4,552 results match your criteria high glucose-induced


Physiology of pancreatic β-cells: Ion channels and molecular mechanisms implicated in stimulus-secretion coupling.

Int Rev Cell Mol Biol 2021 8;359:287-323. Epub 2021 Mar 8.

Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante, Alicante, Spain; Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández, Elche, Spain. Electronic address:

The human and mouse islet of Langerhans is an endocrine organ composed of five different cells types; insulin-secreting β-cells, glucagon-producing α-cells, somatostatin-producing δ-cells, pancreatic polypeptide-secreting PP cells and ɛ-cells that secretes ghrelin. The most important cells are the pancreatic β-cells that comprise around 45-50% of human islets and 75-80% in the mouse. Pancreatic β-cells secrete insulin at high glucose concentration, thereby finely regulating glycaemia by the hypoglycaemic effects of this hormone. Read More

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Adiponectin Modified BMSCs Alleviate Heart Fibrosis via Inhibition TGF-beta1/Smad in Diabetic Rats.

Front Cell Dev Biol 2021 22;9:644160. Epub 2021 Mar 22.

Department of Anatomy, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Accumulating evidence suggested that bone marrow mesenchymal stem cells (BMSCs) have therapeutic potential for diabetes and heart diseases. However, the effects of BMSC on reducing myocardial fibrosis need to be optimized. This study aimed to investigate the mechanism of adiponectin (APN) modified BMSCs on myocardial fibrosis in diabetic model and . Read More

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Silencing circRNA LRP6 down-regulates PRMT1 to improve the streptozocin-induced pancreatic β-cell injury and insulin secretion by sponging miR-9-5p.

J Bioenerg Biomembr 2021 Apr 7. Epub 2021 Apr 7.

Department of Endocrinology and Kidney Internal Medicine, Chonggang General Hospital, No. 1, Dayan Village 3, Dadukou District, Chongqing, 400080, China.

Due to the sedentary lifestyles of people, the number of obese people is increasing alarmingly, which leads to the high prevalence of diabetes mellitus (DM). It was reported that circularRNA (circRNA) LRP6 was upregulated in HG-treated mesangial cells, and it could regulate high glucose-induced cell injury via sponging miR-205. Thus, the aim of this study was to explore the underlying pathogenesis of DM. Read More

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Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM.

Diabetes Metab Syndr Obes 2021 29;14:1445-1451. Epub 2021 Mar 29.

The Third People's Hospital of Yunnan Province, Department of Clinical Laboratory, Kunming, 650200, People's Republic of China.

Purpose: The pancreatic islet specific microRNA-375 (miR-375) is overexpressed in type 2 diabetes mellitus (T2DM) patients suppressing the glucose-induced insulin secretion. Thus, miR-375 may serve as a biomarker for the early prediction of T2DM among high-risk individuals. We conducted this clinical study to assess the significance of miR-375 among type 2 diabetes mellitus (T2DM) patients and their first-degree relatives. Read More

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Deteriorating insulin resistance due to WL15 peptide from cysteine and glycine-rich protein 2 in high glucose induced rat skeletal muscle L6 cells.

Cell Biol Int 2021 Apr 5. Epub 2021 Apr 5.

SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, 603 203, Chennai, Tamil Nadu, India.

This study investigates the antioxidant and antidiabetic activity of the WL15 peptide derived from Channa striatus on regulating antioxidant property in rat skeletal muscle cell line (L6) and enhancing glucose uptake via glucose metabolism. Increased oxidative stress plays a major role in the development of diabetes and its complications. Strategies are needed to mitigate the oxidative stress that can reduce these pathogenic processes. Read More

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MiR-30c-5p/ROCK2 axis regulates cell proliferation, apoptosis and EMT via the PI3K/AKT signaling pathway in HG-induced HK-2 cells.

Open Life Sci 2020 23;15(1):959-970. Epub 2020 Dec 23.

Department of Kidney Disease of Internal, Weihai Central Hospital, No. 3, Mishandong Road West, Wendeng District, 264400, Weihai, China.

Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus. Increasing evidence suggests that microRNA-30c-5p (miR-30c-5p) participates in the pathogenesis of DN, but the mechanism has not been clearly understood. Therefore, this study aimed to investigate the biological role of miR-30c-5p in human DN progression . Read More

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December 2020

Exosomal circEhmt1 Released from Hypoxia-Pretreated Pericytes Regulates High Glucose-Induced Microvascular Dysfunction via the NFIA/NLRP3 Pathway.

Oxid Med Cell Longev 2021 18;2021:8833098. Epub 2021 Mar 18.

Shenzhen Eye Hospital, Shenzhen, 518040 Guangdong, China.

Diabetic retinopathy (DR) is a frequently occurring microvascular complication induced by long-term hyperglycemia. Pericyte-endothelial cell crosstalk is critical for maintaining vascular homeostasis and remodeling; however, the molecular mechanism underlying that crosstalk remains unknown. In this study, we explored the crosstalk that occurs between endothelial cells and pericytes in response to diabetic retinopathy. Read More

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Meteorin-Like Ameliorates β Cell Function by Inhibiting β Cell Apoptosis of and Promoting β Cell Proliferation via Activating the WNT/β-Catenin Pathway.

Front Pharmacol 2021 17;12:627147. Epub 2021 Mar 17.

NHC Key Laboratory of Hormones and Development (Tianjin Medical University), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, Tianjin, China.

Meteorin-like (Metrnl) is a newly discovered myokine. Plasma Metrnl is decreased in subjects with newly diagnosed type 2 diabetes (T2D) and correlated with insulin resistance. This study aims to determine the effects of Metrnl on the apoptosis and proliferation of β cell. Read More

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MiR-1297 attenuates high glucose-induced injury in HK-2 cells via targeting COL1A2.

Nephrology (Carlton) 2021 Apr 2. Epub 2021 Apr 2.

Department of Endocrinology, Zibo Central Hospital, Zibo, China.

Background: In this study, we aimed to explore whether COL1A2 and miR-1297 participated in the progression of diabetic nephropathy (DN) in vitro and classified the underlying mechanisms.

Methods: D-glucose (30 mM; high glucose, HG)-stimulated HK-2 cells were used to mimic DN condition. RNA and non-coding RNA profiles were from Gene Expression Omnibus (GEO) database. Read More

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The triterpene, methyl-3β-hydroxylanosta-9,24-dien-21-oate (RA3), attenuates high glucose-induced oxidative damage and apoptosis by improving energy metabolism.

Phytomedicine 2021 Mar 10;85:153546. Epub 2021 Mar 10.

Biomedical Research and Innovation Platform (BRIP), South African Medical Research Council, Tygerberg 7505, South Africa; Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa. Electronic address:

Background: Hyperglycemia-induced cardiovascular dysfunction has been linked to oxidative stress and accelerated apoptosis in the diabetic myocardium. While there is currently no treatment for diabetic cardiomyopathy (DCM), studies suggest that the combinational use of anti-hyperglycemic agents and triterpenes could be effective in alleviating DCM.

Hypothesis: To investigate the therapeutic effect of methyl-3β-hydroxylanosta-9,24-dien-21-oate (RA3), in the absence or presence of the anti-diabetic drug, metformin (MET), against hyperglycemia-induced cardiac injury using an in vitro H9c2 cell model. Read More

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Rosiglitazone attenuates high glucose-induced proliferation, inflammation, oxidative stress and extracellular matrix accumulation in mouse mesangial cells through the Gm26917/miR-185-5p pathway.

Endocr J 2021 Mar 30. Epub 2021 Mar 30.

Department of Endocrinology, Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Zhuhai, 519000, China.

Rosiglitazone (RSG) is widely used to reduce the amount of sugar in the blood of patients with diabetes mellitus. Diabetic nephropathy is the most common microvascular complication of diabetes. The role of RSG in diabetic nephropathy is not fully understood. Read More

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The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.

Cell Death Dis 2021 Mar 29;12(4):333. Epub 2021 Mar 29.

Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Diabetic nephropathy (DN) is the primary microvascular complication of diabetes mellitus and may result in end-stage renal disease. The overproduction of various inflammatory factors is involved in the pathogenesis of DN. Protein tyrosine phosphatase 1B (PTP1B) modulates the expression of a series of cytokines and nuclear factor kappa B (NF-κB) activity. Read More

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Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function.

Front Pharmacol 2021 10;12:626621. Epub 2021 Mar 10.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, China.

The overall objective of this study was to investigate the effects of catalpol on bone remodeling of diabetic osteoporosis by regulating osteoblast differentiation and migration. Using a murine model of diabetic osteoporosis, to detect the protective effects of catalpol on bone loss, architectural deterioration of trabecular bone and bone metabolism biomarkers were tested. A model of MC3T3-E1 cells was established by treatment with high glucose; the regulatory role of catalpol in the differentiation and migration was tested by Western blot, ALP staining, and Alizarin Red staining. Read More

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Circ_CLASP2 Regulates High Glucose-Induced Dysfunction of Human Endothelial Cells Through Targeting miR-140-5p/FBXW7 Axis.

Front Pharmacol 2021 11;12:594793. Epub 2021 Mar 11.

Department of Cardiovascular, Dongying People's Hospital, Dongying, China.

Hyperglycemia exposure results in the dysfunction of endothelial cells (ECs) and the development of diabetic complications. Circular RNAs (circRNAs) have been demonstrated to play critical roles in EC dysfunction. The current study aimed to explore the role and mechanism of circRNA CLIP-associating protein 2 (circ_CLASP2, hsa_circ_0064772) on HG-induced dysfunction in human umbilical vein endothelial cells (HUVECs). Read More

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Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) Pathway.

Authors:
Yanhua Guan Xu Wang

Med Sci Monit 2021 Mar 26;27:e928153. Epub 2021 Mar 26.

The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China (mainland).

BACKGROUND The primary cause of death in patients with diabetes mellitus (DM) is diabetic macroangiopathy, a complication that related to the function and number of endothelial progenitor cells (EPCs). Salvianic acid A (SAA) is a water-soluble active ingredient of Salvia miltiorrhiza, a traditional Chinese medicine used to treat cardiovascular diseases. The purpose of this study was to explore the effects of SAA on the function of rat EPCs cultured in vitro in a high-glucose environment. Read More

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Hydrogen sulfide ameliorates high glucose-induced pro-inflammation factors in HT-22 cells: Involvement of SIRT1-mTOR/NF-κB signaling pathway.

Int Immunopharmacol 2021 Mar 22;95:107545. Epub 2021 Mar 22.

Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen Biology and Immunology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China; Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China; Department of Biophysics, School of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China. Electronic address:

Hyperglycemia-induced neuroinflammation promotes the progression of diabetic encephalopathy. Hydrogen sulfide (HS) exerts anti-inflammatory and neuroprotective activities against neurodegenerative diseases. However, the effects of HS on hyperglycemia-induced neuroinflammation has not been investigated in neurons. Read More

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Gastrodin protects against high glucose-induced cardiomyocyte toxicity via GSK-3β-mediated nuclear translocation of Nrf2.

Hum Exp Toxicol 2021 Mar 25:9603271211002885. Epub 2021 Mar 25.

Department of Vasculocardiology, The Second People's Hospital of Lianyungang, Lianyungang, Jiangsu, People's Republic of China.

Diabetic cardiomyopathy (DCM) is one of the major complications of diabetes that causes mortality and morbidity in diabetic patients. Gastrodin (GSTD) is a bioactive phenolic glucoside component of an ancient Chinese herb Tianma (Gastrodia elata Bl.), which is widely used for cardiovascular and cerebrovascular diseases by ancient Chinese. Read More

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High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing.

Front Endocrinol (Lausanne) 2021 8;12:603645. Epub 2021 Mar 8.

Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Diabetes-related skin problems represent the most common long-term complications in diabetes mellitus patients. These complications, which include diabetic dermopathy, diabetic blisters, necrobiosis lipoidica diabeticorum, and eruptive xanthomatosis, may dramatically impair patients' quality of life and cause long-lasting disability. However, the cellular and molecular mechanisms linking diabetes-related hyperglycemia and skin complications are still incompletely understood. Read More

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Gut microbiota dysbiosis promotes age-related atrial fibrillation by lipopolysaccharide and glucose-induced activation of NLRP3-inflammasome.

Cardiovasc Res 2021 Mar 23. Epub 2021 Mar 23.

Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin, China.

Aims: Aging is the most significant contributor to the increasing prevalence of atrial fibrillation (AF). The gut microbiota dysbiosis is involved in age-related diseases. However, whether the aged-associated dysbiosis contributes to age-related AF is still unknown. Read More

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Erythropoietin inhibits apoptosis of retinal ganglion cells induced by high glucose through JNK signaling pathway.

J Biol Regul Homeost Agents 2021 Mar 23;35(2). Epub 2021 Mar 23.

Department of Ophthalmology, The First Affiliated Hospital of Jiamusi University, Jiamusi, China.

The aim of this study was to investigate the effect of erythropoietin (EPO) on the apoptosis of retinal ganglion cells (RGCs) induced by high glucose and its mechanism. Rat primary RGCs were extracted to establish high glucose-induced apoptosis models using a 30 mM high-glucose medium. Then flow cytometry, cell counting kit-8 (CCK-8) assay and Western blotting assay were performed to detect the effects of high-, medium- and low-dose EPO on the apoptosis of RGCs induced by high glucose. Read More

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A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis.

Theranostics 2021 20;11(9):4207-4231. Epub 2021 Feb 20.

Department of Physiology, Navy Medical University, Shanghai, China.

Among all the diabetic complications, diabetic cardiomyopathy, which is characterized by myocyte loss and myocardial fibrosis, is the leading cause of mortality and morbidity in diabetic patients. Tissue kallikrein-related peptidases (KLKs) are secreted serine proteases, that have distinct and overlapping roles in the pathogenesis of cardiovascular diseases. However, whether KLKs are involved in the development of diabetic cardiomyopathy remains unknown. Read More

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February 2021

Inhibition of miRNA-155 Alleviates High Glucose-Induced Podocyte Inflammation by Targeting SIRT1 in Diabetic Mice.

J Diabetes Res 2021 8;2021:5597394. Epub 2021 Mar 8.

Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.

Objective: Microinflammation plays a crucial role in podocyte dysfunction in diabetic nephropathy, but its regulatory mechanism is still unclear. This study is aimed at discussing the mechanisms underlying the effect of miRNA-155 on podocyte injury to determine its potential as a therapeutic target.

Methods: Cultured immortalized mouse podocytes and diabetic KK-Ay mice models were treated with a miR-155 inhibitor. Read More

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Resveratrol protects against high glucose-induced oxidative damage in human lens epithelial cells by activating autophagy.

Exp Ther Med 2021 May 26;21(5):440. Epub 2021 Feb 26.

Medical College, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia 028006, P.R. China.

In the pathogenesis of diabetic cataract, high glucose levels induce oxidative damage in human lens epithelial cells (HLECs). Resveratrol has been demonstrated to be a potent antioxidant in various disease conditions; however, limited information is available on its effects on oxidative damage associated with the pathogenesis of diabetic cataract in HLECs. The present study aimed to determine whether resveratrol prevents high glucose-induced oxidative damage to human lens epithelial cells by activating autophagy. Read More

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Quercetin Attenuates Diabetic Peripheral Neuropathy by Correcting Mitochondrial Abnormality via Activation of AMPK/PGC-1α Pathway and .

Front Neurosci 2021 3;15:636172. Epub 2021 Mar 3.

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

The AMPK/PGC-1α pathway-mediated mitochondrial dysfunction has been supposed to play a crucial role in pathogenesis of diabetic peripheral neuropathy (DPN). The present study investigated the neuroprotective potential of quercetin, a natural AMPK activator. Streptozotocin (STZ)-induced diabetic rats that developed DPN phenotype were orally administrated with quercetin (30 and 60 mg/kg per day) for 6 weeks. Read More

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Nifuroxazide improves insulin secretion and attenuates high glucose-induced inflammation and apoptosis in INS-1 cells.

Eur J Pharmacol 2021 Mar 18;899:174042. Epub 2021 Mar 18.

Beijing Diabetes Institute, Beijing Key Laboratory of Diabetes Research and Care, Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. Electronic address:

Inflammation and oxidative stress are important factors that cause islet β-cell dysfunction. STAT3 is not only a major factor in cell proliferation and differentiation, but also plays an important role in mediating inflammation. As a potent inhibitor of STAT3, the effect of Nifuroxazide (Nifu) on pancreatic islet cells in a high glucose environment has not been reported. Read More

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Polysaccharides extracted from Cassia seeds protect against high glucose-induced retinal endothelial cell injury.

Int Ophthalmol 2021 Mar 17. Epub 2021 Mar 17.

Department of Ophthalmology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 365 Renmin East Road, Jinhua, 321000, Zhejiang, China.

Introduction: To investigate the protect effect of polysaccharides extract from cassia seeds (CSPE) on human retinal endothelial cells (HRECs) in hyperglycemia environment.

Methods: The same amount of human retinal endothelial cells (HRECs), respectively, inoculated in vitro were divided into normal group (Con group), hyperglycemia group (H-Glu group), and different concentration of cassia polysaccharides extract (CSPE) combined with high glucose medium group (CSPE group). HRECs in Con group were cultured routinely. Read More

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Protective mechanisms of Taiwanese green propolis toward high glucose-induced inflammation via NLRP3 inflammasome signaling pathway in human gingival fibroblasts.

J Periodontal Res 2021 Mar 17. Epub 2021 Mar 17.

School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.

Objective: To investigate protective effects of Taiwanese green propolis (TGP) against high glucose-induced inflammatory responses in human gingival fibroblasts (HGFs) through NLRP3 inflammasome signaling pathway.

Background: NLRP3 inflammasome has been implicated in the progression of both diabetes mellitus and periodontitis, suggesting a common potential therapeutic target for these diseases. Propolis is renowned for various biological activities, particularly anti-inflammation and antioxidant, representing a promising therapy for many conditions. Read More

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The Long Non-Coding RNA HOTAIR Is a Critical Epigenetic Mediator of Angiogenesis in Diabetic Retinopathy.

Invest Ophthalmol Vis Sci 2021 Mar;62(3):20

Department of Pathology and Laboratory Medicine, Western University, London, Ontario, Canada.

Purpose: Diabetic retinopathy (DR) remains a pressing issue worldwide. Abnormal angiogenesis is a distinct vascular lesion in DR, and research has established that vascular endothelial growth factor A (VEGF-A) is a primary mediator of such changes. However, limitations in current anti-VEGF therapies suggest that our understanding of molecular networks underlying ocular angiogenesis remains far from complete. Read More

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Alpha-mangostin improves endothelial dysfunction in db/db mice through inhibition of aSMase/ceramide pathway.

J Cell Mol Med 2021 Apr 14;25(7):3601-3609. Epub 2021 Mar 14.

Xiangya Hospital of Central South University, Changsha, China.

Diabetic vascular complications are the leading causes of death and disability in patients with diabetes. Alpha-mangostin has been reported to have anti-diabetic capacity in recent years. Here, we investigated the protective function of alpha-mangostin on endothelium in vitro and in vivo experiments. Read More

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