31,511 results match your criteria heinrich

Filling the knowledge gap: A suspect screening study for 1310 potentially persistent and mobile chemicals with SFC- and HILIC-HRMS in two German river systems.

Water Res 2021 Sep 10;204:117645. Epub 2021 Sep 10.

Department of Analytical Chemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig 04318, Germany; University of Leipzig, Institute for Analytical Chemistry, Linnéstrasse 3, Leipzig 04103, Germany. Electronic address:

Persistent and mobile chemicals (PM chemicals) were searched for in surface waters by hydrophilic interaction liquid chromatography (HILIC) and supercritical fluid chromatography (SFC), both coupled to high resolution mass spectrometry (HRMS). A suspect screening was performed using a newly compiled list of 1310 potential PM chemicals to the data of 11 surface water samples from two river systems. In total, 64 compounds were identified by this approach. Read More

View Article and Full-Text PDF
September 2021

Screening, diagnosis and management of diabetic sensorimotor polyneuropathy in clinical practice: International expert consensus recommendations.

Diabetes Res Clin Pract 2021 Sep 18:109063. Epub 2021 Sep 18.

Faculty of Biology, Medicine and Health, University of Manchester and Manchester University Foundation Trust, Manchester, UK.

Diabetic sensorimotor polyneuropathy (DSPN) affects around one third of people with diabetes and accounts for considerable morbidity, increased risk of mortality, reduced quality of life, and increased health care costs resulting particularly from neuropathic pain and foot ulcers. Painful DSPN is encountered in 13-26% of diabetes patients, while up to 50% of patients with DSPN may be asymptomatic. Unfortunately, DSPN still remains inadequately diagnosed and treated. Read More

View Article and Full-Text PDF
September 2021

Effects of the Probiotic Activity of DSM 29784 in Cultures and Feeding Stuff.

J Agric Food Chem 2021 Sep 21. Epub 2021 Sep 21.

Institute of Nutritional Science, Chair of Food Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, Giessen 35392, Germany.

The European Union banned the usage of antibiotic growth promoters in animal production. The probiotic microorganism of the genus appeared to be an attractive candidate to replace antibiotics. The DSM 29784 is one of these strains. Read More

View Article and Full-Text PDF
September 2021

[Dementia Care Nurse - Feasibility study of outreach assistance for people with dementia and their family carers].

Pflege 2021 ;34(5):275-284

Institut für Gesundheits- und Pflegewissenschaft, Medizinische Fakultät, Martin-Luther-Universität Halle-Wittenberg.

Dementia Care Nurse - Feasibility study of outreach assistance for people with dementia and their family carers Case management for dementia is stipulated in the German national dementia strategy. The effectiveness of case management has been investigated many times, yet the results are heterogeneous. On the contrary, the implementation processes of case management concepts have to date rarely been described or evaluated in detail. Read More

View Article and Full-Text PDF
January 2021

Mapping Orbital-Resolved Magnetism in Single Lanthanide Atoms.

ACS Nano 2021 Sep 21. Epub 2021 Sep 21.

Center for Quantum Nanoscience, Institute for Basic Science (IBS), Seoul 03760, Republic of Korea.

Single lanthanide atoms and molecules are promising candidates for atomic data storage and quantum logic due to the long lifetime of their magnetic quantum states. Accessing and controlling these states through electrical transport requires precise knowledge of their electronic configuration at the level of individual atomic orbitals, especially of the outer shells involved in transport. However, no experimental techniques have so far shown the required sensitivity to probe single atoms with orbital selectivity. Read More

View Article and Full-Text PDF
September 2021

Does timing matter in radiotherapy of hepatocellular carcinoma? An experimental study in mice.

Cancer Med 2021 Sep 20. Epub 2021 Sep 20.

Institute of Anatomy II, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany.

This study investigates whether a chronotherapeutic treatment of hepatocellular carcinoma (HCC) may improve treatment efficacy and mitigate side effects on non-tumoral liver (NTL). HCC was induced in Per2::luc mice which were irradiated at four time points of the day. Proliferation and DNA-double strand breaks were analyzed in irradiated and nonirradiated animals by detection of Ki67 and γ-H2AX. Read More

View Article and Full-Text PDF
September 2021

Isolation of Plant Mitochondria Using Affinity Purification.

Methods Mol Biol 2022 ;2363:25-37

Institute of Plant Biochemistry, Cluster of Excellence on Plant Science (CEPLAS), Heinrich Heine University, Düsseldorf, Germany.

Mitochondria are highly dynamic organelles that are vital for eukaryotic cellular metabolism. Mitochondria account only for a small fraction of the total cell volume. As such, intact organelle isolation is a basic requirement to study mitochondrial biochemistry and physiology. Read More

View Article and Full-Text PDF
January 2022

Biallelic variants in YRDC cause a developmental disorder with progeroid features.

Hum Genet 2021 Sep 20. Epub 2021 Sep 20.

Institute of Human Genetics, University Medical Center Göttingen, Heinrich-Düker-Weg 12, 37073, Göttingen, Germany.

The highly conserved YrdC domain-containing protein (YRDC) interacts with the well-described KEOPS complex, regulating specific tRNA modifications to ensure accurate protein synthesis. Previous studies have linked the KEOPS complex to a role in promoting telomere maintenance and controlling genome integrity. Here, we report on a newborn with a severe neonatal progeroid phenotype including generalized loss of subcutaneous fat, microcephaly, growth retardation, wrinkled skin, renal failure, and premature death at the age of 12 days. Read More

View Article and Full-Text PDF
September 2021

Two adjacent C-terminal mutations enable expression of aryl-alcohol oxidase from Pleurotus eryngii in Pichia pastoris.

Appl Microbiol Biotechnol 2021 Sep 21. Epub 2021 Sep 21.

Institute of Biochemistry, Heinrich-Heine-University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany.

Fungal aryl-alcohol oxidases (AAOs) are attractive biocatalysts because they selectively oxidize a broad range of aromatic and aliphatic allylic primary alcohols while yielding hydrogen peroxide as the only by-product. However, their use is hampered by challenging and often unsuccessful heterologous expression. Production of PeAAO1 from Pleurotus eryngii ATCC 90787 in Pichia pastoris failed, while PeAAO2 from P. Read More

View Article and Full-Text PDF
September 2021

Early and Late Neural Correlates of Mentalizing: ALE Meta-Analyses in Adults, Children and Adolescents.

Soc Cogn Affect Neurosci 2021 Sep 21. Epub 2021 Sep 21.

Jacobs Center for Productive Youth Development, University of Zurich, Zurich, Switzerland.

The ability to understand mental states of others is referred to as mentalizing and enabled by our Theory of Mind. This social skill relies on brain regions comprising the mentalizing network, as robustly observed in adults, but also in a growing number of developmental studies. We summarized and compared neuroimaging evidence in children/adolescents and adults during mentalizing using coordinate-based activation likelihood estimation meta-analyses to inform about brain regions consistently or differentially engaged across age categories. Read More

View Article and Full-Text PDF
September 2021

Multimodal electrophysiological analyses reveal that reduced synaptic excitatory neurotransmission underlies seizures in a model of NMDAR antibody-mediated encephalitis.

Commun Biol 2021 Sep 20;4(1):1106. Epub 2021 Sep 20.

Institute of Health and Neurodevelopment, College of Health and Life Sciences, Aston University, Birmingham, UK.

Seizures are a prominent feature in N-Methyl-D-Aspartate receptor antibody (NMDAR antibody) encephalitis, a distinct neuro-immunological disorder in which specific human autoantibodies bind and crosslink the surface of NMDAR proteins thereby causing internalization and a state of NMDAR hypofunction. To further understand ictogenesis in this disorder, and to test a potential treatment compound, we developed an NMDAR antibody mediated rat seizure model that displays spontaneous epileptiform activity in vivo and in vitro. Using a combination of electrophysiological and dynamic causal modelling techniques we show that, contrary to expectation, reduction of synaptic excitatory, but not inhibitory, neurotransmission underlies the ictal events through alterations in the dynamical behaviour of microcircuits in brain tissue. Read More

View Article and Full-Text PDF
September 2021

Amyloid-β peptide dimers undergo a random coil to β-sheet transition in the aqueous phase but not at the neuronal membrane.

Proc Natl Acad Sci U S A 2021 Sep;118(39)

Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich, 52425 Jülich, Germany;

Mounting evidence suggests that the neuronal cell membrane is the main site of oligomer-mediated neuronal toxicity of amyloid-β peptides in Alzheimer's disease. To gain a detailed understanding of the mutual interference of amyloid-β oligomers and the neuronal membrane, we carried out microseconds of all-atom molecular dynamics (MD) simulations on the dimerization of amyloid-β (Aβ)42 in the aqueous phase and in the presence of a lipid bilayer mimicking the in vivo composition of neuronal membranes. The dimerization in solution is characterized by a random coil to β-sheet transition that seems on pathway to amyloid aggregation, while the interactions with the neuronal membrane decrease the order of the Aβ42 dimer by attenuating its propensity to form a β-sheet structure. Read More

View Article and Full-Text PDF
September 2021

Synthesis of (-)-deoxypodophyllotoxin and (-)-epipodophyllotoxin via a multi-enzyme cascade in E. coli.

Microb Cell Fact 2021 Sep 20;20(1):183. Epub 2021 Sep 20.

Institute of Biochemistry, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany.

Background: The aryltetralin lignan (-)-podophyllotoxin is a potent antiviral and anti-neoplastic compound that is mainly found in Podophyllum plant species. Over the years, the commercial demand for this compound rose notably because of the high clinical importance of its semi-synthetic chemotherapeutic derivatives etoposide and teniposide. To satisfy this demand, (-)-podophyllotoxin is conventionally isolated from the roots and rhizomes of Sinopodophyllum hexandrum, which can only grow in few regions and is now endangered by overexploitation and environmental damage. Read More

View Article and Full-Text PDF
September 2021

Can TSH level and premenstrual spotting constitute a non-invasive marker for the diagnosis of endometriosis?

BMC Womens Health 2021 Sep 20;21(1):336. Epub 2021 Sep 20.

Department of OB/GYN and REI (UniKiD), Medical Center, Medical Faculty, University of Düsseldorf, Moorenstrasse 5, 40225, Düsseldorf, Germany.

Background: To date, there is no reliable non-invasive marker for the early detection and diagnosis of endometriosis available possibly resulting in a delayed diagnosis and consequently an unnecessary long ordeal for the individual woman. Therefore, the primary objective of the current study was to evaluate whether the combination of a thyroid-stimulating hormone (TSH) level > 2.5 µlU/ml and premenstrual spotting could serve as non-invasive markers of endometriosis. Read More

View Article and Full-Text PDF
September 2021

Pool water disinfection by ozone-bromine treatment: Assessing the disinfectant efficacy and the occurrence and in vitro toxicity of brominated disinfection by-products.

Water Res 2021 Sep 9;204:117648. Epub 2021 Sep 9.

German Environment Agency (UBA), Section Water Treatment, Schichauweg 58, Berlin 12307, Germany. Electronic address:

Pool water is continuously circulated and reused after an extensive treatment including disinfection by chlorination, ozonation or UV treatment. In Germany, these methods are regulated by DIN standard 19643. Recently, the DIN standard has been extended by a new disinfection method using hypobromous acid as disinfectant formed by introducing ozone into water with naturally or artificially high bromide content during water treatment. Read More

View Article and Full-Text PDF
September 2021

Advanced brain ageing in Parkinson's disease is related to disease duration and individual impairment.

Brain Commun 2021 23;3(3):fcab191. Epub 2021 Aug 23.

Institute of Neuroscience and Medicine (INM-1, INM-7, INM-11), Jülich, Germany.

Machine learning can reliably predict individual age from MRI data, revealing that patients with neurodegenerative disorders show an elevated biological age. A surprising gap in the literature, however, pertains to Parkinson's disease. Here, we evaluate brain age in two cohorts of Parkinson's patients and investigated the relationship between individual brain age and clinical characteristics. Read More

View Article and Full-Text PDF

Generation of Targeted Knockout Mutants in Using CRISPR/Cas9.

Bio Protoc 2017 Jul 5;7(13):e2384. Epub 2017 Jul 5.

Institute of Plant Biochemistry, Cluster of Excellence on Plant Science (CEPLAS), Center for Synthetic Life Sciences (CSL), Heinrich Heine University, Düsseldorf, Germany.

The CRISPR/Cas9 system has emerged as a powerful tool for gene editing in plants and beyond. We have developed a plant vector system for targeted Cas9-dependent mutagenesis of genes in up to two different target sites in . This protocol describes a simple 1-week cloning procedure for a single T-DNA vector containing the genes for Cas9 and sgRNAs, as well as the detection of induced mutations . Read More

View Article and Full-Text PDF

Whole-seed Immunolabeling of Mucilage Polysaccharides.

Bio Protoc 2017 Jun 5;7(11):e2323. Epub 2017 Jun 5.

Institute for Bio- and Geosciences (IBG-2: Plant Sciences), Forschungszentrum Jülich, Jülich, Germany.

In addition to synthesizing and secreting copious amounts of pectic polymers ( Young , 2008 ), seed coat epidermal cells produce small amounts of cellulose and hemicelluloses typical of secondary cell walls ( Voiniciuc , 2015c ). These components are intricately linked and are released as a large mucilage capsule upon hydration of mature seeds. Alterations in the structure of minor mucilage components can have dramatic effects on the architecture of this gelatinous cell wall. Read More

View Article and Full-Text PDF

Wood Anatomy of Douglas-Fir in Eastern Arizona and Its Relationship With Pacific Basin Climate.

Front Plant Sci 2021 1;12:702442. Epub 2021 Sep 1.

GFZ German Research Centre for Geosciences, Potsdam, Germany.

Dendroclimatic reconstructions, which are a well-known tool for extending records of climatic variability, have recently been expanded by using wood anatomical parameters. However, the relationships between wood cellular structures and large-scale climatic patterns, such as El Niño-Southern Oscillation (ENSO) and the Pacific Decadal Oscillation (PDO), are still not completely understood, hindering the potential for wood anatomy as a paleoclimatic proxy. To better understand the teleconnection between regional and local climate processes in the western United States, our main objective was to assess the value of these emerging tree-ring parameters for reconstructing climate dynamics. Read More

View Article and Full-Text PDF
September 2021

Paradigmatic Relations Interact During the Production of Complex Words: Evidence From Variable Plurals in Dutch.

Front Psychol 2021 1;12:720017. Epub 2021 Sep 1.

Centre for Language Studies, Radboud University, Nijmegen, Netherlands.

A growing body of work in psycholinguistics suggests that morphological relations between word forms affect the processing of complex words. Previous studies have usually focused on a particular type of paradigmatic relation, for example the relation between paradigm members, or the relation between alternative forms filling a particular paradigm cell. However, potential interactions between different types of paradigmatic relations have remained relatively unexplored. Read More

View Article and Full-Text PDF
September 2021

Identification of Phonology-Related Genes and Functional Characterization of Broca's and Wernicke's Regions in Language and Learning Disorders.

Front Neurosci 2021 3;15:680762. Epub 2021 Sep 3.

Cécile and Oskar Vogt Institute for Brain Research, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.

Impaired phonological processing is a leading symptom of multifactorial language and learning disorders suggesting a common biological basis. Here we evaluated studies of dyslexia, dyscalculia, specific language impairment (SLI), and the logopenic variant of primary progressive aphasia (lvPPA) seeking for shared risk genes in Broca's and Wernicke's regions, being key for phonological processing within the complex language network. The identified "phonology-related genes" from literature were functionally characterized using Atlas-based expression mapping (JuGEx) and gene set enrichment. Read More

View Article and Full-Text PDF
September 2021

High-performance thin-layer chromatography combined with effect-directed assays and high-resolution mass spectrometry as an emerging hyphenated technology: A tutorial review.

Anal Chim Acta 2021 Oct 18;1180:338644. Epub 2021 May 18.

Chair of Food Science, Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address:

Among the analytical advances, hyphenated HPTLC offers great potential for solving pressing questions. It provides straightforward information about effects arising from individual compounds in complex or natural samples separated in parallel. The chromatographic separation is combined with effect-directed detection using enzymatic or biological assays. Read More

View Article and Full-Text PDF
October 2021

Corrigendum to "Wind and fairness in ski jumping: A computer modelling analysis" [J. Biomech. 75 (2018) 147-153].

J Biomech 2021 Sep 15;128:110690. Epub 2021 Sep 15.

Aachen University of Applied Sciences, Institute of Bioengineering, Heinrich-Mußmann-Str. 1, 52428 Jülich, Germany.

View Article and Full-Text PDF
September 2021

The cognitive anxiety sensitivity treatment (CAST) in anxiety prevention - Focus on separation anxiety and interoception.

Eur Neuropsychopharmacol 2021 Sep 15;53:104-113. Epub 2021 Sep 15.

Department of Psychiatry and Psychotherapy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany; Center for Basics in Neuromodulation, Faculty of Medicine, University of Freiburg, Freiburg, Germany. Electronic address:

Given the high prevalence and considerable clinical and societal burden of anxiety disorders, preventive measures are urgently warranted to reduce their incidence and overall healthcare impact. Anxiety sensitivity (AS) - a key element in learning theories of anxiety disorders in the context of interoceptive conditioning - constitutes a malleable risk factor of particularly panic disorder and separation anxiety, which share developmental, nosological, epidemiological and pathomechanistic characteristics. The computer-assisted 'Cognitive Anxiety Sensitivity Treatment' (CAST) targeting interoceptive anxiety symptoms (cf. Read More

View Article and Full-Text PDF
September 2021

A qPCR-based method for molecular subtype classification of urinary bladder cancer - stromal gene expressions show higher prognostic values than intrinsic tumor genes.

Int J Cancer 2021 Sep 18. Epub 2021 Sep 18.

Department of Urology, University of Duisburg-Essen, Essen, Germany.

Transcriptome-based molecular subtypes of muscle-invasive bladder cancer (MIBC) have been shown to be both prognostic and predictive, but are not used in routine clinical practice. We aimed to develop a feasible, RT-qPCR-based method for molecular subtyping. First, we defined a 68-gene set covering tumor intrinsic (luminal, basal, squamous, neuronal, epithelial-to-mesenchymal, in situ carcinoma) and stromal (immune, extracellular matrix, p53-like) signatures. Read More

View Article and Full-Text PDF
September 2021

Cross-sectional study on exhaled nitric oxide in relation to upper airway inflammatory disorders with regard to asthma and perennial sensitisation.

Clin Exp Allergy 2021 Sep 18. Epub 2021 Sep 18.

Department of Medical Sciences, Clinical Physiology, Uppsala University, Uppsala, Sweden.

Background: Fractional exhaled nitric oxide (FeNO) is a well-known marker of type-2 inflammation. FeNO is elevated in asthma and allergic rhinitis, with IgE sensitisation as a major determinant.

Objective: We aimed to see if there was an independent association between upper airway inflammatory disorders (UAID) and FeNO, after adjustment for asthma and sensitisation, in a multi-centre population-based study. Read More

View Article and Full-Text PDF
September 2021

Interpretation of electrophysiological responses and generalization of findings requires knowledge of physical stimulus characteristics.

Sven P Heinrich

Doc Ophthalmol 2021 Sep 18. Epub 2021 Sep 18.

Eye Center, Medical Center, University of Freiburg, Killianstr. 5, 79106, Freiburg, Germany.

View Article and Full-Text PDF
September 2021