1,259 results match your criteria grapevine genome

Elevated transcription of transposable elements is accompanied by het-siRNA-driven de novo DNA methylation in grapevine embryogenic callus.

BMC Genomics 2021 Sep 20;22(1):676. Epub 2021 Sep 20.

Department Wine, Food and Molecular Biosciences, Lincoln University, Canterbury, New Zealand.

Background: Somatic variation is a valuable source of trait diversity in clonally propagated crops. In grapevine, which has been clonally propagated worldwide for centuries, important phenotypes such as white berry colour are the result of genetic changes caused by transposable elements. Additionally, epiallele formation may play a role in determining geo-specific ('terroir') differences in grapes and thus ultimately in wine. Read More

View Article and Full-Text PDF
September 2021

Discovery of the Locus From for Stable Resistance to Grapevine Powdery Mildew in a Family Segregating for Several Unstable and Tissue-Specific Quantitative Resistance Loci.

Front Plant Sci 2021 3;12:733899. Epub 2021 Sep 3.

School of Integrative Plant Science, Cornell AgriTech, Cornell University, Geneva, NY, United States.

Race-specific resistance loci, whether having qualitative or quantitative effects, present plant-breeding challenges for phenotypic selection and deciding which loci to select or stack with other resistance loci for improved durability. Previously, resistance to grapevine powdery mildew (GPM, caused by ) was predicted to be conferred by at least three race-specific loci in the mapping family B37-28 × C56-11 segregating for GPM resistance from . In this study, 9 years of vineyard GPM disease severity ratings plus a greenhouse and laboratory assays were genetically mapped, using a rhAmpSeq core genome marker platform with 2,000 local haplotype markers. Read More

View Article and Full-Text PDF
September 2021

Identification of C3H2C3-type RING E3 ubiquitin ligase in grapevine and characterization of drought resistance function of VyRCHC114.

BMC Plant Biol 2021 Sep 17;21(1):422. Epub 2021 Sep 17.

College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.

Background: RING is one of the largest E3 ubiquitin ligase families and C3H2C3 type is the largest subfamily of RING, which plays an important role in plant growth and development, and growth and responses to biotic and abiotic stresses.

Results: A total of 143 RING C3H2C3-type genes (RCHCs) were discovered from the grapevine genome and separated into groups (I-XI) according to their phylogenetic analysis, and these genes named according to their positions on chromosomes. Gene replication analysis showed that tandem duplications play a predominant role in the expansion of VvRCHCs family together. Read More

View Article and Full-Text PDF
September 2021

Introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptation.

Genome Biol 2021 09 3;22(1):254. Epub 2021 Sep 3.

Department of Ecology and Evolutionary Biology, University of California Irvine, Irvine, CA, USA.

Background: Introgressive hybridization can reassort genetic variants into beneficial combinations, permitting adaptation to new ecological niches. To evaluate evolutionary patterns and dynamics that contribute to introgression, we investigate six wild Vitis species that are native to the Southwestern United States and useful for breeding grapevine (V. vinifera) rootstocks. Read More

View Article and Full-Text PDF
September 2021

Gimme shelter: three-dimensional architecture of the endoplasmic reticulum, the replication site of grapevine Pinot gris virus.

Funct Plant Biol 2021 Sep;48(10):1074-1085

Department of Agriculture, Food, Environmental and Animal Sciences, University of Udine,via delle Scienze, 206, 33100 - Udine, Italy; and Corresponding author. Email:

Grapevine leaf mottling and deformation is a novel grapevine disease that has been associated with grapevine Pinot gris virus (GPGV). The virus was observed exclusively inside membrane-bound structures in the bundle sheath cells of the infected grapevines. As reported widely in the literature, many positive-sense single-stranded RNA viruses modify host-cell membranes to form a variety of deformed organelles, which shelter viral genome replication from host antiviral compounds. Read More

View Article and Full-Text PDF
September 2021

Seeing the Forest through the (Phylogenetic) Trees: Functional Characterisation of Grapevine Terpene Synthase () Paralogues and Orthologues.

Plants (Basel) 2021 Jul 26;10(8). Epub 2021 Jul 26.

Department of Viticulture and Oenology, Faculty of AgriSciences, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch 7600, South Africa.

Gene families involved in specialised metabolism play a key role in a myriad of ecophysiological and biochemical functions. The sesquiterpene synthases represent the largest subfamily of grapevine terpene synthase () genes and are important volatile metabolites for wine flavour and aroma, as well as ecophysiological interactions. The functional characterisation of genes is complicated by a reliance on a single reference genome that greatly underrepresents this large gene family, exacerbated by extensive duplications and paralogy. Read More

View Article and Full-Text PDF

Genome Sequence Resource for Erysiphe necator NAFU1, a Grapevine Powdery Mildew Isolate Identified in Shaanxi Province of China.

Mol Plant Microbe Interact 2021 Aug 26. Epub 2021 Aug 26.

Northwest A&F University, 12469, State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Yangling, Shaanxi, China.

Erysiphe necator is an economically important biotrophic fungal pathogen responsible for powdery mildew disease on grapevine. Currently, genome sequences are available for only a few Erysiphe necator isolates from USA. Based on the combination of Nanopore and Illumina sequencing technologies, we present here the complete genome assembly for an isolate of E. Read More

View Article and Full-Text PDF

First report of Grapevine enamovirus 1 in Vitis vinifera cultivar 'Meunier' in France.

Plant Dis 2021 Aug 22. Epub 2021 Aug 22.

Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France, Colmar, France;

Grapevine enamovirus 1 (GEV-1) is a member of the genus Enamovirus in the family Solemoviridae. GEV-1 was first described in 2017 in a few grapevine cultivars in Brazil (Silva et al. 2017) and subsequently in China (Ren et al. Read More

View Article and Full-Text PDF

High-Throughput Sequencing Indicates a Novel Marafivirus in Grapevine Showing Vein-Clearing Symptoms.

Plants (Basel) 2021 Jul 20;10(7). Epub 2021 Jul 20.

National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.

A putative new marafivirus was identified in a 'Jumeigui' grapevine exhibitting obvious vein-clearing symptoms by high-throughput sequencing, which tentatively named grapevine-associated marafivirus (GaMV). The nearly complete genomic sequence of GaMV was amplified by reverse transcription PCR, and the terminal sequences were determined using the rapid amplification of cDNA ends method. The nearly complete genome of GaMV is 6346 bp long, excluding the poly(A) tail, and shows 51. Read More

View Article and Full-Text PDF

Metagenomic analysis of nepoviruses: diversity, evolution and identification of a genome region in members of subgroup A that appears to be important for host range.

Arch Virol 2021 Oct 9;166(10):2789-2801. Epub 2021 Aug 9.

Université de Strasbourg, INRAE, SVQV, UMR-A 1131, F-68000, Colmar, France.

Data mining and metagenomic analysis of 277 open reading frame sequences of bipartite RNA viruses of the genus Nepovirus, family Secoviridae, were performed, documenting how challenging it can be to unequivocally assign a virus to a particular species, especially those in subgroups A and C, based on some of the currently adopted taxonomic demarcation criteria. This work suggests a possible need for their amendment to accommodate pangenome information. In addition, we revealed a host-dependent structure of arabis mosaic virus (ArMV) populations at a cladistic level and confirmed a phylogeographic structure of grapevine fanleaf virus (GFLV) populations. Read More

View Article and Full-Text PDF
October 2021

Conservation of endo-glucanase 16 (EG16) activity across highly divergent plant lineages.

Biochem J 2021 Aug;478(16):3063-3078

Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC Canada V6T 1Z1.

Plant cell walls are highly dynamic structures that are composed predominately of polysaccharides. As such, endogenous carbohydrate active enzymes (CAZymes) are central to the synthesis and subsequent modification of plant cells during morphogenesis. The endo-glucanase 16 (EG16) members constitute a distinct group of plant CAZymes, angiosperm orthologs of which were recently shown to have dual β-glucan/xyloglucan hydrolase activity. Read More

View Article and Full-Text PDF

CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera).

Hortic Res 2020 Aug 1;7(1):116. Epub 2020 Aug 1.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Grapevine (Vitis vinifera), one of the most economically important fruit crops in the world, suffers significant yield losses from powdery mildew, a major fungal disease caused by Erysiphe necator. In addition to suppressing host immunity, phytopathogens modulate host proteins termed susceptibility (S) factors to promote their proliferation in plants. In this study, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) technology was used to enable the targeted mutagenesis of MLO (mildew resistance Locus O) family genes that are thought to serve as S factors for powdery mildew fungi. Read More

View Article and Full-Text PDF

Bacteria associated with wood tissues of Esca-diseased grapevines: functional diversity and synergy with Fomitiporia mediterranea to degrade wood components.

Environ Microbiol 2021 Jul 20. Epub 2021 Jul 20.

INRAE, UMR SAVE, Bordeaux Science Agro, ISVV, University of Bordeaux, Villenave d'Ornon, 33882, France.

Fungi are considered to cause grapevine trunk diseases such as esca that result in wood degradation. For instance, the basidiomycete Fomitiporia mediterranea (Fmed) is overabundant in white rot, a key type of wood-necrosis associated with esca. However, many bacteria colonize the grapevine wood too, including the white rot. Read More

View Article and Full-Text PDF

Data Comparison and Software Design for Easy Selection and Application of CRISPR-based Genome Editing Systems in Plants.

Genomics Proteomics Bioinformatics 2021 Jul 16. Epub 2021 Jul 16.

Beijing Key Laboratory of Grape Science and Enology, and CAS Key Laboratory of Plant Resources, Institute of Botany, the Innovative Academy of Seed Design, Chinese Academy of Science, Beijing 100093, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China. Electronic address:

CRISPR-based genome editing systems have been successfully and effectively used in many organisms. However, only a few studies have reported the comparison between CRISPR/Cas9 and CRISPR/Cpf1 systems in the whole-genome applications. Although many web-based toolkits are available, there is still a shortage of comprehensive, user-friendly, and plant-specific CRISPR databases and desktop software. Read More

View Article and Full-Text PDF

Detoxification gene families in Phylloxera: Endogenous functions and roles in response to the environment.

Comp Biochem Physiol Part D Genomics Proteomics 2021 Jun 25;40:100867. Epub 2021 Jun 25.

Université Côte d'Azur, INRAE, CNRS, ISA, 400 Route des Chappes, 06903 Sophia Antipolis, France. Electronic address:

Phylloxera, Daktulosphaira vitifoliae, is an agronomic pest that feeds monophagously on grapevine, Vitis spp. host plants. Phylloxera manipulates primary and secondary plant metabolism to establish either leaf or root galls. Read More

View Article and Full-Text PDF

Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time.

BMC Plant Biol 2021 Jul 7;21(1):327. Epub 2021 Jul 7.

Genetics and Genomics of Plants, Faculty of Biology & Center for Biotechnology, Bielefeld University, Bielefeld, Germany.

Background: Grapevine cultivars of the Pinot family represent clonally propagated mutants with major phenotypic and physiological differences, such as different colour or shifted ripening time, as well as changes in important viticultural traits. Specifically, the cultivars 'Pinot Noir' (PN) and 'Pinot Noir Precoce' (PNP, early ripening) flower at the same time, but vary in the beginning of berry ripening (veraison) and, consequently, harvest time. In addition to genotype, seasonal climatic conditions (i. Read More

View Article and Full-Text PDF

First report of grapevine rupestris vein feathering virus in L. from Japan.

Plant Dis 2021 Jul 7. Epub 2021 Jul 7.

University of Yamanashi, 38146, The Institute of Enology and Viticulture, Kofu, Yamanashi, Japan;

The production of wine grapes is gaining widespread popularity and being carried out on approximately 2,200 hectares of land in Japan. Scions grafted onto rootstocks generally have been imported from the EU, USA, New Zealand, and Australia into Japan. Unfortunately, viruses have spread in Japanese vineyards by slipping through the net of plant quarantine. Read More

View Article and Full-Text PDF

First Report of Grapevine Kizil Sapak Virus in Grapevine in China.

Plant Dis 2021 Jul 6. Epub 2021 Jul 6.

National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, China;

Grapevine Kizil Sapak virus (GKSV) is a novel member of the family Betaflexiviridae classified into the proposed genus Fivivirus within the subfamily Trivirinae. It was first discovered in USA from a grapevine originating from Turkmenistan (Al Rwahnih et al. 2019) and later in France from a grapevine accession from Iran (Marais et al. Read More

View Article and Full-Text PDF

Open chromatin in grapevine marks candidate CREs and with other chromatin features correlates with gene expression.

Plant J 2021 Jul 5. Epub 2021 Jul 5.

Dipartimento di Scienze Agroalimentari, Ambientali e Animali (DI4A), Udine, I-33100, Italy.

Vitis vinifera is an economically important crop and a useful model in which to study chromatin dynamics. In contrast to the small and relatively simple genome of Arabidopsis thaliana, grapevine contains a complex genome of 487 Mb that exhibits extensive colonization by transposable elements. We used Hi-C, ChIP-seq and ATAC-seq to measure how chromatin features correlate to the expression of 31 845 grapevine genes. Read More

View Article and Full-Text PDF

Genome-Wide Association Study Reveals Genomic Region Associated with Mite-Recruitment Phenotypes in the Domesticated Grapevine ().

Genes (Basel) 2021 Jun 30;12(7). Epub 2021 Jun 30.

Department of Plant Biology, Program in Ecology, Evolution, and Behavior, Michigan State University, East Lansing, MI 48824, USA.

Indirect defenses are plant phenotypes that reduce damage by attracting natural enemies of plant pests and pathogens to leaves. Despite their economic and ecological importance, few studies have investigated the genetic underpinnings of indirect defense phenotypes. Here, we present a genome-wide association study of five phenotypes previously determined to increase populations of beneficial (fungivorous and predacious) mites on grape leaves (genus ): leaf bristles, leaf hairs, and the size, density, and depth of leaf domatia. Read More

View Article and Full-Text PDF

Beneficial Microorganisms to Control the Gray Mold of Grapevine: From Screening to Mechanisms.

Microorganisms 2021 Jun 25;9(7). Epub 2021 Jun 25.

Université de Reims Champagne-Ardenne, RIBP EA4707 USC INRAE 1488, SFR Condorcet FR CNRS 3417, 51100 Reims, France.

In many vineyards around the world, () causes one of the most serious diseases of aerial grapevine (.) organs. The control of the disease relies mainly on the use of chemical products whose use is increasingly challenged. Read More

View Article and Full-Text PDF

Variable Populations of Grapevine Virus T Are Present in Vineyards of Hungary.

Viruses 2021 06 10;13(6). Epub 2021 Jun 10.

Institute of Plant Protection, Hungarian University of Agriculture and Life Sciences, Ménesi Road 44, H-1118 Budapest, Hungary.

Grapevine virus T (GVT) is a recently described foveavirus, which was identified from a transcriptome of a Teroldego grapevine cultivar in 2017. Recently, we surveyed vineyards and rootstock plantations in Hungary using small RNA (sRNA) high-throughput sequencing (HTS), at a time when GVT had not yet been described. A re-analysis of our sRNA HTS datasets and a survey of grapevines by RT-PCR revealed the presence of GVT in most of the vineyards tested, while at rootstock fields its presence was very rare. Read More

View Article and Full-Text PDF

A novel ampelovirus associated with mealybug wilt of pineapple (Ananas comosus).

Virus Genes 2021 Oct 28;57(5):464-468. Epub 2021 Jun 28.

Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.

Mealybug wilt of pineapple (MWP) is the most important and complex viral disease affecting pineapple worldwide. High-throughput sequencing was conducted to characterize a new virus identified only in symptomatic pineapple plants and tentatively named pineapple mealybug wilt-associated virus 6 (PMWaV-6). Data analyses revealed a genome of 17,854 nucleotides with an organization resembling members of the genus Ampelovirus, family Closteroviridae. Read More

View Article and Full-Text PDF
October 2021

Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms.

Front Microbiol 2021 7;12:694601. Epub 2021 Jun 7.

National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, China.

A novel negative-sense, single-stranded (ss) RNA virus was identified in a "Shennong Jinhuanghou" (SJ) grapevine showing severe chlorotic mottling symptoms by integrating high-throughput sequencing (HTS) and conventional Sanger sequencing of reverse transcription polymerase chain reaction (RT-PCR) products. The virus was provisionally named as "grapevine emaravirus A" (GEVA). GEVA had a genome comprising five genomic RNA segments, each containing a single open reading frame on the viral complementary strand and two untranslated regions with complementary 13- nt stretches at the 5' and 3' terminal ends. Read More

View Article and Full-Text PDF

Small RNA and transcriptome sequencing of a symptomatic peony plant reveals mixed infections with novel viruses.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Shang Qiao Zhong Lu, Chaoyang DistrictBeijing, China, 100024.

To identify the viruses in tree peony plants associated with the symptoms of yellowing, leaf rolling, stunted growth, and decline, high-throughput sequencing of small RNA and mRNA was conducted from a single symptomatic plant. Bioinformatic analyses and reconstruction of viral genomes indicated mixed viral infections involving cycas necrotic stunt virus (CNSV), apple stem grooving virus (ASGV), lychnis mottle virus (LycMoV), grapevine line pattern virus (GLPV), and three new viruses designated as peony yellowing-associated citrivirus (PYaCV, Citrivirus in Betaflexiviridae), peony betaflexivirus 1 (PeV1, unclassified in Betaflexiviridae), and peony leafroll-associated virus (PLRaV, Ampelovirus in Closteroviridae). PYaCV was 8,666 nucleaotides (nt) in length, comprising three open reading frames (ORFs) and shared 63. Read More

View Article and Full-Text PDF

Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes.

BMC Plant Biol 2021 Jun 19;21(1):279. Epub 2021 Jun 19.

College of Horticulture, Nanjing Agricultural University, Jiangsu Province, Nanjing City, 210095, PR China.

Background: 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a key enzyme in the mevalonate (MVA) pathway, which regulates the metabolism of terpenoids in the cytoplasm and determines the type and content of downstream terpenoid metabolites.

Results: Results showed that grapevine HMGR family has three members, such as VvHMGR1, VvHMGR2, and VvHMGR3. The expression of VvHMGRs in 'Kyoho' has tissue specificity, for example, VvHMGR1 keeps a higher expression, VvHMGR2 is the lowest, and VvHMGR3 gradually decreases as the fruit development. Read More

View Article and Full-Text PDF

Polymorphisms at the 3'end of the movement protein (MP) gene of grapevine Pinot gris virus (GPGV) affect virus titre and small interfering RNA accumulation in GLMD disease.

Virus Res 2021 Sep 11;302:198482. Epub 2021 Jun 11.

Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine 33100, Italy; Istituto Nazionale Biostrutture e Biosistemi, Rome, Italy. Electronic address:

Grapevine Leaf Mottling and Deformation (GLMD) is a grapevine disease that has been associated with a trichovirus, the grapevine Pinot gris virus (GPGV). A wide diversity in the severity of GLMD disease symptoms has been recorded worldwide, but the relationship of this diversity to the sequence variation in the GPGV genome is still a matter of debate. Results from comparative analysis of GPGV genomic sequences have suggested an association of polymorphisms at the 3'-end of the movement protein (MP) with GLMD severity. Read More

View Article and Full-Text PDF
September 2021

Investigation of long non-coding RNAs as regulatory players of grapevine response to powdery and downy mildew infection.

BMC Plant Biol 2021 Jun 8;21(1):265. Epub 2021 Jun 8.

Department of Biotechnology, Panjab University, BMS Block I, Sector 25, Chandigarh, 160014, India.

Background: Long non-coding RNAs (lncRNAs) are regulatory transcripts of length > 200 nt. Owing to the rapidly progressing RNA-sequencing technologies, lncRNAs are emerging as considerable nodes in the plant antifungal defense networks. Therefore, we investigated their role in Vitis vinifera (grapevine) in response to obligate biotrophic fungal phytopathogens, Erysiphe necator (powdery mildew, PM) and Plasmopara viticola (downy mildew, DM), which impose huge agro-economic burden on grape-growers worldwide. Read More

View Article and Full-Text PDF

First report of Grapevine yellow speckle viroid 1 infecting grapevine (Vitis vinifera L.) in Canada.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

Agriculture and AgriFood Canada , Research and development center , 430 Gouin, Saint-Jean-sur-Richelieu, Quebec, Canada, J3B3E6;

Quebec is the third largest wine grape producer in Canada in acreage, tonnage, and wine grape sales (Carisse et al. 2017; Ben Moussa et al. 2019). Read More

View Article and Full-Text PDF

Current status and future prospects of grapevine anthracnose caused by Elsinoe ampelina: An important disease in humid grape-growing regions.

Mol Plant Pathol 2021 Aug 7;22(8):899-910. Epub 2021 Jun 7.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China.

Anthracnose, caused by Elsinoe ampelina, is one of the most destructive diseases of grapevines worldwide, especially in humid areas. E. ampelina mainly infects young tissues starting from shoots to berries and affects vine vigour and berry yield. Read More

View Article and Full-Text PDF