Ther Drug Monit 2021 Apr 13. Epub 2021 Apr 13.
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University of Science, Hokkaido, Japan Department of Pharmacy, University of Tsukuba Hospital, Ibaraki, Japan Pharmaceutical Division, Sapporo Minami-sanjo Hospital, Hokkaido, Japan Biotage Japan Ltd., Tokyo, Japan Department of Pharmacotherapy, Faculty of Pharmaceutical Sciences, Hokkaido University of Science, Hokkaido, Japan Department of Pulmonary Diseases, Sapporo Minami-sanjo Hospital, Hokkaido, Japan Nihon Pharmaceutical University, Saitama, Japan.
Background: Routine therapeutic drug monitoring is a promising approach for the rational use of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) and anaplastic lymphoma kinase (ALK) inhibitors. The purpose of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of five EGFR-TKIs (afatinib, dacomitinib, erlotinib, gefitinib, and osimertinib) and three ALK inhibitors (alectinib, ceritinib, and crizotinib).
Methods: A 100-microliter aliquot of serum was diluted with 100 μL of 1% aqueous ammonia containing internal standards and then purified using the supported liquid extraction method. Read More