34,984 results match your criteria heterologous expression

NemA Catalyzes Trivalent Organoarsenical Oxidation and Is Regulated by the Trivalent Organoarsenical-Selective Transcriptional Repressor NemR.

Environ Sci Technol 2021 Apr 14. Epub 2021 Apr 14.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China.

Synthetic aromatic arsenicals such as roxarsone (Rox(V)) and nitarsone (Nit(V)) have been used as animal growth enhancers and herbicides. Microbes contribute to redox cycling between the relatively less toxic pentavalent and highly toxic trivalent arsenicals. In this study, we report the identification of operon from . Read More

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Prostaglandin production by the microalga with heterologous expression of cyclooxygenase.

Biotechnol Bioeng 2021 Apr 14. Epub 2021 Apr 14.

Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.

Prostaglandins (PGs) are the physiologically active compounds synthesized from C20 polyunsaturated fatty acids (PUFAs) by cyclooxygenase (COX) and a series of PG synthases, and are utilized as pharmaceuticals. Currently commercialized PGs are mainly produced by chemical synthesis under harsh conditions. By contrast, bioproduction of PGs can be an alternative, environmental-friendly, and inexpensive process with genetic engineering of model plants, although these conventional host organisms contain the limited quantity of PG precursors. Read More

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Cloning, heterologous expression, and characterization of a novel thioesterase from natural sample.

Heliyon 2021 Mar 24;7(3):e06542. Epub 2021 Mar 24.

Biochemistry Research Group, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung, Indonesia.

A novel thioesterse gene was successfully cloned and sequenced directly from natural sample of Domas Hot Spring, West Java, Indonesia. Homological analysis of the sequence showed that the gene appeared high homology to thioesterase genes with the highest to a putative thioesterase gene from uncultured at 66% identity. However, phylogenetic analysis showed that the protein was separated from the branch with other known thioesterases. Read More

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Mutation of cysteine residues increases heterologous expression of peach expansin in the methylotrophic yeast .

Plant Biotechnol (Tokyo) 2020 Dec;37(4):397-403

Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

The study of Carbohydrate-Active enZymes (CAZymes) associated with plant cell wall metabolism is important for elucidating the developmental mechanisms of plants and also for the utilization of plants as a biomass resource. The use of recombinant proteins is common in this context, but heterologous expression of plant proteins is particularly difficult, in part because the presence of many cysteine residues promotes denaturation, aggregation and/or protein misfolding. In this study, we evaluated two phenotypes of methylotrophic yeast as expression hosts for expansin from peach ( (L. Read More

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December 2020

The efficacy of CRISPR-mediated cytosine base editing with the RPS5a promoter in Arabidopsis thaliana.

Sci Rep 2021 Apr 13;11(1):8087. Epub 2021 Apr 13.

Center for Genome Engineering, Institute for Basic Science, Daejeon, 34126, Republic of Korea.

CRISPR/Cas9-mediated genome editing is an important and versatile technology in modern biological research. Recent advancements include base-editing CRISPR tools that enable targeted nucleotide substitutions using a fusion protein comprising a nickase variant of Cas9 and a base deaminase. Improvements in base editing efficiencies and inheritable of edited loci need to be made to make CRISPR a viable system in plants. Read More

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In vivo uptake of a fluorescent conjugate of melanin-concentrating hormone in the rat brain.

J Chem Neuroanat 2021 Apr 10:101959. Epub 2021 Apr 10.

Departamento de Fisiología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. Electronic address:

Melanin-concentrating hormone (MCH) is a hypothalamic neuropeptide synthesized by posterior hypothalamic and incerto-hypothalamic neurons that project throughout the central nervous system. The MCHergic system modulates several important functions such as feeding behavior, mood and sleep. MCH exerts its biological functions through interaction with the MCHR-1 receptor, the only functional receptor present in rodents. Read More

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A Natural Product Chemist's Guide to Unlocking Silent Biosynthetic Gene Clusters.

Annu Rev Biochem 2021 Apr 13. Epub 2021 Apr 13.

Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA; email:

Microbial natural products have provided an important source of therapeutic leads and motivated research and innovation in diverse scientific disciplines. In recent years, it has become evident that bacteria harbor a large, hidden reservoir of potential natural products in the form of silent or cryptic biosynthetic gene clusters (BGCs). These can be readily identified in microbial genome sequences but do not give rise to detectable levels of a natural product. Read More

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Droplet-Based Microfluidic High-Throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica.

Methods Mol Biol 2021 ;2307:205-219

Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.

Yarrowia lipolytica has emerged as an attractive solution for screening enzyme activities thanks to the numerous tools available for heterologous protein production and its strong secretory ability. Nowadays, activity screening for improved enzymes mostly relies on the evaluation of independent clones in microtiter plates. However, even with highly robotized screening facilities, the relatively low throughput and high cost of the technology do not enable the screening of large diversities, which significantly reduce the probability of isolating improved variants. Read More

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January 2021

An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters.

Sci Rep 2021 Apr 12;11(1):7854. Epub 2021 Apr 12.

Biochemical Evolution Laboratory, Department of Animal Science, University of California, Davis, CA, 95616, USA.

CRISPR/Cas9 gene editing is effective in manipulating genetic loci in mammalian cell cultures and whole fish but efficient platforms applicable to fish cell lines are currently limited. Our initial attempts to employ this technology in fish cell lines using heterologous promoters or a ribonucleoprotein approach failed to indicate genomic alteration at targeted sites in a tilapia brain cell line (OmB). For potential use in a DNA vector approach, endogenous tilapia beta Actin (OmBAct), EF1 alpha (OmEF1a), and U6 (TU6) promoters were isolated. Read More

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Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic .

ACS Synth Biol 2021 Apr 12. Epub 2021 Apr 12.

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695, United States.

is a probiotic yeast that exhibits rapid growth at 37 °C, is easy to transform, and can produce therapeutic proteins in the gut. To establish its ability to produce small molecules encoded by multigene pathways, we measured the amount and variance in protein expression enabled by promoters, terminators, selective markers, and copy number control elements. We next demonstrated efficient (>95%) CRISPR-mediated genome editing in this strain, allowing us to probe engineered gene expression across different genomic sites. Read More

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Ca-activated Cl channel TMEM16A inhibition by cholesterol promotes angiogenesis in endothelial cells.

J Adv Res 2021 Mar 15;29:23-32. Epub 2020 Sep 15.

Department of Ion Channel Pharmacology, School of Pharmacy, China Medical University, Shenyang 110122, China.

Introduction: Ca-activated Cl channel TMEM16A is expressed in endothelial cells, and contributes to many diseases such as hypertension, blood-brain barrier dysfunction, and pulmonary hypertension. It remains unclear whether TMEM16A regulates endothelial angiogenesis, which participates in many physiological and pathological processes. Cholesterol regulates many ion channels including TMEM16A, and high cholesterol levels contribute to endothelial dysfunction. Read More

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Genetic suppression of lethal mutations in fatty acid biosynthesis mediated by a secondary lipid synthase.

Appl Environ Microbiol 2021 Apr 9. Epub 2021 Apr 9.

Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA USA

The biosynthesis and incorporation of polyunsaturated fatty acids into phospholipid membranes is a unique feature of certain marine Gammaproteobacteria inhabiting high-pressure and/or low temperature environments. In these bacteria, monounsaturated and saturated fatty acids are produced via the classical dissociated Type II fatty acid synthase mechanism, while omega-3 polyunsaturated fatty acids such as EPA (20:53) and DHA (22:63) are produced by a hybrid polyketide/fatty acid synthase - encoded by the genes - also referred to as the secondary lipid synthase mechanism. In this work, phenotypes associated with partial or complete loss of monounsaturated biosynthesis are shown to be compensated for by several-fold increased production of polyunsaturated fatty acids in the model marine bacterium SS9. Read More

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Proteolytic maturation of the Outer Membrane -type cytochrome OmcZ by Subtilisin-like Serine Protease is Essential for Optimal Current Production by .

Appl Environ Microbiol 2021 Apr 9. Epub 2021 Apr 9.

Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

An outer membrane -type cytochrome (OmcZ) in is essential for optimal current production in microbial fuel cells. OmcZ exists in two forms, small and large, designated as OmcZ and OmcZ, respectively. However, it is still not known how these two structures are formed. Read More

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Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters.

ACS Synth Biol 2021 Apr 9. Epub 2021 Apr 9.

Center for Synthetic Biology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.

One major challenge in synthetic biology is the deleterious impacts of cellular stress caused by expression of heterologous pathways, sensors, and circuits. Feedback control and dynamic regulation are broadly proposed strategies to mitigate this cellular stress by optimizing gene expression levels temporally and in response to biological cues. While a variety of approaches for feedback implementation exist, they are often complex and cannot be easily manipulated. Read More

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Global Genome Mining Reveals a Cytochrome P450-Catalyzed Cyclization of Crownlike Cyclodipeptides with Neuroprotective Activity.

Org Lett 2021 Apr 8. Epub 2021 Apr 8.

Department of Ocean Science and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory, The Hong Kong University of Science and Technology, Hong Kong, China.

We conducted global genome mining of 162,672 bacterial genomes and identified 829 cyclodipeptide (CDP) biosynthesis gene clusters (BGC) containing a cytochrome P450 gene. Heterologous expression of BGC from DSM 44795 led to the identification of two novel crownlike CDPs, cyctetryptomycin A () and B (), which possess unprecedented complex macrocycle and show neuroprotective activity. The two cytochrome P450s found in the BGC catalyze sequential reactions leading to the cyclization of diketopiperazine dimers. Read More

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The P1 Protein of Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of .

Front Microbiol 2021 22;12:645530. Epub 2021 Mar 22.

Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB Bellaterra, Barcelona, Spain.

Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with significant increases in accumulation of the non-potyvirus partner, as in the case of melon plants infected by the potyvirus (WMV) and the crinivirus (CYSDV). To further explore the synergistic interaction between these two viruses, the activity of RNA silencing suppressors (RSSs) was addressed in transiently co-expressed combinations of heterologous viral products in leaves. While the strong RSS activity of WMV Helper Component Proteinase (HCPro) was unaltered, including no evident additive effects observed when co-expressed with the weaker CYSDV P25, an unexpected negative effect of WMV P1 was found on the RSS activity of P25. Read More

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Characterization of Transport Activity of SLC11 Transporters in Oocytes by Fluorophore Quenching.

SLAS Discov 2021 Apr 7:24725552211004123. Epub 2021 Apr 7.

University of Insubria, Varese, Lombardia, Italy.

Membrane proteins are involved in different physiological functions and are the target of pharmaceutical and abuse drugs. oocytes provide a powerful heterologous expression system for functional studies of these proteins. Typical experiments investigate transport using electrophysiology and radiolabeled uptake. Read More

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Class A Penicillin-Binding Protein-Mediated Cell Wall Synthesis Promotes Structural Integrity during Peptidoglycan Endopeptidase Insufficiency in Vibrio cholerae.

mBio 2021 04 6;12(2). Epub 2021 Apr 6.

Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, New York, USA

The bacterial cell wall is composed primarily of peptidoglycan (PG), a poly-aminosugar that is essential to sustain cell shape, growth, and structural integrity. PG is synthesized by class A/B penicillin-binding proteins (a/bPBPs) and shape, elongation, division, and sporulation (SEDS) proteins like RodA (as part of the Rod system cell elongation machinery) and degraded by "autolytic" enzymes to accommodate growth processes. It is thought that autolysins (particularly endopeptidases [EPs]) are required for PG synthesis and incorporation by creating gaps that are patched and paved by PG synthases, but the exact relationship between autolysins and PG synthesis remains incompletely understood. Read More

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Genome Mining-Driven Discovery of 5-Methylorsellinate-Derived Meroterpenoids from .

Org Lett 2021 Apr 6. Epub 2021 Apr 6.

Department of Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong SAR, China.

Heterologous expression of a cryptic gene cluster in the fungus CBS 116.56 led to the discovery of four new meroterpenoids, funiculolides A-D (-), derived from the aromatic polyketide 5-methylorsellinic acid (5-MOA). Intriguingly, funiculolide D (), the apparent end product of the pathway, harbors an unusual spirocyclopentanone moiety, which is synthesized by the oxidative rearrangement catalyzed by the ferrous iron and α-ketoglutarate-dependent dioxygenase FncG. Read More

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OsLEA1a overexpression enhances tolerance to diverse abiotic stresses by inhibiting cell membrane damage and enhancing ROS scavenging capacity in transgenic rice.

Funct Plant Biol 2021 Apr 6. Epub 2021 Apr 6.

Late embryogenesis abundant (LEA) proteins are involved in diverse abiotic stresses tolerance in many different organisms. Our previous studies have shown that the heterologous expression of OsLEA1a interfered with the resistance of Escherichia coli to abiotic stresses. However, in the present study, based on growth status and physiological indices of rice plant, the overexpression of OsLEA1a in rice conferred increased resistance to abiotic stresses compared with the wild-type (WT) plants. Read More

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Identification of two UDP-glycosyltransferases involved in the main oleanane-type ginsenosides in Panax japonicus var. major.

Planta 2021 Apr 5;253(5):91. Epub 2021 Apr 5.

State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, National and Local Joint Engineering Research Center on Gemplasm Utilization and Innovation of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, 650201, Yunnan, People's Republic of China.

Main Conclusion: Two UDP-glycosyltransferases from Panax japonicus var. major were identified, and the biosynthetic pathways of three oleanane-type ginsenosides (chikusetsusaponin IVa, ginsenoside Ro, zingibroside R) were elucidated. Chikusetsusaponin IVa and ginsenoside Ro are primary active components formed by stepwise glycosylation of oleanolic acid in five medicinal plants of the genus Panax. Read More

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Heterologous Expression and Partial Characterization of a New Alanine Dehydrogenase from Amycolatopsis sulphurea.

Fatih Aktaş

Protein J 2021 Apr 5. Epub 2021 Apr 5.

Faculty of Engineering, Düzce University, 81600, Düzce, Turkey.

A novel alanine dehydrogenase (AlaDH; EC.1.4. Read More

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Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts.

Plant Cell Rep 2021 Apr 3. Epub 2021 Apr 3.

Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, 1 Kent St, Bentley, WA, 6102, Australia.

Key Message: Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful. Agroinfiltration is a valuable research method for investigating virulence and avirulence effector proteins from pathogens and pests, where heterologous effector proteins are transiently expressed in plant leaves and hypersensitive necrosis responses and other effector functions can be assessed. Read More

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Genome Mining, Heterologous Expression, Antibacterial and Antioxidant Activities of Lipoamides and Amicoumacins from Compost-Associated fmb60.

Molecules 2021 Mar 26;26(7). Epub 2021 Mar 26.

College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.

fmb60, which has broad-spectrum antimicrobial activities, was isolated from plant straw compost. A hybrid NRPS/PKS cluster was screened from the genome. Sixteen secondary metabolites produced by the gene cluster were isolated and identified using LC-HRMS and NMR. Read More

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as a Potential Adjuvant and Delivery System for the Development of SARS-CoV-2 Oral Vaccines.

Microorganisms 2021 Mar 26;9(4). Epub 2021 Mar 26.

Laboratory of Animal Products Chemistry, Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.

The most important characteristics regarding the mucosal infection and immune responses against the Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) as well as the current vaccines against coronavirus disease 2019 (COVID-19) in development or use are revised to emphasize the opportunity for lactic acid bacteria (LAB)-based vaccines to offer a valid alternative in the fight against this disease. In addition, this article revises the knowledge on: (a) the cellular and molecular mechanisms involved in the improvement of mucosal antiviral defenses by beneficial strains, (b) the systems for the expression of heterologous proteins in and (c) the successful expressions of viral antigens in that were capable of inducing protective immune responses in the gut and the respiratory tract after their oral administration. The ability of to express viral antigens, including the spike protein of SARS-CoV-2 and its capacity to differentially modulate the innate and adaptive immune responses in both the intestinal and respiratory mucosa after its oral administration, indicates the potential of this LAB to be used in the development of a mucosal COVID-19 vaccine. Read More

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Deletion of Alters the Function of the Na1.5 Channel in Murine Cardiac Myocytes.

Int J Mol Sci 2021 Mar 26;22(7). Epub 2021 Mar 26.

Institute of Biochemistry and Molecular Medicine, and Swiss National Centre of Competence in Research (NCCR) TransCure, University of Bern, Bühlstrasse 28, 3012 Bern, Switzerland.

Transient receptor potential melastatin member 4 (TRPM4) encodes a Ca-activated, non-selective cation channel that is functionally expressed in several tissues, including the heart. Pathogenic mutants in have been reported in patients with inherited cardiac diseases, including conduction blockage and Brugada syndrome. Heterologous expression of mutant channels in cell lines indicates that these mutations can lead to an increase or decrease in TRPM4 expression and function at the cell surface. Read More

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Genetically Encoded Self-Assembling Iron Oxide Nanoparticles as a Possible Platform for Cancer-Cell Tracking.

Pharmaceutics 2021 Mar 16;13(3). Epub 2021 Mar 16.

Department of Medical Nanobiotechnology, Pirogov Russian National Research Medical University, 117997 Moscow, Russia.

The study of growth and possible metastasis in animal models of tumors would benefit from reliable cell labels for noninvasive whole-organism imaging techniques such as magnetic resonance imaging. Genetically encoded cell-tracking reporters have the advantage that they are contrast-selective for viable cells with intact protein expression machinery. Besides, these reporters do not suffer from dilution during cell division. Read More

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Non-Target Effects of dsRNA Molecules in Hemipteran Insects.

Genes (Basel) 2021 Mar 12;12(3). Epub 2021 Mar 12.

Department of Entomology, Cornell University, Ithaca, NY 14850, USA.

Insect pest control by RNA interference (RNAi)-mediated gene expression knockdown can be undermined by many factors, including small sequence differences between double-stranded RNA (dsRNA) and the target gene. It can also be compromised by effects that are independent of the dsRNA sequence on non-target organisms (known as sequence-non-specific effects). This study investigated the species-specificity of RNAi in plant sap-feeding hemipteran pests. Read More

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Chimeric VLPs Based on HIV-1 Gag and a Fusion Rabies Glycoprotein Induce Specific Antibodies against Rabies and Foot-and-Mouth Disease Virus.

Vaccines (Basel) 2021 Mar 12;9(3). Epub 2021 Mar 12.

Departament d'Enginyeria Química, Biològica i Ambiental, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.

Foot and mouth disease is a livestock acute disease, causing economic losses in affected areas. Currently, control of this disease is performed by mandatory vaccination campaigns using inactivated viral vaccines. In this work, we describe the development of a chimeric VLP-based vaccine candidate for foot-and-mouth disease virus (FMDV), based on the co-expression of the HIV-1 Gag protein and a novel fusion rabies glycoprotein (RVG), which carries in its N-term the FMDV main antigen: the G-H loop. Read More

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In Silico Prediction and Analysis of Unusual Lantibiotic Resistance Operons in the Genus .

Microorganisms 2021 Mar 19;9(3). Epub 2021 Mar 19.

Institute of Microbiology and Biotechnology, Ulm University, 89081 Ulm, Germany.

Post-translationally modified, (methyl-)lanthionine-containing peptides are produced by several Gram-positive bacteria. These so-called lantibiotics have potent activity against various bacterial pathogens including multidrug-resistant strains and are thus discussed as alternatives to antibiotics. Several naturally occurring mechanisms of resistance against lantibiotics have been described for bacteria, including cell envelope modifications, ABC-transporters, lipoproteins and peptidases. Read More

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