797 results match your criteria grapevine virus

Evidence of differential spreading events of grapevine pinot Gris virus in Italy using datamining as a tool.

Eur J Plant Pathol 2021 Aug 26:1-8. Epub 2021 Aug 26.

Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France.

Since its identification in 2003, grapevine Pinot gris virus (GPGV, ) has now been detected in most grape-growing countries. So far, little is known about the epidemiology of this newly emerging virus. In this work, we used datamining as a tool to monitor the sanitary status of three vineyards in Italy. Read More

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Gimme shelter: three-dimensional architecture of the endoplasmic reticulum, the replication site of grapevine Pinot gris virus.

Funct Plant Biol 2021 Sep;48(10):1074-1085

Department of Agriculture, Food, Environmental and Animal Sciences, University of Udine,via delle Scienze, 206, 33100 - Udine, Italy; and Corresponding author. Email:

Grapevine leaf mottling and deformation is a novel grapevine disease that has been associated with grapevine Pinot gris virus (GPGV). The virus was observed exclusively inside membrane-bound structures in the bundle sheath cells of the infected grapevines. As reported widely in the literature, many positive-sense single-stranded RNA viruses modify host-cell membranes to form a variety of deformed organelles, which shelter viral genome replication from host antiviral compounds. Read More

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September 2021

Impact of Rootstock and Season on Red Blotch Disease Expression in Cabernet Sauvignon ().

Plants (Basel) 2021 Jul 31;10(8). Epub 2021 Jul 31.

Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.

Grapevine red blotch virus (GRBV), the causative agent of grapevine red blotch disease, is widespread across the United States and causes a delay in ripening events in grapes. This study evaluates the effects of GRBV on Cabernet Sauvignon grape berry composition, grafted on two different rootstocks (110R and 420A) in two seasons (2016 and 2017). Total soluble solids, acidity, and anthocyanin concentrations were monitored through ripening and at harvest. Read More

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First report of Grapevine enamovirus 1 in Vitis vinifera cultivar 'Meunier' in France.

Plant Dis 2021 Aug 22. Epub 2021 Aug 22.

Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France, Colmar, France;

Grapevine enamovirus 1 (GEV-1) is a member of the genus Enamovirus in the family Solemoviridae. GEV-1 was first described in 2017 in a few grapevine cultivars in Brazil (Silva et al. 2017) and subsequently in China (Ren et al. Read More

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High-Throughput Sequencing Indicates a Novel Marafivirus in Grapevine Showing Vein-Clearing Symptoms.

Plants (Basel) 2021 Jul 20;10(7). Epub 2021 Jul 20.

National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.

A putative new marafivirus was identified in a 'Jumeigui' grapevine exhibitting obvious vein-clearing symptoms by high-throughput sequencing, which tentatively named grapevine-associated marafivirus (GaMV). The nearly complete genomic sequence of GaMV was amplified by reverse transcription PCR, and the terminal sequences were determined using the rapid amplification of cDNA ends method. The nearly complete genome of GaMV is 6346 bp long, excluding the poly(A) tail, and shows 51. Read More

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Metagenomic analysis of nepoviruses: diversity, evolution and identification of a genome region in members of subgroup A that appears to be important for host range.

Arch Virol 2021 Oct 9;166(10):2789-2801. Epub 2021 Aug 9.

Université de Strasbourg, INRAE, SVQV, UMR-A 1131, F-68000, Colmar, France.

Data mining and metagenomic analysis of 277 open reading frame sequences of bipartite RNA viruses of the genus Nepovirus, family Secoviridae, were performed, documenting how challenging it can be to unequivocally assign a virus to a particular species, especially those in subgroups A and C, based on some of the currently adopted taxonomic demarcation criteria. This work suggests a possible need for their amendment to accommodate pangenome information. In addition, we revealed a host-dependent structure of arabis mosaic virus (ArMV) populations at a cladistic level and confirmed a phylogeographic structure of grapevine fanleaf virus (GFLV) populations. Read More

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October 2021

High-Throughput Sequencing of Small RNAs for the Sanitary Certification of Viruses in Grapevine.

Front Plant Sci 2021 21;12:682879. Epub 2021 Jul 21.

Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario, Murcia, Spain.

Biological indexing is the method generally recognized for the certification of propagative grapevines in many countries, and it is mandatory in the European Union. It consists of the evaluation of the plant material after grafting on indicators that are inspected for symptom development. This is a lengthy process that requires well-trained workers, testing field, etc. Read More

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Emerging and population analysis of isolates from Iran.

3 Biotech 2021 Aug 7;11(8):368. Epub 2021 Jul 7.

Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

(GPGV) is a novel member of genus in family. During 2018-2019, 114 leaf and green shoot samples were collected from the main vineyards in Iran Zanjan, Hamedan and East Azerbaijan provinces. After total RNA extraction and cDNA synthesis, the samples were tested by PCR assay using two pairs of specific primers corresponding to the coat protein (CP) and movement protein (MP) regions of GPGV, in which 6 out of 114 samples were found to be infected by GPGV. Read More

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First report of grapevine rupestris vein feathering virus in L. from Japan.

Plant Dis 2021 Jul 7. Epub 2021 Jul 7.

University of Yamanashi, 38146, The Institute of Enology and Viticulture, Kofu, Yamanashi, Japan;

The production of wine grapes is gaining widespread popularity and being carried out on approximately 2,200 hectares of land in Japan. Scions grafted onto rootstocks generally have been imported from the EU, USA, New Zealand, and Australia into Japan. Unfortunately, viruses have spread in Japanese vineyards by slipping through the net of plant quarantine. Read More

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First Report of Grapevine Kizil Sapak Virus in Grapevine in China.

Plant Dis 2021 Jul 6. Epub 2021 Jul 6.

National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, China;

Grapevine Kizil Sapak virus (GKSV) is a novel member of the family Betaflexiviridae classified into the proposed genus Fivivirus within the subfamily Trivirinae. It was first discovered in USA from a grapevine originating from Turkmenistan (Al Rwahnih et al. 2019) and later in France from a grapevine accession from Iran (Marais et al. Read More

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Quality Assessment and Validation of High-Throughput Sequencing for Grapevine Virus Diagnostics.

Viruses 2021 06 11;13(6). Epub 2021 Jun 11.

Department of Plant Pathology, University of California-Davis, Davis, CA 95616, USA.

Development of High-Throughput Sequencing (HTS), also known as next generation sequencing, revolutionized diagnostic research of plant viruses. HTS outperforms bioassays and molecular diagnostic assays that are used to screen domestic and quarantine grapevine materials in data throughput, cost, scalability, and detection of novel and highly variant virus species. However, before HTS-based assays can be routinely used for plant virus diagnostics, performance specifications need to be developed and assessed. Read More

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Variable Populations of Grapevine Virus T Are Present in Vineyards of Hungary.

Viruses 2021 06 10;13(6). Epub 2021 Jun 10.

Institute of Plant Protection, Hungarian University of Agriculture and Life Sciences, Ménesi Road 44, H-1118 Budapest, Hungary.

Grapevine virus T (GVT) is a recently described foveavirus, which was identified from a transcriptome of a Teroldego grapevine cultivar in 2017. Recently, we surveyed vineyards and rootstock plantations in Hungary using small RNA (sRNA) high-throughput sequencing (HTS), at a time when GVT had not yet been described. A re-analysis of our sRNA HTS datasets and a survey of grapevines by RT-PCR revealed the presence of GVT in most of the vineyards tested, while at rootstock fields its presence was very rare. Read More

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A novel ampelovirus associated with mealybug wilt of pineapple (Ananas comosus).

Virus Genes 2021 Oct 28;57(5):464-468. Epub 2021 Jun 28.

Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.

Mealybug wilt of pineapple (MWP) is the most important and complex viral disease affecting pineapple worldwide. High-throughput sequencing was conducted to characterize a new virus identified only in symptomatic pineapple plants and tentatively named pineapple mealybug wilt-associated virus 6 (PMWaV-6). Data analyses revealed a genome of 17,854 nucleotides with an organization resembling members of the genus Ampelovirus, family Closteroviridae. Read More

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October 2021

Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms.

Front Microbiol 2021 7;12:694601. Epub 2021 Jun 7.

National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, China.

A novel negative-sense, single-stranded (ss) RNA virus was identified in a "Shennong Jinhuanghou" (SJ) grapevine showing severe chlorotic mottling symptoms by integrating high-throughput sequencing (HTS) and conventional Sanger sequencing of reverse transcription polymerase chain reaction (RT-PCR) products. The virus was provisionally named as "grapevine emaravirus A" (GEVA). GEVA had a genome comprising five genomic RNA segments, each containing a single open reading frame on the viral complementary strand and two untranslated regions with complementary 13- nt stretches at the 5' and 3' terminal ends. Read More

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Small RNA and transcriptome sequencing of a symptomatic peony plant reveals mixed infections with novel viruses.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Shang Qiao Zhong Lu, Chaoyang DistrictBeijing, China, 100024.

To identify the viruses in tree peony plants associated with the symptoms of yellowing, leaf rolling, stunted growth, and decline, high-throughput sequencing of small RNA and mRNA was conducted from a single symptomatic plant. Bioinformatic analyses and reconstruction of viral genomes indicated mixed viral infections involving cycas necrotic stunt virus (CNSV), apple stem grooving virus (ASGV), lychnis mottle virus (LycMoV), grapevine line pattern virus (GLPV), and three new viruses designated as peony yellowing-associated citrivirus (PYaCV, Citrivirus in Betaflexiviridae), peony betaflexivirus 1 (PeV1, unclassified in Betaflexiviridae), and peony leafroll-associated virus (PLRaV, Ampelovirus in Closteroviridae). PYaCV was 8,666 nucleaotides (nt) in length, comprising three open reading frames (ORFs) and shared 63. Read More

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Use of Ground Covers to Control Three-Cornered Alfalfa Hopper, Spissistilus festinus (Hemiptera: Membracidae), and Other Suspected Vectors of Grapevine Red Blotch Virus.

J Econ Entomol 2021 Aug;114(4):1462-1469

Dept. Entomology, University of California - Riverside, 900 University Ave., Riverside, CA 92521, USA.

Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease, which affects wine grapes and leads to reduced crop yield and quality. The pathogen-plant-vector relationship of GRBV is not well understood; however, some possible vectors have been identified: Caladonus coquilletti (Van Duzee; Hemiptera: Cicadellidae), Colladonus reductus (Van Duzee; Hemiptera: Cicadellidae), Erythroneura spp., Melanoliarus sp. Read More

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Polymorphisms at the 3'end of the movement protein (MP) gene of grapevine Pinot gris virus (GPGV) affect virus titre and small interfering RNA accumulation in GLMD disease.

Virus Res 2021 Sep 11;302:198482. Epub 2021 Jun 11.

Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine 33100, Italy; Istituto Nazionale Biostrutture e Biosistemi, Rome, Italy. Electronic address:

Grapevine Leaf Mottling and Deformation (GLMD) is a grapevine disease that has been associated with a trichovirus, the grapevine Pinot gris virus (GPGV). A wide diversity in the severity of GLMD disease symptoms has been recorded worldwide, but the relationship of this diversity to the sequence variation in the GPGV genome is still a matter of debate. Results from comparative analysis of GPGV genomic sequences have suggested an association of polymorphisms at the 3'-end of the movement protein (MP) with GLMD severity. Read More

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September 2021

Selected genotypes with the genetic background of Vitis aestivalis and Vitis labrusca are resistant to Xiphinema index.

Plant Dis 2021 Jun 10. Epub 2021 Jun 10.

Dienstleistungszentrum ländlicher Raum, 111096, Breitenweg 71, Neustadt, Germany, 67435;

The ectoparasitic nematode Xiphinema index transmits grapevine fanleaf virus (GFLV) during feeding on grapevine roots, causing fanleaf degeneration in the plant. Hence, resistance breeding is a key to develop novel rootstocks to overcome such threats. In the past years, various grapevine species were screened, and a few candidates with partial resistance were identified. Read More

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First report of Grapevine yellow speckle viroid 1 infecting grapevine (Vitis vinifera L.) in Canada.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

Agriculture and AgriFood Canada , Research and development center , 430 Gouin, Saint-Jean-sur-Richelieu, Quebec, Canada, J3B3E6;

Quebec is the third largest wine grape producer in Canada in acreage, tonnage, and wine grape sales (Carisse et al. 2017; Ben Moussa et al. 2019). Read More

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Prevalence and genetic diversity of viruses associated with rugose wood complex in Greek vineyards.

Plant Dis 2021 Jun 4. Epub 2021 Jun 4.

Aristotle University of Thessaloniki, School of Agriculture, Plant Pathology Lab, Thessaloniki, Greece, 54124;

Rugose wood is one of the most important disease syndromes of grapevine and it has been associated with at least three viruses: grapevine rupestris stem pitting associated virus (GRSPaV), grapevine virus A (GVA) and grapevine virus B (GVB). All three viruses show a worldwide distribution pattern, and their genetic composition has been the focus of extensive research over the past years. Despite their first record in Greece almost 20 years ago, there is a lack of knowledge on the distribution and genetic variability of their populations in Greek vineyards. Read More

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Rootstock influences the effect of grapevine leafroll-associated viruses on berry development and metabolism via abscisic acid signalling.

Mol Plant Pathol 2021 Aug 1;22(8):984-1005. Epub 2021 Jun 1.

Department of Viticulture and Enology, University of California, Davis, California, USA.

Grapevine leafroll-associated virus (GLRaV) infections are accompanied by symptoms influenced by host genotype, rootstock, environment, and which individual or combination of GLRaVs is present. Using a dedicated experimental vineyard, we studied the responses to GLRaVs in ripening berries from Cabernet Franc grapevines grafted to different rootstocks and with zero, one, or pairs of leafroll infection(s). RNA sequencing data were mapped to a high-quality Cabernet Franc genome reference assembled to carry out this study and integrated with hormone and metabolite abundance data. Read More

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Distribution and Genetic Diversity of Grapevine Viruses in Russia.

Plants (Basel) 2021 May 27;10(6). Epub 2021 May 27.

Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.

Viral diseases can seriously damage the vineyard productivity and the quality of grape and wine products. Therefore, the study of the species composition and range of grapevine viruses is important for the development and implementation of strategies and tactics to limit their spread and increase the economic benefits of viticulture. In 2014-2019, we carried out a large-scale phytosanitary monitoring of Russian commercial vineyards in the Krasnodar region, Stavropol region and Republic of Crimea. Read More

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Bacteriophage-Host Association in the Phytoplasma Insect Vector .

Pathogens 2021 May 17;10(5). Epub 2021 May 17.

Institute for Sustainable Plant Protection, National Research Council of Italy, Strada delle Cacce 73, 10135 Torino, Italy.

Insect vectors transmit viruses and bacteria that can cause severe diseases in plants and economic losses due to a decrease in crop production. Insect vectors, like all other organisms, are colonized by a community of various microorganisms, which can influence their physiology, ecology, evolution, and also their competence as vectors. The important ecological meaning of bacteriophages in various ecosystems and their role in microbial communities has emerged in the past decade. Read More

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Retention and Transmission of Grapevine Leafroll-Associated Virus 3 by .

Front Microbiol 2021 12;12:663948. Epub 2021 May 12.

The New Zealand Institute for Plant and Food Research Limited (PFR), Auckland, New Zealand.

Grapevine leafroll-associated virus 3 (GLRaV-3), an economically significant pathogen of grapevines, is transmitted by , a mealybug commonly found in New Zealand vineyards. To help inform alternative GLRaV-3 control strategies, this study evaluated the three-way interaction between the mealybug, its plant host and the virus. The retention and transmission of GLRaV-3 by after access to non- host plants (and a non-GLRaV-3 host) White clover ( L. Read More

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A single resistance factor to solve vineyard degeneration due to grapevine fanleaf virus.

Commun Biol 2021 05 28;4(1):637. Epub 2021 May 28.

INRAE, Université de Strasbourg, UMR SVQV, Colmar, France.

Grapevine fanleaf disease, caused by grapevine fanleaf virus (GFLV), transmitted by the soil-borne nematode Xiphinema index, provokes severe symptoms and economic losses, threatening vineyards worldwide. As no effective solution exists so far to control grapevine fanleaf disease in an environmentally friendly way, we investigated the presence of resistance to GFLV in grapevine genetic resources. We discovered that the Riesling variety displays resistance to GFLV, although it is susceptible to X. Read More

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Identification of protein interactions of grapevine fanleaf virus RNA-dependent RNA polymerase during infection of by affinity purification and tandem mass spectrometry.

J Gen Virol 2021 05;102(5)

Cornell University, Plant Pathology and Plant Microbe-Biology Section, School of Integrative Plant Science, Cornell AgriTech at the New York State Agricultural Experiment Station, Geneva, NY 14456, USA.

The RNA-dependent RNA polymerase (1E) is involved in replication of grapevine fanleaf virus (GFLV, , ) and causes vein clearing symptoms in . Information on protein 1E interaction with other viral and host proteins is scarce. To study protein 1E biology, three GFLV infectious clones, i. Read More

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Exploring Grower-sourced Data to Understand Spatiotemporal Trends in the Occurrence of a Vector, Pseudococcus maritimus (Hemiptera: Pseudococcidae) and Improve Grapevine Leafroll Disease Management.

J Econ Entomol 2021 Aug;114(4):1452-1461

UC Cooperative Extension, 1710 Soscol Ave, Suite 4, Napa, CA 94559, USA.

Grapevine leafroll disease is a significant concern in the wine grape industry, as it spreads rapidly and contributes to economically significant reductions in yield and grape quality. Our objective was to utilize 5 yr of grower-sourced data from Napa (California, USA) to improve local and regional disease management efforts. Specifically, we applied a spatially integrated multivariate clustering technique to improve understanding of spatiotemporal trends in Pseudococcus maritimus (Ehrhorn) male populations-the primary vector in the region. Read More

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Trigger and suppression of antiviral defenses by grapevine Pinot gris virus (GPGV): novel insights into virus-host interaction.

Mol Plant Microbe Interact 2021 May 13. Epub 2021 May 13.

University of Udine, 9316, Department of Agriculture, Food, Environmental and Animal Sciences, Udine, Italy;

Grapevine Pinot gris virus (GPGV) is an emerging trichovirus that has been putatively associated with a novel grapevine disease known as grapevine leaf mottling and deformation (GLMD). Yet the role of GPGV in GLMD disease is poorly understood since it has been detected both in symptomatic and symptomless grapevines. We exploited a recently constructed GPGV infectious clone (pRI::GPGV-vir) to induce an antiviral response in Nicotiana benthamiana plants. Read More

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Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus.

mBio 2021 05 11;12(3). Epub 2021 May 11.

Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid/Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Pozuelo de Alarcón, Madrid, Spain

is one of the most important plant-pathogenic fungus. Products based on microorganisms can be used in biocontrol strategies alternative to chemical control, and mycoviruses have been explored as putative biological agents in such approaches. Here, we have explored the mycovirome of isolates from grapevine of Italy and Spain to increase the knowledge about mycoviral diversity and evolution, and to search for new widely distributed mycoviruses that could be active ingredients in biological products to control this hazardous fungus. Read More

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Complete genome sequence of a novel citrus virus with characteristics of members of the family Tymoviridae.

Arch Virol 2021 Jul 5;166(7):2055-2058. Epub 2021 May 5.

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.

A novel positive-stranded RNA virus provisionally named "citrus virus C" (CVC) was discovered in citrus trees displaying mottling symptoms. Its genome comprises 7,215 nucleotides (nt), excluding the 3' poly(A) tail, and contains two open reading frames (ORFs) that encode a replication-associated polyprotein (RP) and a putative coat protein (CP). The CVC genome contains a 16-nt 'marafibox', which is highly conserved in most viruses belonging to the genus Marafivirus of the same family. Read More

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