736,526 results match your criteria high levels


Effects of acetylation on dissociation and phosphorylation of actomyosin in postmortem ovine muscle during incubation at 4 °C in vitro.

Food Chem 2021 Mar 27;356:129696. Epub 2021 Mar 27.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, PR China.

This study aimed to assess the effects of acetylation levels on actomyosin disassociation and phosphorylation of lamb during incubation at 4 °C. Samples of whole proteins from lamb longissimus thoracis muscles were prepared and assigned into three treatments (high, middle and low acetylation groups). The results showed that deacetylation of myosin heavy chain and actin was inhibited by lysine deacetylase inhibitor trichostatin A and nicotinamide in this study. Read More

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Analysis of pharmaceutical products and dietary supplements seized from the black market among bodybuilders.

Forensic Sci Int 2021 Mar 30;322:110771. Epub 2021 Mar 30.

Laboratoire de Pharmacologie - Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, FHU Sepsis, AP-HP, 104 boulevard Raymond Poincaré, 92380 Garches, France; Plateforme de Spectrométrie de Masse MassSpecLab, INSERM UMR 1173, UFR des Sciences de la Santé Simone Veil, Université Paris-Saclay (Versailles Saint-Quentin-en-Yvelines), 2 avenue de la source de la Bièvre, 78180 Montigny-le-Bretonneux, France. Electronic address:

Substandard/counterfeit drugs are a growing global problem. According to the World Health Organisation, counterfeit medicines are medicines that are mislabelled deliberately and fraudulently regarding their identity and/or source. In high income countries, drugs seized are mainly represented by performance and image enhancing drugs (PIEDs). Read More

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Prevalence of at-risk hyperandrogenism by age and race/ethnicity among females in the United States using NHANES III.

Eur J Obstet Gynecol Reprod Biol 2021 Mar 26;260:189-197. Epub 2021 Mar 26.

Office of Research Resources, Biostatistics and Epidemiology Consulting Lab, Texas Tech University Health Sciences Center El Paso, El Paso, Texas, USA; Division of Biostatistics & Epidemiology, Department of Molecular and Translational Medicine, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, Texas, USA. Electronic address:

Objective: Hyperandrogenism in females leads to multiple endocrine and metabolic disorders including polycystic ovary syndrome (PCOS) that yields adverse health outcomes across all ages. We sought to estimate the prevalence of hyperandrogenemia and at-risk hyperandrogenism among the US females of different age groups, racial/ethnic, and metabolic characteristics.

Materials And Methods: A retrospective population-based cross-sectional study of females 6 years or older having serum testosterone measures using the National Health and Nutrition Examination Surveys, 2013-2016 was conducted. Read More

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Intramammary infection caused by Staphylococcus aureus increases IgA antibodies to iron-regulated surface determinant-A, -B, and -H in bovine milk.

Vet Immunol Immunopathol 2021 Mar 31;235:110235. Epub 2021 Mar 31.

Dairy Hygiene Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan. Electronic address:

The aim of this study was to identify virulence factors that have high immunogenicity. An in vivo-expressed Staphylococcus aureus antigen was identified by probing bacteriophage expression libraries of S. aureus with antibodies in bovine mastitis milk. Read More

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Internalizing and externalizing problems across childhood and psychotic-like experiences in young-adulthood: The role of developmental period.

Schizophr Res 2021 Apr 7;231:108-114. Epub 2021 Apr 7.

Department of Psychology and Human Development, University College London, London WC1H 0AA, United Kingdom.

Background: Psychopathology in childhood and adolescence, commonly indexed by co-occurring internalizing and externalizing problem behaviors, has been found to predict psychotic-like experiences (PLEs) in adults. However, studies to date have rarely examined internalizing and externalizing problem behaviors simultaneously or identified in which developmental period do these problem behaviors predict PLEs in adults. This study tests to what extent internalizing and externalizing problem behaviors in childhood (4-9 years) or adolescence (11-16 years) predict PLEs in young-adulthood (18 years). Read More

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Effects of low-carbohydrate diet and ketogenic diet on glucose and lipid metabolism in type 2 diabetic mice.

Nutrition 2021 Mar 4;89:111230. Epub 2021 Mar 4.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China. Electronic address:

Objective: With the prevalence of diabetes worldwide, it is urgent to find a suitable treatment. Recently, the ketogenic diet has shown beneficial effects in reducing blood glucose, but some concerns have been raised about its probable side effects, such as hyperlipidemia and hepatic steatosis. Because a low-carbohydrate diet replaces part of the fat with carbohydrates on the basis of the ketogenic diet, we would like to know whether it does better in treating type 2 diabetes. Read More

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The role of the BUN/albumin ratio in predicting mortality in COVID-19 patients in the emergency department.

Am J Emerg Med 2021 Apr 5;48:33-37. Epub 2021 Apr 5.

Emergency Department, Necmettin Erbakan University, Meram School of Medicine, Konya, Turkey.

Introduction: Due to the high mortality and spread rates of coronavirus disease 2019 (COVID-19), there are currently serious challenges in emergency department management. As such, we investigated whether the blood urea nitrogen (BUN)/albumin ratio (BAR) predicts mortality in the COVID-19 patients in the emergency department.

Methods: A total of 602 COVID-19 patients who were brought to the emergency department within the period from March to September 2020 were included in the study. Read More

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Three-dimensional heterogeneity analysis of climate aid's carbon reduction effect.

J Environ Manage 2021 Apr 7;289:112524. Epub 2021 Apr 7.

School of Economics, Wuhan University of Technology, Wuhan, Hubei, 430070, PR China.

Exploring the effectiveness of climate aid is crucial to distribute funds scientifically and maximize its impact. A body of related literature has estimated the impact of international climate aid on carbon emissions. However, these studies did not adequately consider the multi-dimensional heterogeneity of climate aid's carbon reduction effect. Read More

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Extended high frequency audiometry thresholds in healthy school children.

Int J Pediatr Otorhinolaryngol 2021 Mar 23;144:110686. Epub 2021 Mar 23.

Paediatric Research Group, Faculty of Health Sciences, University of Tromsø-Arctic University of Norway, Postboks 6050 Langnes, 9037, Tromsø, Norway; Department of Paediatrics and Adolescence Medicine, University Hospital of North Norway, 9038 Tromsø, Norway. Electronic address:

Objectives: Extended high frequency (EHF) audiometry is the recommended method for monitoring oxotoxic hearing loss in children. This study aims to provide high quality reference audiological data for the EHF range in healthy children.

Methods: Participants were 126 healthy schoolchildren between 6 and 14 years of age. Read More

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Conversion of soluble recalcitrant phosphorus to recoverable orthophosphate form using UV/HO.

Chemosphere 2021 Mar 29;278:130391. Epub 2021 Mar 29.

Department of Civil, Construction and Environmental Engineering, Marquette University, 1637 W. Wisconsin Ave., Milwaukee, WI, 53233, USA. Electronic address:

Soluble non-reactive phosphorus (sNRP), such as inorganic polyphosphates and organic P, is not effectively removed by conventional physicochemical processes. This can impede water resource reclamation facilities' ability to meet stringent total P regulations. This study investigated a UV/HO advanced oxidation process (AOP) for converting sNRP to the more readily removable/recoverable soluble reactive P (sRP), or orthophosphate, form. Read More

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High-Fat Diet Drives an Aggressive Pancreatic Cancer Phenotype.

J Surg Res 2021 Apr 7;264:163-172. Epub 2021 Apr 7.

Department of Surgery, Charleston, South Carolina; Department of Hollings Cancer Center, Charleston, South Carolina; Department of Ralph H. Johnson VA Medical Center, Medical University of South Carolina, Charleston, South Carolina. Electronic address:

Background: Emerging evidence indicates associations between high-fat diet (HFD), metabolic syndrome (MetS), and increased risk of pancreatic cancer. However, individual components of an HFD that increase cancer risk have not been isolated. In addition, a specific pattern of cytokine elevation by which MetS drives pancreatic tumor progression is not well described. Read More

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Exploring unknown per- and polyfluoroalkyl substances in the German environment - The total oxidizable precursor assay as helpful tool in research and regulation.

Sci Total Environ 2021 Mar 29;782:146825. Epub 2021 Mar 29.

German Environment Agency (Umweltbundesamt), 06813 Dessau-Rosslau, Germany.

Limnetic, marine and soil samples of the German environmental specimen bank (ESB) were analyzed for per- and polyfluoroalkyl substances (PFAS) using target analysis and a modified total oxidizable precursor (TOP) assay (direct TOP assay (dTOP)) that works without prior extraction. Target analysis determined ∑PFAS concentrations in bream livers of 8.7-282 μg kg wet weight (ww) in 2019, with highest contaminations in the Rhine and lower Elbe. Read More

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PM and the typical components cause organelle damage, apoptosis and necrosis: Role of reactive oxygen species.

Sci Total Environ 2021 Mar 27;782:146785. Epub 2021 Mar 27.

Environment Research Institute, Shandong University, Qingdao 266237, China. Electronic address:

In this research, the organelle damage, apoptosis and necrosis induced by PM, BC and Kaolin were studied using human bronchial epithelial (16HBE) cells. PM, BC and Kaolin all induce cell death, LDH release and excess intracellular ROS generation. For the organelle injuries, Kaolin and high-dose PM (240 μg/mL) cause lysosomal acidification, but BC causes lysosomal alkalization (lysosomal membrane permeabilization, LMP). Read More

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Development and clinical validation of loop-mediated isothermal amplification (LAMP) assay to diagnose high HBV DNA levels in resource-limited settings.

Clin Microbiol Infect 2021 Apr 7. Epub 2021 Apr 7.

Unité d'Epidémiologie des Maladies Émergentes, Institut Pasteur, Paris, France. Electronic address:

Objectives: A massive scale-up of testing and treatment is indicated to globally eliminate hepatitis B virus (HBV) infection. However, access to a polymerase chain reaction (PCR), a key test to quantify HBV DNA levels and determine treatment eligibility, is limited in resource-limited countries. We developed and evaluated loop-mediated isothermal amplification (LAMP) assay to diagnose clinically important HBV DNA thresholds defined by WHO (≥20,000 and ≥200,000 IU/mL). Read More

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Changes in redox plasma proteome of Pon1-/- mice are exacerbated by a hyperhomocysteinemic diet.

Free Radic Biol Med 2021 Apr 7. Epub 2021 Apr 7.

Department of Biochemistry and Biotechnology, University of Life Sciences, 60-632 Poznań, Poland;; Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University-New Jersey Medical School, International Center for Public Health, Newark, NJ, USA. Electronic address:

High-density lipoprotein (HDL), in addition to promoting reverse cholesterol transport, possesses anti-oxidative, anti-inflammatory, and antithrombotic activities, which are thought to be promoted by paraoxonase 1 (PON1), an HDL-associated enzyme. Reduced levels of PON1 are associated with increased oxidative stress and cardiovascular disease both in humans and Pon1 mice. However, molecular basis of these associations are not fully understood. Read More

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Effect of prenatal phthalate exposure on the association of maternal hormone levels during early pregnancy and reproductive markers in infants at the age of 3 months.

Reprod Toxicol 2021 Apr 7. Epub 2021 Apr 7.

Department of Nursing, Faculty of Social Sciences and Health Care, Constantine the Philosopher University in Nitra, Kraskova 1, 949 74, Nitra, Slovak Republic. Electronic address:

Phthalates belong to the endocrine-disrupting chemicals, altering the hormonal balance in humans during pregnancy with further effects on the reproductive system. This study aimed to investigate the associations between maternal hormone levels during early pregnancy (≤15 week of pregnancy) and reproductive markers in infant boys (n = 37; 61.67%; average age 3. Read More

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Expression of FOXO transcription factors in the brain following traumatic brain injury.

Neurosci Lett 2021 Apr 7:135882. Epub 2021 Apr 7.

Graduate School of Wannan Medical College, Wuhu, 241002, Anhui, China; Department of Pathology, School of Basic Medical Sciences, Wannan Medical College, Wuhu, 241002, Anhui, China. Electronic address:

Traumatic brain injury (TBI) is a substantial clinical and social problem worldwide, causing high morbidity and mortality along with significant economic and medical costs. Forkhead box O transcription factors (FOXOs) have been found to play a critical role in the regulation of cell functions, such as nutrient metabolism, programmed cell death, and tumor suppression. In the central nervous system, FOXOs are reported to be pivotal regulators of learning and memory, neurite outgrowth, and axonal degeneration. Read More

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Effects of high fat diets and supplemental tart cherry and fish oil on obesity and type 2 diabetes in male and female C57BL/6J and TALLYHO/Jng mice.

J Nutr Biochem 2021 Apr 7:108644. Epub 2021 Apr 7.

Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA. Electronic address:

Obesogenic and diabetogenic high fat (HF) diets can influence genetic factors in disease development with sexual dimorphic responses. We investigated potential protective effects of tart cherry (TC), fish oil (FO) and TC+FO supplementation in TALLYHO/Jng (TH) and C57BL/6J (B6) mice fed HF diets. Male and female TH and B6 mice were weaned onto five different diets; low fat (LF), HF, and HF supplemented with TC, FO, or TC+FO and maintained. Read More

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Calcium supplementation relieves high-fat diet-induced liver steatosis by reducing energy metabolism and promoting lipolysis.

J Nutr Biochem 2021 Apr 7:108645. Epub 2021 Apr 7.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, P.R. China. Electronic address:

Nonalcoholic fatty liver disease (NAFLD) is a chronic disease affecting the health of many people worldwide. Previous studies have shown that dietary calcium supplementation may alleviate NAFLD, but the underlying mechanism is not clear. In this study investigating the effect of calcium on hepatic lipid metabolism, 8-week-old male C57BL/6J mice were divided into four groups (n = 6): (a) mice given a normal chow containing 0. Read More

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Olive oil-derived nitro-fatty acids: protection of mitochondrial function in non-alcoholic fatty liver disease.

J Nutr Biochem 2021 Apr 7:108646. Epub 2021 Apr 7.

Departamento de Bioquímica and Centro de Investigaciones Biomédicas (CENIBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. Electronic address:

Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive liver fat deposition in the absence of significant alcohol intake. Since extra virgin olive oil (EVOO) reduces fat accumulation, we analyzed the involvement of nitro-fatty acids (NO-FA) on the beneficial effects of EVOO consumption on NAFLD. Nitro-fatty acids formation was observed during digestion in mice supplemented with EVOO and nitrite. Read More

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Crystal structure of BRP39, a signaling glycoprotein expressed during mammary gland apoptosis at 2.6 Å resolution.

J Struct Biol 2021 Apr 7:107737. Epub 2021 Apr 7.

Department of Chemistry, University of California Davis, Davis, California, 95616, United States; Department of Molecular and Cellular Biology, University of California Davis, Davis, California, 95616, United States.

Breast regression protein 39 (BRP39) is a 39 kDa protein that is a member of chitolectin class of glycosyl hydrolase family 18 (GH18). High expression levels of BRP39 have been detected in breast carcinoma. It helps in proliferation of cells during the progression of this disease and may act as a signaling factor. Read More

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Genome-wide association study of serum metabolites in the African American Study of Kidney Disease and Hypertension.

Kidney Int 2021 Apr 7. Epub 2021 Apr 7.

Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore MD, USA; Welch Center for Prevention, Epidemiology, and Clinical Research, Johns Hopkins University, Baltimore MD, USA; Division of Nephrology, Department of Medicine, Johns Hopkins University, Baltimore MD, USA.

The genome-wide association study (GWAS) is a powerful means to study genetic determinants of disease traits and generate insights into disease pathophysiology. To date, few GWAS of circulating metabolite levels have been performed in African Americans with chronic kidney disease. Hypothesizing that novel genetic-metabolite associations may be identified in a unique population of African Americans with a lower glomerular filtration rate (GFR), we conducted a GWAS of 652 serum metabolites in 619 participants (mean measured glomerular filtration rate 45 mL/min/1. Read More

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Views of health professionals on climate change and health: a multinational survey study.

Lancet Planet Health 2021 Apr 7. Epub 2021 Apr 7.

Department of Public Health, Environmental and Social Determinants of Health, World Health Organization, Geneva, Switzerland.

Climate change arguably represents one of the greatest global health threats of our time. Health professionals can advocate for global efforts to reduce emissions and protect people from climate change; however, evidence of their willingness to do so remains scarce. In this Viewpoint, we report findings from a large, multinational survey of health professionals (n=4654) that examined their views of climate change as a human health issue. Read More

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Screening for asymptomatic diabetes and metabolic comorbidities in pediatric patients during therapy for acute lymphoblastic leukemia.

J Pediatr Endocrinol Metab 2021 Apr 12. Epub 2021 Apr 12.

Department of Pediatric, CHU de Québec-Université Laval, Quebec, Canada.

Objectives: Chronic metabolic disturbances related to cancer treatment are well reported among survivors of pediatric acute lymphoblastic leukemia (ALL). However, few studies have investigated the incidence of these complications during the phase of chemotherapy. We evaluated the incidence of acute metabolic complications occurring during therapy in our cohort of patients diagnosed with ALL. Read More

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Psychosocial Outcomes in Young Adolescents with Type 1 Diabetes Participating in Shared Medical Appointments.

Pediatr Diabetes 2021 Apr 10. Epub 2021 Apr 10.

Center for Endocrinology, Diabetes, and Metabolism, Children's Hospital Los Angeles, Los Angeles, CA.

Objective: For youth with type 1 diabetes (T1D), the early adolescent period is associated with worsening diabetes management and high rates of negative psychosocial issues, including depressive symptoms and family conflict. Alternative clinical models may help improve both diabetes and psychosocial outcomes. Our study aims to investigate whether Team Clinic, a shared medical appointment model developed specifically for adolescents with T1D, will improve psychosocial outcomes for middle school-aged youth. Read More

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CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T).

FEBS Open Bio 2021 Apr 10. Epub 2021 Apr 10.

Department of Science and Environment, Roskilde University, Roskilde, Denmark.

Dysregulation of interleukin 33 (IL-33) has been implicated in the pathogenesis of several autoimmune and inflammatory diseases, but few studies have examined transcriptional regulation of the IL33 gene. In the intestines, gene regulation is controlled by a transcription factor network of which the intestinal-specific transcription factor CDX2 is a key component. In this study, we investigated whether CDX2 regulates IL33 mRNA expression. Read More

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CCR8 marks highly suppressive Treg cells within tumours but is dispensable for their accumulation and suppressive function.

Immunology 2021 Apr 10. Epub 2021 Apr 10.

Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP, Cambridge, UK.

CD4 regulatory T (Treg) cells, dependent upon the transcription factor Foxp3, contribute to tumour immunosuppression but are also required for immune homeostasis. There is interest in developing therapies that selectively target the immunosuppressive function of Treg cells within tumours without disrupting their systemic anti-inflammatory function. High levels of expression of Chemokine (C-C motif) receptor 8 (CCR8) discriminate Treg cells within tumours from those found in systemic lymphoid tissues. Read More

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Sleep symptomatology is associated with greater subjective cognitive concerns: Findings from the community-based Healthy Brain Project.

Sleep 2021 Apr 10. Epub 2021 Apr 10.

The Turner Institute for Brain and Mental Health, Monash University, Clayton, Victoria, Australia.

Study Objectives: To examine if sleep symptomatology was associated with subjective cognitive concerns or objective cognitive performance in a dementia-free community-based sample.

Methods: A total of 1421 middle-aged participants (mean±standard deviation = 57±7; 77% female) from the Healthy Brain Project completed the Pittsburgh Sleep Quality Index (PSQI), Insomnia Severity Index (ISI), and Epworth Sleepiness Scale (ESS) to measure sleep quality, insomnia symptom severity, and daytime sleepiness, respectively. Participants were classified as having no sleep symptomatology (normal scores on each sleep measure), moderate sleep symptomatology (abnormal scores on one sleep measure), or high sleep symptomatology (abnormal scores on at least two sleep measures), using established cut-off values. Read More

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Prognostic value of total testosterone levels in patients with acute coronary syndromes.

Eur J Prev Cardiol 2021 Apr;28(2):235-242

Cardiology Division, Geneva University Hospitals, Switzerland.

Background: Endogenous testosterone levels decrease in men with aging. Controversies persist regarding the screening and treatment of low testosterone levels in patients with acute coronary syndromes (ACS).

Methods And Results: Total serum testosterone levels were measured in 1054 men hospitalized for ACS that were part of a Swiss prospective cohort. Read More

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