129,830 results match your criteria hematopoietic stem


Novel GNE Gene Variants Associated with Severe Congenital Thrombocytopenia and Platelet Sialylation Defect.

Thromb Haemost 2022 Jan 20. Epub 2022 Jan 20.

Translational Glycomics Center, Versiti Blood Research Institute, Milwaukee, Wisconsin, United States.

The gene encodes an enzyme that initiates and regulates the biosynthesis of -acetylneuraminic acid, a precursor of sialic acids. GNE mutations are classically associated with Nonaka myopathy and sialuria, following an autosomal recessive and autosomal dominant inheritance pattern. Reports show that single GNE variants cause severe thrombocytopenia without muscle weakness. Read More

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January 2022

Reduced intensity hematopoietic stem cell transplantation for myelofibrosis in accelerated-phase.

Blood Adv 2022 Jan 20. Epub 2022 Jan 20.

University Medical Center Hamburg_Eppendorf, Hamburg, Germany.

Accelerated-phase (AP) myelofibrosis, currently defined by circulating blasts 10-19%, usually confers very high risk for progression and poor outcome. The outcome of hematopoietic stem cell transplantation for AP myelofibrosis has not been evaluated yet. We analyzed the outcome of 349 clinically and genetically annotated patients with primary or secondary myelofibrosis undergoing reduced intensity transplantation, of whom 35 had AP myelofibrosis. Read More

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January 2022

Anti-Xa based dosing of enoxaparin in hematopoietic stem cell transplant and adoptive cell therapy patients: A single center experience.

Thromb Res 2022 Jan 15;211:6-9. Epub 2022 Jan 15.

Cleveland Clinic Taussig Cancer Institute, Cleveland, OH, United States of America. Electronic address:

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January 2022

Cadmium exposure reprograms energy metabolism of hematopoietic stem cells to promote myelopoiesis at the expense of lymphopoiesis in mice.

Ecotoxicol Environ Saf 2022 Jan 17;231:113208. Epub 2022 Jan 17.

School of Public Health and Key Laboratory of Public Health Safety, MOE, Fudan University, Shanghai 200032, China. Electronic address:

Cadmium (Cd) is a highly toxic heavy metal in our living environment. Hematopoietic stem cells (HSC) are ancestors for all blood cells. Therefore understanding the impact of Cd on HSC is significant for public health. Read More

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January 2022

Acute Myeloid Leukemia Stem Cells: Origin, Characteristics, and Clinical Implications.

Stem Cell Rev Rep 2022 Jan 20. Epub 2022 Jan 20.

Division of Hematology and Oncology, Department of Medicine, Pennsylvania State University College of Medicine, Hershey, PA, USA.

The stem cells of acute myeloid leukemia (AML) are the malignancy initiating cells whose survival ultimately drives growth of these lethal diseases. Here we review leukemia stem cell (LSC) biology, particularly as it relates to the very heterogeneous nature of AML and to its high disease relapse rate. Leukemia ontogeny is presented, and the defining functional and phenotypic features of LSCs are explored. Read More

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January 2022

High-throughput Imaging of CRISPR- and Recombinant Adeno-associated Virus-induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells.

CRISPR J 2022 Jan 20. Epub 2022 Jan 20.

Institute of Nanotechnology and Advanced Materials, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.

CRISPR-Cas technology has revolutionized gene editing, but concerns remain due to its propensity for off-target interactions. This, combined with genotoxicity related to both CRISPR-Cas9-induced double-strand breaks and transgene delivery, poses a significant liability for clinical genome-editing applications. Current best practice is to optimize genome-editing parameters in preclinical studies. Read More

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January 2022

Red cell distribution width as a predictor of outcome in hospitalized cirrhotic patients.

Eur J Gastroenterol Hepatol 2021 Dec;33(1S Suppl 1):e978-e985

Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebin El-Kom.

Background: A systemic inflammatory response syndrome (SIRS) is linked to red cell distribution width (RCDW), which produces pro-inflammatory signals that act directly on hematopoietic stem cells in the bone marrow. This stimulation may cause alterations in the membrane of red blood cells (RBCs), as assessed by RCDW, which have been linked to increased morbidity and death in a number of systemic disorders.

Aim: This study aims to evaluate RCDW as a predictor of outcome in hospitalized cirrhotic patients. Read More

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December 2021

Measurable residual disease, FLT3-ITD mutation, and disease status have independent prognostic influence on outcome of allogeneic stem cell transplantation in NPM1-mutated acute myeloid leukemia.

Cancer Med 2022 Jan 20. Epub 2022 Jan 20.

Department of Hematology, EBMT Paris Office, CEREST-TC, Saint Antoine Hospital, Paris, France.

Nucleophosmin-1 (NPM1) mutations in acute myeloid leukemia (AML) confer a survival advantage in the absence of FLT3-internal tandem duplication (FLT3-ITD). Here, we investigated the main predictors of outcome after allogeneic hematopoietic stem cell transplantation (allo-HCT). We identified 1572 adult (age ≥ 18 year) patients with NPM1-mutated AML in first complete remission (CR1:78%) or second complete remission (CR2:22%) who were transplanted from matched sibling donors (30. Read More

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January 2022

The Path to an Evidence-Based Treatment Protocol for Extraoral Photobiomodulation Therapy for the Prevention of Oral Mucositis.

Front Oral Health 2021 16;2:689386. Epub 2021 Jul 16.

Department of Surgery, Divisions of Oral Medicine and Dentistry, Brigham and Women's Hospital and the Dana-Farber Cancer Institute, Boston, MA, United States.

Oral mucositis is a painful complication of hematopoietic stem cell transplantation for which photobiomodulation therapy (PBMT) is a safe and effective intervention. Extraoral delivery of PBMT has clinical advantages over intraoral delivery but requires additional dosimetric considerations due to the external tissue layers through which the light must propagate before reaching the oral mucosa. Additionally, to date there has been no dose modeling study, a task essential to developing a justified treatment protocol. Read More

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Low frequency of treatable pediatric disease alleles in gnomAD: An opportunity for future genomic screening of newborns.

HGG Adv 2022 Jan 25;3(1):100059. Epub 2021 Sep 25.

Harvard Medical School, Boston, MA, USA.

Hematopoietic stem cell transplant (HSCT) can prevent progression of several genetic disorders. Although a subset of these disorders are identified on newborn screening panels, others are not identified until irreversible symptoms develop. Genetic testing is an efficient methodology to ascertain pre-symptomatic children, but the penetrance of risk-associated variants in the general population is not well understood. Read More

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January 2022

Altered BMP2/4 Signaling in Stem Cells and Their Niche: Different Cancers but Similar Mechanisms, the Example of Myeloid Leukemia and Breast Cancer.

Front Cell Dev Biol 2021 3;9:787989. Epub 2022 Jan 3.

CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France.

Understanding mechanisms of cancer development is mandatory for disease prevention and management. In healthy tissue, the microenvironment or niche governs stem cell fate by regulating the availability of soluble molecules, cell-cell contacts, cell-matrix interactions, and physical constraints. Gaining insight into the biology of the stem cell microenvironment is of utmost importance, since it plays a role at all stages of tumorigenesis, from (stem) cell transformation to tumor escape. Read More

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January 2022

Bone Marrow Mesenchymal Stromal Cells: Identification, Classification, and Differentiation.

Front Cell Dev Biol 2021 3;9:787118. Epub 2022 Jan 3.

Department of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Medical University, Shanghai, China.

Bone marrow mesenchymal stromal cells (BMSCs), identified as pericytes comprising the hematopoietic niche, are a group of heterogeneous cells composed of multipotent stem cells, including osteochondral and adipocyte progenitors. Nevertheless, the identification and classification are still controversial, which limits their application. In recent years, by lineage tracing and single-cell sequencing, several new subgroups of BMSCs and their roles in normal physiological and pathological conditions have been clarified. Read More

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January 2022

Single-Cell RNA-Seq of Bone Marrow Cells in Aplastic Anemia.

Front Genet 2021 3;12:745483. Epub 2022 Jan 3.

Institute of Hematology, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.

Aplastic anemia (AA) is an autoimmune disease characterized by peripheral blood pancytopenia and bone marrow failure. Recently, a research study verified bone marrow failure of AA patients resulting from hematopoietic stem and progenitor cell (HSPC) attack by active T cells. Nonetheless, whether B cells, as one of the important immune cells, destruct the hematopoiesis is still unclear. Read More

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January 2022

Natural Barcodes for Longitudinal Single Cell Tracking of Leukemic and Immune Cell Dynamics.

Front Immunol 2021 3;12:788891. Epub 2022 Jan 3.

Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, United States.

Blood malignancies provide unique opportunities for longitudinal tracking of disease evolution following therapeutic bottlenecks and for the monitoring of changes in anti-tumor immunity. The expanding development of multi-modal single-cell sequencing technologies affords newer platforms to elucidate the mechanisms underlying these processes at unprecedented resolution. Furthermore, the identification of molecular events that can serve as barcodes now facilitate the tracking of the trajectories of malignant and of immune cell populations over time within primary human samples, as these permit unambiguous identification of the clonal lineage of cell populations within heterogeneous phenotypes. Read More

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January 2022

Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging.

Nat Commun 2022 Jan 19;13(1):393. Epub 2022 Jan 19.

Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.

The fate of hematopoietic stem cells (HSCs) can be directed by microenvironmental factors including extracellular calcium ion concentration ([Ca]), but the local [Ca] around individual HSCs in vivo remains unknown. Here we develop intravital ratiometric analyses to quantify the absolute pH and [Ca] in the mouse calvarial bone marrow, taking into account the pH sensitivity of the calcium probe and the wavelength-dependent optical loss through bone. Unexpectedly, the mean [Ca] in the bone marrow (1. Read More

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January 2022

Loss of Ribosomal Protein Paralog Rpl22-like1 Blocks Lymphoid Development without Affecting Protein Synthesis.

J Immunol 2022 Jan 19. Epub 2022 Jan 19.

Blood Cell Development and Function Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111; and

Ribosomal proteins are thought to primarily facilitate biogenesis of the ribosome and its ability to synthesize protein. However, in this study, we show that Rpl22-like1 (Rpl22l1) regulates hematopoiesis without affecting ribosome biogenesis or bulk protein synthesis. Conditional loss of murine Rpl22l1 using stage or lineage-restricted Cre drivers impairs development of several hematopoietic lineages. Read More

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January 2022

Serological response following anti-SARS-CoV-2 vaccination in hematopoietic stem cell transplantation patients depends upon time from transplant, type of transplant and "booster" dose.

Haematologica 2022 Jan 20. Epub 2022 Jan 20.

Hematology and Stem Cell Transplantation Unit, AOUC Policlinico, Bari Italy; Department of Emergency and Organ Transplantation, "Aldo Moro"University School of Medicine, Bari, Italy.

Not available. Read More

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January 2022

[Advances in the diagnosis and management of sinusoidal obstruction syndrome after allogeneic hematopoietic stem cell transplantation].

Authors:
X Cai X H Zhang

Zhonghua Xue Ye Xue Za Zhi 2021 Dec;42(12):1052-1056

Department of Hematology, Peking University People's Hospital, Peking University Institute of Hematology, Beijing 100044, China.

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December 2021

[Impact of blood count recovery before haploidentical stem cell transplantation on outcomes in acute myeloid leukemia].

Zhonghua Xue Ye Xue Za Zhi 2021 Dec;42(12):1030-1034

Peking University People' s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, China Hematology Collaborative Innovation Center, Peking University, Beijing 100044, China.

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December 2021

[Analysis of risk factors of relapse after allogeneic hematopoietic stem cell transplantation in patients with t (8;21) acute myeloid leukemia].

Zhonghua Xue Ye Xue Za Zhi 2021 Dec;42(12):998-1004

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.

To investigate the risk factors of relapse after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients with t (8;21) acute myeloid leukemia (AML) . The clinical features of patients with t (8;21) AML who received allo-HSCT between January 2008 and October 2020 in the Hospital of Blood Disease and the Chinese Academy of Medical Sciences were retrospectively analyzed. Univariate and multivariate analyses were performed on the factors that might influence relapse. Read More

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December 2021

[The diagnostic value of whole blood Epstein-Barr virus DNA load in lymphoproliferative diseases after allogeneic hematopoietic stem cell transplantation].

Zhonghua Xue Ye Xue Za Zhi 2021 Nov;42(11):904-910

Department of Hematology, Peking University First Hospital, Beijing 100034, China.

To investigate the diagnostic value of whole blood quantitative PCR for DNA load of Epstein-Barr virus (EBV) in post-transplant lymphoproliferative disease (PTLD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) . A total of 694 patients with hematologic diseases who underwent allo-HSCT at the Hematology Department of Peking University First Hospital from April 2004 to April 2019 were included, and their data were retrospectively analyzed. ①Among the 694 cases, 29 cases (22 males and 7 females, with a median age of 22 (1-52) years) developed PTLD after allo-HSCT with a cumulative incidence of 4. Read More

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November 2021

Isolation of the side population from neurogenic niches enriches for endothelial cells.

PLoS One 2022 19;17(1):e0250752. Epub 2022 Jan 19.

Department of Cellular and Molecular Medicine, Neuroscience Program, Ottawa Hospital Research Institute, Brain and Mind Research Institute, University of Ottawa, Ottawa, Canada.

In stem cell research, DNA-binding dyes offer the ability to purify live stem cells using flow cytometry as they form a low-fluorescence side population due to the activity of ABC transporters. Adult neural stem cells exist within the lateral ventricle and dentate gyrus of the adult brain yet the ability of DNA-binding dyes to identify these adult stem cells as side populations remains untested. The following experiments utilize the efflux of a DNA-binding dye, Vyrbant DyeCycle Violet (DCV), to isolate bona fide side populations in the mouse dentate gyrus and subventricular zone (SVZ), and test their sensitivity to ABC transporter inhibitors. Read More

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January 2022

Disrupting N-glycan expression on tumor cells boosts chimeric antigen receptor T cell efficacy against solid malignancies.

Sci Transl Med 2022 Jan 19;14(628):eabg3072. Epub 2022 Jan 19.

Innovative Immunotherapies Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.

Immunotherapy with chimeric antigen receptor (CAR)-engineered T cells showed exceptional successes in patients with refractory B cell malignancies. However, first-in-human studies in solid tumors revealed unique hurdles contributing to poor demonstration of efficacy. Understanding the determinants of tumor recognition by CAR T cells should translate into the design of strategies that can overcome resistance. Read More

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January 2022

Pediatric patients with acute lymphoblastic leukemia treated with blinatumomab in a real-world setting: Results from the NEUF study.

Pediatr Blood Cancer 2022 Jan 19:e29562. Epub 2022 Jan 19.

Hôpital Universitaire Robert Debré, Paris, France.

Background: Prior to regulatory approval of blinatumomab in pediatric patients with relapsed/refractory Philadelphia chromosome-negative B-cell precursor acute lymphoblastic leukemia (R/R Ph- BCP-ALL), blinatumomab was made available via an expanded access program (EAP).

Procedure: This retrospective observational study included patients receiving blinatumomab in the EAP between January 1, 2014 and June 30, 2017 who were followed until death, entry into a clinical trial, end of follow-up, or end of the study period (December 31, 2017), whichever occurred first.

Results: Among 113 children enrolled, 72 were diagnosed with R/R Ph- BCP-ALL and 41 were minimal residual disease positive (MRD+, either Ph- or Ph+). Read More

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January 2022

Obstetrical and Perinatal Outcomes in Female Survivors of Childhood and Adolescent Cancer: A Population-Based Cohort Study.

J Natl Cancer Inst 2022 Jan 19. Epub 2022 Jan 19.

The Hospital for Sick Children, Division of Haematology/Oncology, Toronto, Ontario, Canada.

Background: The likelihood of pregnancy and risk of obstetrical or perinatal complications is inadequately documented in female survivors of pediatric cancer.

Methods: We assembled a population-based cohort of female survivors of cancer diagnosed at < 21 years in Ontario, Canada between 1985-2012. Survivors were matched 1:5 to women without prior cancer. Read More

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January 2022

MyD88-dependent TLR signaling oppositely regulates hematopoietic progenitor and stem cell formation in the embryo.

Development 2022 Jan 19. Epub 2022 Jan 19.

Abramson Family Cancer Research Institute and Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Hemogenic endothelial (HE) cells in the dorsal aorta undergo an endothelial to hematopoietic transition (EHT) to form multipotent progenitors, lympho-myeloid biased progenitors (LMPs), pre-hematopoietic stem cells (pre-HSCs) and adult-repopulating HSCs. These briefly accumulate in intra-arterial hematopoietic clusters (IAHCs) before being released into the circulation. It is generally assumed that the number of IAHC cells correlates with the number of HSCs. Read More

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January 2022

Cytogenetic guided therapy using blinatumomab and inotuzumab ozogamicin in a patient with relapse/refractory acute lymphoblastic leukemia.

J Oncol Pharm Pract 2022 Jan 19:10781552211073958. Epub 2022 Jan 19.

10605Florida Cancer Specialists & Research Institute LLC, Fort Myers, Florida, United States.

Introduction: Acute Lymphoblastic Leukemia (ALL) is an aggressive cancer that requires intense chemotherapy and has a high rate of recurrence. Treatments of Relapse/Refractory (R/R) B-cell ALL are limited. Blinatumomab, a bispecific T-cell engager (CD19/CD3) monocolonal antibody, and Inotuzumab Ozogamicin, an anti-CD22 antibody conjugate, are current recommended options. Read More

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January 2022

Time to initiation of pre-emptive therapy for cytomegalovirus impacts overall survival in pediatric hematopoietic stem cell transplant recipients.

Cytotherapy 2022 Jan 15. Epub 2022 Jan 15.

Stem Cell Transplantation and Cellular Therapies, MSK Kids, Memorial Sloan Kettering Cancer Center, New York, New York, USA. Electronic address:

Background Aims: Cytomegalovirus (CMV) reactivation is a significant complication following allogeneic hematopoietic stem cell transplant (HSCT) and affects upwards of 40% of pediatric HSCT patients. Pre-emptive therapy remains the only effective treatment strategy available for pediatric patients following CMV reactivation. Little is known about how the timing of induction treatment following CMV reactivation impacts outcomes in pediatric patients, especially following ex vivo T-cell-depleted (TCD) HSCT. Read More

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January 2022

Patient-specific MDS-RS iPSCs define the mis-spliced transcript repertoire and chromatin landscape of SF3B1-mutant HSPCs.

Blood Adv 2022 Jan 18. Epub 2022 Jan 18.

Icahn School of Medicine at Mount Sinai, New York, New York, United States.

SF3B1K700E is the most frequent mutation in myelodysplastic syndrome (MDS), but the mechanisms by which it drives MDS pathogenesis remain unclear. We derived a panel of 18 genetically matched SF3B1K700E and SF3B1WT induced pluripotent stem cell (iPSC) lines from patients with MDS with ring sideroblasts (MDS-RS) harboring isolated SF3B1K700E mutations and performed RNA- and ATAC- sequencing in purified CD34+/CD45+ hematopoietic stem/progenitor cells (HSPCs) derived from them. We developed a novel computational framework integrating splicing with transcript usage and gene expression analyses and derived a SF3B1K700E splicing signature consisting of 59 splicing events linked to 34 genes, which associates with the SF3B1 mutational status of primary MDS patient cells. Read More

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January 2022