18,811 results match your criteria highly abundant

Axon-enriched lincRNA ALAE is required for axon elongation via regulation of local mRNA translation.

Cell Rep 2021 May;35(5):109053

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China; Shanghai Research Center for Brian Science and Brain-Inspired Intelligence, Shanghai, China. Electronic address:

Long intergenic noncoding RNAs (lincRNAs) are critical regulators involved in diverse biological processes. However, the roles and related mechanisms of lincRNAs in axon development are largely unknown. Here we report an axon-enriched lincRNA regulating axon elongation, referred to as ALAE. Read More

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PA-ResSeg: A Phase Attention Residual Network for Liver Tumor Segmentation from Multi-phase CT Images.

Med Phys 2021 May 5. Epub 2021 May 5.

College of Computer Science and Technology, Zhejiang University, Hangzhou, Zhejiang, China.

Purpose: Liver tumor segmentation is a crucial prerequisite for computer aided diagnosis of liver tumors. In the clinical diagnosis of liver tumors, radiologists usually examine multi-phase CT images as these images provide abundant and complementary information of tumors. However, most known automatic segmentation methods extract tumor features from CT images merely of a single phase, in which valuable multi-phase information is ignored. Read More

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Application of Label-Free Proteomics for Quantitative Analysis of Urothelial Carcinoma and Cystitis Tissue.

Methods Mol Biol 2021 ;2228:283-292

Medizinisches Proteom-Center (MPC) Medical Faculty, Ruhr-University Bochum, Bochum, Germany.

A label-free approach based on a highly reproducible and stable workflow allows for quantitative proteome analysis . Due to advantages compared to labeling methods, the label-free approach has the potential to measure unlimited samples from clinical specimen monitoring and comparing thousands of proteins. The presented label-free workflow includes a new sample preparation technique depending on automatic annotation and tissue isolation via FTIR-guided laser microdissection, in-solution digestion, LC-MS/MS analyses, data evaluation by means of Proteome Discoverer and Progenesis software, and verification of differential proteins. Read More

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January 2021

Identification of First-in-Class Inhibitors of Kallikrein-Related Peptidase 6 That Promote Oligodendrocyte Differentiation.

J Med Chem 2021 May 5. Epub 2021 May 5.

Faculty of Sciences and Engineering, IBPS, UMR 8256 CNRS-UPMC, ERL INSERM U1164, Biological Adaptation and Ageing, Sorbonne Université, F-75252 Paris, France.

Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system (CNS) that causes severe motor, sensory, and cognitive impairments. Kallikrein-related peptidase (KLK)6 is the most abundant serine protease secreted in the CNS, mainly by oligodendrocytes, the myelin-producing cells of the CNS, and KLK6 is assumed to be a robust biomarker of MS, since it is highly increased in the cerebrospinal fluid (CSF) of MS patients. Here, we report the design and biological evaluation of KLK6's low-molecular-weight inhibitors, -aminobenzyl derivatives. Read More

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MnO-capped silk fibroin (SF) nanoparticles with chlorin e6 (Ce6) encapsulation for augmented photo-driven therapy by modulating the tumor microenvironment.

J Mater Chem B 2021 May;9(17):3677-3688

State Key Laboratory of Silkworm Genome Biology, School of Materials and Energy, Southwest University, Chongqing 400715, China.

Silk fibroin (SF), derived from Bombyx mori, is a category of fibrous protein with outstanding potential for applications in the biomedical and biotechnological fields. In spite of its many advantageous properties, the exploration of SF as a versatile nanodrug precursor for tumor therapy has still been restricted in recent years. Herein, a multifunctional SF-derived nanoplatform was facilely developed via encapsulating the photosensitizer chlorin e6 (Ce6) into MnO2-capped SF nanoparticles (NPs). Read More

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A multimer-based SERS aptasensor for highly sensitive and homogeneous assay of carcinoembryonic antigens.

Analyst 2021 May;146(9):3016-3024

School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

Carcinoembryonic antigens (CEAs) are known as one of the most common tumor markers. Their facile and affordable detection is critical for early diagnosis of malignant tumors, especially in resource-constrained settings. Here, we report a novel multimer-based surface-enhanced Raman scattering (SERS) aptasensor for a specific CEA assay. Read More

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Cordycepin inhibits the proliferation of malignant peripheral nerve sheath tumor cells through the p53/Sp1/tubulin pathway.

Am J Cancer Res 2021 15;11(4):1247-1266. Epub 2021 Apr 15.

Institute for Systems Biology Seattle, Washington, USA.

Neurofibromatosis type 1 (NF1) is one of the most common hereditary neurocutaneous disorders. In addition to skin pigmentation and cutaneous neurofibroma, some patients developed the plexiform neurofibroma since birth. Plexiform neurofibroma has abundant Schwann cells, fibroblasts, mast cells, blood vessels, and connective tissues, which increases the risk of developing a malignant peripheral nerve sheath tumor (MPNST). Read More

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Marine Community Metabolomes Carry Fingerprints of Phytoplankton Community Composition.

mSystems 2021 May 4;6(3). Epub 2021 May 4.

School of Oceanography, University of Washington, Seattle, Washington, USA

Phytoplankton transform inorganic carbon into thousands of biomolecules that represent an important pool of fixed carbon, nitrogen, and sulfur in the surface ocean. Metabolite production differs between phytoplankton, and the flux of these molecules through the microbial food web depends on compound-specific bioavailability to members of a wider microbial community. Yet relatively little is known about the diversity or concentration of metabolites within marine plankton. Read More

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DpaA Detaches Braun's Lipoprotein from Peptidoglycan.

mBio 2021 May 4;12(3). Epub 2021 May 4.

Centre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle upon Tyne, United Kingdom

Gram-negative bacteria have a unique cell envelope with a lipopolysaccharide-containing outer membrane that is tightly connected to a thin layer of peptidoglycan. The tight connection between the outer membrane and peptidoglycan is needed to maintain the outer membrane as an impermeable barrier for many toxic molecules and antibiotics. such as covalently attach the abundant outer membrane-anchored lipoprotein Lpp (Braun's lipoprotein) to tripeptides in peptidoglycan, mediated by the transpeptidases LdtA, LdtB, and LdtC. Read More

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The Multifaceted Roles of Zinc in Neuronal Mitochondrial Dysfunction.

Biomedicines 2021 Apr 29;9(5). Epub 2021 Apr 29.

Department of Neurobiology and Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.

Zinc is a highly abundant cation in the brain, essential for cellular functions, including transcription, enzymatic activity, and cell signaling. However, zinc can also trigger injurious cascades in neurons, contributing to the pathology of neurodegenerative diseases. Mitochondria, critical for meeting the high energy demands of the central nervous system (CNS), are a principal target of the deleterious actions of zinc. Read More

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Proteomic and phosphoproteomic profiles of Sertoli cells in buffalo.

Theriogenology 2021 Apr 27;170:1-14. Epub 2021 Apr 27.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China. Electronic address:

Sertoli cells provide nutrients and support for germ cell differentiation and maintain a stable microenvironment for spermatogenesis. Comprehensive identification of Sertoli cellular proteins is important in understanding spermatogenesis. In this study, we performed an integrative analysis of the proteome and phosphoproteome to explore the role of Sertoli cells in spermatogenesis. Read More

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News from Mars: Two-Tier Paradox, Intracellular PCR, Chimeric Junction Shift, Dark Matter mRNA and Other Remarkable Features of Mammalian RNA-Dependent mRNA Amplification. Implications for Alzheimer's Disease, RNA-Based Vaccines and mRNA Therapeutics.

Ann Integr Mol Med 2021 ;2:131-173

Division of Molecular Medicine, Children's Hospital, USA.

Molecular Biology, a branch of science established to examine the flow of information from "letters" encrypted into DNA structure to functional proteins, was initially defined by a concept of DNA-to-RNA-to-Protein information movement, a notion termed the Central Dogma of Molecular Biology. RNA-dependent mRNA amplification, a novel mode of eukaryotic protein-encoding RNA-to-RNA-to-Protein genomic information transfer, constitutes the extension of the Central Dogma in the context of mammalian cells. It was shown to occur in cellular circumstances requiring exceptionally high levels of production of specific polypeptides, e. Read More

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January 2021

Metabolic flexibility allows bacterial habitat generalists to become dominant in a frequently disturbed ecosystem.

ISME J 2021 May 3. Epub 2021 May 3.

Department of Microbiology, Biomedicine Discovery Institute, Clayton, VIC, Australia.

Ecological theory suggests that habitat disturbance differentially influences distributions of habitat generalist and specialist species. While well-established for macroorganisms, this theory has rarely been explored for microorganisms. Here we tested these principles in permeable (sandy) sediments, ecosystems with much spatiotemporal variation in resource availability and physicochemical conditions. Read More

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Targeting autocrine amphiregulin robustly and reproducibly inhibits ovarian cancer in a syngeneic model: roles for wildtype p53.

Oncogene 2021 Apr 30. Epub 2021 Apr 30.

Departments of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel.

Ovarian cancer (OvCA) remains one of the most devastating malignancies, but treatment options are still limited. We report that amphiregulin (AREG) can serve as an effective and safe pharmacological target in a syngeneic murine model. AREG is highly abundant in abdominal fluids of patients with advanced OvCa. Read More

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causing infections in humans are associated with high resistance to heavy metals.

Antimicrob Agents Chemother 2021 May 3. Epub 2021 May 3.

Laboratory of Bacterial Evolution and Molecular Epidemiology, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB-NOVA), Oeiras, Portugal.

is a common pathogen of the urinary tract, a heavy metal-rich environment, but information regarding its heavy metal resistance is unknown.We investigated 422 isolates from human infection and colonization/contamination, animals, and environmental sources for resistance to copper, zinc, arsenic and cadmium using agar dilution method. To identify the genes associated with metal resistance and assess possible links to pathogenicity, we accessed the whole-genome sequence of all isolates and used and pangenome-wide association approaches. Read More

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The Use of Frozen, Food-Grade Blood to Successfully Maintain Colonies of Four Species of Mosquitoes (Diptera: Culicidae).

J Insect Sci 2021 May;21(3)

Lee County Mosquito Control District, 15191 Homestead Road, Lehigh Acres, FL 33971, USA.

An essential component of all mosquito-rearing activities is the act of blood-feeding the mosquitoes (Diptera: Culicidae). Many options exist for this purpose including live host animals and a diverse array of artificial-feeding methods. Most of the published artificial-feeding methods involve expensive materials, custom-built devices, or are labor-intensive. Read More

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Metastable interface biomimetic synthesis of a smart nanosystem for enhanced starvation/gas therapy.

J Colloid Interface Sci 2021 Apr 20;599:149-157. Epub 2021 Apr 20.

The Key Laboratory of Life-Organic Analysis, School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, PR China. Electronic address:

Glucose oxidase (GOx)-mediated starvation therapy holds great promise in cancer treatment. However, the worse hypoxia conditions result into low therapeutic efficiency, and undegradability of carriers poses potential threats to living bodies. To address this, herein a bioinspired MnO nanosystem with controllable surface was developed for highly efficient starvation/gas synergistic enhanced therapy. Read More

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Decreased temperature increases the expression of a disordered bacterial late embryogenesis abundant (LEA) protein that enhances natural transformation.

Virulence 2021 Dec;12(1):1239-1257

Department of Life Technologies, University of Turku, Turku, Finland.

Late embryogenesis abundant (LEA) proteins are important players in the management of responses to stressful conditions, such as drought, high salinity, and changes in temperature. Many LEA proteins do not have defined three-dimensional structures, so they are intrinsically disordered proteins (IDPs) and are often highly hydrophilic. Although LEA-like sequences have been identified in bacterial genomes, the functions of bacterial LEA proteins have been studied only recently. Read More

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December 2021

Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.

Appl Microbiol Biotechnol 2021 May 3. Epub 2021 May 3.

Wuya College of Innovation, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, Liaoning Province, China.

N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines. Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host. In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i. Read More

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N-doped carbon nanotubes supported CoSe nanoparticles: A highly efficient and stable catalyst for HO electrosynthesis in acidic media.

Nano Res 2021 Apr 26:1-6. Epub 2021 Apr 26.

Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, 610054 China.

Electrocatalytic oxygen reduction reaction (ORR) provides an attractive alternative to anthraquinone process for HO synthesis. Rational design of earth-abundant electrocatalysts for HO synthesis via a two-electron ORR process in acids is attractive but still very challenging. In this work, we report that nitrogen-doped carbon nanotubes as a multi-functional support for CoSe nanoparticles not only keep CoSe nanoparticles well dispersed but alter the crystal structure, which in turn improves the overall catalytic behaviors and thereby renders high O-to-HO conversion efficiency. Read More

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Co/CoO heterojunctions encapsulated N-doped carbon sheets via a dual-template-guided strategy as efficient electrocatalysts for rechargeable Zn-air battery.

J Colloid Interface Sci 2021 Apr 20;599:46-57. Epub 2021 Apr 20.

School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, China. Electronic address:

Developing highly efficient oxygen electrocatalysts is of vital importance for rechargeable Zn-air batteries (ZABs). Herein, Co/CoO nano-heterojunctions encapsulated into nitrogen-doped carbon sheets (NCS@Co/CoO) are fabricated via a dual-template-guided approach by using zeolitic imidazolate frameworks (ZIFs) as templates. The synergistic integration of structural and compositional advantages endows such catalyst with superior catalytic properties to benchmark noble-metal catalysts. Read More

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Electrooxidation-enabled electroactive high-valence ferritic species in NiFe layered double hydroxide arrays as efficient oxygen evolution catalysts.

J Colloid Interface Sci 2021 Apr 21;599:168-177. Epub 2021 Apr 21.

Department of Advanced Energy Materials, College of Materials Science and Engineering, Sichuan University, Chengdu 610064, PR China. Electronic address:

Currently, engineering non-precious NiFe layered double hydroxide (NiFe-LDH) electrocatalysts with excellent oxygen evolution performances at high current densities is highly critical to promoting electrolytic water splitting producing hydrogen for large-scale commercial applications. Herein, an intrigued oxygen vacancy-rich Fe(Ⅱ)-incorporated NiFe-LDH containing electroactive high-valence ferritic species is successfully grown on Ni foam (Fe-NiFe-LDH-EO6 h@NF) through an elaborate two-step route including hydrothermal and electrooxidation, and utilized as a high-efficiency elctrocayalyst of alkaline water oxidation possessing abundant exposed active sites, excellent intrinsic catalytic activity and superior durability. Therefore, the Fe-NiFe-LDH-EO6 h@NF electrocatalyst towards oxygen evolution reaction (OER) enables the low overpotentials of 239, 285 and 350 mV for the current densities of 10, 100 and 500 mA cm, respectively, a small Tafel slope of 48. Read More

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Occurrence, distribution, and characterization of suspended microplastics in a highly impacted estuarine wetland in Argentina.

Sci Total Environ 2021 Apr 16;785:147141. Epub 2021 Apr 16.

Instituto Argentino de Oceanografía (IADO), CONICET/UNS, CCT-Bahía Blanca, Camino La Carrindanga, km 7.5, Edificio E1, B8000FWB Bahía Blanca, Buenos Aires, Argentina. Electronic address:

Microplastics have been a global concern due to their potential and widespread risks to organisms and environments. In this study, we investigated the abundance, distribution, and characteristics of microplastics (MPs) in the surface waters of the Bahía Blanca Estuary (BBE), specifically in its inner and middle zone. The results showed the dominant shape of MPs were fibers, being black, transparent, and blue the main colors. Read More

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Carcass decay deteriorates water quality and modifies the nirS denitrifying communities in different degradation stages.

Sci Total Environ 2021 Apr 22;785:147185. Epub 2021 Apr 22.

School of Public Health, Lanzhou University, Lanzhou 730000, China; Center for Grassland Microbiome, Lanzhou University, Lanzhou 730000, China.. Electronic address:

Corpse degradation may release amounts of hazardous materials (e.g., cadaverine, putrescine and ammonia) into surrounding areas, which deteriorate environments and result in nitrogen contamination. Read More

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Synthetic single cell RNA sequencing data from small pilot studies using deep generative models.

Sci Rep 2021 Apr 30;11(1):9403. Epub 2021 Apr 30.

Institute of Medical Biometry and Statistics, Faculty of Medicine and Medical Center, University of Freiburg, 79104, Freiburg, Germany.

Deep generative models, such as variational autoencoders (VAEs) or deep Boltzmann machines (DBMs), can generate an arbitrary number of synthetic observations after being trained on an initial set of samples. This has mainly been investigated for imaging data but could also be useful for single-cell transcriptomics (scRNA-seq). A small pilot study could be used for planning a full-scale experiment by investigating planned analysis strategies on synthetic data with different sample sizes. Read More

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Isolated heterotrophic nitrifying and aerobic denitrifying bacterium for treating actual refinery wastewater with low C/N ratio.

J Biosci Bioeng 2021 Apr 27. Epub 2021 Apr 27.

Hubei Province Key Laboratory of Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China.

Heterotrophic nitrifying and aerobic denitrifying bacteria that have been widely isolated from complicated activated sludge microflorae demonstrate dominant advantages in simultaneous removal of ammonium and nitrogen oxides under aerobic conditions. However, owing to the need of organic carbon to support bacterial growth, nitrogen removal of actual industrial wastewater with low carbon-to-nitrogen (C/N) ratio remains a challenge. Here, Pseudomonas mendocina Y7 was identified and presented to effectively remove nitrogen of actual refinery wastewater with low C/N ratio. Read More

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In situ constructing bismuth nanosheet assembly for highly-selective electrocatalytic CO2 to formate.

Chem Asian J 2021 Apr 30. Epub 2021 Apr 30.

Chinese Academy of Sciences Fujian Institute of Research on the Structure of Matter, State Key Laboratory of Structural Chemistry, 155 Yangqiao Road West, 350002, Fuzhou, CHINA.

The reduction of carbon dioxide (CO2) into value-added fuels using electrochemical method has been regarded as a compelling sustainable energy conversion technology.  However, the high-performance electrocatalysts for CO2 reduction reaction (CO2RR) with high formate selectivity and good stability need to be improved. Earth-abundant Bi has been demonstrated to be active for CO2RR to formate. Read More

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Novel circGFRα1 Promotes Self-Renewal of Female Germline Stem Cells Mediated by mA Writer METTL14.

Front Cell Dev Biol 2021 12;9:640402. Epub 2021 Apr 12.

Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Renji Hospital, School of Medicine, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China.

Circular RNAs (circRNAs) play important roles in the self-renewal of stem cells. However, their significance and regulatory mechanisms in female germline stem cells (FGSCs) are largely unknown. Here, we identified an -methyladenosine (mA)-modified circRNA, circGFRα1, which is highly abundant in mouse ovary and stage-specifically expressed in mouse FGSC development. Read More

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Impact of pesticide/fertilizer mixtures on the rhizosphere microbial community of field-grown sugarcane.

3 Biotech 2021 May 10;11(5):210. Epub 2021 Apr 10.

Institute of Bioengineering, Guangdong Academy of Sciences, No.10 Shiliugang Road, Haizhu District, Guangzhou, 510316 Guangdong China.

The rhizosphere microbial community is important for plant health and is shaped by numerous environmental factors. This study aimed to unravel the effects of a pesticide/fertilizer mixture on the soil rhizosphere microbiome of field-grown sugarcane. A field trial on sugarcane was conducted in Zhanjian City, Guangdong Province, China, and soil samples from the rhizosphere were collected after clothianidin pesticide and/or organic fertilizer treatments. Read More

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Proteomic approach to identify the differentially abundant proteins during flavour development in tuberous roots of Wight & Arn.

3 Biotech 2021 Apr 18;11(4):173. Epub 2021 Mar 18.

Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh India.

2-Hydroxy-4-Methoxy Benzaldehyde (2H4MB) is a structural isomer of vanillin produced in the tuberous roots of . Both vanillin and 2H4MB share the common phenylpropanoid pathway for their synthesis. Unlike vanillin, in which the biosynthetic pathway was well elucidated in , the 2H4MB biosynthetic pathway is not known in any of its plant sources. Read More

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