2,864 results match your criteria group c-type

Implant failure following pedicle based dynamic stabilization of the lumbar spine.

Acta Orthop Belg 2021 Mar;87(1):191-196

Pedicle-based dynamic stabilization (PBDS) devices such as Dynesys are promoted as an alternative and less invasive option for rigid stabilization of one and even more levels of the lumbar spine. Promising features of the Dynesys system, as well as shortcomings, became obvious in several clinical studies. Since 2012, we started using a new PBDS device as an alternative for the Dynesys, to avoid the screw loosening and the kyphosing effect. Read More

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MelLec Exacerbates the Pathogenesis of -Induced Allergic Inflammation in Mice.

Front Immunol 2021 28;12:675702. Epub 2021 May 28.

Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.

Environmental factors, particularly fungi, influence the pathogenesis of allergic airway inflammation, but the mechanisms underlying these effects are still unclear. Melanin is one fungal component which is thought to modulate pulmonary inflammation. We recently identified a novel C-type lectin receptor, MelLec (Clec1a), which recognizes fungal 1,8-dihydroxynaphthalene (DHN)-melanin and is able to regulate inflammatory responses. Read More

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CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.

FASEB J 2021 Jul;35(7):e21711

Department of Pharmacology, School of Basic Medical Sciences, Shandong University, Jinan, China.

Podocyte injury is a major determinant of focal segmental glomerular sclerosis (FSGS) and the identification of potential therapeutic targets for preventing podocyte injury has clinical importance for the treatment of FSGS. CLEC14A is a single-pass transmembrane glycoprotein belonging to the vascular expressed C-type lectin family. CLEC14A is found to be expressed in vascular endothelial cells during embryogenesis and is also implicated in tumor angiogenesis. Read More

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Incommensurately modulated crystal structure of α' (O'3)-type sodium cobalt oxide NaCoO (x ∼ 0.78).

Acta Crystallogr B Struct Sci Cryst Eng Mater 2021 Jun 14;77(Pt 3):371-377. Epub 2021 May 14.

Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.

A single-phase sample of α' (O'3)-type layered sodium cobalt oxide NaCoO (x ∼ 0.78) was prepared and its incommensurately modulated crystal structure was analyzed using the (3+1)-dimensional superspace approach to the powder neutron diffraction data. The crystal structure of the cobaltate is accurately described based on the superspace group C2/m(α0γ)00, wherein the positions of Na atoms are most significantly modulated in the monoclinic a direction to form an ordered arrangement. Read More

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Can Plant Lectins Help to Elucidate Insect Lectin-Mediated Immune Response?

Insects 2021 May 27;12(6). Epub 2021 May 27.

Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.

Lectins are carbohydrate-binding proteins that recognize and selectively bind to specific sugar structures. This group of proteins is widespread in plants, animals, and microorganisms, and exerts a broad range of functions. Many plant lectins were identified as exogenous stimuli of vertebrate immunity. Read More

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Pinosylvin Shifts Macrophage Polarization to Support Resolution of Inflammation.

Molecules 2021 May 8;26(9). Epub 2021 May 8.

The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University, Tampere University Hospital, 33014 Tampere, Finland.

Pinosylvin is a natural stilbenoid found particularly in Scots pine. Stilbenoids are a group of phenolic compounds identified as protective agents against pathogens for many plants. Stilbenoids also possess health-promoting properties in humans; for instance, they are anti-inflammatory through their suppressing action on proinflammatory M1-type macrophage activation. Read More

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SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.

Immunity 2021 06 9;54(6):1304-1319.e9. Epub 2021 May 9.

Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06519, USA.

Despite mounting evidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) engagement with immune cells, most express little, if any, of the canonical receptor of SARS-CoV-2, angiotensin-converting enzyme 2 (ACE2). Here, using a myeloid cell receptor-focused ectopic expression screen, we identified several C-type lectins (DC-SIGN, L-SIGN, LSECtin, ASGR1, and CLEC10A) and Tweety family member 2 (TTYH2) as glycan-dependent binding partners of the SARS-CoV-2 spike. Except for TTYH2, these molecules primarily interacted with spike via regions outside of the receptor-binding domain. Read More

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Molecular mechanism of N-terminal acetylation by the ternary NatC complex.

Structure 2021 May 15. Epub 2021 May 15.

Department of Chemistry, 231 South 34(th) Street, University of Pennsylvania, Philadelphia, PA 19104, USA; Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104, USA. Electronic address:

Protein N-terminal acetylation is predominantly a ribosome-associated modification, with NatA-E serving as the major enzymes. NatC is the most unusual of these enzymes, containing one Naa30 catalytic subunit and two auxiliary subunits, Naa35 and Naa38; and substrate selectivity profile that overlaps with NatE. Here, we report the cryoelectron microscopy structure of S. Read More

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Homogeneous Cytochrome 579 Is an Octamer That Reacts Too Slowly With Soluble Iron to Be the Initial Iron Oxidase in the Respiratory Chain of .

Front Microbiol 2021 3;12:673066. Epub 2021 May 3.

Department of Bioinformatics and Genomics, University of North Carolina, Charlotte, NC, United States.

The exact role that cytochrome 579 plays in the aerobic iron respiratory chain of is unclear. This paper presents genomic, structural, and kinetic data on the cytochrome 579 purified from cell-free extracts of cultured on soluble iron. Electrospray mass spectrometry of electrophoretically homogeneous cytochrome 579 yielded two principal peaks at 16,015 and 16,141 Daltons. Read More

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The Role of Lectin Receptors and Their Ligands in Controlling Allergic Inflammation.

Front Immunol 2021 30;12:635411. Epub 2021 Apr 30.

Department of Molecular Immunology, Ruhr-University Bochum, Bochum, Germany.

More than fifty c-type lectin receptors (CLR) are known and have been identified so far. Moreover, we know the group of galectins and sialic acid-binding immunoglobulin-type lectins that also belong to the carbohydrate-binding receptors of the immune system. Thus, the lectin receptors form the largest receptor family among the pathogen recognition receptors. Read More

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Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms.

Sci Rep 2021 May 12;11(1):10141. Epub 2021 May 12.

VPr1 Research Group "Molecular Allergology", Paul-Ehrlich-Institut, Langen, Germany.

Evidence has suggested that major peanut allergen Ara h 1 activates dendritic cells (DCs) via interaction with DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin), a C-type lectin receptor, and contributes to development of peanut allergy. Since macrophages, as well as DCs, play a crucial role in innate immunity, we investigated whether natural Ara h 1 (nAra h 1) activates two different subsets of macrophages, human monocyte derived macrophage type 1 (hMDM1: pro-inflammatory model) and type 2 (hMDM2: anti-inflammatory model). hMDM1 and hMDM2 predominantly produced pro-inflammatory cytokines (IL-6 and TNF-α) and an anti-inflammatory cytokine (IL-10) in response to nAra h 1, respectively. Read More

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Maternal plasma pro-atrial and C-type natriuretic peptide levels and their associations with cardiovascular and renal function in the second half of normal pregnancy: a longitudinal study.

BMC Pregnancy Childbirth 2021 May 5;21(1):358. Epub 2021 May 5.

Department of Clinical Medicine, Women's Health and Perinatology Research Group, UiT-The Arctic University of Norway, Tromsø, Norway.

Background: Physiological adaptation in pregnancy is characterized by remodeling of endocrine, cardiovascular and renal functions leading to fluid retention, volume expansion, altered cardiac loading conditions and hyperdynamic circulation. Natriuretic peptides have been used as biomarkers of cardiovascular function, but their associations with gestational age-related changes in maternal cardiac, endothelial and renal function have not been elucidated. The aim of this study was to establish longitudinal reference values for maternal plasma atrial natriuretic peptide (proANP) and C-type natriuretic peptide (CNP) and investigate their temporal association with cardiovascular and renal function in the second half of pregnancy. Read More

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Divergent Cytochrome Maturation System in Kinetoplastid Protists.

mBio 2021 05 4;12(3). Epub 2021 May 4.

School of Applied Sciences, University of Huddersfield, Huddersfield, United Kingdom

In eukaryotes, heme attachment through two thioether bonds to mitochondrial cytochromes and is catalyzed by either multisubunit cytochrome maturation system I or holocytochrome synthetase (HCCS). The former was inherited from the alphaproteobacterial progenitor of mitochondria; the latter is a eukaryotic innovation for which prokaryotic ancestry is not evident. HCCS provides one of a few exemplars of protein innovation in eukaryotes, but structure-function insight of HCCS is limited. Read More

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Fe-Enrichment effect on the composition and performance of Fe-based O-reduction electrocatalysts.

Phys Chem Chem Phys 2021 Apr;23(15):9147-9157

Paul Scherrer Institut, 5232 Villigen PSI, Switzerland.

Pt-group metal (PGM)-free catalysts of the Me-N-C type based on abundant and inexpensive elements have gained importance in the field of oxygen reduction reaction (ORR) electrocatalysis due to their promising ORR-activities. Their insufficient stability, however, has fueled the interest in obtaining an in-depth understanding of their composition, which requires highly sensitive techniques compatible with their low metal contents (typically <5 wt%). In the particular context of iron-based materials, 57Fe-Mössbauer spectroscopy is often used to provide such compositional information, but requires (partially) 57Fe-enriched precursors. Read More

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Synthesis of the Fungal Metabolite YWA1 and Related Constructs as Tools to Study MelLec-Mediated Immune Response to Infections†.

J Org Chem 2021 May 22;86(9):6044-6055. Epub 2021 Apr 22.

Kosterlitz Centre for Therapeutics, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, U.K.

We describe the chemical synthesis of the fungal naphthopyrones YWA1 and fonsecin B, as well as their functionalization with an amine-spacer arm and the conjugation of the resulting molecules to three different functional tags (i.e., biotin, Oregon green, 1-[3-(succinimidyloxycarbonyl)benzyl]-4-[5-(4-methoxyphenyl)-2-oxazolyl]pyridinium bromide (PyMPO)). Read More

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Identification and expression analysis of group II C-type lectin domain containing receptors in grass carp Ctenopharyngodon idella.

Gene 2021 Jul 18;789:145668. Epub 2021 Apr 18.

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address:

Group II C-type lectin domain (CTLD) containing receptors belong to a large family of pattern recognition receptors which mainly act on the innate immunity. They are structurally related and consist of a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and a single extracellular CTLD. Although they have been described in teleost fish, their involvement in immune responses is not well understood. Read More

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Trehalose diamide glycolipids augment antigen-specific antibody responses in a Mincle-dependent manner.

Bioorg Chem 2021 May 4;110:104747. Epub 2021 Mar 4.

School of Chemical and Physical Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand; Centre for Biodiscovery, Victoria University of Wellington, PO Box 600, Wellington, New Zealand. Electronic address:

Many studies have investigated how trehalose glycolipid structures can be modified to improve their Macrophage inducible C-type lectin (Mincle)-mediated adjuvanticity. However, in all instances, the ester-linkage of α,ά-trehalose to the lipid of choice remained. We investigated how changing this ester-linkage to an amide influences Mincle signalling and agonist activity and demonstrated that Mincle tolerates this functional group change. Read More

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Expanding the Known Repertoire of C-Type Lectin Receptors Binding to Toxoplasma gondii Oocysts Using a Modified High-Resolution Immunofluorescence Assay.

mSphere 2021 03 31;6(2). Epub 2021 Mar 31.

Robert Koch-Institute, FG16: Mycotic and Parasitic Agents and Mycobacteria, Berlin, Germany

The environmental stage of the apicomplexan oocyst is vital to its life cycle but largely understudied. Because oocysts are excreted only by infected felids, their availability for research is limited. We report the adaptation of an agarose-based method to immobilize minute amounts of oocysts to perform immunofluorescence assays. Read More

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C-Type Lectin-Like Molecule-1 as a Biomarker for Diagnosis and Prognosis in Acute Myeloid Leukemia: A Preliminary Study.

Biomed Res Int 2021 11;2021:6643948. Epub 2021 Mar 11.

Department of Hematology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objective: AML is a heterogeneous disease both in genomic and proteomic backgrounds, and variable outcomes may appear in the same cytogenetic risk group. Therefore, it is still necessary to identify new antigens that contribute to diagnostic information and to refine the current risk stratification.

Methods: The expression of C-type lectin-like molecule-1 (CLL-1) in AML blasts was examined in 52 patients with newly diagnosed or relapsed/refractory AML and was compared with two other classic markers CD33 and CD34 in AML, in order to assess the value of CLL-1 as an independent biomarker or in combination with other markers for diagnosis in AML. Read More

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High Expression of CLEC11A Predicts Favorable Prognosis in Acute Myeloid Leukemia.

Front Oncol 2021 2;11:608932. Epub 2021 Mar 2.

Department of Hematology, Chinese People's Liberation Army General Hospital, Beijing, China.

Background: Acute myeloid leukemia (AML) is a heterogeneous disease of the hematopoietic system, for which identification of novel molecular markers is potentially important for clinical prognosis and is an urgent need for treatment optimization.

Methods: We selected C-type lectin domain family 11, member A (CLEC11A) for study several public databases, comparing expression among a variety of tumors and normal samples as well as different organs and tissues. To investigated the relationship between CLEC11A expression and clinical characteristics, we derived an AML cohort from The Cancer Genome Atlas (TCGA); we also investigated the Bloodspot and HemaExplorer databases. Read More

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Halogen-Bond Mediated 3D Confined Assembly of AB Diblock Copolymer and C Homopolymer Blends.

Small 2021 May 18;17(18):e2007570. Epub 2021 Mar 18.

Key Laboratory of Materials Chemistry for Energy Conversion and Storage, Ministry of Education (HUST), School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, 430074, China.

Halogen-bond driven assembly, a world parallel to hydrogen-bond, has emerged as an attractive tool for constructing (macro)molecular arrangement. However, knowledge about halogen-bond mediated confined-assembly in emulsion droplets is limited so far. An I N bond mediated confined-assembly pathway to enable order-order phase transitions is reported here. Read More

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Dependence on Mincle and Dectin-2 Varies With Multiple Species During Systemic Infection.

Front Microbiol 2021 25;12:633229. Epub 2021 Feb 25.

Division of Infection and Immunity, Systems Immunity Research Institute, Cardiff University School of Medicine, Cardiff, United Kingdom.

More than 95% of invasive infections are caused by four spp. (). C-type lectin-like receptors (CLRs), such as Dectin-1, Dectin-2, and Mincle mediate immune responses to . Read More

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February 2021

Genetic diversity of sp. paratuberculosis by mycobacterial interspersed repetitive Unit-Variable number tandem repeat and multi-locus short-sequence repeat one-sentence summary: Genetic diversity of sp. paratuberculosis isolates.

Int J Mycobacteriol 2021 Jan-Mar;10(1):51-59

Institute of Agrobiotechnology and Molecular Biology (IABIMO)-CONICET, INTA De Los Reseros y Las Cabañas S/N, Hurlingham, Argentina.

Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Read More

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Sensitivity of the Natriuretic Peptide/cGMP System to Hyperammonaemia in Rat C6 Glioma Cells and GPNT Brain Endothelial Cells.

Cells 2021 Feb 15;10(2). Epub 2021 Feb 15.

Endocrine Signalling Group, Department of Comparative Biomedical Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK.

C-type natriuretic peptide (CNP) is the major natriuretic peptide of the central nervous system and acts via its selective guanylyl cyclase-B (GC-B) receptor to regulate cGMP production in neurons, astrocytes and endothelial cells. CNP is implicated in the regulation of neurogenesis, axonal bifurcation, as well as learning and memory. Several neurological disorders result in toxic concentrations of ammonia (hyperammonaemia), which can adversely affect astrocyte function. Read More

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February 2021

Developing a Vaccine to Block West Nile Virus Transmission: In Silico Studies, Molecular Characterization, Expression, and Blocking Activity of mosGCTL-1.

Pathogens 2021 Feb 17;10(2). Epub 2021 Feb 17.

Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Pasteur Ave., Tehran 1316943551, Iran.

Background: Mosquito galactose-specific C-type lectins (mosGCTLs), such as mosGCTL-1, act as ligands to facilitate the invasion of flaviviruses like West Nile virus (WNV). WNV interacts with the of (Culicidae) and facilitates the invasion of this virus. Nevertheless, there is no data about the role of mosGCTL-1 as a transmission-blocking vaccine candidate in , the most abundant Culicinae mosquito in temperate regions. Read More

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February 2021

Ozone nanobubble modulates the innate defense system of Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae.

Fish Shellfish Immunol 2021 May 2;112:64-73. Epub 2021 Mar 2.

Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, 10300, Thailand. Electronic address:

Ozone nanobubble (NB-O) is a promising technology for improving dissolved oxygen and reducing bacterial concentration in aquaculture systems. Here, we investigated the effects of NB-O on the innate immunity of fish by monitoring the expression levels of nonspecific immune-related genes (IL-1β, IL-2β, TNF-α), heat-shock protein genes (HSP70, HSP90-α), and a bacteriolytic enzyme, C-type lysozyme, gene (LYZ) post-treatment with this technology. Following exposure to NB-O, the different tissues of Nile tilapia (Oreochromis niloticus) were collected over time for quantitative real-time PCR (qPCR) analysis. Read More

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Evaluation of the relationship of lymphangiogenesis markers with disease pathogenesis in patients with Behçet's uveitis.

Acta Clin Belg 2021 Feb 25:1-9. Epub 2021 Feb 25.

Department of Ophthalmology, Gülhane Training and Research Hospital, University of Health Sciences, Ankara, Turkey.

: The present study aims to evaluate the relationship between Behçet's uveitis and lymphangiogenesis by determining levels of Vascular endothelial growth factor-C (VEGF-C, its receptors sVEGFR-2, sVEGFR-3 and lymphangiogenesis markers podoplanin (PDPN) and lymphatic vessel endothelial hyaluronan receptor 1(LYVE-1), and C-type lectin domain family 1 member B (CLEC2).: 55 patients with BD uveitis and 31 healthy control subjects were enrolled in the study.: sVEGFR-2, sVEGFR-3, VEGF-C/sVEGFR-2 ratio, PDPN and LYVE-1 levels were higher in the patient group. Read More

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February 2021

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo.

J Vis Exp 2021 02 5(168). Epub 2021 Feb 5.

Immune Regulation Group, Helmholtz Centre for Infection Research; Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg.

Targeted antigen delivery to cross-presenting dendritic cells (DC) in vivo efficiently induces T effector cell responses and displays a valuable approach in vaccine design. Antigen is delivered to DC via antibodies specific for endocytosis receptors such as DEC-205 that induce uptake, processing, and MHC class I- and II-presentation. Efficient and reliable conjugation of the desired antigen to a suitable antibody is a critical step in DC targeting and among other factors depends on the format of the antigen. Read More

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February 2021

Potential-Induced Spin Changes in Fe/N/C Electrocatalysts Assessed by In Situ X-ray Emission Spectroscopy.

Angew Chem Int Ed Engl 2021 May 12;60(21):11707-11712. Epub 2021 Apr 12.

Paul Scherrer Institut, Forschungsstrasse 111, 5232, Villigen PSI, Switzerland.

The commercial success of the electrochemical energy conversion technologies required for the decarbonization of the energy sector requires the replacement of the noble metal-based electrocatalysts currently used in (co-)electrolyzers and fuel cells with inexpensive, platinum-group metal-free analogs. Among these, Fe/N/C-type catalysts display promising performances for the reduction of O or CO , but their insufficient activity and stability jeopardize their implementation in such devices. To circumvent these issues, a better understanding of the local geometric and electronic structure of their catalytic active sites under reaction conditions is needed. Read More

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