4 results match your criteria hesc-ec induction

  • Page 1 of 1

VEGF Promotes Endothelial Cell Differentiation from Human Embryonic Stem Cells Mainly Through PKC-ɛ/η Pathway.

Stem Cells Dev 2020 01 23;29(2):90-99. Epub 2019 Dec 23.

Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.

Human embryonic stem cells (hESCs) have unlimited proliferation capacity and can differentiate into most types of somatic cells. We previously described that the overexpression of FLI1 as well as the activation of protein kinase C (FLI1-PKC) could rapidly and efficiently differentiate hESCs into endothelial cells (ECs). However, the relationship between vascular endothelial growth factor (VEGF) and PKC in hESC-EC differentiation is debated, and the roles of different PKC isoforms in hESC-EC differentiation remain unknown. Read More

View Article and Full-Text PDF
January 2020

Notch hyper-activation drives trans-differentiation of hESC-derived endothelium.

Stem Cell Res 2016 09 13;17(2):391-400. Epub 2016 Sep 13.

Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, New York, NY 10065, United States; Tri-Institutional Stem Cell Derivation Laboratory, Weill Cornell Medical College, New York, NY 10065, United States. Electronic address:

During development, endothelial cells (EC) display tissue-specific attributes that are unique to each vascular bed, as well as generic signaling mechanisms that are broadly applied to create a patent circulatory system. We have previously utilized human embryonic stem cells (hESC) to generate tissue-specific EC sub-types (Rafii et al., 2013) and identify pathways that govern growth and trans-differentiation potential of hESC-derived ECs (James et al. Read More

View Article and Full-Text PDF
September 2016

The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function.

J Cell Sci 2014 Apr 24;127(Pt 7):1428-40. Epub 2014 Jan 24.

Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing, China.

The key regulators of endothelial differentiation that is induced by shear stress are mostly unclear. Human atonal homolog 6 (Hath6 or ATOH8) is an endothelial-selective and shear-stress-responsive transcription factor. In this study, we sought to elucidate the role of Hath6 in the endothelial specification of embryonic stem cells. Read More

View Article and Full-Text PDF

Improvement of postnatal neovascularization by human embryonic stem cell derived endothelial-like cell transplantation in a mouse model of hindlimb ischemia.

Circulation 2007 Nov 5;116(21):2409-19. Epub 2007 Nov 5.

Department of Bioengineering, Hanyang University, Seoul, Korea.

Background: We established an efficient preparation method to obtain endothelial-like cells (ECs) from human embryonic stem cells (hESCs) and tested whether these hESC-ECs would show therapeutic potential for treatment of hindlimb ischemia.

Methods And Results: ECs differentiated from hESCs were obtained by mechanical isolation and cell sorting for von Willebrand factor. The isolated hESC-ECs maintained endothelial cell-specific characteristics such as endothelial marker expression and capillary formation. Read More

View Article and Full-Text PDF
November 2007
  • Page 1 of 1