4,734 results match your criteria hacat keratinocytes

CircRAB3B suppresses proliferation, motility, cell cycle progression and promotes the apoptosis of IL-22-induced keratinocytes depending on the regulation of miR-1228-3p/PTEN axis in psoriasis.

Autoimmunity 2021 Jun 7:1-10. Epub 2021 Jun 7.

Department of Dermatology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.

Introduction: Psoriasis is an immune-related chronic skin disease, and interleukin-22 (IL-22) is involved in psoriasis pathogenesis through promoting proliferation and migration abilities of keratinocytes. Here, we analysed the role of circular RNA (circRNA) RAB3B, member RAS oncogene family (circRAB3B) in regulating the phenotypes of IL-22-induced HaCaT cells.

Methods: RT-qPCR was implemented to assess RNA abundance. Read More

View Article and Full-Text PDF

MiR-221-3p as a Potential Biomarker for Patients with Psoriasis and Its Role in Inflammatory Responses in Keratinocytes.

Skin Pharmacol Physiol 2021 Jun 4:1-7. Epub 2021 Jun 4.

Department of Dermatology, Linyi City Lanshan District People's Hospital, Linyi, China.

Introduction: This study investigated serum miR-221-3p levels in psoriatic patients and the characterization of serum miR-221-3p in keratinocyte inflammatory responses was further assessed.

Methods: qRT-PCR was used to detect the expression level of miR-221-3p in the serum of 46 patients with psoriasis and 42 healthy controls. The receiver operating characteristic curve evaluated the diagnostic ability of miR-221-3p in psoriasis. Read More

View Article and Full-Text PDF

Targeted HAI-2 deletion causes excessive proteolysis with prolonged active prostasin and depletion of HAI-1 monomer in intestinal but not epidermal epithelial cells.

Hum Mol Genet 2021 Jun 4. Epub 2021 Jun 4.

Lombardi Comprehensive Cancer Center, Department of Oncology Georgetown University Washington DC 20057, USA.

Mutations of SPINT2, the gene encoding the integral membrane, Kunitz-type serine inhibitor HAI-2, primarily affect the intestine, while sparing many other HAI-2-expressing tissues, causing sodium loss in patients with syndromic congenital sodium diarrhea. The membrane-bound serine protease prostasin was previously identified as a HAI-2 target protease in intestinal tissues but not in the skin. In both tissues, the highly related inhibitor HAI-1 is, however, the default inhibitor for prostasin and the type 2 transmembrane serine protease matriptase. Read More

View Article and Full-Text PDF

Dose- and time-dependent changes in viability and IL-6, CXCL8 and CCL2 production by HaCaT-cells exposed to cobalt. Effects of high and low calcium growth conditions.

PLoS One 2021 4;16(6):e0252159. Epub 2021 Jun 4.

Department of Medical Sciences, School of Medicine and Health, Örebro University, Örebro, Sweden.

Background: Sensitization requires exposure to an allergen with subsequent production of a "danger "signal. In the skin, keratinocytes are the main producers of these signals.

Objective: To compare dose- and time-effects of cobalt on the viability of and cytokine release from HaCaT cells cultured at low or high calcium. Read More

View Article and Full-Text PDF

Mechanism of hydrogen on cervical cancer suppression revealed by high‑throughput RNA sequencing.

Oncol Rep 2021 Jul 3;46(1). Epub 2021 Jun 3.

Department of Obstetrics and Gynaecology, Changzheng Hospital, Naval Medical University, Shanghai 200003, P.R. China.

Cervical cancer is considered one of the diseases with the highest mortality among women and with limited treatment options. Hydrogen (H) inhalation has been reported to have a variety of tumor‑suppressive effects, but the exact mechanism remains unclear. In the present study, HeLa cervical cancer cells and HaCaT keratinocytes treated with H, and a HeLa xenograft mouse model subjected to H inhalation were established. Read More

View Article and Full-Text PDF

A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model.

Braz J Med Biol Res 2021 31;54(9):e10700. Epub 2021 May 31.

Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.

It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Read More

View Article and Full-Text PDF

(-)-Loliolide Isolated from Suppressed Oxidative Stress and Inflammation by Activating Nrf2/HO-1 Signaling in IFN-γ/TNF-α-Stimulated HaCaT Keratinocytes.

Antioxidants (Basel) 2021 May 27;10(6). Epub 2021 May 27.

Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea.

The present study evaluated the effects of (-)-loliolide isolated from () against oxidative stress and inflammation, and its biological mechanism in interferon (IFN)-γ/tumor necrosis factor (TNF)-α-stimulated HaCaT keratinocytes. The results showed that (-)-loliolide improved the cell viability by reducing the production of intracellular reactive oxygen species (ROS) in IFN-γ/TNF-α-stimulated HaCaT keratinocytes. In addition, (-)-loliolide effectively decreased the expression of inflammatory cytokines (interleukin (IL)-4 IL-6, IL-13, IFN-γ and TNF-α) and chemokines (CCL11 (Eotaxin), macrophage-derived chemokine (MDC), regulated on activation, normal T cell expressed and secreted (RANTES), and thymus and activation-regulated chemokine (TARC)), by downregulating the expression of epidermal-derived initial cytokines (IL-25, IL-33 and thymic stromal lymphopoietin (TSLP)). Read More

View Article and Full-Text PDF

The Phytochemical Analysis of L. Species Leaf Extracts Is Correlated with the Antioxidant, Antibacterial, and Antitumor Effects.

Molecules 2021 May 19;26(10). Epub 2021 May 19.

Faculty of Biology and Geology, Babeș-Bolyai University, 44 Republicii St., 400015 Cluj-Napoca, Romania.

The phytochemical analysis of , , , and var. leaf extracts showed species-dependent antioxidant, antibacterial, and cytotoxic effects correlated with the identified phytoconstituents. Vincamine was present in , , and var. Read More

View Article and Full-Text PDF

Inhibition of Solar UV-Induced Matrix Metalloproteinase (MMP)-1 Expression by Non-Enzymatic Softening Cherry Blossom () Extract.

Plants (Basel) 2021 May 19;10(5). Epub 2021 May 19.

Department of Food Science and Technology, Korea National University of Transportation, Chungju-si 27909, Korea.

Cherry blossom () petals are used as ingredients in many cosmetics. However, despite their use in numerous products, the exact function of cherry blossom petals in cosmetics is unclear. Therefore, we need evidence-based studies to support the labeling claims that are made in cherry blossom products in the cosmetics industry. Read More

View Article and Full-Text PDF

The Protective Effects of EMF-LTE against DNA Double-Strand Break Damage In Vitro and In Vivo.

Int J Mol Sci 2021 May 12;22(10). Epub 2021 May 12.

College of Pharmacy, Ewha Womans University, Seoul 03760, Korea.

With the rapid growth of the wireless communication industry, humans are extensively exposed to electromagnetic fields (EMF) comprised of radiofrequency (RF). The skin is considered the primary target of EMFs given its outermost location. Recent evidence suggests that extremely low frequency (ELF)-EMF can improve the efficacy of DNA repair in human cell-lines. Read More

View Article and Full-Text PDF

Ethosomes and Transethosomes for Mangiferin Transdermal Delivery.

Antioxidants (Basel) 2021 May 12;10(5). Epub 2021 May 12.

Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, I-44121 Ferrara, Italy.

Mangiferin is a natural glucosyl xanthone with antioxidant and anti-inflammatory activity, making it suitable for protection against cutaneous diseases. In this study ethosomes and transethosomes were designed as topical delivery systems for mangiferin. A preformulation study was conducted using different surfactants in association with phosphatidylcholine. Read More

View Article and Full-Text PDF

Leaf Extract Preferentially Inhibits IFN-γ-Mediated Inflammation in HaCaT Keratinocytes.

Molecules 2021 May 20;26(10). Epub 2021 May 20.

Department of Pharmacological and Molecular Sciences, University of Milan, 20133 Milan, Italy.

L. (blackcurrant) leaf extracts, due to high levels of flavonols and anthocyanins, have been shown to exhibit beneficial effects in inflammatory diseases. However, whereas their traditional use has been investigated and validated in several models of inflammation and oxidative stress, the possible impact on skin disorders is still largely unknown. Read More

View Article and Full-Text PDF

Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells.

Molecules 2021 May 4;26(9). Epub 2021 May 4.

National Institute for R&D in Chemistry and Petrochemistry-Icechim, 202, Splaiul Independentei, 060021 Bucharest, Romania.

In the present study, we evaluated for the first time the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp () discarded tissue using in vitro experimental models of skin cells exposed to ultraviolet B (UVB) irradiation and stressing agents. FBBP preparation was obtained by papain treatment of minced bones and centrifugal ultrafiltration, and the molecular weight (MW) distribution was characterized by size exclusion and reversed-phase high performance liquid chromatography (RP-HPLC). In vitro assessment of the effect of FBBP pretreatment in UVB-irradiated L929 fibroblasts and HaCaT keratinocytes revealed their cytoprotective activity. Read More

View Article and Full-Text PDF

c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation.

Front Endocrinol (Lausanne) 2021 14;12:676403. Epub 2021 May 14.

Department of Burn, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Keratinocyte differentiation dysfunction in diabetic skin is closely related to impaired skin barrier functions. We investigated the effects of c-Myc and S100A6 on Human immortal keratinocyte line (HaCaT) or keratinocyte differentiation and potential mechanisms. The expression levels of differentiation makers such as transglutaminase 1 (TGM1), loricrin (LOR), and keratin 1 (K1) were significantly reduced, while the expression of c-Myc was significantly increased in HaCaT cells cultured in high glucose and wound margin keratinocytes from diabetic rats and human patients. Read More

View Article and Full-Text PDF

miR-489-3p inhibits TLR4/NF-κB signaling to prevent inflammation in psoriasis.

Exp Ther Med 2021 Jul 11;22(1):744. Epub 2021 May 11.

College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.

Psoriasis is a chronic inflammatory skin disease whose etiology has not yet been determined. MicroRNAs (miRs) regulate the early stages of psoriasis and are targets for therapeutic intervention. The present study aimed to investigate the functional role of miR-489-3p in psoriasis. Read More

View Article and Full-Text PDF

The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways.

Redox Biol 2021 Aug 19;44:102007. Epub 2021 May 19.

Institute of Nutrition, College of Health Care, China Medical University, Taichung 40402, Taiwan. Electronic address:

Pterostilbene (Pt) is a natural polyphenol found in blueberries and several grape varieties. Pt's pharmacological importance was well documented. Nevertheless, the depigmenting effects are not demonstrated. Read More

View Article and Full-Text PDF

Glycyrrhizin improves the pathogenesis of psoriasis partially through IL-17A and the SIRT1-STAT3 axis.

BMC Immunol 2021 May 27;22(1):34. Epub 2021 May 27.

Department of Dermatology, Huashan Hospital Affiliated to Fudan University, Shanghai, 200040, China.

Background: The anti-inflammatory effect of glycyrrhizin has been widely recognized, while the specific mechanism of glycyrrhizin in psoriasis remains poorly understood.

Results: In the imiquimod-induced mouse model of psoriasis (IMD), we found that glycyrrhizin can substantially improve the adverse symptoms in mice. The hematoxylin-eosin staining results showed that glycyrrhizin can also improve the pathological state of skin cells in IMD mice. Read More

View Article and Full-Text PDF

Downregulation of Cathepsin B Reduces Proliferation and Inflammatory Response and Facilitates Differentiation in Human HaCaT Keratinocytes, Ameliorating IL-17A and SAA-Induced Psoriasis-Like Lesion.

Di Xu Jie Wang

Inflammation 2021 May 26. Epub 2021 May 26.

Department of Traditional Chinese Medicine, Ruijin Hospital Affiliated to Shanghai Jiaotong University, 197 Ruijin 2nd Road, Huangpu District, Shanghai, 200025, China.

Psoriasis is a common inflammatory dermatology disease. Strongly expressed serum amyloid A (SAA) promotes psoriasis exacerbation through inducing IL-17 secretion. What's more, SAA can stimulate the release of cathepsin B. Read More

View Article and Full-Text PDF

Investigation of cellular morphology and proliferation on patterned electrospun PLA-gelatin mats.

J Biol Phys 2021 Jun 25;47(2):205-214. Epub 2021 May 25.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.

The morphology and proliferation of eukaryotic cells depend on their microenvironment. When electrospun mats are used as tissue engineering scaffolds, the local alignment of the fibers has a pronounced influence on cells. Here we analyzed the morphology of the patterned mats produced by electrospinning of PLA-gelatin blend onto a conductive grid. Read More

View Article and Full-Text PDF

Dynamic alteration in miRNA and mRNA expression profiles at different stages of chronic arsenic exposure-induced carcinogenesis in a human cell culture model of skin cancer.

Arch Toxicol 2021 May 25. Epub 2021 May 25.

Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY, USA.

Chronic arsenic exposure causes skin cancer, although the underlying molecular mechanisms are not well defined. Altered microRNA and mRNA expression likely play a pivotal role in carcinogenesis. Changes in genome-wide differential expression of miRNA and mRNA at 3 strategic time points upon chronic sodium arsenite (As) exposure were investigated in a well-validated HaCaT cell line model of arsenic-induced cutaneous squamous cell carcinoma (cSCC). Read More

View Article and Full-Text PDF

Protective effects of dissolved molecular hydrogen against hydrogen peroxide-, hydroperoxide-, and glyoxal-induced injuries to human skin keratinocytes.

Mol Cell Biochem 2021 May 24. Epub 2021 May 24.

Laboratory of Bioscience & Biotechnology for Cell Function Control, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 5562 Nanatsuka, Shobara, Hiroshima, 727-0023, Japan.

Molecular hydrogen (H) is recognized as a gaseous antioxidant, and it is expected to ameliorate various disorders related to oxidative stress and inflammation. However, there are still many unclear points regarding its effectiveness in the skin. Therefore, the purpose of this study was to examine the protective effect of H against ultraviolet (UV) irradiation-related stress injury in human epidermal HaCaT cells. Read More

View Article and Full-Text PDF

Hyperforin Ameliorates Imiquimod-Induced Psoriasis-Like Murine Skin Inflammation by Modulating IL-17A-Producing γδ T Cells.

Front Immunol 2021 5;12:635076. Epub 2021 May 5.

Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Hyperforin is a major active constituent of  L. extract, which is widely used for the treatment of depressive disorders. Recent studies have reported that hyperforin reduced inflammation in stroke and suppressed proliferation and differentiation in keratinocytes. Read More

View Article and Full-Text PDF

Korean Red Ginseng attenuates ultraviolet-mediated inflammasome activation in keratinocytes.

J Ginseng Res 2021 May 13;45(3):456-463. Epub 2021 Feb 13.

College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Republic of Korea.

Background: Keratinocytes form a physical barrier and act as an innate immune cell in skin. Keratinocytes secrete pro-inflammatory cytokines, such as interleukin (IL)-1β, resulting from inflammasome activation when exposed to ultraviolet (UV) irradiation. Korean Red Ginseng extracts (RGE) have been well-studied as modulators of inflammasome activation in immune cells, such as macrophages. Read More

View Article and Full-Text PDF

Uptake, intracellular dissolution, and cytotoxicity of silver nanowires in cell models.

Chemosphere 2021 May 12;281:130762. Epub 2021 May 12.

School of Energy and Environment and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong, China; Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China. Electronic address:

The uptake, intracellular dissolution, and cytotoxicity of silver nanowires (AgNWs) in two cell models (human keratinocytes - HaCaT cells and murine macrophages) were systemically investigated for the first time. Cellular uptake of AgNWs occurred mainly via pathways of clathrin-dependent endocytosis, caveolae-dependent endocytosis, and phagocytosis. AgNWs could be internalized by two types of cells with numerous lysosomal vesicles detected in close vicinity to AgNWs. Read More

View Article and Full-Text PDF

Impact of mono-culture vs. Co-culture of keratinocytes and monocytes on cytokine responses induced by important skin sensitizers.

J Immunotoxicol 2021 Dec;18(1):74-84

Institute of Environmental Medicine, Karolinska Institute, Stockholm, Sweden.

Sensitization to a contact allergen brings with it a lifelong risk to develop allergic contact dermatitis. Inflammation is an important part of the skin sensitizing mechanism, and understanding how different haptens stimulate the immune system, as well as the role played by different cell types present in skin, may be helpful for developing optimized models for risk assessment of new chemicals or mixtures. The aim of this study was to compare the cytokine profile following exposure of cells representing keratinocytes (HaCaT), monocytes (THP-1) and a co-culture of these cells to three clinically important skin sensitizers: cobalt (II) chloride (CoCl), methylisothiazolinone (MI) and p-phenylenediamine (PPD). Read More

View Article and Full-Text PDF
December 2021

Sortilin regulates keratinocyte proliferation and apoptosis through the PI3K-AKT signaling pathway.

Life Sci 2021 Aug 15;278:119630. Epub 2021 May 15.

Department of Dermatology, The Second Affiliated Hospital of Soochow University, Su Zhou 215004, China. Electronic address:

Sortilin is found to regulate proliferation and death of different cells, while its role in regulating keratinocyte proliferation and apoptosis is still unknown. In this study, we found that sortilin levels significantly increased in psoriasis patients, and sortilin suppression eliminated the proliferation of HaCaT cells induced by M5 cocktail solution and enhanced the levels of cleaved caspase 3 protein and the Bax/Bcl-2 ratio; however, levels of p-PI3K and p-AKT were decreased. In addition, sortilin silencing remitted the characteristic changes associated with psoriasis-like skin lesions. Read More

View Article and Full-Text PDF

Long-wavelength UVA enhances UVB-induced cell death in cultured keratinocytes: DSB formation and suppressed survival pathway.

Photochem Photobiol Sci 2021 May 12;20(5):639-652. Epub 2021 May 12.

National Institute of Occupational Safety and Health, Kawasaki, Japan.

Solar UV radiation consists of both UVA and UVB. The wavelength-specific molecular responses to UV radiation have been studied, but the interaction between UVA and UVB has not been well understood. In this study, we found that long-wavelength UVA, UVA1, augmented UVB-induced cell death, and examined the underlying mechanisms. Read More

View Article and Full-Text PDF

Cytotoxic effects of wildfire ashes: In-vitro responses of skin cells.

Environ Pollut 2021 Apr 30;285:117279. Epub 2021 Apr 30.

CESAM (Centre for Environmental and Marine Studies), University of Aveiro, Aveiro, Portugal; Department of Environment and Planning, University of Aveiro, Aveiro, Portugal.

Wildfires are a complex environmental problem worldwide. The ashes produced during the fire bear metals and PAHs with high toxicity and environmental persistence. These are mobilized into downhill waterbodies, where they can impair water quality and human health. Read More

View Article and Full-Text PDF

HIF1 may promote glycolysis in psoriasis vulgaris via upregulation of CD147 and GLUT1.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):333-344

Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha 410008.

Objectives: To analyze the expressions and distributions of hypoxia-inducible factor-1α (HIF-1α), CD147, and glucose transporter 1 (GLUT1) in epidermis from psoriasis vulgaris and normal people, and to explore the associations among these proteins and their roles in hypoxic HaCaT cell line.

Methods: The expression levels of HIF-1α, CD147, and GLUT1 were determined by immunohistochemistry staining in skin biopsies from 48 psoriasis vularis patients and 33 healthy subjects. Cobalt chloride (CoCl) was added into the culture media of HaCaT cells to mimic hypoxia while RNA interference and transfection technologies were used to explore the association among these proteins by quantitative real-time polymerase chain reaction and Western blotting. Read More

View Article and Full-Text PDF

Epsilon toxin from Clostridium perfringens induces toxic effects on skin tissues and HaCaT and human epidermal keratinocytes.

Toxicon 2021 Jul 7;198:102-110. Epub 2021 May 7.

State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, PR China. Electronic address:

Epsilon toxin (ETX) is a key pathogenic factor of C. perfringens type B and D, causing fatal enterotoxemia in sheep and goats. Excessive production of ETX increases intestinal permeability; its entrance into the bloodstream leads to severe edema in organs such as the brain and kidneys. Read More

View Article and Full-Text PDF