4,278 results match your criteria harvested serum

The Early Diagnostic Efficacy of Serum Histone H3 in Rabbit Urosepsis Model.

Biomed Res Int 2021 16;2021:9969344. Epub 2021 Jul 16.

Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.

Objective: We want to explore the changing law of circulating histones in the acute stage of urosepsis and to find more sensitive and specific biomarkers for diagnosing urosepsis as early as possible.

Methods: Twenty healthy male New Zealand rabbits were randomly divided into 4 groups ( = 5): the control group, sham group, model group of LPS 600 g/kg, and model group of LPS 1000 g/kg. Heart rate (HR), respiration rate (RR), rectal temperature (T), and mean arterial pressure (MAP) were examined at 1, 3, 6, 12, and 24 hours after operation. Read More

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The Impacts of Surgery and Intracerebral Electrodes in C57BL/6J Mouse Kainate Model of Epileptogenesis: Seizure Threshold, Proteomics, and Cytokine Profiles.

Front Neurol 2021 12;12:625017. Epub 2021 Jul 12.

Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, United Kingdom.

Intracranial electroencephalography (EEG) is commonly used to study epileptogenesis and epilepsy in experimental models. Chronic gliosis and neurodegeneration at the injury site are known to be associated with surgically implanted electrodes in both humans and experimental models. Currently, however, there are no reports on the impact of intracerebral electrodes on proteins in the hippocampus and proinflammatory cytokines in the cerebral cortex and plasma in experimental models. Read More

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Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy.

Shi Sui Yang Hou

Scand Cardiovasc J 2021 Jul 23:1-10. Epub 2021 Jul 23.

Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, China.

Objective: The present study aimed to evaluate the protective role of cilengitide (CGT), an integrin αvβ3 and αvβ5 inhibitor, on doxorubicin (DOX)-induced myocardial fibrosis and cardiac dysfunction in a rat model. . Forty male rats were randomly divided into four groups: DOX ( = 12), intraperitoneal (i. Read More

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[Parathyroid hormone-related protein aggravates nonalcoholic fatty liver disease induced by methionine choline-deficient diet in mice].

B Qin N Diao L Bai

Nan Fang Yi Ke Da Xue Xue Bao 2021 Jul;41(7):1037-1043

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To study the effect of parathyroid hormone-related protein (PTHrP) on nonalcoholic fatty liver disease (NAFLD) induced by methionine choline-deficient diet (MCD) in mice.

Methods: Twelve male C57BL/6J mice were randomized into blank control group, vehicle group and PTHrP group (=4). The mice in vehicle group and PTHrP group received injections of a control adeno-associated virus (AAV) vector and an AVV vector carrying PTHrP (AAV-PTHrP) gene, respectively, followed one week later by MCD feeding for 3 weeks; the mice in the blank control were fed a normal diet for 4 weeks. Read More

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Isolation and Differentiation of Adipose-Derived Stem Cells into Odontoblast-Like Cells: A Preliminary .

Cell J 2021 Aug 17;23(3):288-293. Epub 2021 Jul 17.

School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, UK.

Objective: The aim of present study was to isolate and differentiate human adipose-derived stem cells (ASCs) into odontoblast-like cells.

Materials And Methods: In this experimental study, human adipose tissues were taken from the buccal fat pad of three individuals (mean age: 24.6 ± 2. Read More

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Anti-Inflammatory Effect of Very High Dose Local Vessel Wall Statin Administration: Poly(L,L-Lactide) Biodegradable Microspheres with Simvastatin for Drug Delivery System (DDS).

Int J Mol Sci 2021 Jul 13;22(14). Epub 2021 Jul 13.

Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, H. Sienkiewicza 112, 90-363 Lodz, Poland.

Atherosclerosis involves an ongoing inflammatory response of the vascular endothelium and vessel wall of the aorta and vein. The pleiotropic effects of statins have been well described in many in vitro and in vivo studies, but these effects are difficult to achieve in clinical practice due to the low bioavailability of statins and their first-pass metabolism in the liver. The aim of this study was to test a vessel wall local drug delivery system (DDS) using PLA microstructures loaded with simvastatin. Read More

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A Pilot Clinical Study of Hyperacute Serum Treatment in Osteoarthritic Knee Joint: Cytokine Changes and Clinical Effects.

Curr Issues Mol Biol 2021 Jul 9;43(2):637-649. Epub 2021 Jul 9.

Department of Sport Physiology, University of Physical Education, 44 Alkotás utca, 1123 Budapest, Hungary.

The serum fraction of platelet-rich fibrin (hyperacute serum) has been shown to improve cartilage cell proliferation in in vitro osteoarthritic knee joint models. We hypothesize that hyperacute serum may be a potential regenerative therapeutic for osteoarthritic knees. In this study, the cytokine milieu at the synovial fluid of osteoarthritic knee joints exposed to hyperacute serum intraarticular injections was investigated. Read More

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Age-dependent variations in haematological and serum biochemical parameters of domestic pigeons ().

Heliyon 2021 Jul 5;7(7):e07486. Epub 2021 Jul 5.

Veterinary Pathology, Ahmadu Bello University, Zaria, Nigeria.

In this study, the age-dependent variations in haematological and serum biochemical parameters of domestic pigeons were evaluated. Sixty apparently healthy domestic pigeons comprising 30 young (2-7 weeks of age) and 30 adult (>7 weeks of age) were sampled from local breeders. Blood was collected from each bird via brachial venipuncture and divided into 2 parts; one part dispensed into labeled tubes containing ethylenediaminetetraacetic acid as anticoagulant was processed for haematological analyses. Read More

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Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway.

Chin Med 2021 Jul 19;16(1):58. Epub 2021 Jul 19.

Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Road, Nanjing, 211198, China.

Background: Corydalis saxicola Bunting, affiliated with the Papaveraceae Juss., has been proven to work well in anti-inflammation, hemostasis, and analgesia. This study was designed to observe the effect and potential mechanism of Corydalis saxicola Bunting total alkaloids (CSBTA) on paclitaxel-induced peripheral neuropathy (PIPN). Read More

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Serum Extracellular Vesicle-Derived miR-124-3p as a Diagnostic and Predictive Marker for Early-Stage Acute Ischemic Stroke.

Front Mol Biosci 2021 1;8:685088. Epub 2021 Jul 1.

Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

A delay in the diagnosis of acute ischemic stroke (AIS) reduces the eligibility and outcome of patients for thrombolytic therapy. Therefore, early diagnosis and treatment of AIS are crucial. The present study evaluated the sensitivity and accuracy of serum extracellular vesicle (EV)-derived in the diagnosis and prediction of AIS. Read More

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Remdesivir Inhibits Tubulointerstitial Fibrosis in Obstructed Kidneys.

Front Pharmacol 2021 2;12:626510. Epub 2021 Jul 2.

Department of Nephrology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Kidney impairment is observed in patients with COVID-19. The effect of anti-COVID-19 agent remdesivir on kidneys is currently unknown. We aimed to determine the effect of remdesivir on renal fibrosis and its downstream mechanisms. Read More

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Uncoupling Protein 1-Independent Effects of Eicosapentaenoic Acid in Brown Adipose Tissue of Diet-Induced Obese Female Mice.

J Nutr Biochem 2021 Jul 13:108819. Epub 2021 Jul 13.

Department of Nutritional Sciences, and Obesity Research Institute, Texas Tech University, Lubbock, TX. Electronic address:

Brown adipose tissue (BAT) plays a key role in energy expenditure through its thermogenic function, making its activation a popular target to reduce obesity. We recently reported that male mice housed at thermoneutrality with uncoupling protein 1 (UCP1) deficiency had increased weight gain and glucose intolerance, but eicosapentaenoic acid (EPA) ameliorated these effects. Whether female mice respond similarly to lack of UCP1 and to EPA remains unknown. Read More

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Pre-clinical study of human umbilical cord mesenchymal stem cell transplantation for the treatment of traumatic brain injury: safety evaluation from immunogenic and oncogenic perspectives.

Neural Regen Res 2022 Feb;17(2):354-361

Basic Medical Laboratory, General Hospital of the Central Theater Command; Hubei Key Laboratory of Central Nervous System Tumor and Intervention, Wuhan, Hubei Province, China.

Stem cell therapy is a promising strategy for the treatment of traumatic brain injury (TBI). However, animal experiments are needed to evaluate safety; in particular, to examine the immunogenicity and tumorigenicity of human umbilical cord mesenchymal stem cells (huMSCs) before clinical application. In this study, huMSCs were harvested from human amniotic membrane and umbilical cord vascular tissue. Read More

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February 2022

Melatonin Protects Chronic Kidney Disease Mesenchymal Stem/Stromal Cells against Accumulation of Methylglyoxal via Modulation of Hexokinase-2 Expression.

Biomol Ther (Seoul) 2021 Jul 15. Epub 2021 Jul 15.

Department of Biochemistry, Soonchunhyang University College of Medicine, Cheonan 31151, Republic of Korea.

Treatment options for patients with chronic kidney disease (CKD) are currently limited; therefore, there has been significant interest in applying mesenchymal stem/stromal cell (MSC)-based therapy to treat CKD. However, MSCs harvested from CKD patients tend to show diminished viability and proliferation due to sustained exposure to uremic toxins in the CKD environment, which limits their utility for cell therapy. The application of melatonin has been demonstrated to improve the therapeutic efficacy of MSCs derived from and engrafted to tissues in patients suffering from CKD, although the underlying biological mechanism has not been elucidated. Read More

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Serum biochemical parameters and amino acids metabolism are altered in piglets by early-weaning and proline and putrescine supplementations.

Anim Nutr 2021 Jun 17;7(2):334-345. Epub 2021 Mar 17.

College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, Hunan, China.

The study was to investigate the effect of early-weaning stress and proline (Pro) and putrescine (Put) supplementations on serum biochemical parameters and amino acids (AA) metabolism in suckling and post-weaning pigs. Blood and small intestinal mucosa were harvested from suckling piglets at 1, 7, 14, and 21 d of age and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age, as well as from piglets received oral administration of Pro and Put from 1 to 14 d old. In suckling piglets, the serum glucose, albumin and total cholesterol levels were increased ( < 0. Read More

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Seroprevalence and associated risk factors of selected zoonotic viral hemorrhagic fevers in Tanzania.

Int J Infect Dis 2021 Jul 7;109:174-181. Epub 2021 Jul 7.

SACIDS Foundation for One Health, Sokoine University of Agriculture, Morogoro, Tanzania. Electronic address:

Objective: To determine the seroprevalence of selected zoonotic viral hemorrhagic fevers (VHFs) and their associated risk factors in Tanzania.

Methods: Blood samples were collected from consenting outpatients and community members in eight districts selected from five ecological zones of Tanzania. Serum was harvested and tested for the presence of immunoglobulin G (IgG) and M (IgM) antibodies against Crimean-Congo hemorrhagic fever (CCHF), Ebola virus disease (EVD), Marburg virus disease (MVD), Rift Valley fever (RVF), and yellow fever (YF). Read More

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Mesenchymal Stem Cells (MSCs) in Targeted Drug Delivery: Literature Review and Exploratory Data on Migrating and Differentiation Capacities of Bone MSCs into Hepatic Progenitor Cells.

Curr Top Med Chem 2021 Jul 7. Epub 2021 Jul 7.

Department of Surgical Oncology, Shaanxi Provincial People's Hospital (Third Hospital of Medical College of Xi'an Jiaotong University), Xi'an 710068, China.

Background And Objective: Mesenchymal stem cells (MSCs), particularly bone MSCs (BMSCs) offer great potentials for targeted therapeutic applications due to their migratory and differentiation capacities. Significant advances have been achieved in the differentiation of hepatocyte or hepatocyte-like cells both in vitro and in vivo. However, there is limited knowledge on the differentiation of BMSCs into bipotential hepatic progenitor cells or cholangiocytes. Read More

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Acute anejaculation, hypogonadism, and fertility preservation in the setting of neurosarcoidosis: case report and literature review.

F S Rep 2020 Dec 8;1(3):317-325. Epub 2020 Oct 8.

Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, Illinois.

Objective: To examine fertility preservation techniques in the setting of neurosarcoidosis, and to review the impact of corticosteroid and methotrexate therapy on fertility.

Design: Case report of a patient with infertility secondary to anejaculation associated with spinal neurosarcoidosis, treated with systemic corticosteroids and methotrexate.

Setting: Academic tertiary-care hospital. Read More

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December 2020

Immature Follicular Origins and Disrupted Oocyte Growth Pathways Contribute to Decreased Gamete Quality During Reproductive Juvenescence in Mice.

Front Cell Dev Biol 2021 16;9:693742. Epub 2021 Jun 16.

Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL, United States.

Egg quality dictates fertility outcomes, and although there is a well-documented decline with advanced reproductive age, how it changes during puberty is less understood. Such knowledge is critical, since advances in Assisted Reproductive Technologies are enabling pre- and peri-pubertal patients to preserve fertility in the medical setting. Therefore, we investigated egg quality parameters in a mouse model of the pubertal transition or juvenescence (postnatal day; PND 11-40). Read More

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Serum Tumor Markers Combined With Clinicopathological Characteristics for Predicting MMR and KRAS Status in 2279 Chinese Colorectal Cancer Patients: A Retrospective Analysis.

Front Oncol 2021 17;11:582244. Epub 2021 Jun 17.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Although serum tumor markers (STMs), clinicopathological characteristics and the status of KRAS and MMR play an important role in optimizing the treatment and prognosis of colorectal cancer, their interrelationships remain largely unknown. A retrospective analysis of 2279 patients who tested for KRAS and MMR status, and STM measurements prior to treatment over the past four years was conducted. Of the 784 patients tested for KRAS and 2279 patients tested for MMR status, KRAS mutations and dMMR were identified in 276 patients (35. Read More

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Serum Amyloid A Promotes Inflammation-Associated Damage and Tumorigenesis in a Mouse Model of Colitis-Associated Cancer.

Cell Mol Gastroenterol Hepatol 2021 Jul 2. Epub 2021 Jul 2.

African Cancer Institute, Department of Global Health; Department of Internal Medicine, Stellenbosch University, Stellenbosch, South Africa. Electronic address:

Background & Aims: Identifying new approaches to lessen inflammation, as well as the associated malignant consequences, remains crucial to improving the lives and prognosis of patients diagnosed with inflammatory bowel diseases. Although it previously has been suggested as a suitable biomarker for monitoring disease activity in patients diagnosed with Crohn's disease, the role of the acute-phase protein serum amyloid A (SAA) in inflammatory bowel disease remains unclear. In this study, we aimed to assess the role of SAA in colitis-associated cancer. Read More

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CHEMERIN as a modulator of angiogenesis and apoptosis processes in the corpus luteum of pigs: An in vitro study.

Biol Reprod 2021 Jun 30. Epub 2021 Jun 30.

Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

The corpus luteum (CL) undergoes rapid changes, and its functional capabilities are influenced by processes such as angiogenesis and apoptosis. According to the literature, chemerin - a protein which participates in the regulation of energy homeostasis and the immune response, may also affect angiogenesis and apoptosis. Therefore, the aim of this study was to investigate the in vitro effect of chemerin on angiogenesis and apoptosis in porcine luteal cells (Lc) during specific phases related to CL physiology. Read More

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Effect of dietary-aged maize on growth performance, nutrient utilization, and serum metabolites in broilers.

Anim Biotechnol 2021 Jun 28:1-16. Epub 2021 Jun 28.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

In China, most maize used for animal diets is stored for long periods. We examined the effects of dietary aged maize on growth performance, nutrients utilization, and serum metabolites in broilers. A total of 270 healthy 1-day-old male Cobb broilers were assigned randomly into three treatments groups and fed maize stored for different times (24 days, M0; 18 months, M18; 36 months, M36). Read More

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Intermittent administration sodium valproate has a protective effect on bone health in ovariectomized rats.

Eur J Pharmacol 2021 Sep 22;906:174268. Epub 2021 Jun 22.

Department of Trauma Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, No. 2, Zhe Shan Xi Road, Wuhu, 241001, Anhui, People's Republic of China.

The present work was aimed to evaluate the effect of different administration modes of sodium valproate (VPA) on bone strength, bone mass and bone mineral density in ovariectomized (OVX) rats and further investigation of the possible mechanism. 60 female SD rats were randomly divided into 4 groups: Sham group (Sham, n = 15), OVX group (OVX, n = 15), OVX rats received intermittent VPA treatment group (IVPA, n = 15) and OVX rats received daily VPA treatment group (EVPA, n = 15). After 12 weeks of treatment, the rats were sacrificed, and serum and femur samples were harvested. Read More

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September 2021

Stratum corneum levels of calprotectin proteins S100A8/A9 correlate with disease activity in psoriasis patients.

J Dermatol 2021 Jun 24. Epub 2021 Jun 24.

Department of Dermatology, Toho University School of Medicine, Tokyo, Japan.

Psoriasis is an intractable inflammatory skin disorder characterized by scaly erythema and plaques. The Psoriasis Area and Severity Index (PASI) is widely used to score disease severity, but evaluation is subjective, and an objective biomarker would be useful. The stratum corneum (SC), which can be non-invasively harvested, may reflect psoriasis-associated changes in epidermal keratinocytes, such as the upregulation of the calprotectin proteins S100A8 and S100A9. Read More

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Moderate-intensity aerobic training reduces cardiac damage attributable to experimental iron overload in rats.

Exp Physiol 2021 Aug 6;106(8):1772-1784. Epub 2021 Jul 6.

Department of Physiological Sciences, Federal University of Espirito Santo, Vitória, ES, Brazil.

New Findings: What is the central question of this study? The current literature indicates that oxidative stress plays a major role in iron overload. Although exercise is a well-established approach to treat/prevent cardiovascular diseases, its effects on iron overload are not known. What is the main finding and its importance? Moderate-intensity aerobic training had benefits in a rodent model of iron-overload cardiomyopathy by improving the antioxidant capacity of the heart. Read More

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Characterization and gelling properties of a bioactive extract from obtained using a chemical-free approach.

Curr Res Food Sci 2021 29;4:354-364. Epub 2021 May 29.

Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.

The bioactivity and gelling properties of a carbohydrate-rich algal extract obtained from locally harvested seaweed using a chemical-free approach were investigated for its potential interest in food applications. Physicochemical characterisation and compositional analysis of the extract, using FTIR, biochemical methods and monosaccharide analysis, confirmed the presence of alginates and fucoidans, although the main polysaccharide present in it was laminarin. Significant amounts of phenolic compounds (~9 ​mg phloroglucinol/100 ​mg sample) were also detected. Read More

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Stanozolol and Danazol Have Different Effects on Hematopoiesis in the Murine Model of Immune-Mediated Bone Marrow Failure.

Front Med (Lausanne) 2021 28;8:615195. Epub 2021 May 28.

Department of Hematology, Peking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Science, Beijing, China.

Stanozolol and danazol are widely used in the treatment of aplastic anemia; however, their mechanisms of action are unclear. Bone marrow mononuclear cells from 10 patients newly diagnosed with aplastic anemia and 10 healthy volunteers were collected and cultured together with stanozolol, danazol, or blank control separately for marrow colony assays. K562 cell lines that had been incubated with stanozolol, danazol, or blank control were tested for erythroid or megakaryocytic differentiation. Read More

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Da-Huang-Fu-Zi-Tang Ameliorates Severe Acute Pancreatitis by Elevation of M2 Kupffer Cells in Rats.

Evid Based Complement Alternat Med 2021 2;2021:5561216. Epub 2021 Jun 2.

Department of Emergency Medicine, The Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.

Introduction: Severe acute pancreatitis (SAP) is a clinical emergency often accompanied by inflammatory response syndrome (SIRS), which eventually leads to acute lung injury and failure of other organs. The activation of liver Kupffer cells (KCs) plays a major role in the process of SIRS and multiorgan damage caused by SAP. Da-Huang-Fu-Zi-Tang (DHFZT), a traditional Chinese prescription, has been widely used for SAP in the clinic. Read More

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Interactions between cattle breed type and anabolic implant strategy impact circulating serum metabolites, feedlot performance, feeding behavior, carcass characteristics, and economic return in beef steers.

Domest Anim Endocrinol 2021 May 1;77:106633. Epub 2021 May 1.

Department of Animal, Dairy and Veterinary Science, Utah State University, 4815 Old Main Hill, Logan, Utah, 84322, USA. Electronic address:

Introducing Bos indicus (BI) genetics into a beef herd has the potential to increase environmental sustainability. When introducing BI genetics, there are concerns regarding negative impacts on temperament, growth, and carcass characteristics. Implants are routinely used in the United States, with majority of cattle on feed receiving an anabolic implant to improve growth and efficiency, however research regarding the interaction between cattle breed type and anabolic implants is limited. Read More

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