13,669 results match your criteria higher high-sensitivity


A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator.

J Virol Methods 2021 Sep 15:114289. Epub 2021 Sep 15.

College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea. Electronic address:

A simple reverse transcription loop-mediated isothermal amplification combined with visual detection method (vRT-LAMP) assay was developed for rapid and specific detection of porcine epidemic diarrhea virus (PEDV) in this study, which overcomes the shortcomings of previously described RT-LAMP assays that require additional detection steps or pose a risk of cross-contamination. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubating for 40 min at 62 °C. The assay specifically amplified PEDV RNA and no other viral nucleic acids. Read More

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September 2021

A Biomarker-Based Score for Risk of Hospitalization for Heart Failure in Patients With Diabetes.

Diabetes Care 2021 Sep 17. Epub 2021 Sep 17.

TIMI Study Group, Division of Cardiovascular Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.

Objective: Heart failure (HF) is an impactful complication of type 2 diabetes mellitus (T2DM). We aimed to develop and validate a risk score for hospitalization for HF (HHF) incorporating biomarkers and clinical factor(s) in patients with T2DM.

Research Design And Methods: We derived a risk score for HHF using clinical data, high-sensitivity troponin T (hsTnT), and N-terminal prohormone of B-type natriuretic peptide (NT-proBNP) from 6,106 placebo-treated patients with T2DM in SAVOR-TIMI 53 (Saxagliptin Assessment of Vascular Outcomes Recorded in Patients with Diabetes Mellitus-Thrombolysis in Myocardial Infarction 53). Read More

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September 2021

Diagnostic Accuracy of Clinical Tests and Imaging Exams for Femoroacetabular Impingement: An Umbrella Review of Systematic Reviews.

Clin J Sport Med 2021 Sep 17. Epub 2021 Sep 17.

Department of Surgery, Federal University of Santa Catarina (UFSC), Postgraduate Program in Medical Sciences (PPGCM-UFSC), Florianópolis, Santa Catarina, Brazil; Postgraduate Program in Dentistry, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil; Department of Orthopedics and Traumatology, Celso Ramos Hospital, Florianópolis, Santa Catarina, Brazil; Department of Orthopaedics, Regional Hospital of São José Dr Homero de Miranda Gomes, Florianópolis, Santa Catarina, Brazil; Division of Sports Medicine, Department of Orthopedics, Midwest Orthopedics at Rush, Rush University, Chicago, Illinois; and Internal Medicine Department, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil.

Objective: To synthesize available evidence about diagnostic accuracy of clinical tests and imaging examinations for femoroacetabular impingement (FAI) syndrome.

Design: Umbrella review.

Setting: N/A. Read More

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September 2021

Evaluating sleep quality using the CSHQ-Autism.

Sleep Med 2021 Aug 31;87:69-76. Epub 2021 Aug 31.

Department of Pediatrics, University of Colorado School of Medicine, 13123 E, 16(th)Avenue, Aurora, CO, 80045, USA. Electronic address:

Background: Sleep problems are common in autistic children and adversely impact daytime functioning. The Children's Sleep Habits Questionnaire (CSHQ) [39] was developed from a community-based sample of children and has validated a cut-off score of 41. Katz et al. Read More

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Subclinical myocardial injury and the phenotype of clinical congestion in patients with heart failure and reduced left ventrcular ejection fraction.

J Card Fail 2021 Sep 14. Epub 2021 Sep 14.

Division of Cardiology, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas. Electronic address:

Background: Clinical congestion is associated with adverse outcomes in patients with heart failure. The pathophysiological mediators of this association remain uncertain.

Methods: We prospectively enrolled a cohort of patients with heart failure and reduced left ventricular ejection fraction (LVEF) and performed a detailed clinical examination followed on the same day by an invasive right heart catheterization and blood sampling for biomarkers. Read More

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September 2021

Multivalent Duplexed-Aptamer Networks Regulated a CRISPR-Cas12a System for Circulating Tumor Cell Detection.

Anal Chem 2021 Sep 17. Epub 2021 Sep 17.

Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

Although circulating tumor cells (CTCs) have great potential to act as the mini-invasive liquid biopsy cancer biomarker, a rapid and sensitive CTC detection method remains lacking. CRISPR-Cas12a has recently emerged as a promising tool in biosensing applications with the characteristic of fast detection, easy operation, and high sensitivity. Herein, we reported a CRISPR-Cas12a-based CTC detection sensor that is regulated by the multivalent duplexed-aptamer networks (MDANs). Read More

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September 2021

Synergistic association of the copper/zinc ratio under inflammatory conditions with diabetic kidney disease in patients with type 2 diabetes: The Asahi Diabetes Complications Study.

J Diabetes Investig 2021 Sep 17. Epub 2021 Sep 17.

Division of Diabetes and Metabolism, The Institute of Medical Science, Asahi Life Foundation, Chuo-ku, Tokyo, 103-0002, Japan.

Aims/introduction: We aimed to study the relationships among the copper (Cu)/zinc (Zn) ratio, inflammatory biomarkers, and the prevalence of diabetic kidney disease (DKD) in patients with type 2 diabetes.

Materials And Methods: We performed a cross-sectional study on 651 patients with type 2 diabetes. DKD was defined as a urinary albumin-to-creatinine ratio ≥30 mg/g creatinine and/or an estimated glomerular filtration rate using cystatin C <60 mL/min/1. Read More

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September 2021

Iron deficiency and biomarkers of inflammation: a 3-year prospective analysis of the DO-HEALTH trial.

Aging Clin Exp Res 2021 Sep 17. Epub 2021 Sep 17.

Department of Aging Medicine and Aging Research, University Hospital Zurich and University of Zurich, Raemistrasse 101, 8091, Zurich, Switzerland.

Background: The longitudinal association between iron deficiency and inflammatory biomarkers levels has not been fully explored among relatively healthy older adults.

Aims: To assess whether iron deficiency at baseline and at any yearly follow-up time point, with or without anemia, was associated with changes from baseline in high-sensitivity C-reactive protein (hs-CRP) and interleukin-6 (IL-6) levels over 3 years.

Methods: This is a post-hoc observational analysis of DO-HEALTH, a double-blind, randomized controlled trial including 2157 European community-dwelling adults age 70+. Read More

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September 2021

The effects of insulin therapy on mortality in diabetic patients undergoing percutaneous coronary intervention.

Ann Transl Med 2021 Aug;9(16):1294

Cardiovascular Department, Tianjin Medical University General Hospital, Tianjin, China.

Background: A growing number of studies have reported insulin therapy to be associated with a higher incidence of major adverse cardiac events in diabetic patients with coronary artery disease. However, the relationship between insulin use and the clinical outcomes of patients with diabetes who undergoing percutaneous coronary intervention (PCI) has not been fully clarified.

Methods: A total of 1,069 consecutive patients with diabetes who underwent PCI were enrolled and divided into 2groups: oral hypoglycemic agents (OHA) group (709 patients) and insulin therapy group (360 patients). Read More

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Does the National Immunisation Register stack up? Quantifying accuracy when compared to parent-held health record books.

N Z Med J 2021 Sep 3;134(1541):22-32. Epub 2021 Sep 3.

Director, Immunisation Advisory Centre, Department of General Practice and Primary Health Care, School of Population Health, University of Auckland.

Aim: The National Immunisation Register (NIR), which is derived from general practice management systems, is an important tool for the provision of clinical services, national immunisation programme evaluation and immunisation research in New Zealand. However, the accuracy of the NIR data has not yet been quantified. This study aimed to examine, describe and quantify the extent of discrepancy in the NIR compared to Well Child Tamariki Ora parent-held health record books (Health Books). Read More

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September 2021

Oxidative stress and cardiometabolic biomarkers in patients with non-alcoholic fatty liver disease.

Sci Rep 2021 Sep 16;11(1):18455. Epub 2021 Sep 16.

Department for Medical Biochemistry, University of Belgrade-Faculty of Pharmacy, Belgrade, Serbia.

Oxidative stress is assumed to be the underlying feature of non-alcoholic fatty liver disease (NAFLD). To our knowledge, the mutual involvement of redox status homeostasis parameters [i.e. Read More

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September 2021

Association of triglyceride-glucose index and high-sensitivity C-reactive protein with asymptomatic intracranial arterial stenosis: A cross-sectional study.

Nutr Metab Cardiovasc Dis 2021 Jul 29. Epub 2021 Jul 29.

Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. Electronic address:

Background And Aims: Triglyceride-glucose index (TyG) and high-sensitivity C-reactive protein (hsCRP) have been shown to play important roles in the pathophysiological mechanisms of atherogenesis. However, the cumulative value of TyG and hsCRP in identifying asymptomatic intracranial arterial stenosis (aICAS), as well as its severity and numerical burden, is uncertain. This study seeks to fill this knowledge gap. Read More

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Children-Dietary Inflammatory Index (C-DII), cardiometabolic risk, and inflammation in adolescents: a cross-sectional study.

J Pediatr Endocrinol Metab 2021 Sep 17. Epub 2021 Sep 17.

Department of Nutrition and Dietetics, Fırat University Faculty of Health Sciences, Elazığ, Turkey.

Objectives: There is limited evidence about the inflammatory potential of diet and cardiometabolic risk in children. The aim of this study was to evaluate the association between the Children's Dietary Inflammatory Index (C-DII) with cardiometabolic risk factors in Turkish adolescents from 10 to 17 years.

Methods: Participants aged 10-17 years, who completed a 24-h dietary recall, from which C-DII scores were calculated, were include in this cross-sectional study. Read More

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September 2021

Associations of midchildhood to early adolescence central adiposity gain with cardiometabolic health in early adolescence.

Obesity (Silver Spring) 2021 Sep 16. Epub 2021 Sep 16.

Division of Chronic Disease Research Across the Lifecourse, Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, Massachusetts, USA.

Objective: This study examined the associations of central adiposity gain from midchildhood to early adolescence with cardiometabolic health markers in early adolescence.

Methods: A total of 620 participants were studied in Project Viva. In midchildhood (mean age = 7. Read More

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September 2021

The role of transperineal ultrasound in the evaluation of stress urinary incontinence cases.

Int Braz J Urol 2021 Sep 10;47. Epub 2021 Sep 10.

Department of Obstetrics and Gynecology, University of Health Sciences, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey.

Purpose: To evaluate the use of transperineal ultrasonography while diagnosing stress urinary incontinence (SUI) by comparing the urethral angle (α), posterior urethrovesical angle (β), and bladder neck descent (BND) during rest and Valsalva maneuver in continent women and women with SUI.

Materials And Methods: This prospective observational study was conducted with 50 women with SUI and 50 continent women. Transperineal ultrasonography was performed at rest and during Valsalva maneuver. Read More

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September 2021

Ultrasonographic measurement of the vascular wall thickness and intima-media thickness in patients with Behçet's disease with symptoms or signs of vascular involvement: A cross-sectional study.

Arch Rheumatol 2021 Jun 14;36(2):258-266. Epub 2021 Jan 14.

Department of Internal Medicine, Division of Rheumatology, Pamukkale University Faculty of Medicine, Denizli, Turkey.

Objectives: This study aims to measure and compare bilateral carotid intima-media thickness (CIMT), bilateral jugular, common femoral, and main portal vein wall thicknesses (VWTs) in Behçet's disease (BD) patients with and without vascular involvement to obtain a cut-off value for vascular complications and determine their relationship with disease activity.

Patients And Methods: Sixty-three BD patients (41 males, 22 females; median age: 38.0 years; min 20 - max 71 years) and 30 healthy control subjects (14 males, 16 females; median age: 40. Read More

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HCG18 Participates in Vascular Invasion of Hepatocellular Carcinoma by Regulating Macrophages and Tumor Stem Cells.

Front Cell Dev Biol 2021 30;9:707073. Epub 2021 Aug 30.

Department of Physiology, Medical School of Zhengzhou University, Zhengzhou, China.

Objectives: To identify key genes involved in vascular invasion in hepatocellular carcinoma (HCC), to describe their regulatory mechanisms, and to explore the immune microenvironment of HCC.

Methodology: In this study, the genome, transcriptome, and immune microenvironment of HCC were assessed by using multi-platform data from The Cancer Genome Atlas ( = 373) and GEO data (GSE149614). The key regulatory networks, transcription factors and core genes related to vascular invasion and prognosis were explored based on the CE mechanism. Read More

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Certain dietary patterns are associated with GLIM criteria among Chinese community-dwelling older adults: a cross-sectional analysis.

J Nutr Sci 2021 27;10:e69. Epub 2021 Aug 27.

Department of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.

Disease-related malnutrition is prevalent among older adults; therefore, identifying the modifiable risk factors in the diet is essential for the prevention and management of disease-related malnutrition. The present study examined the cross-sectional association between dietary patterns and malnutrition in Chinese community-dwelling older adults aged ≥65 years in Hong Kong. Dietary patterns, including Diet Quality Index International (DQI-I), Dietary Approaches to Stop Hypertension (DASH), the Mediterranean Diet Score, 'vegetable-fruit' pattern, 'snack-drink-milk product' pattern and 'meat-fish' pattern, were estimated and generated from a validated food frequency questionnaire. Read More

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Two-color, intracavity pump-probe, cavity ringdown spectroscopy.

J Chem Phys 2021 Sep;155(10):104201

Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, California 94550, USA.

We report a proof-of-principle demonstration of intracavity pump-probe, cavity ringdown (CRD) detection in a three-mirror, traveling-wave cavity. With cavity-enhanced pump power and probe absorption path length, the technique is a generally applicable, high-sensitivity, high-selectivity detection method. In our experiments, the pump radiation is switched off during every other probe ringdown, which allows uncorrelated measurements of analyte and background cavity decay rates. Read More

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September 2021

Association of Myocardial Blood Flow Reserve With Adverse Left Ventricular Remodeling in Patients With Aortic Stenosis: The Microvascular Disease in Aortic Stenosis (MIDAS) Study.

JAMA Cardiol 2021 Sep 15. Epub 2021 Sep 15.

Cardiovascular Imaging Program, Division of Cardiovascular Medicine, Division of Cardiac Surgery, Heart and Vascular Center, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.

Importance: Impaired myocardial flow reserve (MFR) and stress myocardial blood flow (MBF) on positron emission tomography (PET) myocardial perfusion imaging may identify adverse myocardial characteristics, including myocardial stress and injury in aortic stenosis (AS).

Objective: To investigate whether MFR and stress MBF are associated with LV structure and function derangements, and whether these parameters improve after aortic valve replacement (AVR).

Design, Setting, And Participants: In this single-center prospective observational study in Boston, Massachusetts, from 2018 to 2020, patients with predominantly moderate to severe AS underwent ammonia N13 PET myocardial perfusion imaging for myocardial blood flow (MBF) quantification, resting transthoracic echocardiography (TTE) for assessment of myocardial structure and function, and measurement of circulating biomarkers for myocardial injury and wall stress. Read More

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September 2021

A simple fluorometric method for rapid screening of aflatoxins after their extraction by magnetic MOF-808/graphene oxide composite and their discrimination by HPLC.

Talanta 2021 Dec 29;235:122709. Epub 2021 Jul 29.

Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

Herein, a high-performance screening tool for the selective and sensitive monitoring of aflatoxins is reported based on their great quenching effect on the blue emission of graphene quantum dots (GQDs). To make a specific determination, a pre-extraction method was also developed using a new nano-sorbent based on the surface-imprinted Zr metal-organic framework on the magnetic graphene oxide (MGO/[email protected]). The adsorbing efficiency of the prepared composite was remarkably higher than the pristine MOF-808 or bare GO. Read More

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December 2021

Diagnostic value of combined serological markers in the detection of acute cerebral infarction.

Medicine (Baltimore) 2021 Sep;100(36):e27146

Department of Clinical Laboratory, Liaocheng People's Hospital, Shandong, China.

Abstract: To evaluate the value of the combination schemes of 10 serological markers in the clinical diagnosis of acute cerebral infarction.The level of total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol, high-sensitivity C-reactive protein, homocysteine (HCY), lipoprotein-related phospholipase A2, ischemia-modified albumin, complement C1q, and lipoprotein a were analyzed in 154 patients with acute ischemic cerebral infarction. The optimized diagnostic combination for acute cerebral infarction was explored by calculating the maximum area under the receiver operating characteristic curves (AUC). Read More

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September 2021

Development and validation of the Brief Parental Burnout Scale (BPBS).

Psychol Assess 2021 Sep 13. Epub 2021 Sep 13.

Department of Psychology, Catholic University of Louvain (UCLouvain).

Since parental burnout can have detrimental consequences for parents, couples, and children, easy-to-use, reliable, and valid practical tools for the early detection of parental burnout are required. We developed and validated a brief scale to identify burned-out parents and parents at burnout risk. In Study 1, we developed the 5-item Brief Parental Burnout scale (BPBs) based on the gold standard measure of parental burnout, the Parental Burnout Assessment (PBA). Read More

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September 2021

Quantitative EEG provides early prediction of poor outcome in acute ischemic stroke after endovascular treatment: a preliminary study.

Neurol Res 2021 Sep 12:1-7. Epub 2021 Sep 12.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

: Quantitative electroencephalogram (QEEG) parameters have been previously utilized in prognosis following acute ischemic stroke (AIS). However, the use and interpretation of QEEG parameters remain scarce following endovascular treatment (EVT) of AIS. AIS patients were prospectively enrolled following EVT, and 24-hour EEG monitoring was conducted. Read More

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September 2021

Hemodiafiltration Is Associated With Reduced Inflammation and Increased Bone Formation Compared With Conventional Hemodialysis in Children: The HDF, Hearts and Heights (3H) Study.

Kidney Int Rep 2021 Sep 6;6(9):2358-2370. Epub 2021 Jul 6.

Pediatric Nephrology Unit, Istanbul University, Faculty of Medicine, Istanbul, Turkey.

Background: Patients on dialysis have a high burden of bone-related comorbidities, including fractures. We report a analysis of the prospective cohort study HDF, Hearts and Heights (3H) to determine the prevalence and risk factors for chronic kidney disease-related bone disease in children on hemodiafiltration (HDF) and conventional hemodialysis (HD).

Methods: The baseline cross-sectional analysis included 144 children, of which 103 (61 HD, 42 HDF) completed 12-month follow-up. Read More

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September 2021

Coagulation and Fibrinolysis Biomarkers as Potential Indicators for the Diagnosis and Classification of Ovarian Hyperstimulation Syndrome.

Front Med (Lausanne) 2021 25;8:720342. Epub 2021 Aug 25.

Department of Clinical Laboratory, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Accurate diagnosis and classification of ovarian hyperstimulation syndrome (OHSS) is important for its management. We employed a new high-sensitivity chemiluminescence immunoassay to detect the thrombin-antithrombin complex (TAT), plasmin alpha2-plasmin inhibitor complex (PIC), soluble thrombomodulin (sTM), and tissue plasminogen activator-inhibitor complex (TPAI-C), and evaluated their diagnostic and classification performance for OHSS. A total of 106 women were enrolled, including 51 patients with OHSS (25 mild or moderate OHSS, 26 severe OHSS), and 55 without OHSS (control group). Read More

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Investigating the depolarization property of skin tissue by degree of polarization uniformity contrast using polarization-sensitive optical coherence tomography.

Biomed Opt Express 2021 Aug 20;12(8):5073-5088. Epub 2021 Jul 20.

Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, BC V6 T 1Z4, Canada.

The depolarization property of skin has been found to be important for skin cancer detection. Previous techniques based on light polarization lack the capability of depth differentiation. Polarization-sensitive optical coherence tomography (PS-OCT) has the advantage of both depth-resolved 3D imaging and high sensitivity to polarization. Read More

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Serum neopterin levels and their role in the prognosis of patients with ischemic stroke: A systematic review and meta-analysis.

Authors:
Lin-Lie Meng Li Cao

J Clin Neurosci 2021 Oct 3;92:55-60. Epub 2021 Aug 3.

Department of Neurology, Nangang Branch, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150001, China. Electronic address:

Background: Neopterin is an inflammatory marker that is found to have prognostic value in cardiovascular diseases but its prognostic role in cerebrovascular diseases is not as clear. This study evaluates the prognostic role of neopterin in patients with ischemic stroke.

Method: Studies were identified after a literature search in electronic databases (Ovid, PubMed, Science Direct, and Wiley) and were selected based on precise eligibility criteria. Read More

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October 2021

A PCR assay with high sensitivity and specificity for the detection of swine toxoplasmosis based on the GRA14 gene.

Vet Parasitol 2021 Sep 4;299:109566. Epub 2021 Sep 4.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, Guangdong Province 510642, People's Republic of China. Electronic address:

Toxoplasma gondii, an intracellular apicomplexan protozoan parasite, can infect all warm-blooded animals. Infected swine are considered one of the most important sources of T. gondii infection in humans. Read More

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September 2021

[Recent advances in glycopeptide enrichment and mass spectrometry data interpretation approaches for glycoproteomics analyses].

Se Pu 2021 Oct;39(10):1045-1054

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Protein glycosylation is one of the most important post-translational modifications (PTMs). The glycosylation is crucial in a variety of physiological and pathological processes that include protein stability, intracellular and intercellular signal transduction, hormone activation or inactivation, and immune regulation. Protein glycosylation is generated by complex biosynthetic pathways comprising hundreds of glycosyltransferases, glycosidases, transcriptional factors, transporters, and protein backbones. Read More

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October 2021