J Pharm Biomed Anal 2021 Jun 11;203:114203. Epub 2021 Jun 11.
George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Gh. Marinescu 38, RO-540139, Tîrgu Mureș, Romania; Sz-imfidum Ltd, RO-525401, Lunga No. 504, Romania. Electronic address:
Computer-aided ultrahigh performance liquid chromatographic (UHPLC) method development and optimization was undertaken in order to replace an underperforming European Pharmacopoeia method for the determination of albendazole and its related substances. In the preliminary screening, a temperature-gradient time bidimensional model was chosen to aid selection of the proper stationary phase. Hereinafter temperature-gradient time-ternary composition and temperature-gradient time-pH tridimensional models were applied for the optimization of critical method parameters. Read More