2,935 results match your criteria graft vascularization


Insulin expression and C-peptide in type 1 diabetes subjects implanted with stem cell-derived pancreatic endoderm cells in an encapsulation device.

Cell Rep Med 2021 Dec 2;2(12):100466. Epub 2021 Dec 2.

ViaCyte Inc., San Diego, CA 92121, USA.

These preliminary data from an ongoing first-in-human phase 1/2, open-label study provide proof-of-concept that pluripotent stem cell-derived pancreatic endoderm cells (PEC-01) engrafted in type 1 diabetes patients become islet cells releasing insulin in a physiologically regulated fashion. In this study of 17 subjects aged 22-57 with type 1 diabetes, PEC-01 cells were implanted subcutaneously in VC-02 macroencapsulation devices, allowing for direct vascularization of the cells. Engraftment and insulin expression were observed in 63% of VC-02 units explanted from subjects at 3-12 months post-implant. Read More

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December 2021

Optimization of Adipose Tissue Cryopreservation Techniques in a Murine Model.

Plast Reconstr Surg Glob Open 2021 Nov 11;9(11):e3926. Epub 2021 Nov 11.

Unidad de Genética, Hospital Universitario y Politécnico La Fe, Valencia, Spain.

Background: The aim of this study was to develop an adipose tissue (AT) cryopreservation protocol that is effective, simple, and maintains the functionality and viability of AT after thawing and transplantation.

Methods: Two cryopreservation temperatures (T°), -20°C and -80°C, and two cryoprotective agents (CPAs), trehalose and hydroxyethyl starch (HES), were compared first in an experimental study, using a slowfreezing protocol. The five experimental groups were the following: (a) Fresh AT (control group), (b) T = -20°C, 10%HES, (c) T = -80°C, 10%HES, (d) T = -20°C, 0. Read More

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November 2021

Biomaterials assisted reconstructive urology: The pursuit of an implantable bioengineered neo-urinary bladder.

Biomaterials 2021 Dec 30;281:121331. Epub 2021 Dec 30.

Laboratory for Biomaterials, Materials Research Centre, Indian Institute of Science, Bangalore, 560012, India; Centre for Biosystems Science and Engineering, Indian Institute of Science, Bangalore, 560012, India. Electronic address:

Urinary bladder is a dynamic organ performing complex physiological activities. Together with ureters and urethra, it forms the lower urinary tract that facilitates urine collection, low-pressure storage, and volitional voiding. However, pathological disorders are often liable to cause irreversible damage and compromise the normal functionality of the bladder, necessitating surgical intervention for a reconstructive procedure. Read More

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December 2021

Engineering Vascularizing Electrospun Dermal Grafts by Integrating Fish Collagen and Ion-Doped Bioactive Glass.

ACS Biomater Sci Eng 2022 Jan 11. Epub 2022 Jan 11.

Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata 700037, India.

Utilizing bioactive molecules from organic sources in combination with inorganic materials for enhanced tissue regeneration has been a focus of recent scientific advancements. Some recent studies showed the potential of some specialized bioactive glass for healing of soft tissues; the role of Rohu () skin-derived collagen, a biopolymer in tissue regeneration and cutaneous healing, is yet to be established. So, we have fabricated four different types of electrospun mats as wound dressing materials/dermal grafts by combining locally sourced fish (Rohu) skin-derived collagen with novel composition of bioactive glass (Fcol/BAG) without and with dopants (3% and 5% Cu and Co, respectively and their binary) aimed at achieving an accelerated wound healing. Read More

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January 2022

Degradable methacrylic acid-based synthetic hydrogel for subcutaneous islet transplantation.

Biomaterials 2021 Dec 30;281:121342. Epub 2021 Dec 30.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, M5S 3E5, Canada; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, Ontario, M5S 3G9, Canada. Electronic address:

Islet transplantation is a promising regenerative therapy that would reduce the dependence of type 1 diabetic patients on insulin injections. However, islet transplantation is not yet widely available, in part because there is no ideal transplant site. The subcutaneous space has been highlighted as a promising transplant site, but it does not have the vasculature required to support an islet graft. Read More

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December 2021

Platelet-Rich Fibrin Used in Regenerative Endodontics and Dentistry: Current Uses, Limitations, and Future Recommendations for Application.

Int J Dent 2021 15;2021:4514598. Epub 2021 Dec 15.

Conservative Dentistry Unit, School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia.

Regenerative endodontics has introduced numerous procedures such as pulp implantation, revascularization, and postnatal stem cell therapy. Revascularization has been successfully implemented clinically nowadays, thus providing dentists with outrageous results. Platelet-rich fibrin (PRF) used either alone or along with bone graft promotes bone growth and vascularization. Read More

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December 2021

Surgical outcomes of concomitantly performed penetrating keratoplasty with intrascleral haptic fixation.

Indian J Ophthalmol 2022 Jan;70(1):100-106

Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.

Purpose: : To describe the surgical results of concomitantly performed optical penetrating keratoplasty (PKP) with glued intrascleral haptic fixation (ISHF).

Methods: : Retrospective review of 18 patients (15-72 years) with best-corrected visual acuity (BCVA) of ≤1/60 subjected to unilateral concomitant optical PKP with ISHF and followed up for 13.11 ± 5. Read More

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January 2022

Corneal Sarcoidosis: Diffuse Stromal Granulomatous Inflammation in a Patient With Sarcoidosis.

Cornea 2021 Dec 22. Epub 2021 Dec 22.

Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD. Dr. Shifera is now with the private practice in Zephyrhills, FL.

Purpose: The purpose of this study was to present a case of diffuse noncaseating granulomas involving the corneal stroma in a patient with ocular and pulmonary sarcoidosis.

Methods: This was a single case report.

Results: A 31-year-old female patient presented with a 6-year history of panuveitis of the right eye along with a history of pulmonary sarcoidosis and a conjunctival biopsy of the right eye that was reported as positive for sarcoidosis. Read More

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December 2021

Emerging strategies for beta cell transplantation to treat diabetes.

Trends Pharmacol Sci 2021 Dec 6. Epub 2021 Dec 6.

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA; Department of Surgery, Houston Methodist Hospital, Houston, TX 77030, USA; Department of Radiation Oncology, Houston Methodist Hospital, Houston, TX 77030, USA. Electronic address:

Beta cell replacement has emerged as an attractive therapeutic alternative to traditional exogenous insulin administration for management of type 1 diabetes (T1D). Beta cells deliver insulin dynamically based on individual glycometabolic requirements, providing glycemic control while significantly reducing patient burden. Although transplantation into the portal circulation is clinically available, poor engraftment, low cell survival, and immune rejection have sparked investigation of alternative strategies for beta cell transplantation. Read More

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December 2021

Integrating engineered macro vessels with self-assembled capillaries in 3D implantable tissue for promoting vascular integration in-vivo.

Biomaterials 2022 Jan 30;280:121286. Epub 2021 Nov 30.

Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Israel. Electronic address:

A functional multi-scale vascular network can promote 3D engineered tissue growth and improve transplantation outcome. In this work, by using a combination of living cells, biological hydrogel, and biodegradable synthetic polymer we fabricated a biocompatible, multi-scale vascular network (MSVT) within thick, implantable engineered tissues. Using a templating technique, macro-vessels were patterned in a 3D biodegradable polymeric scaffold seeded with endothelial and support cells within a collagen gel. Read More

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January 2022

Failed Acellular Nerve Allografts: A Critical Review.

Ann Plast Surg 2021 Dec 3. Epub 2021 Dec 3.

From the Department of Peripheral Nerve Surgery, Queen Elizabeth Hospital HaPPeN Research Fellow, Institute of Translational Medicine Department of Cellular Pathology The Birmingham Peripheral Nerve Injury Service, Queen Elizabeth Hospital, Birmingham, United Kingdom.

Background: Acellular nerve allograft (ANA) occupies an increasingly prominent role in the treatment of peripheral nerve reconstruction. There is demonstrable efficacy; however, some grafts fail to support axonal regrowth and the reasons for this are unclear. This study examines the ANA experience in a specialized peripheral nerve surgery department to discuss the clinical and histological findings in failed cases. Read More

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December 2021

Repair of peripheral nerve injuries using a prevascularized cell-based tissue-engineered nerve conduit.

Biomaterials 2022 Jan 23;280:121269. Epub 2021 Nov 23.

LOEX, Centre de recherche du CHU de Québec-Université Laval, Quebec City, Quebec, Canada; Department of Surgery, Faculty of Medicine, Université Laval, Quebec City, Quebec, Canada. Electronic address:

One of the major challenges in the development of a larger and longer nerve conduit for peripheral nerve repair is the limitation in oxygen and nutrient diffusion within the tissue after transplantation preventing Schwann cell and axonal migration. This restriction is due to the slow neovascularization process of the graft starting from both nerve endings. To overcome this limitation, we propose the design of a living tissue-engineered nerve conduit made of an internal tube with a three-dimensional structure supporting axonal migration, which is inserted inside a hollow external tube that plays the role of an epineurium and is strong enough to be stitched to the severed nerve stumps. Read More

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January 2022

[A prospective randomized controlled study of the effectiveness of artificial dermis combined with split-thickness skin for repairing wounds with bone and tendon exposure in hands and feet].

Zhonghua Shao Shang Za Zhi 2021 Dec;37(12):1130-1136

Department of Burns, Zhengzhou First People's Hospital, Zhengzhou 450004, China.

To explore the clinical effects of artificial dermis combined with split-thickness skin for repairing wounds with bone and tendon exposure in hands and feet. A prospective randomized controlled study was conducted. From October 2018 to February 2020, 82 patients with bone and tendon exposed wounds in hands and feet admitted to the Department of Burns of Zhengzhou First People's Hospital who met the inclusion criteria were selected. Read More

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December 2021

Prefabricated 3D-Printed Tissue-Engineered Bone for Mandibular Reconstruction: A Preclinical Translational Study in Primate.

ACS Biomater Sci Eng 2021 Dec 22;7(12):5727-5738. Epub 2021 Nov 22.

Department of Oral and Maxillofacial Surgery, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou 510182, China.

The advent of three dimensionally (3D) printed customized bone grafts using different biomaterials has enabled repairs of complex bone defects in various in vivo models. However, studies related to their clinical translations are truly limited. Herein, 3D printed poly(lactic--glycolic acid)/β-tricalcium phosphate (PLGA/TCP) and TCP scaffolds with or without recombinant bone morphogenetic protein -2 (rhBMP-2) coating were utilized to repair primate's large-volume mandibular defects and compared efficacy of prefabricated tissue-engineered bone (PTEB) over direct implantation (without prefabrication). Read More

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December 2021

Ovarian transplantation with robotic surgery and a neovascularizing human extracellular matrix scaffold: a case series in comparison to meta-analytic data.

Fertil Steril 2022 Jan 17;117(1):181-192. Epub 2021 Nov 17.

Division of Biostatistics, Department of Public Health Sciences, University of California, Davis, California.

Objective: To report our experience with robot-assisted (RA) autologous cryopreserved ovarian tissue transplantation (ACOTT) with the use of a neovascularizing extracellular matrix scaffold.

Design: Case series with meta-analytic update.

Setting: Academic. Read More

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January 2022

Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis.

Front Bioeng Biotechnol 2021 19;9:756755. Epub 2021 Oct 19.

Division of Transplantation and Regenerative Medicine, Tohoku University, Sendai, Japan.

Whole-organ decellularization is an attractive approach for three-dimensional (3D) organ engineering. However, progress with this approach is hindered by intra-vascular blood coagulation that occurs after implantation of the re-cellularized scaffold, resulting in a short-term graft survival. In this study, we explored an alternative approach for 3D organ engineering through an axial pre-vascularization approach and examined its suitability for pancreatic islet transplantation. Read More

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October 2021

Microvessels enhance vascularization and function of transplanted insulin-producing cells.

Cell Metab 2021 Nov;33(11):2103-2105

Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Electronic address:

Transplantation of insulin-producing cells is an emerging treatment for type 1 diabetes. A recent report in Cell Stem Cell (Aghazadeh et al., 2021) outlines a new approach that accelerates the engraftment and improves the survival and function of such cell transplants by mixing adipose tissue-derived ready-made microvessels with human pancreatic progenitor cells or cadaveric islets prior to transplantation. Read More

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November 2021

A New Surgical Technique and Clinical Outcomes of Operated Proximal Metadiaphyseal Humeral Fractures and Nonunions With the Use of Reversed Anatomic Distal Femoral Locking Plate.

Cureus 2021 Sep 27;13(9):e18309. Epub 2021 Sep 27.

School of Medicine-1st Orthopaedic Department, Aristotle University of Thessaloniki, Thessaloniki, GRC.

Introduction Proximal humeral fractures complicated with metaphyseal and diaphyseal extension are usually treated operatively with 3.5 mm long anatomic proximal humerus plates. However, frequently these comminuted and segmental fracture types may be associated with delayed union, nonunion, and/or plate failure. Read More

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September 2021

Matured Myofibers in Bioprinted Constructs with In Vivo Vascularization and Innervation.

Gels 2021 Oct 15;7(4). Epub 2021 Oct 15.

ARC Centre of Excellence for Electro Materials Science, University of Melbourne, Melbourne, VIC 3010, Australia.

For decades, the study of tissue-engineered skeletal muscle has been driven by a clinical need to treat neuromuscular diseases and volumetric muscle loss. The in vitro fabrication of muscle offers the opportunity to test drug-and cell-based therapies, to study disease processes, and to perhaps, one day, serve as a muscle graft for reconstructive surgery. This study developed a biofabrication technique to engineer muscle for research and clinical applications. Read More

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October 2021

[Experimental study on the effect of three-dimensional porous structures on the vascularization rate of artificial dermis].

Zhonghua Shao Shang Za Zhi 2021 Oct;37(10):959-969

Shenzhen Lando Biomaterials Co., Ltd., Shenzhen Engineering Research Center of Implantable Medical Polymer, Guangdong Engineering Research Center of Implantable Medical Polymer, Shenzhen 518107, China.

To explore the effects of orienting three-dimensional porous network (type A) and honeycomb briquette-shaped vertically penetrating three-dimensional porous network (type B) on the vascularization rate of artificial dermis. The experimental research method was used. The artificial dermis was composed of a double layer of silicone layer and scaffold layer. Read More

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October 2021

Advances in Skin Wound and Scar Repair by Polymer Scaffolds.

Molecules 2021 Oct 10;26(20). Epub 2021 Oct 10.

State Key Laboratory of New Textile Materials and Advanced Processing Technologies, School of Textile Science and Engineering, Wuhan Textile University, Wuhan 430200, China.

Scars, as the result of abnormal wound-healing response after skin injury, may lead to loss of aesthetics and physical dysfunction. Current clinical strategies, such as surgical excision, laser treatment, and drug application, provide late remedies for scarring, yet it is difficult to eliminate scars. In this review, the functions, roles of multiple polymer scaffolds in wound healing and scar inhibition are explored. Read More

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October 2021

Reply: Quality and Quantity-Cultured Human Mononuclear Cells Improve Human Fat Graft Vascularization and Survival in an In Vivo Murine Experimental Model.

Plast Reconstr Surg 2021 12;148(6):1059e-1060e

Department of Plastic and Reconstructive Surgery, Department of Regenerative Therapy, Juntendo University School of Medicine, Tokyo, Japan.

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December 2021

Quality and Quantity-Cultured Human Mononuclear Cells Improve Human Fat Graft Vascularization and Survival in an In Vivo Murine Experimental Model.

Plast Reconstr Surg 2021 12;148(6):1058e-1059e

Plastic Surgery Hospital (Institute), Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

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December 2021

Quality and Quantity-Cultured Human Mononuclear Cells Improve Human Fat Graft Vascularization and Survival in an In Vivo Murine Experimental Model.

Plast Reconstr Surg 2021 12;148(6):1055e-1056e

Department of Plastic and Reconstructive Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and, Peking Union Medical College, Beijing, People's Republic of China.

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December 2021

Reply: Quality and Quantity-Cultured Human Mononuclear Cells Improve Human Fat Graft Vascularization and Survival in an In Vivo Murine Experimental Model.

Plast Reconstr Surg 2021 12;148(6):1056e-1058e

Department of Plastic and Reconstructive Surgery, Department of Regenerative Therapy, Juntendo University School of Medicine, Tokyo, Japan.

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December 2021

Biomechanics of vascular areas of the human cranial dura mater.

J Mech Behav Biomed Mater 2022 01 30;125:104866. Epub 2021 Sep 30.

Department of Anatomy, University of Otago, Dunedin, New Zealand; Institute of Legal Medicine, University of Leipzig, Leipzig, Germany; Institute of Legal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. Electronic address:

Accurate biomechanical properties of the human cranial dura mater are paramount for computational head models, artificial graft developments and biomechanical basic research. Yet, it is unclear whether areas of the dura containing meningeal vessels biomechanically differ from avascular areas. Here, 244 dura mater samples with or without vessels from 32 cadavers were tested in a quasi-static uniaxial tensile testing setup. Read More

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January 2022

Histologic Analysis of Porcine Dermal Graft Augmentation in Treatment of Rotator Cuff Tears.

Am J Sports Med 2021 11 15;49(13):3680-3686. Epub 2021 Oct 15.

Department of Orthopaedic Surgery, Duke University Medical Center, Duke Sports Sciences Institute, Durham, North Carolina, USA.

Background: Biologic augmentation via extracellular matrix (ECM) scaffolds has been utilized to address rotator cuff tears with poor-quality tissue.

Purpose: To evaluate the cellular changes in graft explants taken from patients treated with porcine dermal grafts for rotator cuff tears.

Study Design: Case series; Level of evidence, 4. Read More

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November 2021

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes.

Front Endocrinol (Lausanne) 2021 13;12:732431. Epub 2021 Sep 13.

Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, United States.

Diabetes is a complex disease that affects over 400 million people worldwide. The life-long insulin injections and continuous blood glucose monitoring required in type 1 diabetes (T1D) represent a tremendous clinical and economic burdens that urges the need for a medical solution. Pancreatic islet transplantation holds great promise in the treatment of T1D; however, the difficulty in regulating post-transplantation immune reactions to avoid both allogenic and autoimmune graft rejection represent a bottleneck in the field of islet transplantation. Read More

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September 2021

Angiogenic CD34+CD146+ adipose-derived stromal cells augment recovery of soft tissue after radiotherapy.

J Tissue Eng Regen Med 2021 12 5;15(12):1105-1117. Epub 2021 Oct 5.

Department of Surgery, Hagey Laboratory for Pediatric Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA.

Radiation therapy is effective for cancer treatment but may also result in collateral soft tissue contracture, contour deformities, and non-healing wounds. Autologous fat transfer has been described to improve tissue architecture and function of radiation-induced fibrosis and these effects may be augmented by enrichment with specific adipose-derived stromal cells (ASCs) with enhanced angiogenic potential. CD34+CD146+, CD34+CD146-, or CD34+ unfractionated human ASCs were isolated by flow cytometry and used to supplement human lipoaspirate placed beneath the scalp of irradiated mice. Read More

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December 2021

Corneal Epithelial Stem Cells-Physiology, Pathophysiology and Therapeutic Options.

Cells 2021 09 3;10(9). Epub 2021 Sep 3.

Department of Ophthalmology, University Medical Center, Johannes Gutenberg University Mainz, Langenbeckstrasse 1, 55131 Mainz, Germany.

In the human cornea, regeneration of the epithelium is regulated by the stem cell reservoir of the limbus, which is the marginal region of the cornea representing the anatomical and functional border between the corneal and conjunctival epithelium. In support of this concept, extensive limbal damage, e.g. Read More

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September 2021