326,252 results match your criteria healthy subjects

Intradermal lipopolysaccharide challenge as an acute in vivo inflammatory model in healthy volunteers.

Br J Clin Pharmacol 2021 Jul 22. Epub 2021 Jul 22.

Centre for Human Drug Research, Leiden, the Netherlands.

Introduction: Whereas intravenous administration of TLR4 ligand lipopolysaccharide (LPS) to human volunteers is frequently used in clinical pharmacology studies, systemic use of LPS has practical limitations. We aimed to characterize the intradermal LPS response in healthy volunteers, and as such qualify the method as local inflammation model for clinical pharmacology studies.

Methods: 18 healthy male volunteers received two or four intradermal 10 ng LPS injections and one saline injection on the forearms. Read More

View Article and Full-Text PDF

Neurotransmitters and Neuropeptides decrease PD-1 in T cells of healthy subjects and patients with hepatocellular carcinoma (HCC), and increase their proliferation and eradication of HCC cells.

Neuropeptides 2021 May 12;89:102159. Epub 2021 May 12.

Institute of Gene Therapy, Hadassah Hebrew University Hospital, Ein Karem, Jerusalem 91120, Israel.

T cells of aged people, and of patients with either cancer or severe infections (including COVID-19), are often exhausted, senescent and dysfunctional, leading to increased susceptibilities, complications and mortality. Neurotransmitters and Neuropeptides bind their receptors in T cells, and induce multiple beneficial T cell functions. Yet, T cells of different people vary in the expression levels of Neurotransmitter and Neuropeptide receptors, and in the magnitude of the corresponding effects. Read More

View Article and Full-Text PDF

Spinous process screw fixation: A salvage technique in sub-axial cervical spinal instrumentation.

World Neurosurg 2021 Jul 19. Epub 2021 Jul 19.

Department of Orthopaedics, Peking University Third Hospital, Beijing, China. Electronic address:

Objective: To evaluate the feasibility and efficacy of spinous process screws (SPS) in sub-axial cervical fixation.

Methods: A retrospective study was conducted on seven patients receiving posterior cervical instrumentation for sub-axial fracture dislocation or atlantoaxial dislocation from 2014 to 2015. Additionally, a biomechanical test was performed on seven fresh-frozen cadaveric spine samples (from the occiput to C7) from healthy human subjects with no history of spinal trauma. Read More

View Article and Full-Text PDF

Agreement between Compass Fundus Perimeter New Grid and 10-2 Testing Protocols for Detecting Central Visual Field Defects.

Ophthalmol Glaucoma 2021 Jul 19. Epub 2021 Jul 19.

Hamilton Glaucoma Center, Shiley Eye Institute, and the Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, CA, United States. Electronic address:

Purpose: To evaluate the agreement between Compass New Grid (NG) and 10-2 test protocols for detecting early glaucomatous defects in the central 10 degrees of the visual field (CVFD).

Design: Cross-sectional study.

Subjects And Controls: A total of 123 eyes of 14 healthy individuals, 17 glaucoma suspects and 32 glaucoma patients were enrolled. Read More

View Article and Full-Text PDF

Perinatal SSRI exposure disrupts G protein-coupled receptor BAI3 in developing dentate gyrus and adult emotional behavior: relevance to psychiatric disorders.

Neuroscience 2021 Jul 19. Epub 2021 Jul 19.

School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA. Electronic address:

Selective serotonin reuptake inhibitor (SSRI) antidepressants are widely prescribed to pregnant women suffering with depression, although the long-term impact of these medications on exposed offspring are poorly understood. Perinatal SSRI exposure alters human offspring's neurodevelopment and increases risk for psychiatric illness in later life. Rodent studies suggest that perinatal SSRI-induced behavioral abnormalities are driven by changes in the serotonin system as well as epigenetic and transcriptomic changes in the developing hippocampus. Read More

View Article and Full-Text PDF

Anti-microbial antibody response is associated with future onset of Crohn's disease independent of biomarkers of altered gut barrier function, subclinical inflammation, and genetic risk.

Gastroenterology 2021 Jul 19. Epub 2021 Jul 19.

Zane Cohen Centre for Digestive Diseases, Mount Sinai Hospital, Toronto, Ontario, Canada; Division of Gastroenterology & Hepatology, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. Electronic address:

Background: /Aim: Altered host immune reactivity to microbial antigens is hypothesized to trigger the onset of Crohn's disease (CD). We aimed to assess whether increased serum anti-microbial antibody response in asymptomatic first-degree relatives (FDR) of CD patients is an independent risk factor for future CD development.

Methods: We measured host serum antibody response to six microbial antigens at enrollment (Prometheus ELISA test: ASCA IgA/IgG, anti-OmpC, anti-A4-Fla2, anti-FlaX, anti-CBir1) and derived the sum of positive antibodies (AS). Read More

View Article and Full-Text PDF

Individual variability in human urinary metabolites identifies age-related, body mass index-related, and sex-related biomarkers.

Mol Genet Genomic Med 2021 Jul 22:e1738. Epub 2021 Jul 22.

Materia Medica Development Group, Institute of Medicinal Chemistry, Lanzhou University School of Pharmacy, Lanzhou, China.

Background: Metabolites present in human urine can be influenced by individual physiological parameters (e.g., body mass index [BMI], age, and sex). Read More

View Article and Full-Text PDF

Measuring the difference in natural head position between the standing and sitting positions using an inertial measurement unit.

J Oral Rehabil 2021 Jul 22. Epub 2021 Jul 22.

Division of Orthodontics, University Clinics of Dental Medicine, University of Geneva, Switzerland.

The purpose of this study was to compare the natural head position (NHP) in the sitting position to the NHP in a standing position using inertial measurement unit (IMU) and lateral photographs. Twenty healthy young adult volunteers were asked to look at a mirror located at 1 meter in front of their eyes while being recorded with the IMU system. Lateral photographs were also taken. Read More

View Article and Full-Text PDF

cVEMP and VAT for the diagnosis of vestibular migraine.

Eur J Clin Invest 2021 Jul 22:e13657. Epub 2021 Jul 22.

Department 4 of Neurology, Cangzhou Central Hospital, Cangzhou, 061000, Hebei, China.

Background: Although the diagnostic criteria of vestibular migraine (VM) have already been defined, various clinical manifestations of VM and the lack of pathognomonic biomarker result in high rate of misdiagnosis and mismanagement. A timely and accurate diagnosis tool for evaluation of VM is highly needed.

Objective: The current study aims to investigate the potential feasibility of cervical vestibular evoked myogenic potential (cVEMP) and vestibular autorotation test (VAT) as a diagnosis tool for VM. Read More

View Article and Full-Text PDF

Neither low salivary amylase activity, cooling cooked white rice, nor single nucleotide polymorphisms in starch-digesting enzymes reduce glycemic index or starch digestibility: a randomized, crossover trial in healthy adults.

Am J Clin Nutr 2021 Jul 22. Epub 2021 Jul 22.

INQUIS Clinical Research, Ltd (formerly GI Labs), Toronto, Ontario, Canada.

Background: It was suggested that low salivary-amylase activity (SAA) and cooling or stir-frying cooked starch decreases its digestibility and glycemic index.

Objective: We determined the effects of SAA, cooling, and single-nucleotide polymorphisms (SNPs) in the salivary amylase (AMY1), pancreatic amylase (AMY2A, AMY2B), maltase-glucoamylase (MGAM), and sucrase-isomaltase (SI) genes on starch digestibility and glycemic index of cooked polished rice.

Methods: Healthy subjects [pilot, n = 12; main, n = 20 with low-SAA (<50 U/mL), and n = 20 with high-SAA (>105 U/mL)] consumed test meals containing 25 g (pilot) or 50 g (main) available carbohydrate at a contract research organization using open-label (pilot) or assessor-blinded (main), randomized, crossover, Latin-square designs (trial registration: NCT03667963). Read More

View Article and Full-Text PDF

Asymptomatic morphometric vertebral fractures and its associated factors: A cross-sectional study among adults in a selected urban area in Selangor, Malaysia.

PLoS One 2021 22;16(7):e0255069. Epub 2021 Jul 22.

Puchong Specialist Centre, Puchong, Selangor, Malaysia.

Objective: This study aimed to determine the prevalence of vertebral fractures (VF) in a selected urban population in Malaysia and to explore possible variables associated with VF in the study population.

Methods: A cross-sectional study involving community-living, healthy subjects aged between 45-90 years from the state of Selangor, Malaysia, were invited to attend a bone health check-up. Subjects with diseases known to affect bone metabolism or were on treatment for osteoporosis (OP) were excluded. Read More

View Article and Full-Text PDF

Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.

PLoS One 2021 22;16(7):e0254921. Epub 2021 Jul 22.

Department of Genetics and Molecular Biology, Prof M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, Royapuram, Chennai, India.

Aim: Diabetic foot ulcer (DFU) is a major concern in diabetes and its control requires in-depth molecular investigation. The present study aimed to screen the expression of microRNA-210 (miR-210) and its association in hypoxic pathway in DFU patients.

Methods: The study consists of 3 groups of circulation samples (50 in each group of: healthy volunteers, T2DM and T2DM with DFU) and 2 groups of tissue samples (10 in each group of: control and T2DM with DFU). Read More

View Article and Full-Text PDF

Persistent homology as a new method of the assessment of heart rate variability.

PLoS One 2021 22;16(7):e0253851. Epub 2021 Jul 22.

Department of Hypertension and Diabetology, Medical University of Gdańsk, Gdańsk, Poland.

Heart rate variability (hrv) is a physiological phenomenon of the variation in the length of the time interval between consecutive heartbeats. In many cases it could be an indicator of the development of pathological states. The classical approach to the analysis of hrv includes time domain methods and frequency domain methods. Read More

View Article and Full-Text PDF

Overload of anxiety on postural control impairments in chronic stroke survivors: The role of external focus and cognitive task on the automaticity of postural control.

PLoS One 2021 22;16(7):e0252131. Epub 2021 Jul 22.

Department of Neurosciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

Background: Despite the high prevalence of anxiety among chronic stroke survivors and evidence of its negative effects on postural control in healthy subjects, it is unclear whether anxiety also affects postural control in these patients. Recent evidence of improved postural control of healthy subjects by distracting the attention using an external focus (EF) or cognitive task, raises the question of whether similar benefits would be observed in stroke survivors. Thus, the current study aimed to investigate the effects of anxiety and distracting the attention on postural control of chronic stroke survivors in terms of both postural sway measures and neuromuscular regulation. Read More

View Article and Full-Text PDF

Effects of diaphragmatic control on multiparametric analysis of the sniff nasal inspiratory pressure test and inspiratory muscle activity in healthy subjects.

PLoS One 2021 22;16(7):e0253132. Epub 2021 Jul 22.

PneumoCardioVascular Lab/HUOL, Hospital Universitário Onofre Lopes, Empresa Brasileira de Serviços Hospitalares and Departamento de Fisioterapia Universidade Federal do Rio Grande do Norte, Natal, Brazil.

Background: We investigated the influence of diaphragmatic activation control (diaphC) on the relaxation rate, contractile properties and electrical activity of the inspiratory muscles of healthy subjects. Assessments were performed non-invasively using the sniff inspiratory pressure test (SNIP) and surface electromyography, respectively.

Methods: Twenty-two subjects (10 men and 12 women) performed 10 sniff maneuvers in two different days: with and without diaphC instructions. Read More

View Article and Full-Text PDF

Population Pharmacokinetic Analysis of Ulotaront in Subjects with Schizophrenia.

CPT Pharmacometrics Syst Pharmacol 2021 Jul 22. Epub 2021 Jul 22.

Sunovion Pharmaceuticals Inc, Marlborough, MA, USA.

Ulotaront (SEP-363856) is a trace amine-associated receptor 1 (TAAR1) agonist with 5-HT agonist activity in Phase 3 development for the treatment of schizophrenia. The efficacy of ulotaront is not mediated by blockade of D or 5-HT receptors. The aim of this study was to evaluate the population PK of ulotaront in adult subjects using pooled data from seven Phase 1 studies, one Phase 2 acute study, and one 6-month extension study. Read More

View Article and Full-Text PDF

Evaluation of the Frequency of RETN c.62G>A and RETN c.-180C>G Polymorphisms in the Resistin Coding Gene in Girls with Anorexia Nervosa.

Endokrynol Pol 2021 Jul 22. Epub 2021 Jul 22.

Department of Adult Psychiatry, University of Medical Sciences in Poznań, Poland.

Introduction: Anorexia nervosa (AN) is a serious psychosomatic syndrome, classified as an eating disorder. AN patients strive to lose weight below normal limits defined for a specific age and height, achieving their goal even at the expense of extreme emaciation. AN has a multifactorial etiology. Read More

View Article and Full-Text PDF

Evaluation of the Frequency of ADIPOQ c.45 T>G and ADIPOQ c.276 G>T Polymorphisms in the Adiponectin Coding Gene in Girls with Anorexia Nervosa.

Endokrynol Pol 2021 Jul 22. Epub 2021 Jul 22.

Department of Adult Psychiatry, University of Medical Sciences in Poznań, Poland.

Introduction: Anorexia nervosa (AN) is a serious chronic psychosomatic disorder, the essence of which are attempts to obtain a slim silhouette by deliberate weight loss (restrictive diet, strenuous physical exercise, provoking vomiting). The etiology of this disorder is multifactorial. Genetic factors which influence the predisposition to AN have been searched for. Read More

View Article and Full-Text PDF

Preclinical and clinical study on [F]DRKXH1: a novel β-amyloid PET tracer for Alzheimer's disease.

Eur J Nucl Med Mol Imaging 2021 Jul 22. Epub 2021 Jul 22.

Department of Nuclear Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, No. 1665, Kongjiang Street, Yangpu District, Shanghai, 200092, China.

Background: The deposition of β-amyloid (Aβ) in the brain is a biomarker of Alzheimer's disease (AD). Highly sensitive Aβ positron emission tomography (PET) imaging plays an essential role in diagnosing and evaluating the therapeutic effects of AD.

Aim: To synthesize a new Aβ tracer [F]DRKXH1 (5-(4-(6-(2-[18]fluoroethoxy)ethoxy)imidazo[1,2-alpha]pyridin-2-yl)phenyl) and evaluate the tracer performance by biodistribution analysis, in vivo small-animal PET-CT dynamic scan, ex vivo and in vitro autoradiography, and PET in human subjects. Read More

View Article and Full-Text PDF

Index-based dietary patterns and stomach cancer in a Chinese population.

Eur J Cancer Prev 2021 Jul 20. Epub 2021 Jul 20.

Department of Epidemiology, Fielding School of Public Health, University of California at Los Angeles (UCLA), Los Angeles, California, USA Department of Non-communicable Chronic Disease Control, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu Province Department of Epidemiology, School of Public Health, Fudan University, Shanghai Ganyu County Center for Disease Control and Prevention, Ganyu Dafeng County Center for Disease Control and Prevention, Dafeng Chuzhou County Center for Disease Control and Prevention, Chuzhou Tongshan County Center for Disease Control and Prevention, Tongshan, Jiangsu Province, China Jonsson Comprehensive Cancer Center, UCLA School of Nursing, UCLA, Los Angeles, California, USA Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing, China Department of Social and Preventive Medicine, State University of New York at Buffalo, Buffalo, New York Center for Human Nutrition, Department of Medicine, UCLA David Geffen School of Medicine, University of California at Los Angeles (UCLA), Los Angeles, California, USA Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.

Objectives: Dietary factors are of importance in the development of stomach cancer. This study aims to examine index-based dietary patterns associated with stomach cancer in a Chinese population.

Methods: Using data from a population-based case-control study conducted in Jiangsu Province, China, we included a total of 8432 participants (1900 stomach cancer cases and 6532 controls). Read More

View Article and Full-Text PDF

Assessment of distinct subcortical and cortical contributions to affect and approach/withdrawal behavior by means of resting-state functional connectivity approach.

Behav Neurosci 2021 Jul 22. Epub 2021 Jul 22.

Department of Applied Physics.

The relevance of subcortical structures and interhemispheric subcortical-cortical interactions among positive/negative affect and approach/withdrawal tendencies during resting-state are not fully understood. Gaining this knowledge may foster the know-how on their role in subsequent task-engagement and also on the interlinkage among affective measures and approach/withdrawal dichotomy. Here we performed a study based on Region of Interest (ROI)-based analysis and graph-theory estimates for global and subnetworks on a limited sample of healthy 50 male volunteers who recorded resting functional magnetic resonance imaging (fMRI) and self-reported measures of Positive and Negative Affect Schedule (PANAS) and BAS-BIS (Behavioral Activation System-Behavioral Inhibition System) scores. Read More

View Article and Full-Text PDF

Magnetic Resonance Imaging-Based Structural Covariance Changes of the Striatum in Lifelong Premature Ejaculation Patients.

J Magn Reson Imaging 2021 Jul 22. Epub 2021 Jul 22.

Life Sciences Research Center, School of Life Science and Technology, Xidian University, Xi'an, China.

Background: The striatum has been reported to be implicated in various neurological diseases, including lifelong premature ejaculation (LPE). Altered striatum-related functional connectivity was investigated in LPE patients in previous studies; however, structural abnormalities in the striatum have been less studied in LPE.

Purpose: To identify the gray matter volume (GMV) and structural covariance patterns of the striatum between LPE patients and healthy controls (HCs). Read More

View Article and Full-Text PDF

Response of gene regulatory networks after infection of H3N2 virus.

J Bioinform Comput Biol 2021 Jul 21:2150017. Epub 2021 Jul 21.

Department of Systems Science, University of Shanghai for Science and Technology, Jungong Road, No. 516, Shanghai 200093, P. R. China.

Viral infection is a complicated dynamic process, in which viruses intrude into cells to duplicate themselves and trigger succeeding biological processes regulated by genes. It may lead to a serious disaster to human's health. A scheme is proposed to monitor the response of cells after being infected by viruses. Read More

View Article and Full-Text PDF

Volumetric coronary endothelial function assessment: a feasibility study exploiting stack-of-stars 3D cine MRI and image-based respiratory self-gating.

NMR Biomed 2021 Jul 21:e4589. Epub 2021 Jul 21.

Russel H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University, Baltimore, Maryland, USA.

Abnormal coronary endothelial function (CEF), manifesting as depressed vasoreactive responses to endothelial-specific stressors, occurs early in atherosclerosis, independently predicts cardiovascular events, and responds to cardioprotective interventions. CEF is spatially heterogeneous along a coronary artery in patients with atherosclerosis, and thus recently developed and tested non-invasive 2D MRI techniques to measure CEF may not capture the extent of changes in CEF in a given coronary artery. The purpose of this study was to develop and test the first volumetric coronary 3D MRI cine method for assessing CEF along the proximal and mid-coronary arteries with isotropic spatial resolution and in free-breathing. Read More

View Article and Full-Text PDF

Evaluation of migraine patients with optical coherence tomography angiography.

Int Ophthalmol 2021 Jul 22. Epub 2021 Jul 22.

Department of Ophthalmology, Mus State Hospital, Mus, Turkey.

Purpose: To compare optical coherence tomography angiography (OCTA) findings in cases with migraine and healthy controls.

Methods: Thirty-eight eyes of 19 patients with migraine with aura and 38 eyes of 19 healthy subjects were enrolled in this prospective and comparative study. All patients and healthy controls were evaluated with OCTA (Triton, Topcon®, Tokyo, Japan). Read More

View Article and Full-Text PDF

Study of DYRK1B gene expression and its association with metabolic syndrome in a small cohort of Egyptians.

Mol Biol Rep 2021 Jul 21. Epub 2021 Jul 21.

Human Genetics and Genome Research Division, Medical Molecular Genetics Department, National Research Centre, Giza, Egypt.

Background: A cluster of many risk factors for type 2 diabetes and cardiovascular disease is used to describe the metabolic syndrome (MetS). Moreover, genetic differences associated with metabolic syndrome play a key role in its prevalence and side effects. This study aims to investigate the expression of DYRK1B and its association with metabolic syndrome in a small cohort of Egyptian. Read More

View Article and Full-Text PDF

Non-invasive Clinical Measurement of Ocular Rigidity and Comparison to Biomechanical and Morphological Parameters in Glaucomatous and Healthy Subjects.

Front Med (Lausanne) 2021 5;8:701997. Epub 2021 Jul 5.

Department of Ophthalmology and Visual Sciences, The Ohio State University Wexner Medical Center, Columbus, OH, United States.

To assess ocular rigidity using dynamic optical coherence tomography (OCT) videos in glaucomatous and healthy subjects, and to evaluate how ocular rigidity correlates with biomechanical and morphological characteristics of the human eye. Ocular rigidity was calculated using Friedenwald's empirical equation which estimates the change in intraocular pressure (IOP) produced by volumetric changes of the eye due to choroidal pulsations with each heartbeat. High-speed OCT video was utilized to non-invasively measure changes in choroidal volume through time-series analysis. Read More

View Article and Full-Text PDF

Identifying and Characterizing Types of Balance Recovery Strategies Among Females and Males to Prevent Injuries in Free-Standing Public Transport Passengers.

Front Bioeng Biotechnol 2021 5;9:670498. Epub 2021 Jul 5.

Swedish National Road and Transport Research Institute, Linköping, Sweden.

Free-standing passengers on public transport are subjected to perturbations during non-collision incidents caused by driver maneuvers, increasing the risk of injury. In the literature, the step strategy is described as a recovery strategy during severe perturbations. However, stepping strategies increase body displacement, ultimately subjecting passengers to higher risk of impacts and falls on public transport. Read More

View Article and Full-Text PDF

Addressing Health Literacy in Schools in Germany: Concept Analysis of the Mandatory Digital and Media Literacy School Curriculum.

Front Public Health 2021 5;9:687389. Epub 2021 Jul 5.

Faculty of Educational Science, Interdisciplinary Center for Health Literacy Research, Bielefeld University, Bielefeld, Germany.

It is generally agreed upon that the development of health literacy should be addressed from an early age onwards in order to empower children to develop their full health potential. Schools can be seen as an ideal venue for strengthening health literacy because they reach almost all school-aged children throughout their school years. The development of health literacy at a young age is a catalyst for healthy development throughout across the life span. Read More

View Article and Full-Text PDF