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The Improvement Effect of Sodium Ferulate on the Formation of Pulmonary Fibrosis in Silicosis Mice Through the Neutrophil Alkaline Phosphatase 3 (NALP3)/Transforming Growth Factor-β1 (TGF-β1)/α-Smooth Muscle Actin (α-SMA) Pathway.

Med Sci Monit 2021 Jun 15;27:e927978. Epub 2021 Jun 15.

Department of Occupational Disease, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, China (mainland).

BACKGROUND Pneumoconiosis is a chronic progressive fibrotic interstitial pneumonia for which the pathogenesis and treatment remain unclear. Previous studies showed that sodium ferulate (SF) may have a therapeutic effect, and this study explored the mechanism underlying SF-related improvement. MATERIAL AND METHODS In this study, a silicosis mouse model and primary cultured mouse lung fibroblasts were established. Read More

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Expression of VEGF-A Signaling Pathway in Cartilage of ACLT-induced Osteoarthritis Mouse Model.

J Orthop Surg Res 2021 Jun 14;16(1):379. Epub 2021 Jun 14.

Laboratory for New Techniques of Restoration & Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.

Background: Anterior cruciate ligament transection surgery (ACLT)-induced OA model was often used to investigate the molecular mechanism of knee osteoarthritis (KOA). Researches have shown that vascular endothelial growth factor (VEGF) played an important role in OA. The present study aimed to investigate the pathological changes after ACLT surgery and reveal the expression characteristics of the VEGF-A/VEGFR2 signaling pathway in this model. Read More

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Schisantherin A ameliorates liver fibrosis through TGF-β1mediated activation of TAK1/MAPK and NF-κB pathways in vitro and in vivo.

Phytomedicine 2021 Jul 24;88:153609. Epub 2021 May 24.

Department of Pharmacology, College of Pharmacy, Beihua University, No. 3999 Binjiang East Road, Jilin, Jilin Province, 132013, China. Electronic address:

Backgroud: Schisandra chinensis, a traditional Chinese medicine for liver protection, can significantly improve liver fibrosis. However, it is still unclear which active components in Schisandra chinensis play an anti-fibrosis role.

Purpose: The purpose of present study was to observe the anti-fibrosis effect of schisantherin A (SCA) on liver fibrosis and explore its underlying mechanism. Read More

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Contribution of M-opsin-based color vision to refractive development in mice.

Exp Eye Res 2021 Jun 12;209:108669. Epub 2021 Jun 12.

Department of Ophthalmology, Eye & ENT Hospital Affiliated to Fudan University, Shanghai, China; Department of Ophthalmology, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China. Electronic address:

M-opsin, encoded by opn1mw gene, is involved in green-light perception of mice. The role of M-opsin in emmetropization of mice remains uncertain. To answer the above question, 4-week-old wild-type (WT) mice were exposed to white light or green light (460-600 nm, a peak at 510 nm) for 12 weeks. Read More

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Effect of Local Delivery of Vancomycin and Tobramycin on Bone Regeneration.

Orthop Surg 2021 Jun 14. Epub 2021 Jun 14.

Department of Traumatology, Beijing Jishuitan Hospital, Beijing, China.

Objective: A bone defect rat model was established to investigate the osteogenic effect of local delivery two antibiotics (vancomycin and tobramycin powder) on bone regeneration.

Methods: Twenty-four Sprague-Dawley (SD) male rats (6 to 8 weeks, 200 to 250 g) were used in this study. All these rats were randomly divided into four groups. Read More

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Resveratrol improves cardiac function and left ventricular fibrosis after myocardial infarction in rats by inhibiting NLRP3 inflammasome activity and the TGF-1/SMAD2 signaling pathway.

PeerJ 2021 28;9:e11501. Epub 2021 May 28.

Jiangsu Vocational College of Medicine, Yancheng, Jiangsu, China.

Background: Several studies have shown that resveratrol (RES), a naturally occurring polyphenol found in many plants, is beneficial for preventing cardiovascular diseases. However, the mechanism underlying the RES-mediated protection against myocardial infarction has not yet been revealed entirely. In this study, we investigated the protective effects of RES on cardiac function in a rat model of acute myocardial infarction (AMI) and the related underlying mechanisms. Read More

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Comparative Studies on Volatile Oil Components Derived from Integrated Processing Technology of Origin and Traditional Cutting Processing Technology of Moslae Herba and Their Effects on Rats With Lung-Yin Deficiency.

Evid Based Complement Alternat Med 2021 25;2021:5557222. Epub 2021 May 25.

College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Shenyang, China.

This study aimed to examine the components of the volatile oil derived from the integration processing technology of origin (IPTO) of Moslae Herba (MH) and their effects on the treatment of rats with Lung-Yin deficiency. IPTO was compared with the traditional cutting processing technology (TCPT) to provide a feasible basis. The chemical constituents of the volatile oil were identified by gas chromatography-mass spectrometry (GC-MS). Read More

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Peritumoral Tertiary Lymphoid Structures Correlate With Protective Immunity and Improved Prognosis in Patients With Hepatocellular Carcinoma.

Front Immunol 2021 26;12:648812. Epub 2021 May 26.

Department of Liver Surgery, Liver Transplantation Division, West China Hospital, Sichuan University, Chengdu, China.

The existence of intratumoral tertiary lymphoid structure (iTLS) has been reported to correlative with favorable clinical outcomes for patients with hepatocellular carcinoma (HCC). However, little is known about the role of peritumoral TLS (pTLS). This study aimed to investigate the prognostic role of pTLS either alone or jointly with iTLS and the potential association with local immune response in HCC. Read More

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Total Flavonoids of Rhizoma Drynariae Promotes Differentiation of Osteoblasts and Growth of Bone Graft in Induced Membrane Partly by Activating Wnt/β-Catenin Signaling Pathway.

Front Pharmacol 2021 26;12:675470. Epub 2021 May 26.

First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.

Total flavonoids of Rhizoma drynariae (TFRD), a Chinese medicine, is widely used in the treatment of fracture, bone defect, osteoporosis and other orthopedic diseases, and has achieved good effects. Purpose of this trial was to explore efficacy of TFRD on bone graft's mineralization and osteoblasts' differentiation in Masquelet induced membrane technique in rats. Forty male Sprague-Dawley rats were randomly divided into high dose group (H-TFRD), middle dose group (M-TFRD), low dose group (L-TFRD) and control group (control). Read More

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Paeonol Induces Protective Autophagy in Retinal Photoreceptor Cells.

Front Pharmacol 2021 26;12:667959. Epub 2021 May 26.

Eye Institute, Eye and ENT Hospital, College of Medicine, Fudan University, Shanghai, China.

Retinal photoreceptor (RP) cells are widely involved in retina-related diseases, and oxidative stress plays a critical role in retinal secondary damage. Herein, we investigated the effectiveness and potential mechanisms of autophagy of paeonol (Pae) in terms of oxidation resistance. The animal model was induced by light damage (LD) , whereas the model was established by HO stimulation. Read More

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Inhibition of angiotensin II type 1 receptor by candesartan reduces tumor growth and ameliorates fibrosis in colorectal cancer.

EXCLI J 2021 7;20:863-878. Epub 2021 May 7.

Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Colorectal cancer (CRC) is an important cause of cancer-related mortality. Aberrant activation of the renin-angiotensin system (RAS) is reported to be associated with poor clinical outcomes in patients with CRC. This study was designed to explore the anti-tumor effects of the angiotensin receptor blocker Candesartan either alone or in combination with 5-FU in and models of CRC. Read More

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Redefining chronic endometritis: the importance of endometrial stromal changes.

Fertil Steril 2021 Jun 10. Epub 2021 Jun 10.

Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, Illinois. Electronic address:

Objective: To develop diagnostic criteria for chronic endometritis and compare the prevalence of chronic endometritis between women with recurrent pregnancy loss (RPL) and controls.

Design: Cohort study.

Setting: Single academic fertility center. Read More

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Evaluation of Villus Synovium From Unaffected Metacarpophalangeal Joints of Adult and Juvenile Horses.

J Equine Vet Sci 2021 Jul 29;102:103637. Epub 2021 Apr 29.

Deparment for horses, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.

Horses are a widely accepted model for osteoarthritis (OA) research. Synovial tissue sampling is commonly used in studies to evaluate and grade the progress of OA or to assess treatment effects. Synovial explants play an important role in ex-vivo studies, increasingly replacing the use of living animals. Read More

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The effect of Raphanus sativus L. seeds on regulation of intestinal motility in rats consuming a high-calorie diet.

Biomed Pharmacother 2021 Jun 9;140:111705. Epub 2021 Jun 9.

School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing, PR China. Electronic address:

Purpose: The purpose of this study was to explore the effects of a short-term high-calorie diet and the regulation mechanism of Raphanus sativus L. seeds (RSL seeds) on the intestinal motility of young rats.

Methods: We fed 20 Specific Pathogen Free (SPF) 4-week-old male Sprague-Dawley (SD) rats special high-calorie diet for 3 days and then randomized them to a high-calorie diet group (HCG, 10 rats) and an RSL seeds treatment group (TG, 10 rats). Read More

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Development of a novel model of intervertebral disc degeneration by the intradiscal application of monosodium iodoacetate (MIA) in rat Degenerative change in monosodium iodoacetate (MIA)-injected intervertebral disc.

Spine J 2021 Jun 9. Epub 2021 Jun 9.

Department of Physiology, Korea University, Seoul, Republic of Korea. Electronic address:

Background Context: Low back pain is one of the most common musculoskeletal disorders. While the pathology of intervertebral disc (IVD) degeneration has been modeled using various biological methods, these models are inadequate for simulating similar pathological states in humans.

Purpose: This study investigated whether monosodium iodoacetate (MIA) injection into the IVD of rats could generate a reliable model of IVD degeneration. Read More

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Silencing of transcription factor E93 inhibits adult morphogenesis and disrupts cuticle, wing and ovary development in Locusta migratoria.

Insect Sci 2021 Jun 11. Epub 2021 Jun 11.

Research Institute of Applied Biology, Shanxi University, Taiyuan, China.

Ecdysone-induced protein 93F (E93) plays important roles during the metamorphosis process in insects. In this study, a cDNA of the LmE93 gene was identified from the transcriptome of Locusta migratoria, which consists of the 3378-nucleotide open-reading frame (ORF) and encodes 1125 amino acids with helix-turn-helix (HTH) motifs. Reverse transcription quantitative polymerase chain reaction analysis revealed that LmE93 was highest expressed in ovary. Read More

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595-nm pulsed dye laser combined with fractional CO laser reduces hypertrophic scar through down-regulating TGFβ1 and PCNA.

Lasers Med Sci 2021 Jun 12. Epub 2021 Jun 12.

Department of Dermatology, Seventh Medical Center of PLA General Hospital, Beijing, 100700, China.

595-nm pulsed dye laser and fractional CO laser have been demonstrated effective to treat hypertrophic scar. The underlying mechanism may involve transforming growth factor-beta1 (TGFβ1) and proliferating cell nuclear antigen (PCNA), but remains to be clarified. Our study was performed to investigate how 595-nm pulsed dye laser combined with fractional CO laser treats hypertrophic scars in a rabbit model through regulating the expression of TGFβ1 and PCNA. Read More

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Prognostic impact of histological review of high-grade endometrial carcinomas in a large Danish cohort.

Virchows Arch 2021 Jun 11. Epub 2021 Jun 11.

Department of Pathology, Copenhagen University Hospital, Herlev and Gentofte Hospital, Copenhagen University, Borgmester Ib Juuls Vej 73, 2730, Herlev, Denmark.

The aim of this study was to investigate the outcome of histological subtype review of high-grade endometrial carcinoma (EC) and its prognostic impact in a large well-documented Danish nationwide cohort. From the Danish Gynecological Cancer Database (DGCD) 2005-2012 cohort, we included 425 patients with an original diagnosis of high-grade EC, independent of histologic subtype. Of these, at least one hematoxylin and eosin (H&E)-stained slide from 396 cases (93. Read More

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Future of biomarker evaluation in the realm of artificial intelligence algorithms: application in improved therapeutic stratification of patients with breast and prostate cancer.

J Clin Pathol 2021 Jul 11;74(7):429-434. Epub 2021 Jun 11.

School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Dublin, Ireland.

Clinical workflows in oncology depend on predictive and prognostic biomarkers. However, the growing number of complex biomarkers contributes to costly and delayed decision-making in routine oncology care and treatment. As cancer is expected to rank as the leading cause of death and the single most important barrier to increasing life expectancy in the 21st century, there is a major emphasis on precision medicine, particularly individualisation of treatment through better prediction of patient outcome. Read More

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Thrombotic Pathology is not Correlated with the Prognosis of Endovascular Treatment for Acute Ischemic Stroke.

J Stroke Cerebrovasc Dis 2021 Jun 8;30(8):105755. Epub 2021 Jun 8.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400042 Chongqing, China. Electronic address:

Objective: The aim of the present study was to determine whether there is a correlation between thrombotic pathology and prognosis of endovascular treatment (EVT) for acute ischemic stroke (AIS).

Methods: Thrombi were taken from 58 patients with cerebral ischemic thrombosis who were consecutively selected for EVT for AIS. The collected thrombi then underwent hematoxylin-eosin staining for pathological examinations to determine the red blood cell (RBC) ratio and fibrin/platelet components. Read More

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Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China.

Poult Sci 2021 Apr 29;100(7):101210. Epub 2021 Apr 29.

Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province; Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science Sichuan University, Chengdu 610064, China. Electronic address:

Avian infectious bronchitis (IB), caused by avian infectious bronchitis virus (IBV), is an acute and highly contagious disease that is extremely harmful to the poultry industry throughout the world. The cross-using of different attenuated live vaccine strains has led to the occurrence of diverse IBV serotypes. In this study, we isolated an IBV strain from a chicken farm in southwest China and designated it CK/CH/SCMY/160315. Read More

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The pattern of duck sternal ossification and the changes of histological structure and gene expression therein.

Poult Sci 2021 Mar 12;100(7):101112. Epub 2021 Mar 12.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.

As the largest single bone, avian sterna are very different from those of mammals in terms of morphology and functions. Moreover, years of artificial selection in poultry led to incomplete sternal ossification at slaughter age, which may cause diseases, sternal injury, and restriction to breast muscle growth. However, in living birds, studies have rarely described the ossification pattern and underlying mechanisms of the sterna. Read More

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Exercise attenuates angiotensinⅡ-induced muscle atrophy by targeting PPARγ/miR-29b.

J Sport Health Sci 2021 Jun 8. Epub 2021 Jun 8.

Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School of Life Science, Shanghai University, Shanghai 200444, China; Shanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai 200444, China. Electronic address:

Background: Exercise is beneficial for muscle atrophy. Peroxisome proliferator-activated receptor gamma (PPARγ) and microRNA-29b (miR-29b) have been reported to be responsible for angiotensin Ⅱ (AngⅡ)-induced muscle atrophy. However, it is unclear whether exercise can protect AngⅡ-induced muscle atrophy by targeting PPARγ/miR-29b. Read More

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Kidney Injury in a Hemodilution Model of Hemorrhagic Shock and Fluid Resuscitation.

Am J Med Sci 2021 Jun 8. Epub 2021 Jun 8.

Department of Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, P. R. China. Electronic address:

Background: Fluid therapy is indispensable in treating patients with hemorrhagic shock. However, fluid overload correlates with kidney injury in patients with hemorrhagic shock. We hypothesized that hemodilution after fluid treatment contributes to the kidney injury. Read More

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Beneficial role of Pistacia lentiscus aqueous extract in experimental colitis: anti-inflammatory and potential therapeutic effects.

Inflammopharmacology 2021 Jun 11. Epub 2021 Jun 11.

Team "Cytokines and NO Synthases", LBCM (Laboratory of Cellular and Molecular Biology), FSB (Faculty of Biological Science), USTHB (University of Sciences and Technology Houari Boumediene), BP 32, El-Alia, 16111, Algiers, Algeria.

Background: Pistacia lentiscus L. (PL) is a flowering plant traditionally used in the treatment of gastrointestinal disorders. The extracts of this plant are endowed with strong pharmacological activities. Read More

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Icariin contributes to healing skull defects in rabbit model.

J Tradit Chin Med 2021 Jun;41(3):471-478

Department of Oral and maxillofacial surgery, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, 050017, China.

Objective: To investigate the efficacy of icariin for healing skull defects in rabbit models.

Methods: Thirty-six 3-month-old female New Zealand white rabbits, weighing 1.8-2. Read More

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Electroacupuncture Pretreatment at Neiguan (PC6) attenuates autophagy in rats with myocardial ischemia reperfusion through the phosphatidylinositol 3-kinase-Akt-mammalian target of rapamycin pathway.

J Tradit Chin Med 2021 Jun;41(3):455-462

Institute of Internal medicine, Huangjiahu Hospital of Hubei University of Traditional Chinese Medicine, Wuhan 430065, China.

Objective: To investigate the protective efficacy of electroacupuncture (EA) pretreatment at Neiguan (PC6) on myocardial ischemia-reperfusion (I/R) in rats.

Methods: Fifty rats were randomly divided into five groups (n = 10): sham operation group, model group (underwent in vivo myocardial I/R), EA pretreatment group [EA at Neiguan (PC 6) 1 week before I/R], wortmannin group (1 week before I/R, the PI3K inhibitor, wortmannin, was injected), EA pretreatment + wortmannin group (both pretreatments were performed simultaneously). After establishing the I/R model, 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to analyze the weight and area of the myocardial infarction tissue. Read More

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Full composition granules of Huanglian (Rhizoma Coptidis) decrease the serum monocyte chemotactic protein-1 and connective tissue growth factor levels and inhibit kidney nuclear factor-κB expression in rats with high-fat diet-induced diabete.

J Tradit Chin Med 2021 Jun;41(3):424-431

Department of Traditional Chinese Medicine, the Mongolian Medical and Traditional Chinese Medicine Hospital of Wuhai, Wuhai 016000, China.

Objective: To investigate the efficacy of the full composition granules of Huanglian (Rhizoma Coptidis) (FGC) on the serum monocyte chemotactic protein-1 (MCP-1) and connective tissue growth factor (CTGF) levels and kidney nuclear factor-κB (NF-κB) expression in rats with high-fat diet-induced diabetes.

Methods: Diabetes was induced in rats by feeding a high-fat chow combined with intravenous streptozotocin injection. Forty diabetic Sprague- Dawley rats were randomly assigned to a normal group (NG), model group (MG), irbesartan group (IG), and low-, middle-, and high-dosage FGC groups (LFGC, MFGC, HFGC), with eight rats per group. Read More

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Gyejibokryeong-Hwan improves recovery of injured muscles in mouse model of muscle contusion.

J Tradit Chin Med 2021 Jun;41(3):406-413

Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daejeon University, Daejeon 34520, Republic of Korea.

Objective: To investigate the effects of Gyejibokryeong-Hwan (Guizhifuling-wan, GBH) on muscle injury in a mouse model of muscle contusion.

Methods: C57/BL6 mouse biceps femoris muscles were injured using the drop-mass method and injured animals were treated orally with GBH (50, 100, or 500 mg/kg) once a day for 7 d. Open field and treadmill running tests were performed to assess functional recovery from muscle injury. Read More

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Celastrol induces caspase-dependent apoptosis of hepatocellular carcinoma cells by suppression of mammalian target of rapamycin.

J Tradit Chin Med 2021 Jun;41(3):381-389

The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Objective: To investigate the efficacy of celastrol treatment of hepatocellular carcinoma (HCC) cells in vitro and in vivo and to propose a mechanism of action.

Methods: A human HepG2 liver cancer cell line and a xenograft tumor model were used to investigate the effects of celastrol on HCC in vitro and in vivo. A CCK-8 kit was used to detect cell viability. Read More

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