-segregating scaffold in spinach.

Plant Reprod 2021 Jul 28. Epub 2021 Jul 28.

Department of Plant Biology, University of Illinois At Urbana-Champaign, 1201 West Gregory Drive, Urbana, IL, 61801-3838, USA.

Spinach is a common vegetable, and dioecy is maintained by a pair of XY sex chromosomes. Due to limited genomic resources and its highly repetitive genome, limited studies were conducted to investigate the genomic landscape of the region near sex-determining loci. In this study, we screened the structure variations (SVs) between Y-linked contigs and a 1. Read More

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Analysis of pancreatic extracellular matrix protein post-translational modifications electrostatic repulsion-hydrophilic interaction chromatography coupled with mass spectrometry.

Mol Omics 2021 Jul 27. Epub 2021 Jul 27.

Department of Chemistry, University of Wisconsin-Madison, 777 Highland Ave., Madison, WI 53706, USA. and School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA.

The pancreas is a vital organ with digestive and endocrine roles, and diseases of the pancreas affect millions of people yearly. A better understanding of the pancreas proteome and its dynamic post-translational modifications (PTMs) is necessary to engineer higher fidelity tissue analogues for use in transplantation. The extracellular matrix (ECM) has major roles in binding and signaling essential to the viability of insulin-producing islets of Langerhans. Read More

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Analysis of long intergenic non-coding RNAs transcriptomic profiling in skeletal muscle growth during porcine embryonic development.

Sci Rep 2021 Jul 27;11(1):15240. Epub 2021 Jul 27.

Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China.

Skeletal muscle growth plays a critical role during porcine muscle development stages. Genome-wide transcriptome analysis reveals that long intergenic non-coding RNAs (lincRNAs) are implicated as crucial regulator involving in epigenetic regulation. However, comprehensive analysis of lincRNAs in embryonic muscle development stages remain still elusive. Read More

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Dynamic changes of miRNAs in skeletal muscle development at New Zealand rabbits.

BMC Genomics 2021 Jul 27;22(1):577. Epub 2021 Jul 27.

College of Animal Science and Technology, Anhui Agricultural University, Anhui, 230036, Hefei, People's Republic of China.

Background: miRNA is one of the crucial roles in the complex and dynamic network that regulates the development of skeletal muscle. The landscape of skeletal muscle miRNAs from fetus to adult in New Zealand rabbits has not been revealed yet.

Results: In this study, nine RNA-seq libraries of fetus, child and adult rabbits' leg muscles were constructed. Read More

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Chitosan-Calcium-Simvastatin Scaffold as an Inductive Cell-Free Platform.

J Dent Res 2021 Jul 27:220345211024207. Epub 2021 Jul 27.

Department of Physiology and Pathology, University of Estadual Paulista-UNESP, Araraquara School of Dentistry, Araraquara, SP, Brazil.

The development of biomaterials based on the combination of biopolymers with bioactive compounds to develop delivery systems capable of modulating dentin regeneration mediated by resident cells is the goal of current biology-based strategies for regenerative dentistry. In this article, the bioactive potential of a simvastatin (SV)-releasing chitosan-calcium-hydroxide (CH-Ca) scaffold was assessed. After the incorporation of SV into CH-Ca, characterization of the scaffold was performed. Read More

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Integration of selection signatures and multi-trait GWAS reveals polygenic genetic architecture of carcass traits in beef cattle.

Genomics 2021 Jul 24. Epub 2021 Jul 24.

Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:

Carcass quality is significantly important in beef industry and has been recognized as a hot research topic. However, the genetic basis of carcass traits remains to be well understood. Here, we applied multiple methods, including the Composite of Likelihood Ratio (CLR) and Genome-wide Association Study (GWAS), to explore the selection signatures and candidate variants affecting carcass traits. Read More

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An aptamer highly specific to cellulose enables the analysis of the association of cellulose with matrix cell wall polymers in vitro and in muro.

Plant J 2021 Jul 27. Epub 2021 Jul 27.

Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, DK-1871, Denmark.

The current toolbox of cell wall-directed molecular probes has been pivotal for advancing basic and application-oriented plant carbohydrate research; however, it still exhibits limitations regarding target diversity and specificity. Scarcity of probes targeting intramolecular associations between cell wall polymers especially hinders our understanding of the cell wall microstructure and affects the development of effective means for its efficient deconstruction for bioconversion. Here we report a detailed characterization of a cellulose-binding DNA aptamer CELAPT MINI using a combination of various in vitro biochemical, biophysical, and molecular biology techniques. Read More

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Curr Probl Cardiol 2021 Jul 23:100943. Epub 2021 Jul 23.

Head of Molecular Biology Department, Kuban State Medical University, Krasnodar, Russian Federation.

Introduction: Today, cardiovascular diseases, due to their widespread prevalence, are among the most relevant biomedical problems in the modern world. The development of cardiovascular comorbidity among patients with diabetes mellitus is of high clinical urgency. Therefore, the study of cardiovascular risk modification among patients with diabetes mellitus is of paramount importance. Read More

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Active Sites and Interfacial Reducibility of CuO/CeO Catalysts Induced by Reducing Media and O/H Activation.

ACS Appl Mater Interfaces 2021 Jul 27. Epub 2021 Jul 27.

Inner Mongolia Key Laboratory of Chemistry and Physics of Rare Earth Materials, School of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, P. R. China.

The development of a highly efficient and stable catalyst for preferential oxidation of CO for the commercialization of proton-exchange membrane fuel cells has been a result of continuous effort. The main challenge is the simultaneous control of abundant active sites and interfacial reducibility over the catalyst CuO/CeO. Here, we report a strategy to modulate porosity, active sites, and O-vacancy sites (O) by reducing media and O/H activation. Read More

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Recent ultra-rare inherited variants implicate new autism candidate risk genes.

Nat Genet 2021 Jul 26. Epub 2021 Jul 26.

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Autism is a highly heritable complex disorder in which de novo mutation (DNM) variation contributes significantly to risk. Using whole-genome sequencing data from 3,474 families, we investigate another source of large-effect risk variation, ultra-rare variants. We report and replicate a transmission disequilibrium of private, likely gene-disruptive (LGD) variants in probands but find that 95% of this burden resides outside of known DNM-enriched genes. Read More

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Mitochondrial damage in renal epithelial cells is potentiated by protein exposure in propionic aciduria.

J Inherit Metab Dis 2021 Jul 23. Epub 2021 Jul 23.

Department of General Paediatrics, Adolescent Medicine and Neonatology, Medical Center-University of Freiburg, Faculty of Medicine, Freiburg, Germany.

Propionic aciduria (PA) is caused by deficiency of the mitochondrial enzyme propionyl-CoA carboxylase (PCC). Due to inefficient propionate catabolism patients are endangered by life-threatening ketoacidotic crisis. Protein and amino acid restriction are major therapeutic pillars. Read More

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A data-driven performance dashboard for surgical dissection.

Sci Rep 2021 Jul 22;11(1):15013. Epub 2021 Jul 22.

Project neuroArm, Department of Clinical Neurosciences, and Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.

Surgical error and resulting complication have significant patient and economic consequences. Inappropriate exertion of tool-tissue force is a common variable for such error, that can be objectively monitored by sensorized tools. The rich digital output establishes a powerful skill assessment and sharing platform for surgical performance and training. Read More

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Conserved immunomodulatory transcriptional networks underlie antipsychotic-induced weight gain.

Transl Psychiatry 2021 Jul 22;11(1):405. Epub 2021 Jul 22.

Division of Endocrinology and Metabolism, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.

Although antipsychotics, such as olanzapine, are effective in the management of psychiatric conditions, some patients experience excessive antipsychotic-induced weight gain (AIWG). To illuminate pathways underlying AIWG, we compared baseline blood gene expression profiles in two cohorts of mice that were either prone (AIWG-P) or resistant (AIWG-R) to weight gain in response to olanzapine treatment for two weeks. We found that transcripts elevated in AIWG-P mice relative to AIWG-R are enriched for high-confidence transcriptional targets of numerous inflammatory and immunomodulatory signaling nodes. Read More

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Transcriptional-regulatory convergence across functional MDD risk variants identified by massively parallel reporter assays.

Transl Psychiatry 2021 Jul 22;11(1):403. Epub 2021 Jul 22.

Departments of Genetics and Psychiatry, Washington University in St. Louis, St. Louis, MO, USA.

Family and population studies indicate clear heritability of major depressive disorder (MDD), though its underlying biology remains unclear. The majority of single-nucleotide polymorphism (SNP) linkage blocks associated with MDD by genome-wide association studies (GWASes) are believed to alter transcriptional regulators (e.g. Read More

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Genome-wide DNA methylome analysis identifies methylation signatures associated with survival and drug resistance of ovarian cancers.

Clin Epigenetics 2021 Jul 22;13(1):142. Epub 2021 Jul 22.

Department of Obstetrics and Gynaecology, L747 Laboratory Block, LKS Faculty of Medicine, 21 Sassoon Road, Pokfulam, Hong Kong, SAR, People's Republic of China.

Background: In contrast to stable genetic events, epigenetic changes are highly plastic and play crucial roles in tumor evolution and development. Epithelial ovarian cancer (EOC) is a highly heterogeneous disease that is generally associated with poor prognosis and treatment failure. Profiling epigenome-wide DNA methylation status is therefore essential to better characterize the impact of epigenetic alterations on the heterogeneity of EOC. Read More

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Breast cancer cell-derived extracellular vesicles transfer miR-182-5p and promote breast carcinogenesis via the CMTM7/EGFR/AKT axis.

Mol Med 2021 Jul 16;27(1):78. Epub 2021 Jul 16.

Department of breast and thyroid surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, Hubei, China.

Background: Extracellular vesicles (EVs) derived from tumor cells are implicated in the progression of malignancies through the transfer of molecular cargo microRNAs (miRNAs or miRs). We aimed to explore the role of EVs derived from breast cancer cells carrying miR-182-5p in the occurrence and development of breast cancer.

Methods: Differentially expressed miRNAs and their downstream target genes related to breast cancer were screened through GEO and TCGA databases. Read More

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Large-scale analysis reveals spatiotemporal circadian patterns of cilia transcriptomes in the primate brain.

J Neurosci Res 2021 Jul 26. Epub 2021 Jul 26.

Institute for Genomics and Bioinformatics, School of Information and Computer Sciences, University of California-Irvine, Irvine, CA, USA.

Cilia are dynamic subcellular systems, with core structural and functional components operating in a highly coordinated manner. Since many environmental stimuli sensed by cilia are circadian in nature, it is reasonable to speculate that genes encoding cilia structural and functional components follow rhythmic circadian patterns of expression. Using computational methods and the largest spatiotemporal gene expression atlas of primates, we identified and analyzed the circadian rhythmic expression of cilia genes across 22 primate brain areas. Read More

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Edge-Rich Bicrystalline 1T/2H-MoS Cocatalyst-Decorated {110} Terminated CeO Nanorods for Photocatalytic Hydrogen Evolution.

ACS Appl Mater Interfaces 2021 Jul 26. Epub 2021 Jul 26.

School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, South Australia 5005, Australia.

Developing all-solid-state Z-scheme systems with highly active photocatalysts are of huge interest in realizing long-term solar-to-fuel conversion. Here we reported an innovative hybrid of {110}-oriented CeO nanorods with edge-enriched bicrystalline 1T/2H-MoS coupling as efficient photocatalysts for water splitting. In the composites, the metallic 1T phase acts as an excellent solid state electron mediator in the Z-scheme, while the 2H phase and CeO are the adsorption sites of the photosensitizer and reactant (HO), respectively. Read More

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Alterations, Interactions, and Diagnostic Potential of Gut Bacteria and Viruses in Colorectal Cancer.

Front Cell Infect Microbiol 2021 6;11:657867. Epub 2021 Jul 6.

Institute for Intestinal Diseases, Tongji University School of Medicine, Shanghai, China.

Gut microbiome alteration was closely associated with colorectal cancer (CRC). Previous studies had demonstrated the bacteria composition changes but lacked virome profiles, trans-kindom interactions, and reliable diagnostic model explorations in CRC. Hence, we performed metagenomic sequencing to investigate the gut microbiome and microbial interactions in adenoma and CRC patients. Read More

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Molecular gut content analysis indicates the inter- and intra-guild predation patterns of spiders in conventionally managed vegetable fields.

Ecol Evol 2021 Jul 27;11(14):9543-9552. Epub 2021 Jun 27.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University Fuzhou China.

Inter- and intra-guild interactions are important in the coexistence of predators and their prey, especially in highly disturbed vegetable cropping systems with sporadic food resources. Assessing the dietary range of a predator taxon characterized by diverse foraging behavior using conventional approaches, such as visual observation and conventional molecular approaches for prey detection, has serious logistical problems. In this study, we assessed the prey compositions and compare the dietary spectrum of a functionally diverge group of predators-spiders-to characterize their trophic interactions and assess biological control potential in Brassica vegetable fields. Read More

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Seasonal Variation in Transcriptomic Profiling of Fully Developed Tuberous Roots Enriches Candidate Genes in Essential Metabolic Pathways and Phytohormone Signaling.

Front Plant Sci 2021 9;12:659645. Epub 2021 Jul 9.

Department of Biotechnology, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Diels et Gilg (Sanyeqing, SYQ) is a perennial climbing liana and an endemic plant to southern China. Its tuberous roots (TRs) are used in traditional Chinese medicine for treating some diseases such as high fever, pneumonia, asthma, hepatitis, and cancers. However, the mechanisms underlying the development of TR and the content of flavonoids and phenylpropanoids (FPs) are not well-understood. Read More

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