58,023 results match your criteria highly critical


Steady-State Pre-rRNA Analysis to Investigate the Functional Microbiome.

Curr Protoc 2021 Jul;1(7):e209

Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington.

The gut microbiome is recognized as a critical regulator of human diseases. Constituents of the microbiota and their individual activities can affect a broad range of disease states related to autoimmunity, cancer, infection, metabolism, mental health, and toxicant exposure. A substantial number of microbiome species are not culturable, limiting their study in vitro. Read More

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A new bottom-up method for the standard analysis and comparison of workforce capacity in mental healthcare planning: Demonstration study in the Australian Capital Territory.

PLoS One 2021 27;16(7):e0255350. Epub 2021 Jul 27.

Centre for Mental Health Research, Australian National University, Canberra, Australian Capital Territory, Australia.

The aims of this study are to evaluate and describe mental health workforce and capacity, and to describe the relationship between workforce capacity and patterns of care in local areas. We conducted a comparative demonstration study of the applicability of an internationally validated standardised service classification instrument-the Description and Evaluation of Services and Directories-DESDE-LTC) using the emerging mental health ecosystems research (MHESR) approach. Using DESDE-LTC as the framework, and drawing from international occupation classifications, the workforce was classified according to characteristics including the type of care provided and professional background. Read More

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Automated Phylogenetic Analysis Using Best Reciprocal BLAST.

Methods Mol Biol 2021 ;2369:41-63

Wellcome Centre for Anti-infectives Research, School of Life Sciences, University of Dundee, Dundee, UK.

Reconstruction of the evolutionary history of specific protein-coding genes is an essential component of the biological sciences toolkit and relies on identification of orthologs (a gene in different organisms related by vertical descent from a common ancestor and usually presumed to have the same or similar function) and paralogs (a gene related to another in the same organism by descent from a single ancestral gene which may, or may not, retain the same/similar function) across a range of taxa. While obviously essential for the reconstruction of evolutionary histories, ortholog identification is of importance for protein expression, modeling for drug discovery programs, identification of critical residues and other studies. Here we describe an automated system for searching for orthologs and paralogs in eukaryotic organisms. Read More

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January 2021

Green supplier selection for textile industry: a case study using BWM-TODIM integration under interval type-2 fuzzy sets.

Environ Sci Pollut Res Int 2021 Jul 27. Epub 2021 Jul 27.

Department of Emergency Aid and Disaster Management, Munzur University, 62000, Tunceli, Turkey.

Although environmental awareness has reached a high level, enterprises-regardless of their working domains-follow the concept of greenness for their practices. This awareness among the stakeholders and supply chain experts has a positive impact on the purchasing departments of enterprises in various sectors to consider greenness in their procurement processes. The critical decision that must be made in green supply chain management (GSCM) is supplier selection. Read More

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LINC00667 Promotes Progression of Esophageal Cancer Cells by Regulating miR-200b-3p/SLC2A3 Axis.

Dig Dis Sci 2021 Jul 27. Epub 2021 Jul 27.

Department of Gastroenterology, Taishan Hospital, Taian, Shandong Province, China.

Background: Recently, more and more evidence indicated that the long non-coding RNA was strictly related to the occurrence and progression of human cancers, including esophageal cancer (EC). We observed that LINC00667 was increased in EC, but the function of LINC00667 was unclear. Therefore, the function and potential molecular mechanism of LINC00667 in the progression of EC need to be further studied. Read More

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TSMiner: a novel framework for generating time-specific gene regulatory networks from time-series expression profiles.

Nucleic Acids Res 2021 Jul 27. Epub 2021 Jul 27.

State Key Laboratory of Proteomics, Beijing Institute of Lifeomics, National Center for Protein Sciences (Beijing), Beijing 102206, P.R. China.

Time-series gene expression profiles are the primary source of information on complicated biological processes; however, capturing dynamic regulatory events from such data is challenging. Herein, we present a novel analytic tool, time-series miner (TSMiner), that can construct time-specific regulatory networks from time-series expression profiles using two groups of genes: (i) genes encoding transcription factors (TFs) that are activated or repressed at a specific time and (ii) genes associated with biological pathways showing significant mutual interactions with these TFs. Compared with existing methods, TSMiner demonstrated superior sensitivity and accuracy. Read More

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Highly Efficient Activation of HCl Dissociation on Au(111) via Rotational Preexcitation.

J Phys Chem Lett 2021 Jul 27:7252-7260. Epub 2021 Jul 27.

Gorlaeus Laboratories, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.

The probability for dissociation of molecules on metal surfaces, which often controls the rate of industrially important catalytic processes, can depend strongly on how energy is partitioned in the incident molecule. There are many example systems where the addition of vibrational energy promotes reaction more effectively than the addition of translational energy, but for rotational pre-excitation similar examples have not yet been discovered. Here, we make an experimentally testable theoretical prediction that adding energy to the rotation of HCl can promote its dissociation on Au(111) 20 times more effectively than increasing its translational energy. Read More

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Endogenous Hemoprotein-Dependent Signaling Pathways of Nitric Oxide and Nitrite.

Inorg Chem 2021 Jul 27. Epub 2021 Jul 27.

Heart, Lung, Blood, and Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.

Interdisciplinary research at the interface of chemistry, physiology, and biomedicine have uncovered pivotal roles of nitric oxide (NO) as a signaling molecule that regulates vascular tone, platelet aggregation, and other pathways relevant to human health and disease. Heme is central to physiological NO signaling, serving as the active site for canonical NO biosynthesis in nitric oxide synthase (NOS) enzymes and as the highly selective NO binding site in the soluble guanylyl cyclase receptor. Outside of the primary NOS-dependent biosynthetic pathway, other hemoproteins, including hemoglobin and myoglobin, generate NO via the reduction of nitrite. Read More

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Au···H-C Hydrogen Bonds as Design Principle in Gold(I) Catalysis.

Angew Chem Int Ed Engl 2021 Jul 27. Epub 2021 Jul 27.

Ruhr-Universitat Bochum, Fakultät für Chemie und Biochemie, Universitätsstraße 150, 44780, Bochum, GERMANY.

Secondary ligand metal interactions are decisive in many catalytic transformations. While arene-gold interactions have repeatedly been reported as critical structural feature in many high-performance gold catalysts, we herein report that these interactions can also be replaced by Au···H-C hydrogen bonds without suffering any reduction in catalytic performance. Systematic experimental and computational studies on a series of ylide-substituted phosphines featuring either a PPh 3 ( Ph YPhos) or PCy 3 ( Cy YPhos) moiety showed that the arene-gold interaction in the aryl-substituted compounds is efficiently compensated by the formation of Au···H-C hydrogen bonds. Read More

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Coupling of dynamic microtubules to F-actin by Fmn2 regulates chemotaxis of neuronal growth cones.

J Cell Sci 2021 07 8;134(13). Epub 2021 Jul 8.

Indian Institute of Science Education and Research (IISER) Pune, Dr Homi Bhabha Road, Pune 411008, India.

Dynamic co-regulation of the actin and microtubule subsystems enables the highly precise and adaptive remodelling of the cytoskeleton necessary for critical cellular processes, such as axonal pathfinding. The modes and mediators of this interpolymer crosstalk, however, are inadequately understood. We identify Fmn2, a non-diaphanous-related formin associated with cognitive disabilities, as a novel regulator of cooperative actin-microtubule remodelling in growth cones of both chick and zebrafish neurons. Read More

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Building resilience for healthcare professionals working in an Italian Red Zone during the COVID-19 outbreak: a pilot study.

Stress Health 2021 Jul 26. Epub 2021 Jul 26.

Canada Research Chair in Child, Family and Community Resilience, Resilience Research Centre Dalhousie University, PO Box, 15000, Halifax, NS, Canada.

The COVID-19 pandemic has placed considerable strain on healthcare workers showing high rates of stress and psychological health problems. Interventions are urgently needed to help healthcare workers perform under conditions of great risk and uncertainty. In particular, healthcare leadership is known to be critical to supporting healthcare workers to deal with an uncertain and distressing healthcare environment. Read More

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Highly sensitive time-resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco.

J Appl Microbiol 2021 Jul 27. Epub 2021 Jul 27.

China National Tobacco Quality Supervision and Test Center, Zhengzhou, China.

Aims: Alternaria longipes is a causal agent of brown spot of tobacco, which remains a serious threat to tobacco production. Herein, we established a detection method for A. longipes in tobacco samples based on the principle of time-resolved fluoroimmunoassay, in order to fulfil the requirement of rapid, sensitive and accurate detection in situ. Read More

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DSCR1 upregulation enhances dural meningeal lymphatic drainage to attenuate amyloid pathology of Alzheimer's disease.

J Pathol 2021 Jul 26. Epub 2021 Jul 26.

Department of Biological Sciences, College of Information and Biotechnology, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea.

Highly developed meningeal lymphatics remove waste products from the brain. Disruption of meningeal lymphatic vessels in a mouse model of amyloid pathology (5XFAD) accelerates the accumulation of amyloid plaques in the meninges and brain, and causes learning and memory deficits, suggesting that clearance of toxic wastes by lymphatic vessels plays a key role in neurodegenerative diseases. Here, we discovered that DSCR1 (Down syndrome critical region 1 known also as RCAN1, regulator of calcineurin 1) facilitates the drainage of waste products by increasing the coverage of dorsal meningeal lymphatic vessels. Read More

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Peak radial growth of diffuse-porous species occurs during periods of lower water availability than for ring-porous and coniferous trees.

Tree Physiol 2021 Jul 26. Epub 2021 Jul 26.

Harvard Forest, Harvard University, Petersham, MA, 10366, USA.

Climate models project warmer summer temperatures will increase the frequency and heat severity of droughts in temperate forests of Eastern North America. Hotter droughts are increasingly documented to affect tree growth and forest dynamics, with critical impacts on tree mortality, carbon sequestration, and timber provision. The growing acknowledgement of the dominant role of drought timing on tree vulnerability to water deficit raises the issue of our limited understanding of radial growth phenology for most temperate tree species. Read More

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Cross neutralization of emerging SARS-CoV-2 variants of concern by antibodies targeting distinct epitopes on spike.

Res Sq 2021 Jul 19. Epub 2021 Jul 19.

Several severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants have arisen that exhibit increased viral transmissibility and partial evasion of immunity induced by natural infection and vaccination. To address the specific antibody targets that were affected by recent viral variants, we generated 43 monoclonal antibodies (mAbs) from 10 convalescent donors that bound three distinct domains of the SARS-CoV-2 spike. Viral variants harboring mutations at K417, E484 and N501 could escape most of the highly potent antibodies against the receptor binding domain (RBD). Read More

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SENP1 promotes MCL pathogenesis through regulating JAK-STAT5 pathway and SOCS2 expression.

Cell Death Discov 2021 Jul 26;7(1):192. Epub 2021 Jul 26.

Department of Hematology, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

Mantle cell lymphoma (MCL) is highly aggressive and its treatment remains challenging, understanding its pathogenesis is critical for future targeted therapy. SUMO specific proteases 1 (SENP1) is an important protein that regulates the balance between SUMOylation and deSUMOylation. We found that SENP1 was upregulated in MCL patient samples and cell lines. Read More

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The Quality of SARS-CoV-2-Specific T Cell Functions Differs in Patients with Mild/Moderate versus Severe Disease, and T Cells Expressing Coinhibitory Receptors Are Highly Activated.

J Immunol 2021 Jul 26. Epub 2021 Jul 26.

School of Dentistry, Division of Foundational Sciences, University of Alberta, Edmonton, Alberta, Canada;

Understanding the function of SARS-CoV-2 Ag-specific T cells is crucial for the monitoring of antiviral immunity and vaccine design. Currently, both impaired and robust T cell immunity is described in COVID-19 patients. In this study, we explored and compared the effector functions of SARS-CoV-2-reactive T cells expressing coinhibitory receptors and examine the immunogenicity of SARS-CoV-2 S, M, and N peptide pools in regard to specific effector T cell responses, Th1/Th2/Th17, in COVID-19 patients. Read More

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Cyclic nucleotide phosphodiesterase 1C contributes to abdominal aortic aneurysm.

Proc Natl Acad Sci U S A 2021 Aug;118(31)

Aab Cardiovascular Research Institute, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642;

Abdominal aortic aneurysm (AAA) is characterized by aorta dilation due to wall degeneration, which mostly occurs in elderly males. Vascular aging is implicated in degenerative vascular pathologies, including AAA. Cyclic nucleotide phosphodiesterases, by hydrolyzing cyclic nucleotides, play critical roles in regulating vascular structure remodeling and function. Read More

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VMP1, a novel prognostic biomarker, contributes to glioma development by regulating autophagy.

J Neuroinflammation 2021 Jul 26;18(1):165. Epub 2021 Jul 26.

Shanghai Key Laboratory of Radiation Oncology (20dz2261000), Shanghai, 201321, China.

Background: Malignant glioma, especially glioblastoma, is a highly aggressive disease with a dismal prognosis. Vacuole membrane protein 1 (VMP1) is a critical autophagy-associated protein with roles in oncogenesis and tumor progression. However, the contribution of VMP1 to glioma development as well as its prognostic value has not been established. Read More

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Pathophysiology of Brain Injury and Neurological Outcome in Acute Respiratory Distress Syndrome: A Scoping Review of Preclinical to Clinical Studies.

Neurocrit Care 2021 Jul 23. Epub 2021 Jul 23.

Division of Neuroscience Critical Care, Departments of Neurology, Neurosurgery, Anesthesiology and Critical Care Medicine, Johns Hopkins University, 600 N. Wolfe Street, Phipps 455, Baltimore, MD, USA.

Acute respiratory distress syndrome (ARDS) has been associated with secondary acute brain injury (ABI). However, there is sparse literature on the mechanism of lung-mediated brain injury and prevalence of ARDS-associated secondary ABI. We aimed to review and elucidate potential mechanisms of ARDS-mediated ABI from preclinical models and assess the prevalence of ABI and neurological outcome in ARDS with clinical studies. Read More

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Depression of Pyroptosis by Inhibiting Caspase-1 Activation Improves Neurological Outcomes of Kernicterus Model Rats.

ACS Chem Neurosci 2021 Jul 23. Epub 2021 Jul 23.

Department of Neonatology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China.

Kernicterus is a severe complication of extreme neonatal hyperbilirubinemia. Prolonged exposure to high-level unconjugated bilirubin (UCB) directly damages brain tissue. Neuroinflammation is believed to contribute to UCB-induced neurotoxicity. Read More

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LungBEAM: A prospective multicenter study to monitor stage IV NSCLC patients with EGFR mutations using BEAMing technology.

Cancer Med 2021 Jul 23. Epub 2021 Jul 23.

Medical Oncology Department, Hospital Universitario Vall d'Hebron, Barcelona, Spain.

Objectives: The aim of LungBEAM was to determine the value of a novel epidermal growth factor receptor (EGFR) mutation test in blood based on BEAMing technology to predict disease progression in advanced non-small cell lung cancer (NSCLC) patients treated with first- or second-generation EGFR-tyrosine kinase inhibitors (EGFR-TKIs). Another goal was to monitor the dynamics of EGFR mutations, as well as to track EGFR exon 20 p.T790M (p. Read More

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CircTMC5 promotes gastric cancer progression and metastasis by targeting miR-361-3p/RABL6.

Gastric Cancer 2021 Jul 22. Epub 2021 Jul 22.

Department of General Surgery, The Fourth Affiliated Hospital of Anhui Medical University, No. 100 Huaihai Avenue, Xinzhan District, Hefei City, 230000, Anhui Province, China.

Background: Gastric cancer (GC) is common in East Asia, yet its molecular and pathogenic mechanisms remain unclear. Circular RNAs (circRNAs) are differentially expressed in GC and may be promising biomarkers. Here, we investigated the role and regulatory mechanism of circTMC5 in GC. Read More

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BCG therapy is associated with long-term, durable induction of Treg signature genes by epigenetic modulation.

Sci Rep 2021 Jul 22;11(1):14933. Epub 2021 Jul 22.

Immunobiology Laboratories, Massachusetts General Hospital, Bldg 149, 13th Street, Boston, MA, 02116, USA.

Induction of immunosuppressive T-regulatory cells (Tregs) is a desirable goal in autoimmunity, and perhaps other immune diseases of activation. One promising avenue is with the bacille-calmette-guérin (BCG) vaccine in autoimmune type 1 diabetes (T1D). Its administration is associated with gradual clinical improvements in human autoimmunity over a 2-3 year post-vaccination period. Read More

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Structural characterization and mechanisms of macrophage immunomodulatory activity of a pectic polysaccharide from Cucurbita moschata Duch.

Carbohydr Polym 2021 Oct 2;269:118288. Epub 2021 Jun 2.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China. Electronic address:

A pectic polysaccharide (named CMDP-4b) with a molecular weight of 31.97 kDa was extracted from Cucurbita moschata Duch and purified by column chromatography. On the basis of methylation, Fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-4b was determined to be composed of an α-1,4-linked homogalacturonan backbone, which was slightly acetylated and highly methyl-esterified, and branched at the O-3 position of the →4)-α-D-GalpA-6-OMe-(1→. Read More

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October 2021

Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri.

BMC Biol 2021 Jul 22;19(1):142. Epub 2021 Jul 22.

Division of Infectious Disease, Department of Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.

Background: The opportunistic pathogen Naegleria fowleri establishes infection in the human brain, killing almost invariably within 2 weeks. The amoeba performs piece-meal ingestion, or trogocytosis, of brain material causing direct tissue damage and massive inflammation. The cellular basis distinguishing N. Read More

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Systemic characterization of alternative splicing related to prognosis and immune infiltration in malignant mesothelioma.

BMC Cancer 2021 Jul 22;21(1):848. Epub 2021 Jul 22.

Department of Oncology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian, China.

Background: Malignant mesothelioma (MM) is a relatively rare and highly lethal tumor with few treatment options. Thus, it is important to identify prognostic markers that can help clinicians diagnose mesothelioma earlier and assess disease activity more accurately. Alternative splicing (AS) events have been recognized as critical signatures for tumor diagnosis and treatment in multiple cancers, including MM. Read More

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Influence of a low-head dam on water quality of an urban river system.

J Environ Manage 2021 Jul 23;297:113334. Epub 2021 Jul 23.

Environmental Science, University of Lynchburg, Lynchburg, VA, USA.

Dam removal in the United States is becoming a common practice for stream restoration as these structures age, climate driven precipitation patterns change, and ecological uplift becomes desirable. Yet in highly urbanized watersheds, these dams may operate as retention basins removing pollutants and mitigating hydrological change. While elimination may be ecologically and economically advantageous, sediment and pollutant removal processes may be better protective of water quality and damaging flooding. Read More

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Microstructural Heterogeneity of the Collagenous Network in the Loaded and Unloaded Periodontal Ligament and its Biomechanical Implications.

J Struct Biol 2021 Jul 23:107772. Epub 2021 Jul 23.

School of Aerospace, Mechanical and Mechatronic Engineering, University of Sydney, Sydney, Australia. Electronic address:

The periodontal ligament (PDL) is a highly heterogeneous fibrous connective tissue and plays a critical role in distributing occlusal forces and regulating tissue remodeling. Its mechanical properties are largely determined by the extracellular matrix, comprising a collagenous fiber network interacting with the capillary system as well as interstitial fluid containing proteoglycans. While the phase-contrast micro-CT technique has portrayed the 3D microscopic heterogeneity of PDL, the topological parameters of its network, which is crucial to understanding the multiscale constitutive behavior of this tissue, has not been characterized quantitatively. Read More

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TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Cancer Lett 2021 Jul 23. Epub 2021 Jul 23.

The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China; Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, Fuzhou, China. Electronic address:

The transient receptor potential canonical (TRPC) channels have been implicated in various types of malignancies including gastric cancer (GC). However, the detailed mechanisms of TRPC channels underlying cell proliferation and apoptosis of GC cells remain largely unknown. Here, we report that TRPC3 was highly expressed in clinical GC specimens and correlated with GC malignant progression and poor prognosis. Read More

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